2VKN


Conserved Protein Domain Family
SH3_Sho1p
?
cd11855: SH3_Sho1p 
Click on image for an interactive view with Cn3D
Src homology 3 domain of High osmolarity signaling protein Sho1p
Sho1p (or Sho1), also called SSU81 (Suppressor of SUA8-1 mutation), is a yeast membrane protein that regulates adaptation to high salt conditions by activating the HOG (high-osmolarity glycerol) pathway. High salt concentrations lead to the localization to the membrane of the MAPKK Pbs2, which is then activated by the MAPKK Ste11 and in turn, activates the MAPK Hog1. Pbs2 is localized to the membrane though the interaction of its PxxP motif with the SH3 domain of Sho1p. SH3 domains are protein interaction domains that bind to proline-rich ligands with moderate affinity and selectivity. They play versatile and diverse roles in the cell including the regulation of enzymes, changing the subcellular localization of signaling pathway components, and mediating the formation of multiprotein complex assemblies.
Links
?
Statistics
?
PSSM-Id: 212789
Aligned: 9 rows
Threshold Bit Score: 83.6236
Created: 1-Jun-2011
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
Download Cn3D
 
peptide ligand
Conserved site includes 9 residues -Click on image for an interactive view with Cn3D
Feature 1:peptide ligand binding site [polypeptide binding site]
Evidence:
  • Structure:2VKN; Saccharomyces cerevisiae Sho1 SH3 domain binds Pbs2 peptide; contacts at 4A.
  • Comment:SH3 domains bind proline-rich ligands, preferentially to PxxP motifs.
  • Comment:flanking hinge and loops (RT and n-Src) confer sequence specificity for ligand residues outside the core binding motif
Download Cn3D for Viewing 3D StructureScroll to Sequence Alignment Display
Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection: 
Feature 1             # #        #                ##             ####   
2VKN_A         7 KAKALYPYDADDDDayEISFEQNEILQVSDIeGRWWKARRAnGETGIIPSNYVQL 61  baker's yeast
CBQ73560     288 KARALYAYQANADDptEISFSKGEVLDIVDNsGKWWQARKSnGETGIVPSNYMQL 342 Sporisorium reilianum
BAE62426     234 RAKAIYSYDANPEDanEISFTKHEILEVSDVsGRWWQARKSnGDTGIAPSNYLIL 288 Aspergillus oryzae
NP_001168872 249 RAKAIYSYDANPEDanEISFAKHEILEVSDVsGRWWQARKQnGETGIAPSNYLIL 303 maize
XP_002489490 217 TVRALYNYDANPEDinELSLKQGEVFKVKDTaGKWWQAKKQsGELGICPSNYVET 271 Pichia pastoris GS115
EFW95233     226 TVRGLFDYDASPDDinELSFKKGDIFRVKDTvGNWWQGKNSkGEIGMCPSNYLEV 280 Pichia angusta DL-1
731506       304 KAKALYPYDADDDDayEISFEQNEILQVSDIeGRWWKARRAnGETGIIPSNYVQL 358 baker's yeast
EGG11682     337 PARALFSYKASPADprELSIEKGEVVEIHDShGKWWRAQKKdGTSGILPSNYVAL 391 Melampsora larici-populina 98AG31
XP_001885488 302 RAEAMFPYTASPKDpnELSFGRGEILNIIDEsGKWWEAEMDdGRKGIVPSNYLRL 356 Laccaria bicolor S238N-H82

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap