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Items: 20

1.

Human DIS3 shapes the RNA polymerase II transcriptome degrading variety of unwanted transcripts.

(Submitter supplied) Human DIS3 is a nuclear, catalytic subunit of the exosome complex containing exonucleolytic and endonucleolytic active domains. To identify DIS3 targets genome-wide we conducted comprehensive transcriptomic analysis of HEK293 cells producing mutated DIS3 versions and Photoactivatable Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation (PAR-CLIP) experiments. Pervasive transcription products like Promoter Upstream Transcripts (PROMPTs) accumulated robustly in catalytic DIS3 mutants, representing ~8% of PAR-CLIP reads. more...
Organism:
Homo sapiens
Type:
Expression profiling by high throughput sequencing; Other; Genome binding/occupancy profiling by high throughput sequencing
Platforms:
GPL18573 GPL18460
17 Samples
Download data: BEDGRAPH, GTF, TSV
2.

Illumina NextSeq 500 (Homo sapiens)

Platform
Accession:
GPL18573
ID:
100018573
3.

Illumina HiSeq 1500 (Homo sapiens)

Platform
Accession:
GPL18460
ID:
100018460
4.

PIN_RNB_Bead_E_PR3

Organism:
Homo sapiens
Source name:
PIN_RNB DIS3 cells Mock control replicate 3
Platform:
GPL18573
Series:
GSE64332
Download data
Sample
Accession:
GSM1820488
ID:
301820488
5.

PIN_RNB_Bead_C_PR2

Organism:
Homo sapiens
Source name:
PIN_RNB DIS3 cells Mock control replicate 2
Platform:
GPL18573
Series:
GSE64332
Download data
Sample
Accession:
GSM1820487
ID:
301820487
6.

PIN_RNB_Bead_PR1

Organism:
Homo sapiens
Source name:
PIN_RNB DIS3 cells Mock control replicate 1
Platform:
GPL18573
Series:
GSE64332
Download data
Sample
Accession:
GSM1820486
ID:
301820486
7.

PIN_RNB_IP_E_PR3

Organism:
Homo sapiens
Source name:
PIN_RNB DIS3 cells RNA Pol II CHIP replicate 3
Platform:
GPL18573
Series:
GSE64332
Download data
Sample
Accession:
GSM1820485
ID:
301820485
8.

PIN_RNB_IP_C_PR2

Organism:
Homo sapiens
Source name:
PIN_RNB DIS3 cells RNA Pol II CHIP replicate 2
Platform:
GPL18573
Series:
GSE64332
Download data
Sample
Accession:
GSM1820484
ID:
301820484
9.

PIN_RNB_IP_PR1

Organism:
Homo sapiens
Source name:
PIN_RNB DIS3 cells RNA Pol II CHIP replicate 1
Platform:
GPL18573
Series:
GSE64332
Download data
Sample
Accession:
GSM1820483
ID:
301820483
10.

WT_Bead_E_PR3

Organism:
Homo sapiens
Source name:
WT DIS3 cells Mock control replicate 3
Platform:
GPL18573
Series:
GSE64332
Download data
Sample
Accession:
GSM1820482
ID:
301820482
11.

WT_Bead_C_PR2

Organism:
Homo sapiens
Source name:
WT DIS3 cells Mock control replicate 2
Platform:
GPL18573
Series:
GSE64332
Download data
Sample
Accession:
GSM1820481
ID:
301820481
12.

hDIS3_PAR-CLIP

Organism:
Homo sapiens
Source name:
HEK293 Flip-In T-Rex cell line
Platform:
GPL18460
Series:
GSE64332
Download data: BEDGRAPH
Sample
Accession:
GSM1568713
ID:
301568713
13.

WT_Bead_PR1

Organism:
Homo sapiens
Source name:
WT DIS3 cells Mock control replicate 1
Platform:
GPL18573
Series:
GSE64332
Download data
Sample
Accession:
GSM1820480
ID:
301820480
14.

hDIS3_PIN_RNB_mut_RNA-seq

Organism:
Homo sapiens
Source name:
HEK293 Flip-In T-Rex cell line
Platform:
GPL18460
Series:
GSE64332
Download data: TSV
Sample
Accession:
GSM1568712
ID:
301568712
15.

WT_IP_E_PR3

Organism:
Homo sapiens
Source name:
WT DIS3 cells RNA Pol II CHIP replicate 3
Platform:
GPL18573
Series:
GSE64332
Download data
Sample
Accession:
GSM1820479
ID:
301820479
16.

hDIS3_RNB_mut_RNA-seq

Organism:
Homo sapiens
Source name:
HEK293 Flip-In T-Rex cell line
Platform:
GPL18460
Series:
GSE64332
Download data: TSV
Sample
Accession:
GSM1568711
ID:
301568711
17.

WT_IP_C_PR2

Organism:
Homo sapiens
Source name:
WT DIS3 cells RNA Pol II CHIP replicate 2
Platform:
GPL18573
Series:
GSE64332
Download data
Sample
Accession:
GSM1820478
ID:
301820478
18.

hDIS3_PIN_mut_RNA-seq

Organism:
Homo sapiens
Source name:
HEK293 Flip-In T-Rex cell line
Platform:
GPL18460
Series:
GSE64332
Download data: TSV
Sample
Accession:
GSM1568710
ID:
301568710
19.

WT_IP_PR1

Organism:
Homo sapiens
Source name:
WT DIS3 cells RNA Pol II CHIP replicate 1
Platform:
GPL18573
Series:
GSE64332
Download data
Sample
Accession:
GSM1820477
ID:
301820477
20.

hDIS3_WT_RNA-seq

Organism:
Homo sapiens
Source name:
HEK293 Flip-In T-Rex cell line
Platform:
GPL18460
Series:
GSE64332
Download data: TSV
Sample
Accession:
GSM1568709
ID:
301568709
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Supplemental Content

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