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Items: 1 to 20 of 911

1.

Cellular responses toatmospheric-pressure plasma in fission yeast

(Submitter supplied) We characterized fission yeast cellular responses to a novel stress inducer, non-thermal atmospheric-pressure plasma.
Organism:
Schizosaccharomyces pombe
Type:
Expression profiling by high throughput sequencing
Platform:
GPL13988
15 Samples
Download data: TXT
Series
Accession:
GSE230503
ID:
200230503
2.

Topoisomerase 1 facilitates nucleosome reassembly at stress genes during recovery.

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Schizosaccharomyces pombe
Type:
Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing
Platforms:
GPL13988 GPL20584
24 Samples
Download data: BW
Series
Accession:
GSE227184
ID:
200227184
3.

Topoisomerase 1 facilitates nucleosome reassembly at stress genes during recovery [RNA-seq]

(Submitter supplied) Investigate the role of topoisomerase 1 in the response to oxidative stress
Organism:
Schizosaccharomyces pombe
Type:
Expression profiling by high throughput sequencing
Platform:
GPL13988
8 Samples
Download data: TXT
Series
Accession:
GSE227183
ID:
200227183
4.

Investigation of mettl4 mediated alterative splicing variation

(Submitter supplied) We compare mettl4 KO and WT samples from different species to understand how mettl4 affects the alterative splicing
Organism:
Schizosaccharomyces pombe; Danio rerio; Homo sapiens; Drosophila melanogaster; Mus musculus
Type:
Expression profiling by high throughput sequencing
5 related Platforms
23 Samples
Download data: MATRIX
Series
Accession:
GSE178511
ID:
200178511
5.

The evolution, evolvability, and engineering of gene regulatory DNA [RNA-seq]

(Submitter supplied) Here, we used RNA-seq to quantify expression conservation across many Ascomycete yeasts.
Organism:
Schizosaccharomyces pombe; Nakaseomyces glabratus; Kluyveromyces lactis; Lachancea waltii; Saccharomyces cerevisiae; Candida albicans; Schizosaccharomyces japonicus; Schizosaccharomyces octosporus; Saccharomyces bayanus; Yarrowia lipolytica; Naumovozyma castellii
Type:
Expression profiling by high throughput sequencing
11 related Platforms
11 Samples
Download data: TXT
Series
Accession:
GSE163866
ID:
200163866
6.

The histone chaperone FACT facilitates heterochromatin spreading through regulation of histone turnover and H3K9 methylation states

(Submitter supplied) Heterochromatin formation requires several steps involving: nucleation followed by spreading of large, silent domains and its faithful re-establishment and maintenance after each replication cycle. The essential histone chaperone FACT has been implicated in heterochromatin silencing, however, little is known about the specificity of FACT in this process. Here, by applying single cell assays, ChIP and genetics, we show that FACT mutants have defects in the spreading of heterochromatin at subtelomeres and mating type locus in fission yeast. more...
Organism:
Schizosaccharomyces pombe
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL13988
8 Samples
Download data: BW
Series
Accession:
GSE174641
ID:
200174641
7.

U2AF65Prp2 promotes intron definition by stabilizing U1 snRNP at the 5’ splice site

(Submitter supplied) Prp2 (U2AF65) is an essential splicing factor, that recognizes the poly-Py track near the 3' SS. We have previously observed that when Prp2 is inactivated using a prp2 temperature-sensitive allele, while most of the pre-mRNA is not processed, some are normally spliced. This observation even more noticeable during meiosis, since pre-mRNA regulation is executed at different levels (splicing, polyadenylation and stability). more...
Organism:
Schizosaccharomyces pombe
Type:
Expression profiling by high throughput sequencing
Platform:
GPL13988
2 Samples
Download data: XLSX
Series
Accession:
GSE97203
ID:
200097203
8.

