1NVS,3JVR,2BR1


Conserved Protein Domain Family
STKc_Chk1

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cd14069: STKc_Chk1 
Click on image for an interactive view with Cn3D
Catalytic domain of the Serine/Threonine kinase, Checkpoint kinase 1
STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. Chk1 is implicated in many major checkpoints of the cell cycle, providing a link between upstream sensors and the cell cycle engine. It plays an important role in DNA damage response and maintaining genomic stability. Chk1 acts as an effector of the sensor kinase, ATR (ATM and Rad3-related), a member of the PI3K family, which is activated upon DNA replication stress. Chk1 delays mitotic entry in response to replication blocks by inhibiting cyclin dependent kinase (Cdk) activity. In addition, Chk1 contributes to the function of centrosome and spindle-based checkpoints, inhibits firing of origins of DNA replication (Ori), and represses transcription of cell cycle proteins including cyclin B and Cdk1. The Chk1 subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase.
Statistics
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PSSM-Id: 270971
Aligned: 12 rows
Threshold Bit Score: 412.88
Created: 17-Jul-2006
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 18 residues -Click on image for an interactive view with Cn3D
Feature 1:ATP binding site [chemical binding site]
Evidence:
  • Structure:1NVS: Human Chk1 binds inhibitor SB218078; contacts at 4A
  • Structure:2BR1: Human Chk1 binds furanopyrimidine inhibitor; contacts at 4A
  • Comment:also based on structures of other members of the CAMK family bound with ATP or ATP analogs or inhibitors
  • Citation:PMID 9362479

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1             ####    #              # #                                      #       
1NVS_A      7 EDWDLVQTLGEGAYGEVQLAVNRv--tEEAVAVKIVDMkrav------dcpENIKKEICINKMLn---HENVVKFYGHRR 75  human
3JVR_A      6 EDWDLVQTLGEGAYGEVQLAVNRv--tEEAVAVKIVDMkrav------dcpENIKKEICINKMLn---HENVVKFYGHRR 74  human
2BR1_A      7 EDWDLVQTLGEGAYGEVQLAVNRv--tEEAVAVKIVDMkrav------dcpENIKKEICINKMLn---HENVVKFYGHRR 75  human
AAA93318   22 SLYRVVQTLGEGAFGEVLLIVNTkn-pEVAAAMKKINIanks-----kdfiDNIRKEYLLQKRVsavgHDNVIRMIGMRN 95  Caenorhabditis ele...
Q6DE87      7 EDWDLVQTLGEGAYGEVQLAVNRk--tEEAVAVKIVDMtraa------dcpENIKKEICINRMLs---HTNIVRFYGHRR 75  African clawed frog
O61661     20 EGWTLAQTLGEGAYGEVKLLINRq--tGEAVAMKMVDLkkhp------daaNSVRKEVCIQKMLq---DKHILRFFGKRS 88  fruit fly
Q8AYC9      7 EDWDLVQTLGEGAYGEVQLAVNRr--tEEAVAVKIVDMkraa------dcpENIKKEICINKMLn---HENVVKFYGHRR 75  chicken
CAK04818    7 KDWDVVQTLGEGAYGEVRLLVNKk--tEEAVAVKVVDMakak------dciENVKKEVCICKMLs---HPNIVRFFGHRS 75  zebrafish
NP_502989   4 NQFKPIKSIGKGAFGQVFLAEKLnn-pRIKVAMKKINIfgap-----keflEQIQQEYYIQKNMk---HKNIVKMYGMSM 74  nematode
544017      8 FPYHIGREIGTGAFASVRLCYDDn---AKIYAVKFVNKkhatscmnagvwaRRMASEIQLHKLCn--gHKNIIHFYNTAE 82  fission yeast
585344     13 KDVVLGDTVGQGAFACVKNAHLQmd-pSIILAVKFIHVptckk---mglsdKDITKEVVLQSKCs--kHPNVLRLIDCNV 86  baker's yeast
P0C198     16 LDYRIVQLIGGGGFSKVFRAVNPssesHSVAAIKVISYapnrsn-kypidrRALQKEVQVHSILk---HPNVLEFLGAVE 91  Ustilago maydis
Feature 1                                                            ####   #                 
1NVS_A     76 Eg-----------------------------------------------nIQYLFLEYCSgGELFDRIEPdiGMPEPDAQ 108 human
3JVR_A     75 Eg-----------------------------------------------nIQYLFLEYCSgGELFDRIEPdiGMPEPDAQ 107 human
2BR1_A     76 Eg-----------------------------------------------nIQYLFLEYCSgGELFDRIEPdiGMPEPDAQ 108 human
AAA93318   96 Dp-----------------------------------------------qFYYLFLEYADgGELFDKIEPdcGMSPVFAQ 128 Caenorhabditis ele...
