2A8Y,3OZB,1CG6,5EUB,5F73,1WTA,1V4N


Conserved Protein Domain Family
MTAP_SsMTAPII_like_MTIP

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cd09010: MTAP_SsMTAPII_like_MTIP 
Click on image for an interactive view with Cn3D
5'-deoxy-5'-methylthioadenosine phosphorylases (MTAP) similar to Sulfolobus solfataricus MTAPII and Pseudomonas aeruginosa PAO1 5'-methylthioinosine phosphorylase (MTIP)
MTAP catalyzes the reversible phosphorolysis of 5'-deoxy-5'-methylthioadenosine (MTA) to adenine and 5-methylthio-D-ribose-1-phosphate. This subfamily includes human MTAP which is highly specific for MTA, and Sulfolobus solfataricus MTAPII which accepts adenosine in addition to MTA. Two MTAPs have been isolated from S. solfataricus: SsMTAP1 and SsMTAPII, SsMTAP1 belongs to a different subfamily of the nucleoside phosphorylase-I (NP-I) family. This group also includes Pseudomonas aeruginosa PAO1 MTI phosphorylase (MTIP) which uses 5'-methylthioinosine (MTI) as a preferred substrate, and does not use MTA. NP-I family members accept a range of purine nucleosides as well as the pyrimidine nucleoside uridine. The NP-1 family includes phosphorolytic nucleosidases, such as purine nucleoside phosphorylase (PNPs, EC. 2.4.2.1), uridine phosphorylase (UP, EC 2.4.2.3), and 5'-deoxy-5'-methylthioadenosine phosphorylase (MTAP, EC 2.4.2.28), and hydrolytic nucleosidases, such as AMP nucleosidase (AMN, EC 3.2.2.4), and 5'-methylthioadenosine/S-adenosylhomocysteine (MTA/SAH) nucleosidase (MTAN, EC 3.2.2.16). The NP-I family is distinct from nucleoside phosphorylase-II, which belongs to a different structural family.
Statistics
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PSSM-Id: 350161
Aligned: 545 rows
Threshold Bit Score: 175.301
Created: 23-Jul-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 20 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:2A8Y: Sulfolobus solfataricus MTAP homohexamer bound with 5'-deoxy- 5'methylthioadenosine and sulfate in each of 6 active sites; contacts at 4A
  • Comment:unique dimer-of-trimers quaternary structure
  • Comment:the neighboring subunit contributes residue(s) to the protein-ligand interaction