Small RNA sequencing of the 8 to 30-nucleotide window from various samples

(Submitter supplied) RNA-sequencing (RNA-Seq) protocols and bioinformatic pipelines are designed to streamline downstream analyses on sequences believed to be the most important. Here, we have challenged this dogma by preserving ribosomal RNA (rRNA) in our samples and by lowering the minimal RNA size window of our small RNA-Seq analyses to 8 nt
Organism:
Arabidopsis thaliana; Homo sapiens; Mus musculus; Drosophila melanogaster; Saccharomyces cerevisiae; Schizosaccharomyces pombe
Type:
Non-coding RNA profiling by high throughput sequencing
6 related Platforms
11 Samples
Download data: FA
Series
Accession:
GSE179677
ID:
200179677
9.

Transcriptome sequencing of genes related to glucose availability in Schizosaccharomyces pombe by RNA-seq analysis

(Submitter supplied) Gene expression profiles in Schizosaccharomyces pombe mid-log-phase cells grown in three different Synthetic Dextrose medium with different glucose content were compared using the RNA sequencing method. Expression patterns of genes that function in carbohydrate metabolism were downregulated as expected, and these genes were downregulated in line with the increase in glucose content. Significant and consistent changes in the expression were observed such as genes that encoding retrotransposable elements, heat shock proteins, glutathione S-transferase, cell agglutination protein, and conserved fungal proteins.
Organism:
Schizosaccharomyces pombe
Type:
Expression profiling by high throughput sequencing
Platform:
GPL13988
3 Samples
Download data: XLSX
Series
Accession:
GSE159723
ID:
200159723
10.

Transcriptional profiling of fission yeast RNA polymerase II CTD mutants

(Submitter supplied) This RNA-Seq analysis compares gene expression of the RNA polymerase II CTD-mutants contrasting to the WT RNA polymerase II CTD (with isogenic 3' selection marker) in the fission yeast S. pombe
Organism:
Schizosaccharomyces pombe
Type:
Expression profiling by high throughput sequencing
Platforms:
GPL13988 GPL28961
29 Samples
Download data: TXT, XLSX
Series
Accession:
GSE160852
ID:
200160852
11.

Epigenetic gene silencing by heterochromatin primes fungal resistance

(Submitter supplied) Genes embedded in H3 lysine 9 methylation (H3K9me)-dependent heterochromatin are transcriptionally silenced. In fission yeast, Schizosaccharomyces pombe, H3K9me-mediated heterochromatin silencing can be transmitted through cell division provided the counteracting demethylase Epe1 is absent. It is possible that under certain conditions wild-type cells might utilize heterochromatin heritability to form epimutations, phenotypes mediated by unstable silencing rather than DNA changes. more...
Organism:
Schizosaccharomyces pombe
Type:
Genome binding/occupancy profiling by high throughput sequencing; Non-coding RNA profiling by high throughput sequencing; Expression profiling by high throughput sequencing
4 related Platforms
49 Samples
Download data: BW, CSV
Series
Accession:
GSE138436
ID:
200138436
12.

The roles of duplication and divergence in the evolution of a transcription factor

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Naumovozyma castellii; Saccharomyces paradoxus; Saccharomyces cerevisiae; Kluyveromyces lactis; Nakaseomyces glabratus; Schizosaccharomyces pombe
Type:
Genome binding/occupancy profiling by high throughput sequencing; Expression profiling by high throughput sequencing
10 related Platforms
128 Samples
Download data: BW
Series
Accession:
GSE119238
ID:
200119238
13.

SFP1 (RNA-seq)

(Submitter supplied) We measured global expression profiles in SFP1 deletion mutants and isogenic wild type strains during lag and log phase growth. To match the physiology of each species as well as minimize the effect on gene expression from differences in early growth rates, we profiled expression during log growth and lag after glucose repletion as previously described (Thompson et al., 2013).
Organism:
Naumovozyma castellii; Saccharomyces paradoxus; Schizosaccharomyces pombe; Nakaseomyces glabratus; Saccharomyces cerevisiae; Kluyveromyces lactis
Type:
Expression profiling by high throughput sequencing
6 related Platforms
58 Samples
Download data: TXT
Series
Accession:
GSE119237
ID:
200119237
14.

