1KOP,1Y7W


Conserved Protein Domain Family
alpha_CA_prokaryotic_like

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cd03124: alpha_CA_prokaryotic_like 
Click on image for an interactive view with Cn3D
Carbonic anhydrase alpha, prokaryotic-like subfamily. Carbonic anhydrases (CAs) are zinc-containing enzymes that catalyze the reversible hydration of carbon dioxide in a two-step mechanism: a nucleophilic attack of a zinc-bound hydroxide ion on carbon dioxide, followed by the regeneration of the active site by ionization of the zinc-bound water molecule and removal of a proton from the active site. They are ubiquitous enzymes involved in fundamental processes like photosynthesis, respiration, pH homeostasis and ion transport. Most alpha CAs are monomeric enzymes. The zinc ion is complexed by three histidines. This sub-family includes bacterial carbonic anhydrase alpha, as well as plant enzymes such as tobacco nectarin III and yam dioscorin and, carbonic anhydrases from molluscs, such as nacrein, which are part of the organic matrix layer in shells. Other members of this family may be involved in maintaining pH balance, in facilitating transport of carbon dioxide or carbonic acid, or in sensing carbon dioxide levels in the environment. Dioscorin is the major storage protein of yam tubers and may play a role as an antioxidant. Tobacco Nectarin may play a role in the maintenace of pH and oxidative balance in nectar. Mollusc nacrein may participate in calcium carbonate crystal formation of the nacreous layer. This subfamily also includes three alpha carbonic anhydrases from Chlamydomonas reinhardtii (CAH 1-3). CAHs1-2 are localized in the periplasmic space. CAH1 faciliates the movement of carbon dioxide across the plasma membrane when the medium is alkaline. CAH3 is localized to the thylakoid lumen and provides CO2 to Rubisco.
Statistics
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PSSM-Id: 239398
Aligned: 71 rows
Threshold Bit Score: 106.202
Created: 31-Oct-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
active sitezinc binding
Conserved site includes 7 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1Y7W: a halotolerant carbonic anhydrase from Dunaliella salina binds Zinc and acetic acid
  • Comment:zinc binding histidines and other residues found to be important for enzymatic activity

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                               #       
1KOP_A       11 SPESWgnls----eefrLCStGKNQSPVNIT-------ETVSGk----LPAIKVnykp-----smvDVENNgHTIQVNYp 70  Neisseria gonorr...
1Y7W_B       28 HGFDWpglqeggttkypACS-GSNQSPIDINtnqlmepSSRSGtsavsLNGLNVdgaqadgitltnAKVDLeQGMKVTFd 106 Dunaliella salina
BAA11940     61 GPHYWhtis----kcfiACGiGQRQSPINIVs-----yDAKFRq---rLPKLKFkphm----eklkTEVTNhQNRAPEFe 124 Pinctada fucata
ZP_00323232   9 QQDQWe-----------SEA-RETQSPILIEta---klKAKKNq----IVDGLKnyt-------atELHKNyNSLSLTAn 62  Pediococcus pent...
ZP_00319951   2 PKLDYdnq------nnwLFKtGQMQSPVALEsn----kAEKIEkq--aTLKLNYstn-------asYVHDNgQGIEIGLs 62  Oenococcus oeni ...
ZP_00063288   8 EQEDWr----------kADD-TIYQSPIDIPlt---siQQQEN-----SQLIIHfed-------rvQSDDRiIGEQFLVq 61  Leuconostoc mese...
NP_786120     7 HQTDWp-----------MTS-GQHQSPIDIQt-----qHVQSSq----LSAINWrgl-------yqAKTITgEATTLKAs 58  Lactobacillus pl...
ZP_00459026  42 HGFDYdhqr-----ewhLES-GDAQSPVAIDsat-vkrATRYE-----DENVAIdvhia---dtraKAIDNgHTIQIVPa 106 Burkholderia cen...
ZP_00369682  33 DKDKWknld----knwiYCEsGLNQDFININs-----kKATSK-----NHPLEFnysn-----dsfGLINDgYTIKMHFa 93  Campylobacter la...
