4HER,1RXU,4HER,3QPB,1NW4,3C74,1LX7


Conserved Protein Domain Family
UP_EcUdp-like
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cd17767: UP_EcUdp-like 
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uridine phosphorylases similar to Escherichia coli Udp and related phosphorylases
Uridine phosphorylase (UP) is specific for pyrimidines, and is involved in pyrimidine salvage and in the maintenance of uridine homeostasis. In addition to E. coli Udp, this subfamily includes Shewanella oneidensis MR-1 UP and Plasmodium falciparum purine nucleoside phosphorylase (PfPNP). PfPNP is an outlier in terms of genetic distance from the other families of PNPs. PfPNP is catalytically active for inosine and guanosine, and in addition, has a weak UP activity. This subfamily belongs to the nucleoside phosphorylase-I (NP-I) family, whose members accept a range of purine nucleosides as well as the pyrimidine nucleoside uridine. The NP-1 family includes phosphorolytic nucleosidases, such as purine nucleoside phosphorylase (PNPs, EC. 2.4.2.1), uridine phosphorylase (UP, EC 2.4.2.3), and 5'-deoxy-5'-methylthioadenosine phosphorylase (MTAP, EC 2.4.2.28), and hydrolytic nucleosidases, such as AMP nucleosidase (AMN, EC 3.2.2.4), and 5'-methylthioadenosine/S-adenosylhomocysteine (MTA/SAH) nucleosidase (MTAN, EC 3.2.2.16). The NP-I family is distinct from nucleoside phosphorylase-II, which belongs to a different structural family.
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Statistics
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PSSM-Id: 350167
Aligned: 273 rows
Threshold Bit Score: 221.934
Created: 30-Jun-2017
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 17 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:1RXU_A; Escherichia coli UP homodimer bound with thymidine and phosphate in two active sites; contacts determined at 4A.
  • Comment:the neighboring subunit contributes residue(s) to the protein-ligand interactions
  • Structure:1NW4: Plasmodium falciparum PNP homodimer bound with immucillin-H and sulfate in two active sites; contacts at 4A
  • Comment:Immucillin-H serves as a ligand to stabilize the catalytic site
  • Structure:3QPB: Streptococcus pyogenes UP homodimer bound with ribose-1-phosphate and uracil in two active sites; contacts at 4A
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                        ##  #                                       #                
4HER_A      5 FHLGLTKAMldgaTLAIVPGDPERVKRIAELM-DNATFLASHREYTSYLAYADGKPVVICSTGIGGPSTSIAVEELAQLG 83  Shewanella oneiden...
1RXU_A      7 FHLGLTKNDlqgaTLAIVPGDPDRVEKIAALM-DKPVKLASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLG 85  Escherichia coli
AEB07901   13 YHLHTEPGDi--aPFILTSGSRQRIRRLSALF-DTISFERENREFLTVTGTYRNIPITGMSTGIGASSTVIAIVEALQCQ 89  Desulfobacca aceto...
OEU74588   18 YHIGLRPDDi--sDKIILVGDPDRARRVADLF-ESVRVSRQNREYVTFTGTYKGLELTVMATGIGCDNTEIAVVELCQLK 94  Desulfuromonadales...
OGN83966   17 YHIALGPGEl--aEYILLPGDPDRTATIAARL-DSIELERRHREFASVTGFYRGERVSIVSTGIGTDNVEIVVAEILAIT 93  Chloroflexi bacter...
OGQ23425   15 YHIGLAPGEv--aPFILLCGDPKRARRIATYF-KKARPEITNREYVTITGEYQGIPLTVMATGMGPDNTEMAFVELTQIV 91  Deltaproteobacteri...
EDM77594   17 YHIGLRPGDv--aPAILLVGDPKRAKRVAEGFfDDYGDPITEREFVTYTGTYRGMPMSVMGTGMSAANTEIAVIELCQLF 94  Plesiocystis pacif...
OGT22484   21 YHIDLAPGEl--aEYIVLVGEAKRAERAAKLL-TDIRIERYNREYHTFTGRYKGLEISIMSTGMGPGCTEIGVIEIFQIT 97  Gammaproteobacteri...
SFD80015   19 YHIALKPGEv--aRHVILVGDPERARRVAGNF-EAVELERSNREYVTITGRHDGLRVSVMGTGMGAASTEIAIVELCQCV 95  Nannocystis exedens
OPX20023   14 YHLQVGAGEv--aPLVLTSGSAERIRRLAESF-DRVELVRQQREFLTITGSYQGIRITGLATGIGPDNTAIAVIEAVQYQ 90  Desulfobacca sp. 4...

