3CW9,3CW8,1T5D


Conserved Protein Domain Family
CBAL
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cd05923: CBAL 
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4-Chlorobenzoate-CoA ligase (CBAL)
CBAL catalyzes the conversion of 4-chlorobenzoate (4-CB) to 4-chlorobenzoyl-coenzyme A (4-CB-CoA) by the two-step adenylation and thioester-forming reactions. 4-Chlorobenzoate (4-CBA) is an environmental pollutant derived from microbial breakdown of aromatic pollutants, such as polychlorinated biphenyls (PCBs), DDT, and certain herbicides. The 4-CBA degrading pathway converts 4-CBA to the metabolite 4-hydroxybezoate (4-HBA), allowing some soil-dwelling microbes to utilize 4-CBA as an alternate carbon source. This pathway consists of three chemical steps catalyzed by 4-CBA-CoA ligase, 4-CBA-CoA dehalogenase, and 4HBA-CoA thioesterase in sequential reactions.
Links
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Statistics
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PSSM-Id: 341247
Aligned: 7 rows
Threshold Bit Score: 742.018
Created: 22-Jun-2007
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 39 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:3CW9; Alcaligenes sp 4-Chlorobenzoyl-CoA ligase binds 4-chlorophenacyl-CoA and AMP, contacts at 4A.
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                                     
3CW9_A      2 QTVNEMLRRAATRAPDHCALAVPARGL-RLTHAELRARVEAVAARLhadglrPQQRVAVVAPNSADVVIAILALHRLGAV 80  Alcaligenes sp.
3CW8_X      2 QTVNEMLRRAATRAPDHCALAVPARGL-RLTHAELRARVEAVAARLhadglrPQQRVAVVAPNSADVVIAILALHRLGAV 80  Alcaligenes sp.
1T5D_X      2 QTVNEMLRRAATRAPDHCALAVPARGL-RLTHAELRARVEAVAARLhadglrPQQRVAVVAPNSADVVIAILALHRLGAV 80  Alcaligenes sp. AL...
BAB40576    2 RTAFELVAWSAHRQPGAVALLDPESGH-RLTYSELIKRIEGVAAVLasrgvvRDELVATAMANTLDHAIILLALNRLGAI 80  Arthrobacter sp. FHP1
ABI15621    2 QTVFDMVRRASARAPDAIAIVDPRRQI-EISYSQLLRQVERVAAGLvergvhPGSRVAVALPNSVEACILLLALHRAGAV 80  consortium cosmid ...
B42560      2 QTVHEMLRRAVSRVPHRWAIVDAARSTfDICRTGETSRNEGSATARlw--pqPARPLAVVSGNSVEAVIAVLALHRLQAV 79  Pseudomonas sp.
NP_770176   4 PMLDLASSFMASAARDPNAIALVDGDL-RLTYRQWYDKISALVASFdrlglkPGDHVVTLLQNRWEAATLHWACQIAGLV 82  Bradyrhizobium dia...

Feature 1           #                                                                         
3CW9_A     81 PALLNPRLKSAELAELIKRGEMTAAVIAVG---RQVADAIFQSgsgARIIFLGDLv---RDGEPYSYGPPIEDPq----r 150 Alcaligenes sp.
3CW8_X     81 PALLNPRLKSAELAELIKRGEMTAAVIAVG---RQVADAIFQSgsgARIIFLGDLv---RDGEPYSYGPPIEDPq----r 150 Alcaligenes sp.
1T5D_X     81 PALLNPRLKSAELAELIKRGEMTAAVIAVG---RQVADAIFQSgsgARIIFLGDLv---RDGEPYSYGPPIEDPq----r 150 Alcaligenes sp. AL...
BAB40576   81 PVIINPRLKAEEMVQLIRRDNIRSVIRTVD---KLGTPADIDG---VEELALSAEvlsgDLRINCNATPAFEAP------ 148 Arthrobacter sp. FHP1
ABI15621   81 PALMNPRLKPQEIRDLIEFGEMHGAVLCPAladAPPMREINGLf--LRVETEGQRd---GLGSLESDGPLPAFRp----- 150 consortium cosmid ...
B42560     80 PALMNPRLKPAEISELVARGEMARAVVAND---AGVMEAIRTRvpsVCVLALDDLv--sGSRVPEVAGKSLPPPp----c 150 Pseudomonas sp.
NP_770176  83 ITPINWRAKADELDYCIENAEACAVFHQDIs--AEPVQGSALA---GRLLRVSVDp---GTGEATSFSDLIKDRapdaep 154 Bradyrhizobium dia...

