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Items: 8

1.

Multiplex bisulfite sequencing identifies differentially methylated regions in target genes in embryonic cardiomyocytes following knockdown of DNMT1 expression

(Submitter supplied) Purpose: This study aims to identify the differentially methylated regions in target genes in embryonic cardiomyocytes following the knockdown of DNMT1 expression. Methods: Primary cardiomyocytes were isolated from mouse embryonic day (E) 13.5 ventricles, and cultured for 48 h at 37°C to reach 70-80% confluency. Cells were transfected with either Qiagen FlexiTube GeneSolution (with 4 siRNAs) DNMT1 siRNA (#GS13433) or AllStars negative control siRNA (#1027281) at 12 nM using lipofectamine RNAiMAX (n=3 per treatment). more...
Organism:
Mus musculus
Type:
Methylation profiling by high throughput sequencing
Platform:
GPL16417
6 Samples
Download data: TXT
Series
Accession:
GSE81464
ID:
200081464
2.

Illumina MiSeq (Mus musculus)

Platform
Accession:
GPL16417
ID:
100016417
3.

DNMT1_siRNA_BS_rep3

Organism:
Mus musculus
Source name:
Embryonic cardiomyocyte
Platform:
GPL16417
Series:
GSE81464
Download data: TXT
Sample
Accession:
GSM2152815
ID:
302152815
4.

DNMT1_siRNA_BS_rep2

Organism:
Mus musculus
Source name:
Embryonic cardiomyocyte
Platform:
GPL16417
Series:
GSE81464
Download data: TXT
Sample
Accession:
GSM2152814
ID:
302152814
5.

DNMT1_siRNA_BS_rep1

Organism:
Mus musculus
Source name:
Embryonic cardiomyocyte
Platform:
GPL16417
Series:
GSE81464
Download data: TXT
Sample
Accession:
GSM2152813
ID:
302152813
6.

negative_siRNA_BS_rep3 (2)

Organism:
Mus musculus
Source name:
Embryonic cardiomyocyte
Platform:
GPL16417
Series:
GSE81464
Download data: TXT
Sample
Accession:
GSM2152812
ID:
302152812
7.

negative_siRNA_BS_rep2 (2)

Organism:
Mus musculus
Source name:
Embryonic cardiomyocyte
Platform:
GPL16417
Series:
GSE81464
Download data: TXT
Sample
Accession:
GSM2152811
ID:
302152811
8.

negative_siRNA_BS_rep1 (2)

Organism:
Mus musculus
Source name:
Embryonic cardiomyocyte
Platform:
GPL16417
Series:
GSE81464
Download data: TXT
Sample
Accession:
GSM2152810
ID:
302152810
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Supplemental Content

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