Conserved Protein Domain Family
diphth2_R
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TIGR00322: diphth2_R 
diphthamide biosynthesis enzyme Dph1/Dph2 domain
Archaea and Eukaryotes, but not Eubacteria, share the property of having a covalently modified residue, 2'-[3-carboxamido-3-(trimethylammonio)propyl]histidine, as a part of a cytosolic protein. The modified His, termed diphthamide, is part of translation elongation factor EF-2 and is the site for ADP-ribosylation by diphtheria toxin. This model includes both Dph1 and Dph2 from Saccharomyces cerevisiae, although only Dph2 is found in the Archaea (see TIGR03682). Dph2 has been shown to act analogously to the radical SAM (rSAM) family (pfam04055), with 4Fe-4S-assisted cleavage of S-adenosylmethionine to create a free radical, but a different organic radical than in rSAM.
Links
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Statistics
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PSSM-Id: 273013
Aligned: 80 rows
Threshold Bit Score: 203.201
Created: 7-Oct-2014
Updated: 25-Oct-2021
Structure
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Aligned Rows:
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PubMed ReferencesClick to see Conserved Features Help
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
XP_001418709  10 VAYDVARTASWIRDGAFSRVALQLPDDKLPDAARLARalqtALardddAARASREVFVLADTTFGSCCVDEVAAAHRDAD 89  Ostreococcus lu...
XP_967520     31 EVYELQRCAGWIKANDFQKVCLQFPDHLLQDSSEVAL----RL-----GKQVGRTVYILADTAYESCCIDYVAAGHINAD 101 red flour beetle
XP_002432293  31 DKYNLTKCIEWINENNFKRTCLQFPNDLLNDGTSIAL----YL-----QKTFDRTFYILGDTSYGSCCVDEVASEHVNGD 101 human body louse
EFN72571       6 SAYDVEKCIQWIQTNALKQVCLQFPDSLLPDSVEVAL----HL-----EKSIEQKVYILGDTTCGSCCVDEIAAQHINAD 76  Florida carpent...
NP_001040357  29 TNFDIKETCKWIERKKFAKVCLQFPDDLLEVSCKVYK----EI-----KDRIEVDLYIMGDTSYASCSIDSVAAMHVQAN 99  domestic silkworm
XP_001949323   4 YSEEIFCCSQWIKNGKFEKVCIQLPDYMLCKSVSISS----EF-----ERLLAHRVYILGDTSYGSCCVDETAAQHIAAD 74  pea aphid
XP_309119     37 NAEQRDRCLRWIQKHSLKRVALQLPDSALHHSVQIVS----ELrd--vASDSELALFVLADTSYGSCCVDEIAAAHANAD 110 Anopheles gambi...
XP_001863593  32 NSDERDQLVQWIRTNDFMKVALQFPDVLLPFSAVVAN----EL-----QSASTAGIFILGDTSYGSCCVDEVAASHVGAD 102 southern house ...
XP_002097817  36 NENHRRSCVDWIRKSGYKRVCLQFPDDYLPHSNAISV----GLke--lLAPEDVKVFILADTTYGSCCVDEIAAAHVDAD 109 Drosophila yakuba
XP_001022303  30 QYYDIPTIIQEIKDNNYKDIILQFPVENLSDAIKVQS----SIn----KNLPATNIYIISDHLKNSCCVDIVGSNHINYD 101 Tetrahymena the...

XP_001418709  90 AIVHFGRACCSP--TSRTPARLVFDRKEIDCARCAR-ALEAHaaasaRRGARAVVALCDQEYFWALDE-LKRAStsstan 165 Ostreococcus lu...
