3KXW,3LNV,3PBK,5EY8,5EY9,5HM3,5ICR


Conserved Protein Domain Family
FAAL

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cd05931: FAAL 
Click on image for an interactive view with Cn3D
Fatty acyl-AMP ligase (FAAL)
FAAL belongs to the class I adenylate forming enzyme family and is homologous to fatty acyl-coenzyme A (CoA) ligases (FACLs). However, FAALs produce only the acyl adenylate and are unable to perform the thioester-forming reaction, while FACLs perform a two-step catalytic reaction; AMP ligation followed by CoA ligation using ATP and CoA as cofactors. FAALs have insertion motifs between the N-terminal and C-terminal subdomains that distinguish them from the FACLs. This insertion motif precludes the binding of CoA, thus preventing CoA ligation. It has been suggested that the acyl adenylates serve as substrates for multifunctional polyketide synthases to permit synthesis of complex lipids such as phthiocerol dimycocerosate, sulfolipids, mycolic acids, and mycobactin.
Statistics
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PSSM-Id: 341254
Aligned: 56 rows
Threshold Bit Score: 476.731
Created: 25-Jan-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
active siteacyl-activating
Conserved site includes 19 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:3KXW; Legionella pneumophila Fatty acid AMP ligase binds acyl adenylate, contacts at 4A
  • Structure:3LNV; Legionella pneumophila Fatty acyl-adenylate ligase binds acyl adenylate and pyrophosphate, contacts at 4A

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                         
3KXW_A         19 LRALHSPNkk----scTFLNKE--------LEETXT--YEQLDQHAKAIAATLQAEga---kPGDRVLLLfAPGLPLIQA 81   Legionella pn...
AAC82779       10 RLIESLEDvagamhrmTFVDDAg-------HEAGIT--YRHFAEEVFRQAGALRELgv---rENDLVMLAlPASVEHAAA 77   Burkholderia ...
BAD68929       21 PVCAKHPGlgsk-pafIWADDQagggsgvsPRETLT--YSELDSAVQRMAAGLLEVl----rRGDTVLVLaSPGIRLVKL 93   Japanese rice
CAN60763       25 NLPAFRSKa-------AFIWAE--------DGATLT--YAQLNSSAQSISAKILHRl----kRGDTVVILcSPGLELVEI 83   wine grape
A0R3D6         10 RNARTVTT--------GMVTGEp------hEPVRHT--WGEVHERARRIAGGLAAAgi---gHGDAVGVLaGFPVEIAPT 70   Mycobacterium...
P9WQ40         11 SMQASAGDl-------MVLDRE--------TSLWCRhpWPEVHGLAESVCGWLLDHd-----RPAAVGLVgEPTVELVAA 70   Mycobacterium...
Q1BBA2          4 ALADAMTSsgr---dlVVLDHG--------GWRRHP--WAEVHQRAENIAARITED------AATVVVLVgEPTVEFIAA 64   Mycobacterium...
XP_014621790  499 KHWGTHPItqnr-tlyTWLNEGg------tVVGKRT--YGEQHLNASCIAHKLLTSqkpvikPGDRVLLVyVPGLDFVDA 569  soybean
YP_177686      13 QQISACTD--------RTLTITc------eDSEVIS--YQDLIARAAACIPPLRRLdl---kRGEPVLITaHTNLEFLSC 73   Mycobacterium...
YP_003770343   10 ATATKRGQq------rGMVTGEp------kEPVRRT--WAEVHEEARRIAGGLVAGgf---eRGTAVGVL-AAAPVLIAP 71   Amycolatopsis...
Feature 1                                                                                         
3KXW_A         82 FLGC-LYAGCIAVPIYPPaqe--------kllDKAQRIVTNS-----KPVIVLXIADhikkftadelnt----------- 136  Legionella pn...
AAC82779       78 MMAC-VMTGALPCTVPVPvrra-------aagRQVADVACELy----RPRLVVAADAqaaawrdda-------------- 131  Burkholderia ...
BAD68929       94 MFAC-QRAGLVAVPVIPPdpsvigtpaegpahRHLLRAVSQA-----RPRAAVADAGyvgavtrssisalm--------s 159  Japanese rice
CAN60763       84 IFGC-QRAGLLSVPIFPPhpsft-----kedhYHLVRVLSQT-----KPKAAIAQHGfiaavkryvsspvsdk----rlc 148  wine grape
A0R3D6         71 AQGV-WMRGASLTMLHQPtprtd----lavwaEDTMNVIGMI-----ELKAVIISEPflvatpvlee------------- 127  Mycobacterium...
P9WQ40         71 IQGA-WLAGAAVSILPGPvrg--------andQRWADATLTRfl-giGVRTVLSQGSylar------------------- 121  Mycobacterium...
