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Items: 17

1.

m7G methylation by METTL1 regulates let-7 microRNAs [BoRed-Seq & m7G-RIP-Seq A549]

(Submitter supplied) Methylation of N7-methylguanosine (m7G) is found at mRNA caps and at defined internal positions within abundant tRNAs and rRNAs. However, its detection within low abundance mRNAs and microRNAs (miRNAs) has been hampered by lack of sensitive detection strategies. Here, we adapt a chemical reactivity assay to detect internal m7G in RNA from eukaryotic cells. Using this approach, alongside a confirmational RNA immunoprecipitation assay, we identify m7G within miRNAs inhibiting cell migration, and show that METTL1 mediates this m7G methylation. more...
Organism:
Homo sapiens
Type:
Non-coding RNA profiling by high throughput sequencing; Other
Platforms:
GPL18460 GPL20301
14 Samples
Download data: TXT
Series
Accession:
GSE112181
ID:
200112181
2.

Illumina HiSeq 4000 (Homo sapiens)

Platform
Accession:
GPL20301
ID:
100020301
3.

Illumina HiSeq 1500 (Homo sapiens)

Platform
Accession:
GPL18460
ID:
100018460
4.

A549 m7G-RIP IP Rep 5

Organism:
Homo sapiens
Source name:
A549 human lung adenocarcinoma cell line
Platform:
GPL20301
Series:
GSE112181 GSE112182
Download data
Sample
Accession:
GSM3059558
ID:
303059558
5.

A549 m7G-RIP IP Rep 4

Organism:
Homo sapiens
Source name:
A549 human lung adenocarcinoma cell line
Platform:
GPL20301
Series:
GSE112181 GSE112182
Download data
Sample
Accession:
GSM3059557
ID:
303059557
6.

A549 m7G-RIP IP Rep 3

Organism:
Homo sapiens
Source name:
A549 human lung adenocarcinoma cell line
Platform:
GPL20301
Series:
GSE112181 GSE112182
Download data
Sample
Accession:
GSM3059556
ID:
303059556
7.

A549 m7G-RIP IP Rep 2

Organism:
Homo sapiens
Source name:
A549 human lung adenocarcinoma cell line
Platform:
GPL20301
Series:
GSE112181 GSE112182
Download data
Sample
Accession:
GSM3059555
ID:
303059555
8.

A549 m7G-RIP IP Rep 1

Organism:
Homo sapiens
Source name:
A549 human lung adenocarcinoma cell line
Platform:
GPL20301
Series:
GSE112181 GSE112182
Download data
Sample
Accession:
GSM3059554
ID:
303059554
9.

A549 m7G-RIP Input Rep 5

Organism:
Homo sapiens
Source name:
A549 human lung adenocarcinoma cell line
Platform:
GPL20301
Series:
GSE112181 GSE112182
Download data
Sample
Accession:
GSM3059553
ID:
303059553
10.

A549 m7G-RIP Input Rep 4

Organism:
Homo sapiens
Source name:
A549 human lung adenocarcinoma cell line
Platform:
GPL20301
Series:
GSE112181 GSE112182
Download data
Sample
Accession:
GSM3059552
ID:
303059552
11.

A549 m7G-RIP Input Rep 3

Organism:
Homo sapiens
Source name:
A549 human lung adenocarcinoma cell line
Platform:
GPL20301
Series:
GSE112181 GSE112182
Download data
Sample
Accession:
GSM3059551
ID:
303059551
12.

A549 m7G-RIP Input Rep 2

Organism:
Homo sapiens
Source name:
A549 human lung adenocarcinoma cell line
Platform:
GPL20301
Series:
GSE112181 GSE112182
Download data
Sample
Accession:
GSM3059550
ID:
303059550
13.

A549 m7G-RIP Input Rep 1

Organism:
Homo sapiens
Source name:
A549 human lung adenocarcinoma cell line
Platform:
GPL20301
Series:
GSE112181 GSE112182
Download data
Sample
Accession:
GSM3059549
ID:
303059549
14.

A549 BoRed-Seq Red Rep 2

Organism:
Homo sapiens
Source name:
A549 human lung adenocarcinoma cell line
Platform:
GPL18460
Series:
GSE112181 GSE112182
Download data
Sample
Accession:
GSM3059548
ID:
303059548
15.

A549 BoRed-Seq Red Rep 1

Organism:
Homo sapiens
Source name:
A549 human lung adenocarcinoma cell line
Platform:
GPL18460
Series:
GSE112181 GSE112182
Download data
Sample
Accession:
GSM3059547
ID:
303059547
16.

A549 BoRed-Seq Ctrl Rep 2

Organism:
Homo sapiens
Source name:
A549 human lung adenocarcinoma cell line
Platform:
GPL18460
Series:
GSE112181 GSE112182
Download data
Sample
Accession:
GSM3059546
ID:
303059546
17.

A549 BoRed-Seq Ctrl Rep 1

Organism:
Homo sapiens
Source name:
A549 human lung adenocarcinoma cell line
Platform:
GPL18460
Series:
GSE112181 GSE112182
Download data
Sample
Accession:
GSM3059545
ID:
303059545
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Supplemental Content

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