XP_967520    102 AIIHFGPVCFSKt-SANIPYLNIYEKDELDLGALEQ-CL--------VPLNEKIVILLDTSFIHLTDK-IVAKF------ 164 red flour beetle
XP_002432293 102 SIIHFGHACLSP--VIKLPVFYIFPKENLNLDLFCR-CLDEDf----ECDHQEIIIFYDDSYHHLYEK-MKSTLkhkp-- 171 human body louse
EFN72571      77 GIIHFGHACLNP--TIRLPVLYILPKKEFDTTKLSN-EFKRVf----SDCSKKILFFYDVTYAHKIEH-VYDIL------ 142 Florida carpent...
NP_001040357 100 GIVHYGHKCFS---KTDIPVYMVLPKEKLDFDAAIN-TLKEKy---dSDDNMKLCLFYGAEFEHCNENiTRKWF------ 166 domestic silkworm
XP_001949323  75 CIIHFGRSCLTP--NSHLPVYYVLPKQPLDDVQCAN-SIIKTf----ETDEGLVLVLYDVAYHHVRDH-LAKSL------ 140 pea aphid
XP_309119    111 GIVHFGHACLSK--VARIPVLYVFLQLTFDEEHLLA-ALSQEl----PDCTVALRCFYEVAYANAVAR-LKGAL------ 176 Anopheles gambi...
XP_001863593 103 AVIHFGHACLSR--SVRLPTRYVFLRGPLDVGSVVQ-GVKEVl---gEHEGKRLGLFYDVAYDYCVGE-VAERL------ 169 southern house ...
XP_002097817 110 SVIHFGNACRSR--ATRLPVLYLYPELPLDVNVLLD-KLLSLr---aDSKDRQLCVYLDIGYQYLYGEqLKKQLle---- 179 Drosophila yakuba
XP_001022303 102 IIFHFGVACLSEseEQQKKIKYIFPKTHMDLVGFTEqAFKLTk---sDDGKVQNYIILIDQYQYYLSQeIFQQF------ 172 Tetrahymena the...

XP_001418709 166 vqgaeiFIADAVEVEVDPS--RDsrvRGRde-dASTTrvGASRFRPPNGtt------nEDCAYVWIGATGPA--MTHA-M 233 Ostreococcus lu...
XP_967520    165 ------PDHTIKTINSDNL--TIe----------------------------------NDTVV-FIGGRNHHrkLPNLkF 201 red flour beetle
XP_002432293 172 --nvfvSKLNLSTASCDSN--NDenrIIC----GRIF--KCQSn--------------ETTCAIFLGKECVT--LENL-V 224 human body louse
EFN72571     143 -------KPIYKHLILSKLncTSnvkYTDtnsaSIVL--GRNY-TLENGyn------iEDYEGFFLGEEGKT--FSVL-A 203 Florida carpent...
NP_001040357 167 ------QKYPKSYICHIELedKPerfL------GRIIkdVQNVEYPIQAw--------KDGICIYLGSRGQT--IFNY-T 223 domestic silkworm
XP_001949323 141 --------APAKSIILSDIdlKEqntTSCtvsdGQEY--TCIFGRQFPNl--------NYKNIVYIVHDNYMnnVDRF-I 201 pea aphid
XP_309119    177 ------LARYPSATVATIA--EP---DQQ----ADVL---CWKLDEGAHpp------nGERPVLYIGRDNLS--FFNT-A 229 Anopheles gambi...
XP_001863593 170 ------KEAGRN-VTVGQL--AK---TGE----QPDL--LCWKLPGGASf--------DDAVCIFIGHDNQS--FFNT-- 219 southern house ...
XP_002097817 180 -------------------------aLEP----KELL--LELFPPIEADskttkettsLERICIFIGADNQR--FANL-S 225 Drosophila yakuba
XP_001022303 173 ------RDQENVTLALIQL--DQaqkINH-------LfyGYYLNKSLDKs--------KKYQLIFTGHEDSD--LLEKiF 227 Tetrahymena the...

XP_001418709 234 LVlgdcAAKF---GgMAQYDPSvdgDAVRVEadGAGEAARALKRRRFLIAKAKEARVVGIIAGTLGVAGYREMIENLRKL 310 Ostreococcus lu...