Q1BBA2         65 IPGT-LYAGAALSILPGPvrga-------dlgQWARNTMQRCas--iGVRLVFSHGEhldalrat--------------- 119  Mycobacterium...
XP_014621790  570 FFGC-LRAKVLPVPVLPPdpmqr----ggqalLKIENIAKSC-----GIVAILSTASyhtavraglvkslisltgkngky 639  soybean
YP_177686      74 FLGL-MLHGAVPVPIPPReal--------kttERFMTRLGPLl---rHHRVLICTPAehdeiraaastdcqisr-ftala 140  Mycobacterium...
YP_003770343   72 TVQAvWLAGGSVTMLHQPtprtdla-ewaedtVRVLGMIGSDqdalgGPGGLAPRPGsgagprnvtvllgepfd-qlapv 149  Amycolatopsis...
Feature 1                                                                              #          
3KXW_A        137 npkflkipaialESIELNRSSSWQpts-------ikSNDIAFLQYTSGSTXHPKGVXVSHHNLLDNLNKIFTsfh--xnD 207  Legionella pn...
AAC82779      132 ---fpaaatrvvDLAMLSSAADAGarali---ssksGRDPHHVQLTSGSTSHPKAAVLSHENVIANVLGIGGsvrfdiaA 205  Burkholderia ...
BAD68929      160 lnwvsvdrlerwPAVASTVAATGDgvye-----gcgPRETYLVQYTSGATGDPKPVVVTAGAAAHNARAARKayd--lrP 232  Japanese rice
CAN60763      149 ellqgvrwistdDVKDGKEGNSSSgslpy---hgckADEMYLVQFTSGATGIPKPVLVTAGAAAHNVRTARKayh--lhP 223  wine grape
A0R3D6        128 ---kgilvlkvaDLLAADPIDPVEt----------gEDDLALMQLTSGSTGSPKAVQITHRNIHSNAEAMFVgak-ydvE 193  Mycobacterium...
P9WQ40        122 --------lrsvDTAGVTIGDLSTaahtnrsatpvaSEGPAVLQGTAGSTGAPRTAILSPGAVLSNLRGLNQrvg-tdaA 192  Mycobacterium...
Q1BBA2        120 -----pasiptcDVTETGHARRDSsft------ppdHGEFAVLQGTAGSTGIPRTAQLSPEAVLANLRGLVAcid--itP 186  Mycobacterium...
XP_014621790  640 taqwpklpwlhtDTWVNNSRNSAVedvdd--qcepqPGDVCFLQFTSGSTGDAKGVMITHGGLVHNVKLMRSryk--stS 715  soybean
YP_177686     141 eagdeqfgrataQQLADTATADWPlc---------tLDDDAYVQYTSGSTAAPRGVVITYRNLLSNMRAMAVgsq--fqH 209  Mycobacterium...
YP_003770343  150 leqkgigfqlitELAAAEPLPDVVvt---------dEGDTALLQLTSGSTADPKAVRITYGNLYSNVKAMVErae-fdfD 219  Amycolatopsis...
Feature 1                    ###                                                                  
3KXW_A        208 ETIIFSWLPpHHDXGLiGCILTPIYgGIQAIXXSPf-SFLQNPLSWLKHITKYKATISGSPNFAYDYCVKRiree--kke 284  Legionella pn...
AAC82779      206 GDGTASWLPlYHDMGL-LTLLSNLHyRAPLLMMQPn-SFIRNPLGWLKRIASARATTTSVPTFALRYCVRRfnaa--amd 281  Burkholderia ...
BAD68929      233 GSVVVSWLPqYHDCGL-MFLLLTVVsGATCVLTSAs-AFVRRPRLWLELISEFKATCTPVPSFALPLVLRRgrseh-gtr 309  Japanese rice
CAN60763      224 NSVIVSWLPqYHDCGL-MFLLLTIVsGATCVLTSPi-AFVNRPRLWLELISEYKATCTPVPSFALPLVVKRggidk-gtl 300  wine grape
A0R3D6        194 KDVMVSWLPcFHDMGMvGFLTIPMYfGAELVKVTPm-DFLRDTLLWAKLIDKYKGTMTAAPNFAYALLAKRlrrqa-kpg 271  Mycobacterium...
P9WQ40        193 TDVGCSWLPlYHDMGL-AFVLSAALaGAPLWLAPTt-AFTASPFRWLSWLSDSGATMTAAPNFAYNLIGKYarr----vs 266  Mycobacterium...
Q1BBA2        187 ADTGCSWLPlYHDMGL-AFLLTNAL-GGAELWQAPttAFSASPFSWLQWMTESTATLTAAPNMAYGLIGKYgsr----lt 260  Mycobacterium...