Feature 1              ######                                                                 
4HER_A     84 v----NTFLRVGTTGAIQPhvNVGDVIVTQASVRLDGASLHFapmeFPAVANFECTTAMVAACrdagvePHIGVTASSDT 159 Shewanella oneiden...
1RXU_A     86 i----RTFLRIGTTGAIQPhiNVGDVLVTTASVRLDGASLHFapleFPAVADFECTTALVEAAksigatTHVGVTASSDT 161 Escherichia coli
AEB07901   90 p---qATFIRLGSCGSLQShiQVGEFVISHRALRQEFVTHLYappeIEAVADPDITQALIQAArqmgvsSHCGLTCTTAD 166 Desulfobacca aceto...
OEU74588   95 f---pVTIIRCGSCGALQSdiEIEDLVISSGAVRLENTSTFFvepgYPAVASHEVILALLKSAaneklpHHFGMTATAPG 171 Desulfuromonadales...
OGN83966   94 q---rPVFIRVGSCGALQPdiALGDLVISSGAVRLETTTSWFvhegYPAIAHHEALVALIETAerlghrYHVGVTATAPG 170 Chloroflexi bacter...
OGQ23425   92 k---sPTMIRVGTSGGLSRklQLGDQVISTGAVRLENTSTFFvsegYPALAHHECILALIEAAerkkvsYHVGVTATASG 168 Deltaproteobacteri...
EDM77594   95 edassVAIIRAGSCGALQAniGLGDLVITQAAHRLEETSLAFvtpgYPAVAHPEVVLALAWACedtgaaHHLGITATAAG 174 Plesiocystis pacif...
OGT22484   98 k---nPTLLRVGTCGSLQPkmEVGDLIISTGAVRLEDTSTYFvhegYPAIANHEAVLALTMAAdkldikYHLGITATGSG 174 Gammaproteobacteri...
SFD80015   96 e---rPVMIRCGSTGALQPgmALGDLVISQAALRLENTTRFYvedsYPAVADPQAVMALAAAAdaigqrYHVGVTATSPS 172 Nannocystis exedens
OPX20023   91 p---qATFIRIGSCGSLQPeiQLGDLIISTEALRHENTTQFYapaeLKAVADPLVTQALIAAAqeqgvvYHCGLTCTTSD 167 Desulfobacca sp. 4...

Feature 1     #                                ####                       ##                  
4HER_A    160 FYpgqerydtvtgrvtrrfagsMKEWQDMGVLNYEMESATLFTMCATQG--WRAACVAGVIVNrtqqeipdeatMKKTEV 237 Shewanella oneiden...
1RXU_A    162 FYpgqerydtysgrvvrhfkgsMEEWQAMGVMNYEMESATLLTMCASQG--LRAGMVAGVIVNrtqqeipnaetMKQTES 239 Escherichia coli
AEB07901  167 FYhgqgraapg---ftgfdsslLANLQVQGVLNLEMEMAVYLTLAQVCQypMRAGGVTAVFADrcrnviidpqqATEAEE 243 Desulfobacca aceto...
OEU74588  172 FYgaqgrhipg---fpakdealMDRLTKQGIKNFEMESSTLFTLASLRG--FRAGTICAVFASrprdrfidpvkKGKAEL 246 Desulfuromonadales...
OGN83966  171 FYgaqgrpipq---lpirfpdlAEEMTRQRVLNFEMEAAAVMVLAGLAG--CRAGVVCAVYAQrtsgefldeaaRKRADG 245 Chloroflexi bacter...
OGQ23425  169 FYgaqgrkipn---lpsrvpnlPATLDAMNVLNFEMEASCLFSLGTLAG--IRTGAICTTVANrhfnifmqkedILRAEK 243 Deltaproteobacteri...
EDM77594  175 FYgaqgrnipg---fpsrvpaiEAELQRMGISNLEMEASTLLTLSTLRG--FRAGAVCAVYANrvdntfiadadKSAAEK 249 Plesiocystis pacif...
OGT22484  175 FYgaqgrdipg---ihirypnlPDELAKMNVANFEMEASTLFTLCAMKG--CRASAICTAVANrykktfisneqKSAKEK 249 Gammaproteobacteri...
SFD80015  173 FYagqgraiag---fpprepdiVERLARQGVKNLEMETACLLTLATLRG--FVAGAVCAVYATraddvfispaqKDMAEK 247 Nannocystis exedens
OPX20023  168 FYhgqgrrvpg---fsgcdptlLPRLQAKGVLNLEMEMAAYFILAQVSDfpLRAGGLTAVFADrwrgtfiapeeMSAAED 244 Desulfobacca sp. 4...

Feature 1                
4HER_A    238 SAVSIVVAAAK 248 Shewanella oneidensis MR-1
1RXU_A    240 HAVKIVVEAAR 250 Escherichia coli
AEB07901  244 LLCRIGLLAAE 254 Desulfobacca acetoxidans DSM 11109
OEU74588  247 RAIKTALGAFE 257 Desulfuromonadales bacterium C00003093
OGN83966  246 TVVETGLEALV 256 Chloroflexi bacterium GWC2_73_18
OGQ23425  244 ECLEAGLEAFV 254 Deltaproteobacteria bacterium RIFCSPHIGHO2_02_FULL_44_16
EDM77594  250 RCIQAGLEGLL 260 Plesiocystis pacifica SIR-1
OGT22484  250 IALTAGLDALL 260 Gammaproteobacteria bacterium RIFCSPHIGHO2_02_FULL_42_13
SFD80015  248 ACIATGLGALH 258 Nannocystis exedens
OPX20023  245 RAIQVALGAVL 255 Desulfobacca sp. 4484_104

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