2V5Q,2OU7,3D5W,3KB7


Conserved Protein Domain Family
STKc_PLK1

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cd14187: STKc_PLK1 
Click on image for an interactive view with Cn3D
Catalytic domain of the Serine/Threonine Kinase, Polo-like kinase 1
STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. PLKs play important roles in cell cycle progression and in DNA damage responses. They regulate mitotic entry, mitotic exit, and cytokinesis. In general PLKs contain an N-terminal catalytic kinase domain and a C-terminal regulatory polo box domain (PBD), which is comprised by two bipartite polo-box motifs (or polo boxes) and is involved in protein interactions. There are five mammalian PLKs (PLK1-5) from distinct genes. PLK1 functions as a positive regulator of mitosis, meiosis, and cytokinesis. Its localization changes during mitotic progression; associating first with centrosomes in prophase, with kinetochores in prometaphase and metaphase, at the central spindle in anaphase, and in the midbody during telophase. It carries multiple functions throughout the cell cycle through interactions with differrent substrates at these specific subcellular locations. PLK1 is overexpressed in many human cancers and is associated with poor prognosis. The PLK1 subfamily is part of a larger superfamily that includes the catalytic domains of other STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase.
Statistics
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PSSM-Id: 271089
Aligned: 6 rows
Threshold Bit Score: 546.839
Created: 31-Aug-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 15 residues -Click on image for an interactive view with Cn3D
Feature 1:ATP binding site [chemical binding site]
Evidence:
  • Structure:2OU7: Human PLK1 binds Mg-ANP; contacts at 4A.
  • Structure:3D5W: Danio rerio PLK1 binds ADP; contacts at 4A.
  • Structure:3KB7: Human PLK1 binds a pyrazoloquinazoline inhibitor; contacts at 4A.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                      ####    #            # #                               #          
2V5Q_A        15 VDPRSRRRYVRGRfLGKGGFAKCFEISDAdtKEVFAGKIVPKSLLlKPHQREKMSMEISIHRSLaHQHVVGFHGFFEDnd 94  human
2OU7_A        35 VDPRSRRRYVRGRfLGKGGFAKCFEISDAdtKEVFAGKIVPKSLLlKPHQREKMSMEISIHRSLaHQHVVGFHGFFEDnd 114 human
3D5W_A        36 VDPRTMKRYMRGRfLGKGGFAKCYEITDMdtKEVFAGKVVPKSMLlKPHQKEKMSTEIAIHKSLdNPHVVGFHGFFEDdd 115 zebrafish
3KB7_A        11 VDPRSRRRYVRGRfLGKGGFAKCFEISDAdtKEVFAGKIVPKSLLlKPHQREKMSMEISIHRSLaHQHVVGFHGFFEDnd 90  human
NP_001025810  35 LDPRTRRSYVRGRfLGKGGFARCYELTEAesREVYAGKVVPKSLLaKPHQKEKMSMEIAIHRSLaHRHVVGFQSFFEDgd 114 chicken
P70032        36 VDPRTRRRYLRGRfLGKGGFAKCYEITDLesREVFAGKIVPKTMLlKPHQKDKMTMEIAIQRSLdHRHVVGFHGFFEDnd 115 African clawed ...
Feature 1              ###  #                                           #  #          #          
2V5Q_A        95 FVFVVLELCRrRSLLELHKRRkaLTEPEARYYLRQIVLGCQYLHRNRVIHRDLKLGNLFLNedLEVKIGDFGLATKVEYD 174 human
2OU7_A       115 FVFVVLELCRrRSLLELHKRRkaLTEPEARYYLRQIVLGCQYLHRNRVIHRDLKLGNLFLNedLEVKIGDFGLATKVEYD 194 human
3D5W_A       116 FVYVVLEICRrRSLLELHKRRkaVTEPEARYFMRQTIQGVQYLHNNRVIHRDLKLGNLFLNddMDVKIGDFGLATKIEFD 195 zebrafish
3KB7_A        91 FVFVVLELCRrRSLLELHKRRkaLTEPEARYYLRQIVLGCQYLHRNRVIHRDLKLGNLFLNedLEVKIGDFGLATKVEYD 170 human
NP_001025810 115 FVYVVLELCRrRSLLELHKRRkaLSEPEVRYYLRQIILGCQYLHSQRVIHRDLKLGNLFLSddMEVKIGDFGLATKVEYD 194 chicken
P70032       116 FVYVVLELCRrRSLLELHKRRkaVTEPEARYYLKQTISGCQYLHSNRVIHRDLKLGNLFLNdeMEVKIGDFGLATKVEYD 195 African clawed ...
Feature 1                                                                                        
2V5Q_A       175 GERKKTLCGTPNYIAPEVLSkkGHSFEVDVWSIGCIMYTLLVGKPPFETsclKETYLRIKKNEYSIPKHINPVAASLIQK 254 human
2OU7_A       195 GERKKVLCGTPNYIAPEVLSkkGHSFEVDVWSIGCIMYTLLVGKPPFETsclKETYLRIKKNEYSIPKHINPVAASLIQK 274 human
3D5W_A       196 GERKKXLCGTPNYIAPEVLCkkGHSFEVDIWSLGCILYTLLVGKPPFETsclKETYIRIKKNEYSVPRHINPVASALIRR 275 zebrafish
3KB7_A       171 GERKKTLCGTPNYIAPEVLSkkGHSFEVDVWSIGCIMYTLLVGKPPFETsclKETYLRIKKNEYSIPKHINPVAASLIQK 250 human
NP_001025810 195 GEPKKTLCGTPNYIAPEVLGkkGHSFEVDIWSIGCIMYTLLVGKPPFETsclKETYIRIKKNEYTIPKHINPVAANLIQK 274 chicken
P70032       196 GERKKTLCGTPNYIAPEVLGkkGHSFEVDIWSIGCIMYTLLVGKPPFETsclKETYMRIKKNEYSIPKHINPVAAALIQK 275 African clawed ...
Feature 1                                 
2V5Q_A       255 MLQTdPTARPTINELLNDEFFTSGY 279 human
2OU7_A       275 MLQTdPTARPTINELLNDEFFTSGY 299 human
3D5W_A       276 MLHAdPTLRPSVAELLTDEFFTSGY 300 zebrafish
3KB7_A       251 MLQTdPTARPTINELLNDEFFTSGY 275 human
NP_001025810 275 MLRSdPATRPAINELLNDEFFTSGY 299 chicken
P70032       276 MLRSdPTSRPTIDDLLNDEFFTSGY 300 African clawed frog

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