1Z57,2VAG


Conserved Protein Domain Family
PKc_CLK1_4

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cd14213: PKc_CLK1_4 
Click on image for an interactive view with Cn3D
Catalytic domain of the Dual-specificity protein kinases, CDC-like kinases 1 and 4
Dual-specificity PKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine as well as tyrosine residues on protein substrates. CLK1 plays a role in neuronal differentiation. CLKs are involved in the phosphorylation and regulation of serine/arginine-rich (SR) proteins, which play a crucial role in pre-mRNA splicing by directing splice site selection. SR proteins are phosphorylated first by SR protein kinases (SRPKs) at the N-terminus, which leads to its assembly into nuclear speckles where splicing factors are stored. CLKs phosphorylate the C-terminal part of SR proteins, causing the nuclear speckles to dissolve and splicing factors to be recruited at sites of active transcription. Based on a conserved "EHLAMMERILG" signature motif which may be crucial for substrate specificity, CLKs are also referred to as LAMMER kinases. CLKs autophosphorylate at tyrosine residues and phosphorylate their substrates exclusively on serine/threonine residues. The CLK1/4 subfamily is part of a larger superfamily that includes the catalytic domains of other protein serine/threonine PKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase.
Statistics
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PSSM-Id: 271115
Aligned: 6 rows
Threshold Bit Score: 626.878
Created: 24-Dec-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 19 residues -Click on image for an interactive view with Cn3D
Feature 1:ATP binding site [chemical binding site]
Evidence:
  • Structure:1Z57; Human CLK1 binds 10z-Hymenialdisine; contacts at 4A
  • Structure:2VAG; Human CLK1 binds indole inhibitor; contacts at 4A
  • Comment:also based on the structures of other CMGC kinases bound with ATP or ATP analogs
  • Citation:PMID 9739089