Cdk9 and H2Bub1 signal to Clr6-CII/Rpd3S to suppress aberrant antisense transcription

(Submitter supplied) Histone H2B mono-ubiquitylation (H2Bub1) and phosphorylation of elongation factor Spt5 by cyclin-dependent kinase 9 (Cdk9) occur during transcription by RNA polymerase II (RNAPII), and are mutually dependent in fission yeast. How Cdk9 activity and H2Bub1 cooperate to regulate the expression of individual genes remains unclear. Here we show Cdk9 inhibition or H2Bub1 loss induces intragenic antisense transcription of distinct gene subsets; ablation of both pathways derepresses antisense transcription of over half the genome. more...
Organism:
Schizosaccharomyces pombe
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platforms:
GPL20584 GPL13988
24 Samples
Download data: BIGWIG
Series
Accession:
GSE115682
ID:
200115682
15.

Abo1 is required for H3K9me2 to H3K9me3 transition in telomeric and centromeric heterochromatin

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Schizosaccharomyces pombe
Type:
Expression profiling by genome tiling array; Genome binding/occupancy profiling by high throughput sequencing
Platforms:
GPL13988 GPL7715
20 Samples
Download data: BEDGRAPH, CEL
Series
Accession:
GSE125912
ID:
200125912
16.

Abo1 is required for H3K9me2 to H3K9me3 transition in telomeric and centromeric heterochromatin [ChIP-seq]

(Submitter supplied) Regulation of heterochromatin is critical for genome stability. Different states of methylated H3K9 have been discovered with distinct roles in heterochromatin formation and silencing. However, the control of the transition from H3K9me2 to H3K9me3 is still unclear. Here we investigate the role of the conserved bromodomain AAA-ATPase, Abo1, involved in maintaining the global nucleosome organization in fission yeast. more...
Organism:
Schizosaccharomyces pombe
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL13988
8 Samples
Download data: BEDGRAPH
Series
Accession:
GSE125911
ID:
200125911
17.

Effect of D2O on S. pombe gene expression

(Submitter supplied) RNA sequencing of total RNA purified from wild type S. pombe cells after 0, 5 and 24 hours culture in YES containing 100% D2O (deuterium oxide).
Organism:
Schizosaccharomyces pombe
Type:
Expression profiling by high throughput sequencing
Platform:
GPL13988
12 Samples
Download data: TXT
Series
Accession:
GSE119785
ID:
200119785
18.

The chaperone FACT and H2B ubiquitination maintain S. pombe genome architecture through genic and subtelomeric functions [ChIP-Seq]

(Submitter supplied) The histone chaperone FACT and histone H2B ubiquitination (H2Bub) facilitate RNA Polymerase II passage through chromatin. It remains unknown to what extent they cooperate in vivo. Here, we used genome-wide, genetic, biochemical and microscopic approaches to dissect their interplay in the fission yeast Schizosaccharomyces pombe. We show that FACT and H2Bub repress antisense transcripts near the 5’ end and inside gene bodies, respectively. more...
Organism:
Schizosaccharomyces pombe
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL13988
44 Samples
Download data: BEDGRAPH
Series
Accession:
GSE135766
ID:
200135766
19.

The chaperone FACT and H2B ubiquitination maintain S. pombe genome architecture through genic and subtelomeric functions

(Submitter supplied) This SuperSeries is composed of the SubSeries listed below.
Organism:
Schizosaccharomyces pombe
Type:
Expression profiling by high throughput sequencing; Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL13988
84 Samples
Download data: BEDGRAPH, BW
Series
Accession:
GSE124092
ID:
200124092
20.

The chaperone FACT and H2B ubiquitination maintain S. pombe genome architecture through genic and subtelomeric functions [MNase-seq]

(Submitter supplied) The histone chaperone FACT and histone H2B ubiquitination (H2Bub) facilitate RNA Polymerase II passage through chromatin. It remains unknown to what extent they cooperate in vivo. Here, we used genome-wide, genetic, biochemical and microscopic approaches to dissect their interplay in the fission yeast Schizosaccharomyces pombe. We show that FACT and H2Bub repress antisense transcripts near the 5’ end and inside gene bodies, respectively. more...
Organism:
Schizosaccharomyces pombe
Type:
Genome binding/occupancy profiling by high throughput sequencing
Platform:
GPL13988
32 Samples
Download data: BW
Series
Accession:
GSE124091
ID:
200124091
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