CAH58714     48 GPGGWgdlk----aewgVCKsGSRQSPIAITa-----lDLVTDr---sLGKLDAkyrk----rvhaTLYNSgHGAEVSMp 111 Physcomitrella p...
Feature 1                              # # #         #              #                           
1KOP_A       71 e---ggNTLTVng----rTYTLKQFHFHV------PSENQIKGRTf--PMEAHFVHLde------------------nKQ 117 Neisseria gonorr...
1Y7W_B      107 qp--aaNLPTIeiggttkSFVPIQFHFHHf-----LSEHTINGIHy--PLELHIVMQeqdpa------------dvatAQ 165 Dunaliella salina
BAA11940    125 pedgenLYVKLnnl-vdgHYKFHNLHVHNgrtrrkGSEHSVNGRFt--PMEAHLVFHhddqthfeptrtklggafpghND 201 Pinctada fucata
ZP_00323232  63 ------GKMEAdq----rSFQLEEVHFHH------PAEHCFKGEAtprVLEAHFVHYgc------------------lRQ 108 Pediococcus pent...
ZP_00319951  63 ------GKAIIdn----rFFSLQQFHIHA------PSEHLLNNYRf--DGEIHFVHQak------------------dGR 106 Oenococcus oeni ...
ZP_00063288  62 ------GNLRVdg----tVWALERFHFHD------GAEHLVDGKRy--DAEIHFVFQr-------------------dNQ 104 Leuconostoc mese...
NP_786120    59 g----iGAAYLnd----rDFAFQQIHFHT------PAEHLIDGQAa--PIEWHLVHQsa------------------tGQ 104 Lactobacillus pl...
ZP_00459026 107 -----gGSATIrg----rHFRLQQIHFHA------PAEHTLDGRSs--PIEGHFVFRsq------------------dGR 151 Burkholderia cen...
ZP_00369682  94 s---ngSHIVLdd----tSYNLSHFHFHS------PSKISIDKKYy--PLEIHFSHVsq------------------nGN 140 Campylobacter la...
CAH58714    112 a---gsGRLRIgg----eTYRPVQFHIHM------PSEHTIMNQSf--PLELHLVHKsd------------------dGK 158 Physcomitrella p...
Feature 1                                                                                       
1KOP_A      118 PLVLAVLYEag-------ktNGRLSSIWNVMpmta--------------------------------------------- 145 Neisseria gonorr...
1Y7W_B      166 LAVIGIMYKyse------ngDAFLNSLQTQIegkigdgt----------------------------------------- 198 Dunaliella salina
BAA11940    202 FVVVGVFLEvgddgfgdepdDEECKHILKGHhpdnnengngdngnngyngdngnngdngnnsyngdngnngvngnngyng 281 Pinctada fucata
ZP_00323232 109 PLVVSVTFKlg-------akNATFEKILTAMd------------------------------------------------ 133 Pediococcus pent...
ZP_00319951 107 TAVIAVFLKag-------ktSKTFSQIFNHLn------------------------------------------------ 131 Oenococcus oeni ...
ZP_00063288 105 TLVLAVFGEt---------aDTDNGIEVRNIy------------------------------------------------ 127 Leuconostoc mese...
NP_786120   105 LAVVAVFGRig-------kpNSSLQGLLDQFtaq---------------------------------------------- 131 Lactobacillus pl...
ZP_00459026 152 LAVVAVFYRtg-------seNAQFAAVMDALrga---------------------------------------------- 178 Burkholderia cen...
ZP_00369682 141 IAVVVLFLQeg-------aeNPFIKKIIRAFpkke--------------------------------------------- 168 Campylobacter la...
CAH58714    159 LAVIGFLFEeg-------geSEFLAQFAHEVpssn--------------------------------------------- 186 Physcomitrella p...
Feature 1                                                                                       
1KOP_A      146 --------------------------------------------------------------------------gkvKLN 151 Neisseria gonorr...