XP_967520    202 SH----KPKT-----LYYFNDK---APSLIN--YTLDE-KVLRRRYFLVEKIKDSNTVGIVIGTLGVSNYLKAIERLKKL 266 red flour beetle
XP_002432293 225 LS----LPEF---D-WFLYNDE---SSNIIK--YQMNRNKFLMKRIHLVEKIKESKTIGILIGTLGIKNYLQAIDRIKKL 291 human body louse
EFN72571     204 MS----IPVK---R-WYSFA-----NSEIHE--FEALNTPWLKRRRFLIEKLKDAKVVGILVATLGIKEYLTAISTVKRI 268 Florida carpent...
NP_001040357 224 IS----IPAT---K-GYLLNPS---SSQL----EQWDETIWFKRRRFLIEKCKDANIMGILICKLSGKQTKDIISRMKEL 288 domestic silkworm
XP_001949323 202 LE----KKEC---Q-VYIYQNS---CNIL----TQRSLSSVIKKKNFTKEKIKDSTHIGILICSLSIKDLLERINRVKCL 266 pea aphid
XP_309119    230 VA----LETS---Q-CYLYDTS---RPDRQLvrTDAVGAKWLRRRYYAIERCMDARSIGIVVATLSTKGYLEIVSRVQRL 298 Anopheles gambi...
XP_001863593 220 ------ANGIraeQ-WFRFDPA---TSQLQQ--ANPLDSRWMRRRFYYIEKCKDATSLGIVVATLTAAGYLDVVNRIQRL 287 southern house ...
XP_002097817 226 LT----APAA---VqWHIYDGS---SGSLSS--KNPLTAQFIRRRYFHIEKCKDAQTLGLIVATLSAEGYLDVVTRLQTM 293 Drosophila yakuba
XP_001022303 228 FA----KDKFnieK-VYVY------NNQIKE--SPPVSNKSLIKRLSMIEQARDIKCFGILIQNPNTQFCRAVTERVKQV 294 Tetrahymena the...

XP_001418709 311 IANSGRKSYTVVAGKPNPQKLANFPEIEVFIMVSCELTALM-DGRDYMQPIITPYEATIAF 370 Ostreococcus lucimarinus CCE9901
XP_967520    267 LTLHSKKYYLVSVGKPTVAKLANFPEIDIYVVVTCALNEIY-DSRDYYKPIVTIYDVEMAL 326 red flour beetle
XP_002432293 292 ILKKGKKCYKLAVGKPNVAKLANFPEIDVFVFISCQENLLLkDFKGYYKPIVSMIEIEMAL 352 human body louse
EFN72571     269 LKQKNKKSYILSVGKINPAKLANFPEIDVFVVITCPENEVF-DSRDFLKPMLTPYEVELAF 328 Florida carpenter ant
NP_001040357 289 CKVNGKKSYMVSVGKPNSAKLANFPEMDIYVMMACPENDLY-SSRDFYRPIVFPYELEIAL 348 domestic silkworm
XP_001949323 267 CKKTNKKCYIFSVGRPNVAKLANFPEIEIFVNITCAEGVIE-NQKEYMQPIVNIDDIELAL 326 pea aphid
XP_309119    299 AKARGVRTYLISIGKVNPEKLANFIDIDCFVLVGCPENDVF-TSREFFRPLLSVFEAEMGL 358 Anopheles gambiae str. PEST
XP_001863593 288 AKARGIRSYIISVGKINPAKLANFAEIDCFVLVGCPENDLF-TSRDFFKPLVSVFECELAF 347 southern house mosquito
XP_002097817 294 AKGRGIKTQLISVGRINPAKLANFLEIDCFVLIGCPFNNMY-DSKEYYKPIVSVFEAEMAL 353 Drosophila yakuba
XP_001022303 295 IKYANKKFYTFYMSNLNEAKLNNFPEVEAFVIVSCYRNSLF-DLKKYYKLIVTPFELEMAL 354 Tetrahymena thermophila
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