Q6DE87     76 Eg-----------------------------------------------nIQYLFLEYCRgGELFDRIEPdvGMPEQDAQ 108 African clawed frog
O61661     89 Qg-----------------------------------------------sVEYIFLEYAAgGELFDRIEPdvGMPQHEAQ 121 fruit fly
Q8AYC9     76 Eg-----------------------------------------------aTQYLFLEYCSgGELFDRIEPdiGMPEPEAQ 108 chicken
CAK04818   76 Eg-----------------------------------------------tTQYIFLEYCSgGELFDRIEPdvGMPEKEAH 108 zebrafish
NP_502989  75 Dt-----------------------------------------------eFCYLFQEFVTgGDLFEKIDFenGMPVNQVK 107 nematode
544017     83 Np-----------------------------------------------qWRWVVLEFAQgGDLFDKIEPdvGIDEDVAQ 115 fission yeast
585344     87 Sk-----------------------------------------------eYMWIILEMADgGDLFDKIEPdvGVDSDVAQ 119 baker's yeast
P0C198     92 RgvdkngnagkekgilmgeavattkesirardkerqqmlsspshdqnyvpGLYMVLELGAgGDLFDKIAPdyGVEEDLAH 171 Ustilago maydis
Feature 1                               ## #         ##                                       
1NVS_A    109 RFFHQLMAGVVYLHG-IGITHRDIKPENLLLDerDNLKISDFGLATVFRYNN-RERLLNKMCGTLPYVAPELLKrr-eFH 185 human
3JVR_A    108 RFFHQLMAGVVYLHG-IGITHRDIKPENLLLDerDNLKISDFGLATVFRYNN-RERLLNKMCGTLPYVAPELLKrr-eFH 184 human
2BR1_A    109 RFFHQLMAGVVYLHG-IGITHRDIKPENLLLDerDNLKISDFGLATVFRYNN-RERLLNKMCGTLPYVAPELLKrr-eFH 185 human
AAA93318  129 FYFKQLICGLKFIHD-NDVVHRDIKPENLLLTgtHVLKISDFGMATLYRNKG-EERLLDLSCGTIPYAAPELCAgk-kYR 205 Caenorhabditis ele...