XP_014621790  716 RTVLVSWLPqYHDMGLiGGLFTALVsGGSAVLFSPl-TFIKKPLLWLETISKYQATHSAGPNFAFELVVRRlesekdklq 794  soybean
YP_177686     210 GDVMGSWLPlHHDMGLvGSLFAALFnSVSAVFTTPh-RFLYDPLGFLRLLTSSGATHTFMPNFALEWLINAyhrrgadie 288  Mycobacterium...
YP_003770343  220 VDVMVSWLPtFHDMGMvGFLTVPMTfGVELVKITPv-EFLSGPLIWPELITKYHGTTTAAPNFAYAIVGRRmarvd-edd 297  Amycolatopsis...
Feature 1                   #  ####                        #####    #                             
3KXW_A        285 gLDLSSWVTAFNGAEPVREETXEHFYQAFKEFGFRKEAFYPCYGLAEATLLVTGGTPgssyktltlakeqfqd------- 357  Legionella pn...
AAC82779      282 gVDLSACRNIFIGGERVDDTTLRDFAATFAPYGLAASALQPCYGMAESTLAVSMHRAwhegavdgapyviadtldrrali 361  Burkholderia ...
BAD68929      310 pLQLQTLRNLILVNEPIYKSPVDEFLEEFGPAGLRASSISPSYGLAENCTFVSTAWRgtevtl----------------- 372  Japanese rice
CAN60763      301 pINLWSLKNLIIINEPIYRDSVEEFVNAFLPFGLNPACISPSYGLAENCTFVSTAWRnnggsssf--------------- 365  wine grape
A0R3D6        272 dFDLSTLRFALSGAEPVEPADVEDFLDAGKPFGLRPEAILPAYGMAETTLAVSFSPCgeglvvdevdadlla-------- 343  Mycobacterium...
P9WQ40        267 eVDLGALRVTLNGGEPVDCDGLTRFAEAMAPFGFDAGAVLPSYGLAESTCAVTVPVPgiglladrv-------------- 332  Mycobacterium...
Q1BBA2        261 dFDLGALRFALNGGEPVDCQGYRRFATEMARFGLDPSALVPAYGLAESSCAVTVPILgsglqvdevqv------------ 328  Mycobacterium...
XP_014621790  795 sLDLSSMIFLMVAAEPVRMKTLKRFLDLTTPFGLSQKVMAPGYGLAENCVFVSCAFGegcpil----------------- 857  soybean
YP_177686     289 gIDLHKMRRLIIASEPVHAEGMRRFAATFAGVGLAPTALGSGYGLAEATVAVSMSAPntgfrte---------------- 352  Mycobacterium...
YP_003770343  298 aYDLSKLRIALNGAEPIDETAVQTFVDAGARFKMPAECVFPAYGMAEATLAVSFAPLftgltldvveadaleaenr---- 373  Amycolatopsis...
Feature 1                                #                                                        
3KXW_A        358 -----hrvhfaddnspgsykLVSSGNPI---------QEVKIIDPdtlip-cdfdQVGEIWVQSNSVAKGYWNQPeetrh 422  Legionella pn...
AAC82779      362 errdaqpaaandgqdtesetVLAMGTPId-------gMAFRILDDggha--lasrAVGEVAIRGTSVMLGYLNPDdgtia 432  Burkholderia ...
BAD68929      373 --------------psykklLPSARLPQpsllteapdVEIVVVDAetgkl-vedgVEGEIWLSSPSNGSGYLGDSpasre 437  Japanese rice
CAN60763      366 ------------pnipsynkLLPSARLAseci-deeeMDILVVNEetqep-vedgIEGEIWVSSSSNASGYLGHPsitry 431  wine grape
A0R3D6        344 -----alrravpatkgntkrLASLGPLLn-------dLEARVVDEngev--mparGVGVIELRGEPVTPGYITMGgfvp- 408  Mycobacterium...
P9WQ40        333 ------------idgsgahkHAVLGNPIp-------gMEVRISCGdqaag-nasrEIGEIEIRGASMMAGYLGQQpi--- 389  Mycobacterium...
Q1BBA2        329 ----------ssddgrfirtHAVVGEPIp-------gMQVRIAPTdgsgeqvdgrDFGEIEVRGTSLMSGYLGEPpl--- 388  Mycobacterium...
XP_014621790  858 ---------------vdwqgRVCCGYIHpgd----sdVDIIIVDPesgeeleedgREGEIWISSPSAGIGYWGKEelsqk 918  soybean
YP_177686     353 --------------thaaaeVVTGGRVLp-------gYEVRIDAApg-------aRAGTIKLRGDSVAAKAYVGGkkld- 403  Mycobacterium...
