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Items: 1 to 20 of 131

1.

Hammer-seq to measure kinetics of DNA methylation maintenance in HeLa cells

(Submitter supplied) Simultaneous measurement of the methylation status of parent and daughter strands of newly replicated DNA at the single molecule level has not been experimentally achieved, which is essential for measurements the kinetics of DNA methylation maintenance at base level genome wide. To achieve this, we developed a new method termed Hammer-seq (Hairpin assisted mapping of methylation of replicated DNA), which combines EdU labeling, biotin conjugation, immunopurification and hairpin bisulfite sequencing technologies. more...
Organism:
Homo sapiens; Mus musculus
Type:
Methylation profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing
4 related Platforms
126 Samples
Download data: BIGWIG, TXT
Series
Accession:
GSE131098
ID:
200131098
2.

Illumina NovaSeq 6000 (Homo sapiens)

Platform
Accession:
GPL24676
ID:
100024676
3.

Illumina NovaSeq 6000 (Mus musculus)

Platform
Accession:
GPL24247
ID:
100024247
4.

HiSeq X Ten (Mus musculus)

Platform
Accession:
GPL21273
ID:
100021273
5.

HiSeq X Ten (Homo sapiens)

Platform
Accession:
GPL20795
ID:
100020795
6.

10h-IAA-14hR-rescue, replicate

Organism:
Homo sapiens
Source name:
10 hr IAA treatment of UHRF1-AID HeLa cells, withdraw IAA, then release 14 h (rescue), replicate
Platform:
GPL24676
Series:
GSE131098
Download data: TXT
Sample
Accession:
GSM4633262
ID:
304633262
7.

10h-IAA-6hR-rescue, replicate

Organism:
Homo sapiens
Source name:
10 hr IAA treatment of UHRF1-AID HeLa cells, withdraw IAA, then release 6 h (rescue), replicate
Platform:
GPL24676
Series:
GSE131098
Download data: TXT
Sample
Accession:
GSM4633261
ID:
304633261
8.

10h-IAA-4hR-rescue, replicate

Organism:
Homo sapiens
Source name:
10 hr IAA treatment of UHRF1-AID HeLa cells, withdraw IAA, then release 4 h (rescue), replicate
Platform:
GPL24676
Series:
GSE131098
Download data: TXT
Sample
Accession:
GSM4633260
ID:
304633260
9.

10h-IAA-2hR-rescue, replicate

Organism:
Homo sapiens
Source name:
10 hr IAA treatment of UHRF1-AID HeLa cells, withdraw IAA, then release 2 h (rescue), replicate
Platform:
GPL24676
Series:
GSE131098
Download data: TXT
Sample
Accession:
GSM4633259
ID:
304633259
10.

10h-IAA-0hR-rescue, replicate

Organism:
Homo sapiens
Source name:
10 hr IAA treatment of UHRF1-AID HeLa cells, withdraw IAA, then release 0 h (rescue), replicate
Platform:
GPL24676
Series:
GSE131098
Download data: TXT
Sample
Accession:
GSM4633258
ID:
304633258
11.

RescueTo24h-noIAA, replicate

Organism:
Homo sapiens
Source name:
no IAA treatment of UHRF1-AID HeLa cells, release 24 h, replicate.
Platform:
GPL24676
Series:
GSE131098
Download data: TXT
Sample
Accession:
GSM4633257
ID:
304633257
12.

DNMT3A3B-DKO-chase-24h

Organism:
Homo sapiens
Source name:
EdU labeled DNMT3A/3B double KO HeLa cells with chasing 24 h
Platform:
GPL24676
Series:
GSE131098
Download data: TXT
Sample
Accession:
GSM4633256
ID:
304633256
13.

DNMT3A3B-DKO-chase-0, replicate

Organism:
Homo sapiens
Source name:
EdU labeled DNMT3A/3B double KO HeLa cells with chasing 0 min, replicate
Platform:
GPL24676
Series:
GSE131098
Download data: TXT
Sample
Accession:
GSM4633255
ID:
304633255
14.

LSH-AID-late-chase-24h, replicate

Organism:
Homo sapiens
Source name:
IAA-treated LSH-AID HeLa cells, release 6 hr, EdU labeled and chase 24 hr, replicate
Platform:
GPL24676
Series:
GSE131098
Download data: TXT
Sample
Accession:
GSM4633254
ID:
304633254
15.

LSH-AID-late-chase-2h, replicate

Organism:
Homo sapiens
Source name:
IAA-treated LSH-AID HeLa cells, release 6 hr, EdU labeled and chase 2 hr, replicate
Platform:
GPL24676
Series:
GSE131098
Download data: TXT
Sample
Accession:
GSM4633253
ID:
304633253
16.

LSH-AID-late-chase-30m, replicate

Organism:
Homo sapiens
Source name:
IAA-treated LSH-AID HeLa cells, release 6 hr, EdU labeled and chase 30 min, replicate
Platform:
GPL24676
Series:
GSE131098
Download data: TXT
Sample
Accession:
GSM4633252
ID:
304633252
17.

LSH-AID-late-chase-5m, replicate

Organism:
Homo sapiens
Source name:
IAA-treated LSH-AID HeLa cells, release 6 hr, EdU labeled and chase 5 min, replicate
Platform:
GPL24676
Series:
GSE131098
Download data: TXT
Sample
Accession:
GSM4633251
ID:
304633251
18.

LSH-AID-late-chase-0, replicate

Organism:
Homo sapiens
Source name:
IAA-treated LSH-AID HeLa cells, release 6 hr, EdU labeled and chase 0 min, replicate
Platform:
GPL24676
Series:
GSE131098
Download data: TXT
Sample
Accession:
GSM4633250
ID:
304633250
19.

LSH-AID-chase-24h, replicate

Organism:
Homo sapiens
Source name:
EdU labeled IAA-treated LSH-AID HeLa cells with chasing 24 hr, replicate
Platform:
GPL24676
Series:
GSE131098
Download data: TXT
Sample
Accession:
GSM4633249
ID:
304633249
20.

LSH-AID-chase-2h, replicate

Organism:
Homo sapiens
Source name:
EdU labeled IAA-treated LSH-AID HeLa cells with chasing 2 hr, replicate
Platform:
GPL24676
Series:
GSE131098
Download data: TXT
Sample
Accession:
GSM4633248
ID:
304633248
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Supplemental Content

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