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1          #                                            ##             #              
2A8Y_A     10 IGIIGGsGLYDPGifsesKEIKVYTpygqpsDFITIGKig--nKSVAFLPRHg------rGHRIPPHKINYRANIWALKE 81  Sulfolobus solfata...
OLS17857    9 IGVIGGsGFYHLLda--aELKTIETpfg-stPPLSIGYik--sTKVVFLPRHakpggdkiTHALPPHMINYKANVYALYK 83  archaeon Odin LCB_4
ODA43343    3 IGIIGGsGLSKSKie--lKSFTLETpyg-kpSSAYELQei-dnKNIFFLRRHg------eGHTIPPHKVNYRANIFGFKE 72  Thermodesulfovibri...
ESJ99263    4 IGIIGGsGLENITslkfyGEEFIETpygrpsCKYKIYKns--dVIVYSLSRHg------dDHRFAPHQINYKANIYGFYQ 75  Mucispirillum scha...
ADD67275    4 LGIIGGsGLYDIEgfeyvEEIIMQTeygdpsDTYRVFSyk--dMRFYFLNRHg------rSHSVPPHKVNYRANIDGFSR 75  Denitrovibrio acet...
BAI79968    3 IGIIGGsGLYEIDglefiEEIKLDNrfgspsDSYKVFDyk--dCKFYFLNRHg------kNHKILPHNVNYRANIYGFYE 74  Deferribacter desu...
OLS28318    2 ICVIGGsGFYNFLed--vENKIIETpf--geVSVEVGKiq--nKDVIFLPRHg------kRHSIAPDQINYRANIFTAHL 69  archaeon Heimdall ...
AEI15441    3 IGIIGGsGLYSIEafnytGEEKIDTdygepsAAFKIYEyy--gNRYYFLARHg------aGHEFPPHLVNYRANISAFKK 74  Flexistipes sinusa...
OLS28565    2 IGIIGGsGFYSFLen--pKVEKIQTp----fGEISIYLdqkdgKDILFIPRHg------sGHSILPSRINYRANIYAAHL 69  archaeon Heimdall ...
ADR18044    3 IGIISGtGFLVEEgfkfvEEINLSNkygspsSSYKKYLfg--dNEIYSIARHg------eKHQFPPHLINYRSNIYGFYE 74  Calditerrivibrio n...
Feature 1              ####                                                 #                 
2A8Y_A     82 LGVRWVISVSAVGSLRMdyKLGDFVIPDQFIDMTk-nREYSFFDgpv-------------vaHVSMADPFCNsLRKLAIE 147 Sulfolobus solfata...
OLS17857   84 LNVTRIIATHSCGSIRKeiKPGSLVIPDQFIDMTk-rRGSTFFNgelnskis--lkestgvvHIDVSKPFCSeLSEIIYE 160 archaeon Odin LCB_4
ODA43343   73 LKVERIFGVFAVGSLNEniPPGSIVIPNQIIDFTqgmRENTFFDgpk-------------vvHIDFTNPFCEeLRTLIFK 139 Thermodesulfovibri...
ESJ99263   76 AGVQEILSFSAVGGINLnyKNGDLVLTDDAIDNTv-rGNVTFYDrtg------------yvvHIDMSLPFCPsLRLKLKE 142 Mucispirillum scha...
ADD67275   76 LGIENIVSITATGGINSsyKPGDIIIPDDGIDMTs-gRIQTFYEskq-------------ihHIDFTEPFCPrLRGILEN 141 Denitrovibrio acet...
BAI79968   75 LGVKHIFSFTAVGGINKllKPADIVIPDNAIDMTy-sRNSTFYDele------------ekyHIDFTNPFCTnLRNILIK 141 Deferribacter desu...
OLS28318   70 LKTEMIFATNAVGSIRQsiSPGSFVTPDHIIDLTh-gRKSTFFDgtglslttikgkklsgvvHVDVSDLFDLkLRNLIKQ 148 archaeon Heimdall ...
AEI15441   75 AGVDAILSFAAVGGINAdlSPGDIVISDNAIDFTn-gRNHTFATkgn-------------ihHIDITEPFCPeLRKAVKK 140 Flexistipes sinusa...
OLS28565   70 KNVERIYATNAVGTSNQkiNPGVIVILDQIIDVTn-gRDTSFFTgddfsiitrsgrklsgvvHTDVSEPFDNnVRRDLLN 148 archaeon Heimdall ...
ADR18044   75 LNVDFIVAFGAVGGINSllLPGDFLIPDNIVDWTy-gRESTYSDigd-------------tyHIDFTYPFCPsIREELIN 140 Calditerrivibrio n...
Feature 1                      #    #                 ####                     ## #          #
2A8Y_A    148 TAkelnikTHESGTYICIEGPRFSTRAESRTWREVYKadIIGMTLVPEVNLACEAQmCYATIAMVTDYDVFaei--pvTA 225 Sulfolobus solfata...
OLS17857  161 VGvklgakPINRGVYVCTEGPRFETPAEIKFFEKIGGd-IVGMTLIPELVLARELGiCYSSISLVSNYAAGvtd-rkiTF 238 archaeon Odin LCB_4
ODA43343  140 SAekmgisLFTEGTYICINGPRLETSAEIKFYKNIGAd-IIGMTLMPEASLAREAEiCYVALTVVANYAAGisk-sklTV 217 Thermodesulfovibri...
ESJ99263  143 SAdlcgidLIDKGIYICTNGPRFETPAEIKMYRNWGAd-MVGMTLFPEVTLSKEMGiCYANISLVTNAAAGveenrklTS 221 Mucispirillum scha...
ADD67275  142 SAesadvdAAVNGTYVCTNGPRMETKGEIKAFDRWGAd-LVGMTLFPECSLARERElCYANLSVITNYAAGtss-tklTA 219 Denitrovibrio acet...
BAI79968  142 SCmaqnlrFFDVGTYICTNGPRLETAAEIKFYKQIDAd-IVGMTLFPEAPLARELEiCYANMSIVTNYAAGisk-tklTV 219 Deferribacter desu...
OLS28318  149 SGlklgmkILEQGTLVVTNGPRFESPAEIKAYGLWGGd-FVGMTSAPEAFLAKELGiPYATLAVITNYAAGlqs--nvTA 225 archaeon Heimdall ...
AEI15441  141 SCdssglnFSDKGVYICTNGPRMETAAEIKAFRGMGAd-IVGMTLFPECSLAREAEiCYVNISVVANDAAGisr-nklTA 218 Flexistipes sinusa...
OLS28565  149 ACkhynqeTVDGGTVAVFNGPRYETPAEVRAYTTLGAr-YFGMTSAPEAFLAKEIEiPYATLAVVTNYAAGlqs--tvTH 225 archaeon Heimdall ...
ADR18044  141 ILkksgvrFHPKGVYLCTNGPRLESAAEINAFLKLGFd-VVGMTLMPETALARELGiCYAAVNIVSNFAAGisk-tplTT 218 Calditerrivibrio n...
Feature 1       #                   
2A8Y_A    226 EEVTRVMAENTEKAKKLLYALI 247 Sulfolobus solfataricus
OLS17857  239 NEVVEEFEKNRDKLFKILREAV 260 archaeon Odin LCB_4
ODA43343  218 KEVVETMNEKVEHIRKLIKETL 239 Thermodesulfovibrio sp. N1
ESJ99263  222 DEVIEEAKRNTLKISKIIEQYL 243 Mucispirillum schaedleri ASF457
ADD67275  220 DEVVEEMGKATEKLKGIVSMLP 241 Denitrovibrio acetiphilus DSM 12809
BAI79968  220 DEVIDTMKKNNENIKKVIQKIP 241 Deferribacter desulfuricans SSM1
OLS28318  226 DEVFELFKSKINDVKSIIKKAI 247 archaeon Heimdall LC_2
AEI15441  219 DEVVETVKKSEDKLKRIILNVD 240 Flexistipes sinusarabici DSM 4947
OLS28565  226 DEVDSLFKNRITDVKNILNYAI 247 archaeon Heimdall LC_2
ADR18044  219 DEVVEHVKNNDAVIRFIIKEVA 240 Calditerrivibrio nitroreducens DSM 19672

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