Feature 1               #                      #                  ##  ###                     
3CW9_A    151 EPAQPAFIFYTSGTTGLPKAAIIPQRAAESRVLFMSTQVGLRHGRHNVVLGLMPLYHVVGFFAVLVAALALDGTYVVVEE 230 Alcaligenes sp.
3CW8_X    151 EPAQPAFIFYTSGTTGLPKAAIIPQRAAESRVLFMSTQVGLRHGRHNVVLGLMPLYHVVGFFAVLVAALALDGTYVVIEE 230 Alcaligenes sp.
1T5D_X    151 EPAQPAFIFYTSGTTGLPKAAIIPQRAAESRVLFMSTQVGLRHGRHNVVLGLMPLYHVVGFFAVLVAALALDGTYVVVEE 230 Alcaligenes sp. AL...
BAB40576  149 RPEDPAFVFYTSGTTGLPKGVVIPHRAIEPRVLFMSTQAGLRFGDHNNLLGLMPIHHVIGFFGVFLGSLAFNGTWIPVTA 228 Arthrobacter sp. FHP1
ABI15621  151 GPEETAFVFYTSGTTGMPKGVVLSQRTAESRALFMVTQSGYRFGAHNRVIGLMPLYHVIGFFAVFVLALALNGRYYVVRD 230 consortium cosmid ...
B42560    151 EPEQAGFVFYTSGTTGLPKGAVIPQRAAESRVLFMATQAGLRHGSHNVVLGLMPLYHTIGFFAVLVAAMAFDGTYVVVEE 230 Pseudomonas sp.
NP_770176 155 RVGADAWSIMLYTSGTTSRPKGVPRRHRAERAAAIAHVAQNLYGRGERTLGVMPLYHTMGVRSLLAMSLIGGTFICLPRY 234 Bradyrhizobium dia...

Feature 1     #                   # ##                         ####                   ####### 
3CW9_A    231 FRPvdALQLVQQEQVTSLFATPTHLDALaaaaahagsslKLDSLRHVTFAGATMPDAVLETVHqh-lpGEKVNIYGTTEA 309 Alcaligenes sp.
3CW8_X    231 FRPvdALQLVQQEQVTSLFATPTHLDALaaaaahagsslKLDSLRHVTFAGATMPDAVLETVHqh-lpGEKVNIYGTTEA 309 Alcaligenes sp.
1T5D_X    231 FRPvdALQLVQQEQVTSLFATPTHLDALaaaaahagsslKLDSLRHVTFAGATMPDAVLETVHqh-lpGEKVNIYGTTEA 309 Alcaligenes sp. AL...
BAB40576  229 FDPaqAVKWIEELDVTCLFASPTHFDALlatse--fapeKLKSVDSLIFAGAAMNQSILKRLEnc-lqIPIVDIYGTTET 305 Arthrobacter sp. FHP1
ABI15621  231 FKPaeTAELIEAHRITGLFGTPTHLDALigsil--atprDLSSLETVTFAGATMPDPVLDRVNah-lpGRKTNIYGTTEA 307 consortium cosmid ...
B42560    231 FDAgnVLKLIERERVTAMFATPTHLDALttave--qagaRLESLEHVTFAGATMPDTVLERVNrf-ipGEKVNIYGTTEA 307 Pseudomonas sp.
NP_770176 235 DSRq-ALALIEKEEITNLYLVPTLYHDLvhhet--fagtNVSSVRKLGFAGASMTDGLLKKLNeafkpNLFVNHYGSSEI 311 Bradyrhizobium dia...

