2CRW,2P57,2OWA,5NZS


Conserved Protein Domain Family
ArfGap_ArfGap2_3_like

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cd08831: ArfGap_ArfGap2_3_like 
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Arf1 GTPase-activating protein 2/3-like
ArfGAP (ADP Ribosylation Factor GTPase Activating Protein) domain is a part of ArfGap1-like proteins that play a crucial role in controlling of membrane trafficking, particularly in the formation of COPI (coat protein complex I)-coated vesicles on Golgi membranes. The ArfGAP1 protein subfamily consists of three members: ArfGAP1 (Gcs1p in yeast), ArfGAP2 and ArfGAP3 (both are homologs of yeast Glo3p). ArfGAP2/3 are closely related, but with little similarity to ArfGAP1, except the catalytic ArfGAP domain. They promote hydrolysis of GTP bound to the small G protein ADP-ribosylation factor 1 (Arf1), which leads to the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. Thus, the GAP catalytic activity plays a key role in the formation of COPI vesicles from Golgi membrane. In contrast to ArfGAP1, which displays membrane curvature-dependent ArfGAP activity, ArfGAP2 and ArfGAP3 activities are dependent on coatomer (the core COPI complex) which required for efficient recruitment of ArfGAP2 and ArfGAP3 to the Golgi membrane. Accordingly, ArfGAP2/3 has been implicated in coatomer-mediated protein transport between the Golgi complex and the endoplasmic reticulum. Unlike ArfGAP1, which is controlled by membrane curvature through its amphipathic lipid packing sensor (ALPS) motifs, ArfGAP2/3 do not possess ALPS motif.
Statistics
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PSSM-Id: 350060
Aligned: 101 rows
Threshold Bit Score: 145.767
Created: 20-Jan-2010
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Zn binding sitearginine finger
Conserved site includes 4 residues -Click on image for an interactive view with Cn3D
Feature 1:Zn binding site [ion binding site]
Evidence:
  • Structure:2P57; human GAP domain of ArfGAP2 binds Zn(2+) ion
  • Comment:The coordination of the Zn2+ ion by 4 cysteines would indicate that this ion plays a structure stabilization rather than catalytical role.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                          #  #                  #  #                                    
2CRW_A        15 DILTIFKRLRSVp-tNKVCFDCGA--KNPSWASITYGVFLCIDCSGSHRSLGVHLSFIRSTELDsnWSWFQLRCMQVGGN 91  human
2P57_A        23 EIQTLFKRLRAVp-tNKACFDCGA--KNPSWASITYGVFLCIDCSGVHRSLGVHLSFIRSTELDsnWNWFQLRCMQVGGN 99  human
XP_001308480   3 DNKDELRRLARRp-eNKTCADCGA--KNPTWASVTYGIWICLECAGKHRGLGVHVSFVRSLDLDs-WTDEQINVMKCGGN 78  Trichomonas vag...
XP_002677017   4 ENQRILKKLREKp-eNKVCIDCTT--KNPDWCSITFGVFFCLSCSGVHRSLGVDVSFVKSATLDk-WSDEHTQAMVNGGN 79  Naegleria grube...
XP_003958284  14 KVQEVFDKLRSQdsrSRFCFDCGA--EDAAWVSVPFAVMLCLSCSNIHRTMDVGITYVKSSIIDq-WTVNNLKGFKYGGN 90  Kazachstania af...
ELA42911       5 DYKKLVGNKSKLs-eNSFCVDCGT--KSPKWASVRYGTFFCLDCAAVHRSLGVYLDFVKSVNLDg-WDKESYLPMEYGGN 80  Vittaforma corn...
CAD25965       4 NFNKEVKALREIe-eNKKCADCSA--PSPPWTSVTYGIFICFDCASVHRSLGVKTSFVKSVNLDi-WDEKEYLFMKHGSN 79  Encephalitozoon...
XP_002995427   6 SYNSQLVELLKNp-sNNRCIDCNI--PNTQWTSKTFGIFLCFDCTSIHRSFGVNISFVKSVNMDk-WSQVEYLFMKLGGN 81  Nosema ceranae ...
EOB14628       4 LCNDKVKKLSKEp-tNNKCMDCNV--SNPQWASVTFGVFICLDCASVHRSMGVNTSFVKSVTMDs-WSQKEYLFMKLGSN 79  Nosema bombycis...
EAX95704       2 DAKPFLSELQRQp-eNKVCFECGKrnPNPDWASVTYGVWICVECAGKHRSLGTHMSFVQSLSLDk-WTEANIAKMKVGGN 79  Trichomonas vag...
Feature 1                                                  
2CRW_A        92 ASASSFFHQhgc---stNDTNAKYNSrAAQLYREKIKSLASQ 130 human
2P57_A       100 ANATAFFRQhgc---taNDANTKYNSrAAQMYREKIRQLGSA 138 human
XP_001308480  79 KKARDYFKSigi---daLSVSAKYKSrGAKQYAAQLYAEAGA 117 Trichomonas vaginalis G3
XP_002677017  80 KKAREYFTSkgi---drMPIAQKYNSkAAKEYAALLKQQVQQ 118 Naegleria gruberi strain NEG-M
XP_003958284  91 QKAYDYFTLhsadkllqASIKEKYTNeIAAKYRHHIDSLVKR 132 Kazachstania africana CBS 2517
ELA42911      81 KRFVDYVEMkgl---kdLDIESKYKSsEIIEYSKELMKMIHK 119 Vittaforma corneae ATCC 50505
CAD25965      80 EKFRRFLEQhrl---vgREMNEIYNNnQIKRYAAEIKGLVVK 118 Encephalitozoon cuniculi GB-M1
XP_002995427  82 EKFTEFLEKhnl---qnKECNVLYYDpLVKKYGEKLKSRVYE 120 Nosema ceranae BRL01
EOB14628      80 EAFQGFINQqgl---knRDTLEIYVNnGAKKYSNKLKERVFK 118 Nosema bombycis CQ1
EAX95704      80 SKAMKYFQSrgi---dkLEIHQKYSNsFAKQYAQQITADSNK 118 Trichomonas vaginalis G3

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