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                           ####    #             # #                                 #  
1Z57_A         3 HLICQSGDVLSaRYEIVDTLGEGAFGKVVECIDHkaggRHVAVKIVKNvdrYCEAARSEIQVLEHLNTTDpnsTFRCVQM 82  human
2VAG_A         3 HLICQSGDVLSaRYEIVDTLGEGAFGKVVECIDHkaggRHVAVKIVKNvdrYCEAARSEIQVLEHLNTTDpnsTFRCVQM 82  human
XP_414614    164 HLICESGDVLRaRYEIVATLGEGAFGKVVECIDHdmrgMHVAVKIVKNvgrYREAARSEIQVLEHLNTMDpssTFRCVQM 243 chicken
Q9HAZ1       146 HLICQSGDVLRaRYEIVDTLGEGAFGKVVECIDHgmdgMHVAVKIVKNvgrYREAARSEIQVLEHLNSTDpnsVFRCVQM 225 human
NP_001091432 128 HLICESGDVLReRYEIVHNVGEGAFGKVVECIDLtgsgKRVAVKIIKNverYREAAQSEIKVLEHLSRKDpknTFRCVQM 207 African clawed ...
NP_001133527 157 HLIYHRRDMLRaRYEIVSTLGEGAFGKVVECIDHskigSRVALKIIKNidrYREAAMAEVEVLEQMNSLDcdrRYGCVRM 236 Atlantic salmon
Feature 1                     ####  #  #                                         ## #            
1Z57_A        83 LEWFEHHGHICIVFELLGLSTYDFIKENgfLPFRLDHIRKMAYQICKSVNFLHSNKLTHTDLKPENILFVQSDYTEAYNP 162 human
2VAG_A        83 LEWFEHHGHICIVFELLGLSTYDFIKENgfLPFRLDHIRKMAYQICKSVNFLHSNKLTHTDLKPENILFVQSDYTEAYNP 162 human
XP_414614    244 LEWFDHHGHVCIVFELLGLSTYDFIKENsfLPFHINDIRNMAYQICQSINFLHHNKLTHTDLKPENILFVESDYIVKYNA 323 chicken
Q9HAZ1       226 LEWFDHHGHVCIVFELLGLSTYDFIKENsfLPFQIDHIRQMAYQICQSINFLHHNKLTHTDLKPENILFVKSDYVVKYNS 305 human
NP_001091432 208 LEWFDHHGHVCIVFELLGLSTYDFIKENsfLPFPMDQIRNMAYQICKSINFLHQNQLTHTDLKPENILFVHSDYVVNFNP 287 African clawed ...
NP_001133527 237 LDWFDHHGHVCISFELLGLSTYDYLKENnfQPFPIEHIRIMAYQIIRAVRFLHKNKLTHTDLKPENILFVDSEYDIEYNA 316 Atlantic salmon
Feature 1                        ##                                                              
1Z57_A       163 KIKRDERTLiNPDIKVVDFGSATYDDEHHSTLVSTRHYRAPEVILAlGWSQPCDVWSIGCILIEYYLgFTVFPTHdSKEH 242 human
2VAG_A       163 KIKRDERTLiNPDIKVVDFGSATYDDEHHSTLVXXRHYRAPEVILAlGWSQPCDVWSIGCILIEYYLgFTVFPTHdSKEH 242 human
XP_414614    324 KMKRDERTLkNTDIKVVDFGSATFDDEHHSTLVSTRHYRAPEVILAlGWSQPCDVWSIGCILIEYYLgFTVFQTHdSKEH 403 chicken
Q9HAZ1       306 KMKRDERTLkNTDIKVVDFGSATYDDEHHSTLVSTRHYRAPEVILAlGWSQPCDVWSIGCILIEYYLgFTVFQTHdSKEH 385 human
NP_001091432 288 TKKRDERYLkNSDIKIVDFGSATYNHEYHSALVSTRHYRAPEVILElGWSQPCDVWSIGCILIEYYLgLTVFQTHdSKEH 367 African clawed ...
NP_001133527 317 KMKRDERTLkNPDVKVVDFGNATYEHNHHTSVVSTRHYRAPEVILDlGWDHACDVWSLGCILIEYYLgSTLFQTHdSKEH 396 Atlantic salmon
Feature 1                                                                                        
1Z57_A       243 LAMMERILGPlPKHMIQKTrKRKYFHHDRLDWDEHSSAGryvsRACKPLKEFMLSQDVEHERLFDLIQKMLEYDPAKRIT 322 human
2VAG_A       243 LAMMERILGPlPKHMIQKTrKRKYFHHDRLDWDEHSSAGryvsRACKPLKEFMLSQDVEHERLFDLIQKMLEYDPAKRIT 322 human
XP_414614    404 LAMMERILGPlPAHMIKKS-RKHYFHHNQLDWDEHSSAGryvrRRCKPLKEFMHCQDTDHQSLFDLVRRMLEYDPAKRIT 482 chicken
Q9HAZ1       386 LAMMERILGPiPQHMIQKTrKRKYFHHNQLDWDEHSSAGryvrRRCKPLKEFMLCHDEEHEKLFDLVRRMLEYDPTQRIT 465 human
NP_001091432 368 LAMMERILGPlPLHMIQKTrKHKYFYHDRLDWDEHSSAGryvkKRCKPLKEYMVSDNKDHHQLFDLIRRMLEYDPAKRIT 447 African clawed ...
NP_001133527 397 LAMMERVLGPiPVHLLQKTkKRRYVHRYQLDWDALSSSGryvrKHCKPLKQYMMVQSLDHDQLFDLIQKMLEYDPAKRLS 476 Atlantic salmon
Feature 1                  
1Z57_A       323 lREALKHPFF 332 human
2VAG_A       323 lREALKHPFF 332 human
XP_414614    483 lDEALQHPFF 492 chicken
Q9HAZ1       466 lDEALQHPFF 475 human
NP_001091432 448 lEEAMKHPFF 457 African clawed frog
NP_001133527 477 lDQALRHPFF 486 Atlantic salmon

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