1PSB,1MHO,1CFP,1MQ1


Conserved Protein Domain Family
S-100B

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cd05027: S-100B 
Click on image for an interactive view with Cn3D
S-100B: S-100B domain found in proteins similar to S100B. S100B is a calcium-binding protein belonging to a large S100 vertebrate-specific protein family within the EF-hand superfamily of calcium-binding proteins. Note that the S-100 hierarchy, to which this S-100B group belongs, contains only S-100 EF-hand domains, other EF-hands have been modeled separately. S100B is most abundant in glial cells of the central nervous system, predominately in astrocytes. S100B is involved in signal transduction via the inhibition of protein phoshorylation, regulation of enzyme activity and by affecting the calcium homeostasis. Upon calcium binding the S100B homodimer changes conformation to expose a hydrophobic cleft, which represents the interaction site of S100B with its more than 20 known target proteins. These target proteins include several cellular architecture proteins such as tubulin and GFAP; S100B can inhibit polymerization of these oligomeric molecules. Furthermore, S100B inhibits the phosphorylation of multiple kinase substrates including the Alzheimer protein tau and neuromodulin (GAP-43) through a calcium-sensitive interaction with the protein substrates.
Statistics
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PSSM-Id: 240154
Aligned: 8 rows
Threshold Bit Score: 138.065
Created: 29-Dec-2006
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 10 residues -Click on image for an interactive view with Cn3D
Feature 1:Ca2+ binding site [ion binding site]
Evidence:
  • Structure:1MHO, Bos taurus holo S100B bound with calcium ions, contacts at 3.5 A
  • Comment:the N-terminal EF hand is a S100 specific feature while the C-terminal canonical EF hand is similar to the EF hands in calmodulin, troponin C, and parvalbumin
  • Comment:the second binding site (C-terminal EF hand) has a greater affinity for Ca2+ than the first (N-terminal EF hand)
  • Citation:PMID 3722173

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                      #  # #  #    #                             # # # #    #        
1PSB_A      1 SELEKAVVALIDVFHQYSGREGDKHKLKKSELKELINNELSHFLEEIKEQEVVDKVMETLDSDGDGECDFQEFMAFVAMI 80  cattle
1MHO        1 SELEKAVVALIDVFHQYSGREGDKHKLKKSELKELINNELSHFLEEIKEQEVVDKVMETLDSDGDGECDFQEFMAFVAMI 80  Bos taurus
1CFP_A      2 SELEKAVVALIDVFHQYSGREGDKHKLKKSELKELINNELSHFLEEIKEQEVVDKVMETLDSDGDGECDFQEFMAFVAMI 81  cow
1MQ1_A      1 SELEKAMVALIDVFHQYSGREGDKHKLKKSELKELINNELSHFLEEIKEQEVVDKVMETLDNDGDGECDFQEFMAFVAMV 80  human
XP_421894   2 SELEKAMIAIIDAFHQYSGKEGDKHKLKKSELKELINNELTHFLGEIKDQETVDKVMEALDSDGDAECDFQEFVAFIAMV 81  chicken
XP_548737 366 SQLEKAALALLDVFQQYSARAGGGRRLRKADVKELIGSELAHFLEEIKEQEVVDKVMETLDSDGDGECDFQEFVAFVAMV 445 dog
AAH76251    2 SDLENCLGTIIEVFHKYSSKEGDKYKLKKSELKELLTHEFPTLTEQVKDQATMDSLMESLDTDGDSECDFQEFMTFVTMV 81  zebrafish
BAE36647    2 SELEKAMVALIDVFHQYSGREGDKHKLKKSELKELINNELSHFLEEIKEQEVVDKVMETLDEDGDGECDFQEFMAFVAMV 81  house mouse
Feature 1             
1PSB_A     81 TTACHEFF 88  cattle
1MHO       81 TTACHEFF 88  Bos taurus
1CFP_A     82 TTACHEFF 89  cow
1MQ1_A     81 TTACHEFF 88  human
XP_421894  82 TSACHEFF 89  chicken
XP_548737 446 TTACHEFF 453 dog
AAH76251   82 TICCHEFF 89  zebrafish
BAE36647   82 TTACHEFF 89  house mouse

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