GSE224149[Accession] - GEO DataSets - NCBI

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Items: 19

Select item 2002241491.

Scalable co-sequencing of RNA and DNA from individual nuclei

(Submitter supplied) We present DEFND-seq (DNA and Expression From Nucleosome Depletion), a scalable method for co-sequencing RNA and DNA from single nuclei. In DEFND-seq we treat nuclei with lithium diiodosalicylate to disrupt chromatin and expose genoimc DNA. The nuclei are then tagmented with Tn5 transposase, which fragments and tags gDNA. Tagmented nuclei are loaded into a microfluidic droplet generator which combines nuclei, beads containing transcriptomic and genomic barcodes, and reverse transcription reagents into single droplets. more...

Organism:: Homo sapiens; Mus musculus

Type:: Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing; Other

5 related Platforms
13 Samples

Download data: TSV, TXT

Series

Accession:: GSE224149

ID:: 200224149

PubMed Full text in PMC Similar studies

Select item 1000344742.

Element AVITI (Homo sapiens; Mus musculus)

Organism:: Homo sapiens; Mus musculus

1 Series
2 Samples

Platform

Accession:: GPL34474

ID:: 100034474

Select item 1000246763.

Illumina NovaSeq 6000 (Homo sapiens)

Organism:: Homo sapiens

16543 Series
922800 Samples

Platform

Accession:: GPL24676

ID:: 100024676

Select item 1000246254.

NextSeq 550 (Homo sapiens; Mus musculus)

Organism:: Homo sapiens; Mus musculus

23 Series
84 Samples

Platform

Accession:: GPL24625

ID:: 100024625

Select item 1000216975.

NextSeq 550 (Homo sapiens)

Organism:: Homo sapiens

1460 Series
43598 Samples

Platform

Accession:: GPL21697

ID:: 100021697

Select item 1000194156.

Illumina NextSeq 500 (Homo sapiens; Mus musculus)

Organism:: Homo sapiens; Mus musculus

97 Series
1663 Samples

Platform

Accession:: GPL19415

ID:: 100019415

Select item 3082652907.

snRNA-seq of LAND-treated U87 cells and 3T3 cells with 10x Multiome (DEFND-seq RNA library - second reaction)

Organism:: Homo sapiens; Mus musculus

Source name:: U87-MG, NIH-3T3

Platform:: GPL19415
Series:: GSE224149

Sample

Accession:: GSM8265290

ID:: 308265290

SRA Run Selector

Select item 3082652898.

snRNA-seq of LAND-treated U87 cells and 3T3 cells with 10x Multiome (DEFND-seq RNA library - first reaction)

Organism:: Homo sapiens; Mus musculus

Source name:: U87-MG, NIH-3T3

Platform:: GPL19415
Series:: GSE224149

Download data: TXT

Sample

Accession:: GSM8265289

ID:: 308265289

SRA Run Selector

Select item 3082652929.

snDNA-seq of LAND-treated U87 cells and 3T3 cells with 10x Multiome (DEFND-seq DNA library - second reaction)

Organism:: Mus musculus; Homo sapiens

Source name:: U87-MG, NIH-3T3

Platform:: GPL34474
Series:: GSE224149

Download data: TSV

Sample

Accession:: GSM8265292

ID:: 308265292

SRA Run Selector

Select item 30826529110.

snDNA-seq of LAND-treated U87 cells and 3T3 cells with 10x Multiome (DEFND-seq DNA library - first reaction)

Organism:: Mus musculus; Homo sapiens

Source name:: U87-MG, NIH-3T3

Platform:: GPL34474
Series:: GSE224149

Download data: TSV

Sample

Accession:: GSM8265291

ID:: 308265291

SRA Run Selector

Select item 30781778011.

snRNA-seq of LAND-treated glioblastoma nuclei with 10x Multiome (DEFND-seq RNA library) - PTO037

Organism:: Homo sapiens

Source name:: glioblastoma tissue

Platform:: GPL24676
Series:: GSE224149

Download data: TXT

Sample

Accession:: GSM7817780

ID:: 307817780

SRA Run Selector

Select item 30701641812.

snDNA-seq of LAND-treated U87 and 3T3 nuclei with 10x Multiome (DEFND-seq DNA library)

Organism:: Homo sapiens; Mus musculus

Source name:: U87 human glioma cell line / 3T3 mouse fibroblast cell line

Platform:: GPL24625
Series:: GSE224149

Download data: TSV

Sample

Accession:: GSM7016418

ID:: 307016418

SRA Run Selector

Select item 30701641713.

snRNA-seq of LAND-treated U87 and 3T3 nuclei with 10x Multiome (DEFND-seq RNA library)

Organism:: Mus musculus; Homo sapiens

Source name:: U87 human glioma cell line / 3T3 mouse fibroblast cell line

Platform:: GPL24625
Series:: GSE224149

Download data: TXT

Sample

Accession:: GSM7016417

ID:: 307016417

SRA Run Selector

Select item 30701641514.

snRNA-seq of LAND-treated BJ nuclei with 10x Multiome (DEFND-seq RNA library)

Organism:: Homo sapiens

Source name:: BJ fibroblast cell line

Platform:: GPL21697
Series:: GSE224149

Download data: TXT

Sample

Accession:: GSM7016415

ID:: 307016415

SRA Run Selector

Select item 30701641415.

snATAC-seq of BJ nuclei with 10x scATAC-seq

Organism:: Homo sapiens

Source name:: BJ fibroblast cell line

Platform:: GPL21697
Series:: GSE224149

Download data: TSV

Sample

Accession:: GSM7016414

ID:: 307016414

SRA Run Selector

Select item 30701641316.

snDNA-seq of xSDS-treated BJ nuclei with 10x scATAC-seq kit

Organism:: Homo sapiens

Source name:: BJ fibroblast cell line

Platform:: GPL21697
Series:: GSE224149

Download data: TSV

Sample

Accession:: GSM7016413

ID:: 307016413

SRA Run Selector

Select item 30701641217.

snDNA-seq of LAND-treated BJ nuclei with 10x scATAC-seq kit

Organism:: Homo sapiens

Source name:: BJ fibroblast cell line

Platform:: GPL21697
Series:: GSE224149

Download data: TSV

Sample

Accession:: GSM7016412

ID:: 307016412

SRA Run Selector

Select item 30701641918.

snRNA-seq of LAND-treated glioblastoma nuclei with 10x Multiome (DEFND-seq RNA library)

Organism:: Homo sapiens

Source name:: glioblastoma tissue

Platform:: GPL24676
Series:: GSE224149

Download data: TXT

Sample

Accession:: GSM7016419

ID:: 307016419

SRA Run Selector

Select item 30701641619.

snDNA-seq of LAND-treated BJ nuclei with 10x Multiome (DEFND-seq DNA library)

Organism:: Homo sapiens

Source name:: BJ fibroblast cell line

Platform:: GPL24676
Series:: GSE224149

Download data: TSV

Sample

Accession:: GSM7016416

ID:: 307016416

SRA Run Selector

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