1Y7W_B      199 ----------------------------------------------------------------------asygdtgVSI 208 Dunaliella salina
BAA11940    282 dngnngdngnngyngdngnngdngnngengnngengnngenghkhgcrvkkakhlsrilecayrndkvrefkkvgeeEGL 361 Pinctada fucata
ZP_00323232 134 ----------------------------------------------------------------------------dNKV 137 Pediococcus pent...
ZP_00319951 132 ----------------------------------------------------------------------------qDQN 135 Oenococcus oeni ...
ZP_00063288 128 ----------------------------------------------------------------------------dEHV 131 Leuconostoc mese...
NP_786120   132 --------------------------------------------------------------------------tshELT 137 Lactobacillus pl...
ZP_00459026 179 ---------------------------------------------------------------------------dtASV 183 Burkholderia cen...
ZP_00369682 169 --------------------------------------------------------------------------gdkLYV 174 Campylobacter la...
CAH58714    187 --------------------------------------------------------------------------spgVKV 192 Physcomitrella p...
Feature 1                                #                                                      
1KOP_A      152 QPF-DASTLLPkr--lKYYRFAGSLTTP-PCTEGVSWLVLK-TYDHIDQAQAEKFTRav---------gSENNRPVQPLN 217 Neisseria gonorr...
1Y7W_B      209 DNInVKTQLLPss--lKYAGYDGSLTTP-GCDERVKWHVFT-TPREVTREQMKLFVDvtmga--hagadVVNNRMIQDLG 282 Dunaliella salina
BAA11940    362 DVHlTPEMALPplkyrHYYTYEGSLTTP-PCTESVLWVVQK-CHVQVSRRVLHALRNvegykdgttlrkYGTRRPTQKNK 439 Pinctada fucata
ZP_00323232 138 EQFiLLDDLIPkr--gYFYRYIGSLTTP-PLTEGVEWVVFE-QAQMIDIDQLNLYMAtf---------kEQNNRELQKLN 204 Pediococcus pent...
ZP_00319951 136 FVC-DLTDLIPen--kSYYHYLGSLTTP-PLTENVEWYILA-NPVEISRQQLAEFHKl----------yPYNNRQLQSLN 200 Oenococcus oeni ...
ZP_00063288 132 NAD-GLMKLIPdn--rSYYRYTGSLTTP-PLGQNINWLILT-SAIQISKQDKAFLHAd----------yPNNYREVQDLN 196 Leuconostoc mese...
NP_786120   138 TPV-NLTELLPdt--gTVYHYLGSLTTP-PLSETVEWYVCA-DTVMIGEQQLAAYQQl----------fAANNRKIQPLN 202 Lactobacillus pl...
ZP_00459026 184 PSF-HAAALMPrs-vkTYYHYLGSLTTP-PLTENVEWYVLD-EPVELSADDITAFRMr----------ySHNARVVQPLN 249 Burkholderia cen...
ZP_00369682 175 QGL-SANELLPn---nLQSFYIFKNKLNkPCNQKITWIVLK-DISYASKEQIQIIQNlm---------gKNENLKIQEIN 240 Campylobacter la...
CAH58714    193 DLG-HIKMMKPe---rNYGTYMGSLTTP-PCAEGVTWILSLfNFQTASAEQLAKLRAsv--------pkGHNNRPTFGSA 259 Physcomitrella p...
Feature 1             
1KOP_A      218 ARVVIE 223 Neisseria gonorrhoeae
1Y7W_B      283 DREVYK 288 Dunaliella salina
BAA11940    440 VTVYKS 445 Pinctada fucata
ZP_00323232 205 HRKIEK 210 Pediococcus pentosaceus ATCC 25745
ZP_00319951 201 GRPVLY 206 Oenococcus oeni PSU-1
ZP_00063288 197 GREIDF 202 Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293
NP_786120   203 DRPIIA 208 Lactobacillus plantarum WCFS1
ZP_00459026 250 GRPLLR 255 Burkholderia cenocepacia HI2424
ZP_00369682 241 NLEQND 246 Campylobacter lari RM2100
CAH58714    260 GRGFRM 265 Physcomitrella patens

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