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Items: 6

1.

Relocalization of retinoic acid receptors from non-canonical to canonical spaced binding elements during embryoid body differentiation

(Submitter supplied) Retinoic Acid Receptors (RARs) bind RA-response elements in regulatory regions of their target genes. While canonical RAREs comprise direct repeats of the consensus 5’-RGKTCA-3’ sequence separated by 1, 2 or 5 nucleotides (DR1, DR2, DR5), we show that shortly after RA treatement of mouse embryoid bodies or F9 cells, RARs occupy a large repertoire of DR0, DR2, DR5, DR8 and IR0 elements. In vitro, RAR-RXR bind these non-canonical spacings with comparable affinities to DR2 and DR5. more...
Organism:
Mus musculus
Type:
Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL11002
4 Samples
Download data: BED
Series
Accession:
GSE35599
ID:
200035599
2.

Illumina Genome Analyzer IIx (Mus musculus)

Platform
Accession:
GPL11002
ID:
100011002
3.

Control RNASeq

Organism:
Mus musculus
Source name:
murine embryonic stem cells grown as embryoid bodies for 4 days with no retinoic acid treatment
Platform:
GPL11002
Series:
GSE35599
Download data
Sample
Accession:
GSM871398
ID:
300871398
4.

RA 24h RNASeq

Organism:
Mus musculus
Source name:
murine embryonic stem cells grown as embryoid bodies for 4 days and treated 24 hours with 5 micromolar all trans retinoic acid
Platform:
GPL11002
Series:
GSE35599
Download data
Sample
Accession:
GSM871397
ID:
300871397
5.

RA 24h ChIPSeq

Organism:
Mus musculus
Source name:
murine embryonic stem cells grown as embryoid bodies for 4 days and treated 24 hours with 5 micromolar all trans retinoic acid
Platform:
GPL11002
Series:
GSE35599
Download data: BED
Sample
Accession:
GSM871396
ID:
300871396
6.

RA 2h ChIPSeq

Organism:
Mus musculus
Source name:
murine embryonic stem cells grown as embryoid bodies for 4 days and treated 2 hours with 5 micromolar all trans retinoic acid
Platform:
GPL11002
Series:
GSE35599
Download data: BED
Sample
Accession:
GSM871395
ID:
300871395
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Supplemental Content

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