1J7U,2CKQ,1L8T,2PPQ,2CKP,3DXP,3LQ3


Conserved Protein Domain Family
APH_ChoK_like

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cd05120: APH_ChoK_like 
Click on image for an interactive view with Cn3D
Aminoglycoside 3'-phosphotransferase and Choline Kinase family
This family is composed of APH, ChoK, ethanolamine kinase (ETNK), macrolide 2'-phosphotransferase (MPH2'), an unusual homoserine kinase, and uncharacterized proteins with similarity to the N-terminal domain of acyl-CoA dehydrogenase 10 (ACAD10). The members of this family catalyze the transfer of the gamma-phosphoryl group from ATP (or CTP) to small molecule substrates such as aminoglycosides, macrolides, choline, ethanolamine, and homoserine. Phosphorylation of the antibiotics, aminoglycosides and macrolides, leads to their inactivation and to bacterial antibiotic resistance. Phosphorylation of choline, ethanolamine, and homoserine serves as precursors to the synthesis of important biological compounds, such as the major phospholipids, phosphatidylcholine and phosphatidylethanolamine and the amino acids, threonine, methionine, and isoleucine. The APH/ChoK family is part of a larger superfamily that includes the catalytic domains of other kinases, such as the typical serine/threonine/tyrosine protein kinases (PKs), RIO kinases, actin-fragmin kinase (AFK), and phosphoinositide 3-kinase (PI3K).
Statistics
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PSSM-Id: 270690
Aligned: 32 rows
Threshold Bit Score: 76.5733
Created: 26-Sep-2006
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 16 residues -Click on image for an interactive view with Cn3D
Feature 1:ATP binding site [chemical binding site]
Evidence:
  • Structure:1J7U; Enterococcus faecalis APH type IIIa binds the ATP analog, AMP-PNP; defined at 4A contacts.
  • Structure:2CKP; Human ChoK A-2 binds ADP; defined at 4A contacts.
  • Structure:3LQ3: Human ChoK Beta binds MgADP; contacts at 4A.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1            #   #    #            # #                                         #        
1J7U_B       17 YRCVKDTEGMsPAKVYKLVGen--eNLYLKMTDsrykg-------ttydvEREKDMMLWLegk-----lpVPKVLHFErh 82  Enterococcus fae...
2PPQ_A       25 TSYKGIAEGV-ENSNFLLHTtk--dPLILTLYEkrve---------kndlPFFLGLXQHLaak----glsCPLPLPRKdg 88  Agrobacterium tu...
3DXP_A       41 LSVEQFKGGQ-SNPTFKLVTpg--qTYVXRAKPgpkskll----psahaiEREYRVXDALagt----dvpVAKXYALCed 109 Ralstonia eutrop...
AAW34150     16 DSIKLIGEGY-DSKAYIVNN-----EYVFKIKFsank---------kkgyEKEKAIYDFLnkkln-tnikIPNIEYSYis 79  Campylobacter je...
AAX82584    135 DSIEIIGSGY-DSVAYLVNN-----EYIFKTKFstnk---------kkgyAKEKAIYNFLntnle-tnvkIPNIEYSYis 198 Enterococcus fae...
AAW34147      4 ISRKYINEGF-NSKAYIIND-----DYILLDGVnqns---------yknyQKYVNVINQIsnvk---sleIPRIIELIep 65  Campylobacter je...
NP_906885    16 HEGFQLTEWGgGNLNYLYRVdstkeSYILKYAPpyirclgegyplsserlRYEILGLKAAneti---pefAPKLFYVAk- 91  Wolinella succin...
YP_382396    45 VEPSRLTGGA-DARLYRYKLid-qePRVLRILRpa-------------reVAELLHHQVVhqilnqqglnAPMIHHVCad 109 Synechococcus sp...
ZP_00371007  22 IRLFPIKEGQ-ESQAYWFSRgg--rEYVVRINSnme------------gfKKDKYAYEHFrsd----rvpIPEVVETGnf 82  Campylobacter co...
AAB03644     24 HGPLTVNELGlDYRIVIATVdd-grRWVLRIPRraev---------sakvEPEARVLAMLkn-------rLPFAVPDWrv 86  Escherichia coli