Feature 1     ##              #                                                               
3CW9_A    310 MNSLYMRQpk--tGTEMAPGFFSEVRIVrigggvd-eivangeEGELIVAa-sDSAFVGYLNQPQATaek-----lqDGW 380 Alcaligenes sp.
3CW8_X    310 MNSLYMRQpk--tGTEMAPGFFSEVRIVrigggvd-eivangeEGELIVAa-sDSAFVGYLNQPEATaek-----lqDGW 380 Alcaligenes sp.
1T5D_X    310 MNSLYMRQpk--tGTEMAPGFFSEVRIVrigggvd-eivangeEGELIVAa-sDSAFVGYLNQPQATaek-----lqDGW 380 Alcaligenes sp. AL...
BAB40576  306 MNSLFNPDat--qESGLRPGYHSRVQFAsvlesps-valpagvEGELVVDasaDATFTHYLNNQEATaak-----ivDGW 377 Arthrobacter sp. FHP1
ABI15621  308 MNSLFAEDpr--eGARLRPGFYSEVRVVpiggspq-halppgeEGELVVAadaDATFTEYLNQPDATrkk-----lhDGW 379 consortium cosmid ...
B42560    308 MNSLYMRAvr-iaGTVMRPGFFSEVRIVrvgg--------dvdDGCPTVKr-aSWRWRRRMRPFQATltnlrllqksFRK 377 Pseudomonas sp.
NP_770176 312 YTFTIDQNaaakpGSAGKAGLNQHVKVVrigarsvaelaavgeEGEIIATlagDEAFEGYWRRPEADaks-----lrEGW 386 Bradyrhizobium dia...

Feature 1       # #               #     ######                            #                   
3CW9_A    381 YRTSDVAVW-TPEGTVRILGRVDDMIISGGENIHPSEIERVLGTAPGVTEVVVIGLADQRWGQSVTACVVPrlgetlSAD 459 Alcaligenes sp.
3CW8_X    381 YRTSDVAVW-TPEGTVRILGRVDDMIISGGENIHPSEIERVLGTAPGVTEVVVIGLADQRWGQSVTACVVPrlgetlSAD 459 Alcaligenes sp.
1T5D_X    381 YRTSDVAVW-TPEGTVRILGRVDDMIISGGENIHPSEIERVLGTAPGVTEVVVIGLADQRWGQSVTACVVPrlgetlSAD 459 Alcaligenes sp. AL...
BAB40576  378 YRTGDSGYV-DDSGRVILTGRIDDMINTGAENVHAEEVEQIISRHPAVVEAAVVGLPDTRWGEVVTAVVVVse--plTAD 454 Arthrobacter sp. FHP1
ABI15621  380 YRTSDVAVM-HANGDVEIRGRLDDMIISGGENIHPSEVERVLRRHPSVREAVVLGVPDERWGQRVLACIVAhea-rpTAN 457 consortium cosmid ...
B42560    378 AGTGRAICVrDGSGNIVVLGRVDDMIISGGENIHPSEVERILAAAPGVAEVVVIGVKDERWGQSVVACVVLqpgasaSAE 457 Pseudomonas sp.
NP_770176 387 YFTGDTGYV-DPDGDLFVTGRVDDMIITGGENVSPVEIESCLSLHPDVDEVAVVGVADEKWGKIVAAFVKRnr--nvSES 463 Bradyrhizobium dia...

Feature 1                  # # #                     
3CW9_A    460 ALDTFCRSSELADFKRPKRYFILDQLPKNALNKVLRRQL 498 Alcaligenes sp.
3CW8_X    460 ALDTFCRSSELADFKRPKRYFILDQLPKNALNKVLRRQL 498 Alcaligenes sp.
1T5D_X    460 ALDTFCRSSELADFKRPKRYFILDQLPKNALNKVLRRQL 498 Alcaligenes sp. AL3007
BAB40576  455 LLDQVCLDSELANFKRPRRYFVVNELPRNAAMKVSRRTL 493 Arthrobacter sp. FHP1
ABI15621  458 ELDEFCIASELASFKRPRAYAFLDEIPRNAMNKVLVKAL 496 consortium cosmid clone pGZ1
B42560    458 RLDAFCRASALADFKRPRRYVFLDELPKSAMNKVLRRQL 496 Pseudomonas sp.
NP_770176 464 ELEQFCRTSGLANFKRPRRYVFVDAIPKSPVGKLLRRLL 502 Bradyrhizobium diazoefficiens

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