Q6DE87    109 KFFQQLIAGVEYLHS-IGITHRDIKPENLLLDerDQLKISDFGLATVFRHNG-KERLLNKMCGTLPYVAPELIKsr-aFH 185 African clawed frog
O61661    122 RYFTQLLSGLNYLHQ-RGIAHRDLKPENLLLDehDNVKISDFGMATMFRCKG-KERLLDKRCGTLPYVAPEVLQk--aYH 197 fruit fly
Q8AYC9    109 RFFQQLIAGVVYLHS-MGITHRDLKPENLLLDerDNLKISDFGLATVFKHNG-RERLLNKMCGTLPYVAPELLRrp-eFR 185 chicken
CAK04818  109 RFFQQLIAGVEYLHS-VGITHRDIKPENILLDdkDNLKISDFGLATMFRHRG-RERALNRLCGTLPYVAPELMSrs-sFN 185 zebrafish
NP_502989 108 GYLKQLMSGLKYIHG-CGIVHRDIKPDNLLINssDALKIADFGLSTRFIVDD-KEILLHERIGTTPFMAPELFAkk-kHR 184 nematode
544017    116 FYFAQLMEGISFMHS-KGVAHRDLKPENILLDynGNLKISDFGFASLFSYKG-KSRLLNSPVGSPPYAAPEITQq---YD 190 fission yeast
585344    120 FYFQQLVSAINYLHVeCGVAHRDIKPENILLDknGNLKLADFGLASQFRRKDgTLRVSMDQRGSPPYMAPEVLYseegYY 199 baker's yeast
P0C198    172 FYFQQLLAGLEYIHS-QGVTHRDIKPENMLLDaeGNLKIADFGLCSVYKYKG-KERELTGACGSLPYIAPEMNGk--pYR 247 Ustilago maydis
Feature 1                                                                                     
1NVS_A    186 AEPVDVWSCGIVLTAMLAGELPWDQPsdsCQEYSDWKe-----KKTYLNPWKKIDSAPLALLHKILVEnPSARITIPDIK 260 human
3JVR_A    185 AEPVDVWSCGIVLTAMLAGELPWDQPsdsCQEYSDWKe-----KKTYLNPWKKIDSAPLALLHKILVEnPSARITIPDIK 259 human
2BR1_A    186 AEPVDVWSCGIVLTAMLAGELPWDQPsdsCQEYSDWKe-----KKTYLNPWKKIDSAPLALLHKILVEnPSARITIPDIK 260 human
AAA93318  206 GPPVDVWSSGIVLIAMLTGELPWDRAsdaSQSYMGWIs----nTSLDERPWKKIDVRALCMLRKIVTDkTDKRATIEQIQ 281 Caenorhabditis ele...
Q6DE87    186 ADPVDVWSCGIVLTAMLAGELPWDQPnevCQEYCDWKe-----KNHYLTPWKKISATPLALLCKMLTEnPQSRITIPDIK 260 African clawed frog
O61661    198 AQPADLWSCGVILVTMLAGELPWDQPstnCTEFTNWRdn---dHWQLQTPWSKLDTLAISLLRKLLATsPGTRLTLEKTL 274 fruit fly
Q8AYC9    186 AEPVDVWACGVVLTAMLAGELPWDQPsdsCQEYSDWKe-----RKTYLAPWRKIDSAPLALLHKILTEnPTARITIPDIK 260 chicken
CAK04818  186 AQPADTWACGIVLTAMLAGELPWDQPsenCQEYLDWLe-----RKTYLTPWKKIDAVPLSLLSKILLHnPEDRFTIPEIK 260 zebrafish
NP_502989 185 GPPVDIWSAGILVFSLLTGNLPWDEPkesDILYSQWMn----nKTSKNCIWYGLSTPVVRFLKKLLKEnVNERATIEEIE 260 nematode
544017    191 GSKVDVWSCGIILFALLLGNTPWDEAisnTGDYLLYKkq---cERPSYHPWNLLSPGAYSIITGMLRSdPFKRYSVKHVV 267 fission yeast
585344    200 ADRTDIWSIGILLFVLLTGQTPWELP---SLENEDFVffiendGNLNWGPWSKIEFTHLNLLRKILQPdPNKRVTLKALK 276 baker's yeast
P0C198    248 GEPVDVWSSGVVLFAMLVGSTPWDEPtsrSPEYSAYRt----gKLFEYDPWPRIPQDALSLLKKMMHPtPEKRITFEGIR 323 Ustilago maydis
Feature 1          
1NVS_A    261 KDRWY 265 human
3JVR_A    260 KDRWY 264 human
2BR1_A    261 KDRWY 265 human
AAA93318  282 ADPWY 286 Caenorhabditis elegans
Q6DE87    261 KDRWF 265 African clawed frog
O61661    275 DHKWC 279 fruit fly
Q8AYC9    261 KDRWY 265 chicken
CAK04818  261 KHRWF 265 zebrafish
NP_502989 261 SDPWL 265 nematode
544017    268 QHPWL 272 fission yeast
585344    277 LHPWV 281 baker's yeast
P0C198    324 RHRWF 328 Ustilago maydis

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