YP_003770343  374 --avpvpegdprrgtdgvrsFALLGRPLd-------gLEAEIVDDagkr--vderEVGEIRLRGEAVTPGYLTMDgpla- 441  Amycolatopsis...
Feature 1                            #            #  #                                            
3KXW_A        423 afagkikddersAIYLRTGDLGFLh--ENELYVTGRIKDLII--IYGKNHYPQDIEFSLXHSPlh-hvLGKCAAFVIQEE 497  Legionella pn...
AAC82779      433 -------apltaDGWFRTGDIGYVa--DGQLHILGRKKEVII--IRGSNYFPHEIEEALASHSal--rKSTCIAFGLPDP 499  Burkholderia ...
BAD68929      438 vfg-arlqgragPCFVRTSDLGVVhgtERYLYVLGRTTDAIVvaDSQRRVPAHYIETAAFESSpgrlrGGCIASFTTLPV 516  Japanese rice
CAN60763      432 vfl-grlsnkvsSCFVRTGDRGIVkgeERYLFITGRSSDIIEl-QSGEQIHPHYIETAAYNSCqkflrGGCLAAFKISRT 509  wine grape
A0R3D6        409 --------aqdeYGWYDTGDLGYIt-eEGNIVVCGRVKDVII--MAGRNIYPTDIERAAGRVEgv--rPGCAVAVRLDAG 475  Mycobacterium...
P9WQ40        390 ----------dpDDWFATGDLGYLg--AGGLVVCGRAKEVIS--IAGRNIFPTEVELVAAQVRgv--rEGAVVALGTGDR 453  Mycobacterium...
Q1BBA2        389 ----------eaGSWFPTGDLGYFv--DSGLVVCGRAKELIT--VAGRNIFPAEVERVAAQVQgv--rEGAVVAVGTDES 452  Mycobacterium...
XP_014621790  919 tfr-nklqnhpgRNYTRTGDLGRIi--DGKLFITGRIKDLII--VAGRNIYSADVEKTVESSSef-lrPGCCAVIGVPEE 992  soybean
YP_177686     404 --------aldeEGFCDTHDLGFLv--DDEIVILGRQDEVFI--VHGENRFPYDIEFIIRGESeq--hRTKVACFGVNER 469  Mycobacterium...
YP_003770343  442 --------tqdeDGWLNTGDLGYLv--DGQIVICGRRKDVII--MGGRNLYPTDIERAATSVEgv--rAGNAVAVRLDAG 507  Amycolatopsis...
Feature 1                                                                                         
3KXW_A        498 HEYKLTVXCEvknrfxd----------dvaqdnLFNEIFELVYEnHQLEVHTIVLipLKAXPHTTSGKIRRNFCRKHLLD 567  Legionella pn...
AAC82779      500 ETGTERLVVAiearpv------------datpqTRTECQQLLASrIGFAAQDLCFvePGSLPRTTSGKLQRLKCRDLYAD 567  Burkholderia ...
BAD68929      517 SPSPVVVVAElqnggg-----------ggdmagICHGIRQAVWReVGVTVARVVLaeSGGVPKTTSGKLQRGAARAKLLA 585  Japanese rice
CAN60763      510 VVLIAEMQRReeetr--------------ilkiICEGIRDSVLReEKIGIGLVVLvkAGCVPKTTSGKIQRWATKDKLIG 575  wine grape
A0R3D6        476 HSRETFAVAVesnawqd----------paevrrIEHQVAHEVVSeVDVRPRNVVVlgPGSIPKTPSGKLRRANSVSLVTQ 545  Mycobacterium...
P9WQ40        454 STRPGLVVAAefrgp--------------deanARAELIQRVASeCGIVPSDVVFvsPGSLPRTSSGKLRRLAVRRSLEM 519  Mycobacterium...
Q1BBA2        453 AVRPGLVIAAefrgt--------------depaARTELVQRVASeCGVVPSNVVFlePGALPRTSSGKLRRLEVKRSMED 518  Mycobacterium...
XP_014621790  993 ILSAKGISLPdgsdqvglvvvaevrdgktvskdVIEHIQTRVVEeHGVSVASVKLikPRTISKTTSGKIKRFECLKQFSD 1072 soybean
YP_177686     470 VVVVLESPLDsii-----------------dkaEADRLRCQVVAaTGLQLDELITvrRGAIPTTTSGKLKRRAVAQAYRD 532  Mycobacterium...
YP_003770343  508 SRRERFAVVLesklagd----------aeaeknLMKQVSARVRDaVDMRPYAVVVlpAGSLPKTPSGKVKRAATAQQFAD 577  Amycolatopsis...

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