Feature 1                    ####                                                               
1J7U_B       83 d------gwSNLLMSEADGvlcseey--------edeqspEKIIELYAECIRLFHSIDIsdcpytnsldsrlaeldylln 148 Enterococcus fae...
2PPQ_A       89 ellgelsgrPAALISFLEGxwlr--------------kpeAKHCREVGKALAAXHLASEgfeikrpnalsvdgwkvlwdk 154 Agrobacterium tu...
3DXP_A      110 es---vigrAFYIXEFVSGrvlwdqsl-----pgxspaerTAIYDEXNRVIAAXHTVDYqaiglgdygkpgnyfqrqier 181 Ralstonia eutrop...
AAW34150     80 e------elSILGYKEIKGtfltpeiy-----falskekqELLKQDIAMFLRQMHDLDYseissytidnkqnvleeyqll 148 Campylobacter je...
AAX82584    199 d------elSILGYKEIKGtfltpeiy-----stmseeeqNLLKRDIASFLRQMHGLDYtdisectidnkqnvleeyill 267 Enterococcus fae...
AAW34147     66 ce---eypnGALIYKMIKGhtfrkeh--------ieivnlDNIAKKLAEFMDELYEIRVdfdkdeyikneleiteqsvie 134 Campylobacter je...
NP_906885    92 ---------EGVVMEYLEGfkllrqa-------fiqgekiPHLAEHLAEMLARFLVKNSfwalnegerkklleqsenslm 155 Wolinella succin...
YP_382396   110 ks---vlggVFAVMELVPGqtvf-------------gmapELHAKAVGESMATMHELDVrpivesfrragvpderfldpf 173 Synechococcus sp...
ZP_00371007  83 d-----gthYFCISVKADGityeds----------deetvVRLLGDITDVTEAISRTDIsgtsgcgvfdsdtgnapfysw 147 Campylobacter co...
AAB03644     87 a------naELVAYPMLEDstamviqpgsstpdwvvpqdsEVFAESFATALAALHAVPIsaavdagmlirtptqarqkva 160 Escherichia coli
Feature 1                                                                  #   ## #           ##
1J7U_B      149 ndladvd------------------cenweedtpfkdprelydflktekpeeeLVFSHGDLGDSNIFVkd--gkVSGFID 208 Enterococcus fae...
2PPQ_A      155 seerad---------------------evekglreeirpeidylaahwpkdlpAGVIHADLFQDNVFFlg--deLSGLID 211 Agrobacterium tu...
3DXP_A      182 wtkqykl-------------------setesipaxdslxdwlpqhipqedadlTSIVHGDYRLDNLXFhpteprVLAVLD 242 Ralstonia eutrop...
AAW34150    149 ketiyd---------------------sltdiekqyveefmqrlnsttifdgkKCLCHNDFSCNHLLLdde-nrLCGVID 206 Campylobacter je...
AAX82584    268 retiyn---------------------dltdiekdyiesfmerlnattvfegkKCLCHNDFSCNHLLLdgn-nrLTGIID 325 Enterococcus fae...
AAW34147    135 lkey------------------------lsesnyekilswfneyknylltfndYHFIHGDLWYENYILndn-neLVGIVD 189 Campylobacter je...
NP_906885   156 rrlsedyiftfpfmehptnqknptsapsvreihqdkewlgailwlksrfstakEGLLHGDLHTGSLMIkg---eQIKMID 232 Wolinella succin...
YP_382396   174 vrqkal--------------------gffeektpwaadlmawlrdhlpldaenLAVIHGDYHGGNLMFkn--gsLSGVLD 231 Synechococcus sp...
ZP_00371007 148 reylaevferd-----------wtavsrsyvnlslidellaayrelisycpeeRALFHGDFGSNNVIVgkk-srISGVID 215 Campylobacter co...
AAB03644    161 ddvdrv--------------------rrqfvvndkrlhrwqrwldddsswpdfSVVVHGDLYVGHVLIdnt-erVSGMID 219 Escherichia coli
Feature 1                             
1J7U_B      209 LGRSGRadKWYDIAFCVRSIRE 230 Enterococcus faecalis
2PPQ_A      212 FYFACNdlLAYDVSICLNAWCF 233 Agrobacterium tumefaciens
3DXP_A      243 WELSTLghPXGDFGYHCXSWHI 264 Ralstonia eutropha JMP134
AAW34150    207 FGDSGIidEYCDFIYLLEDSEE 228 Campylobacter jejuni
AAX82584    326 FGDSGIidEYCDFIYLLEDSEE 347 Enterococcus faecalis
AAW34147    190 FEGSGMgdPAYDIAALYYLGTG 211 Campylobacter jejuni
NP_906885   233 FEFSFVgpLSFDLGAILANWLI 254 Wolinella succinogenes DSM 1740
YP_382396   232 WSFCISd-PAVDLAHTMNDYLV 252 Synechococcus sp. CC9605
ZP_00371007 216 WDCAAYgdFLYDIATAYFWRTW 237 Campylobacter coli RM2228
AAB03644    220 WSEARVddPAIDMAAHLMVFGE 241 Escherichia coli

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