Conserved Protein Domain Family
CE4_PuuE_HpPgdA_like_2
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cd10941: CE4_PuuE_HpPgdA_like_2 
Putative catalytic domain of uncharacterized prokaryotic polysaccharide deacetylases similar to bacterial PuuE allantoinases and Helicobacter pylori peptidoglycan deacetylase (HpPgdA)
This family contains many uncharacterized prokaryotic polysaccharide deacetylases (DCAs) that show high sequence similarity to the catalytic domain of bacterial PuuE allantoinases and Helicobacter pylori peptidoglycan deacetylase (HpPgdA). PuuE allantoinase appears to be metal-independent and specifically catalyzes the hydrolysis of (S)-allantoin into allantoic acid. Different from PuuE allantoinase, HpPgdA has the ability to bind a metal ion at the active site and is responsible for a peptidoglycan modification that counteracts the host immune response. Both PuuE allantoinase and HpPgdA function as homotetramers. The monomer is composed of a 7-stranded barrel with detectable sequence similarity to the 6-stranded barrel NodB homology domain of DCA-like proteins in the CE4 superfamily, which removes N-linked or O-linked acetyl groups from cell wall polysaccharides. In contrast to typical NodB-like DCAs, PuuE allantoinase and HpPgdA do not exhibit a solvent-accessible polysaccharide binding groove and might only bind a small molecule at the active site.
Links
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Statistics
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PSSM-Id: 200566
Aligned: 40 rows
Threshold Bit Score: 194.048
Created: 10-Aug-2011
Updated: 2-Oct-2020
Structure
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Aligned Rows:
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putative activeputative
Feature 1:putative active site [active site]
Evidence:
  • Comment:Based on similarity to the sequence of Pseudomonas fluorescens PuuE allantoinase
Scroll to Sequence Alignment Display
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                            # #                                                                       
NP_841828                    6 RNALTIDVEDYFqvsafaryipr------------sswdslpcrveRNIDRILVLLDEHKIKATFFTLGwIAERYPSMVK 73  N...
EFL50887                     6 ALAVTVDMDEWYhcrwatgsprsrwrtteaffrdrygadcpageirEPMARVLALFTRHGVRATFFILGeMAQAYPDLVR 85  D...
AEG91979                     6 SAIFTLDLEDHVgrydt------------------------ssrypSISRQVLQTLAQNGIVGTVFVVGkIAKAEPALLR 61  R...
NP_616012                  275 KMSITVDVEDWYhipsvcgssfsvykdv---neffkkwndrydylsEPTRKTLDLLDEFNITATFFIVAdVVKRYPGLIE 351 M...
ZP_03130876                  3 TFLFSIDLEEFYaeraef----------------------qrtplpELVERYLALLRRREMKATFFIVGeIAQKFPSVIR 60  C...
hbprmlniaid:GbCGDNIH1_0038  26 PVTFSVDLEDHGggld---------------------------rlaGNASRLLDWLASNHVRATIFTVGeLGRTAPAIIR 78  G...
ADI22504                     3 IAVLSMDIEDWYhldylegsk-----------------cnkrysllDGIDVYLEILQKFNIQSTFFVLGeIAESINPTLK 65  u...
EFK33078                     4 YAVLSMDVEEWYhldyftkee-----------------celnqstlDGLDIYLDILDQYDIKTTFFIVGeLVEVLKDKIK 66  C...
YP_003815998                 2 ALFLSSDVEQDAppymnt-----------------------ergivEGLPRLLELLDELKVRATFFVVGrVAERHPRAVY 58  A...
CBE68127                    58 SLIFTCDLELDPpweagswd------------------trtlrgieEGLPSLLNLLDAHGVRGTFFTEGiLARLAGDTVL 119 N...

Feature 1                                 #   #                                                ###             
NP_841828                   74 RIVEnGHELASHGYAHHrvte--------------lsrGQFYDDIARSKFLLEDigGQSVWGYRAPSFSinkdnLWALDY 139 N...
EFL50887                    86 DVVAaGHEVACHGMHHVdlg----------------erQAFTDDLRRAKGILEDvsGQAVTGFRAPNLIl---rPWVLDI 146 D...
AEG91979                    62 EISDlGHELACHSLDHTpldk--------------qapAAFREDTARAKHLIENciGKAVVGFRAPVFSltkstLWAVDV 127 R...
NP_616012                  352 SISEkGHEIACHGADHRcvvdtkt-------keplidvEEFEVTTLNAKKLLERtsGQEVIGYRAPNGAv---tGWMLDS 421 M...
ZP_03130876                 61 ALAAeGHELACHTFDHIplne--------------rnpDTLRDDLRRNLDALAEfaTTPVQGFRAPILSlgekqQWAYEI 126 C...
hbprmlniaid:GbCGDNIH1_0038  79 RFAEaGHEIACHGEVHErleq--------------lspAQLRTGLIAARQRLSDlsGQSVEGFRAPEFSltpltPWVPNV 144 G...
ADI22504                    66 QIIKeKHEIASHGSNHVrpit--------------iteSEFYNDLHKSKHTLEHiiGASIEGYRAPCFSi---dRKRLDQ 128 u...
EFK33078                    67 YIKAkGHEICLHSYAHKrplq--------------lslDGFREDTVKGIDSFREftGLEVDGYRAPCFSl---dRPRLEI 129 C...
YP_003815998                59 NIIErGHELGSHGYAHVrldr--------------vplREAEDDVRRSLRVLRD--FSEVKSFRAPNLKl---pRQLLPV 119 A...
CBE68127                   120 EVARrGHEIGCHGLAHEsyggpyriddtiprpptvygrTEKEDTVRKAKEMLEDlvGVPIRAFRAPFLHi---dSQSAAA 196 N...

Feature 1                                                                                                      
NP_841828                  140 LEEAGYRYSSSIYPVEhdhygmpdap-----------rfafnptgsrKILELPVTTvr------lLDRNFPAggggyfrl 202 N...
EFL50887                   147 LHDLGFAYDSSVCPSRalfgkyeglseapqt-pyrpaagdparrgghPVVEIPIPAf--------PVLKLPAatgimtrv 217 D...
AEG91979                   128 LAELGFQYSSSVMPTHnplygfpqa-------------ptkpflwknGLVEIPCPVgk------lGPITLPYlggfylry 188 R...
NP_616012                  422 LEKIGFKYDSSVSVNSlynksdsslrhvssfpyypapgslepssekrNFVEFPWAYy-------dIGIKIPTyggptlrf 494 M...
ZP_03130876                127 LAELGFTYSSSVLPAKnplhgwpgf--------------glqprrlhGVLEVPVTLsp------fFGLEVPVgagtyfrc 186 C...
hbprmlniaid:GbCGDNIH1_0038 145 LAEAGYLWSSSVMPTRlrggkapigwa---------glparpflwpsGVLEIPVPLlr------iGPFHVPFmggmwlry 209 G...
ADI22504                   129 VRKAGFSYDSSRMDFSehplyetinmdg-------fnvvmpnifrlnNFFEFEASThp------vYGKNVPIsgggylrl 195 u...
EFK33078                   130 LRELGFSFDSSKIKFDahdlygsinldd-------feefhptiykkeSFYEFEASTve------vLGKNLPVsgggylri 196 C...
YP_003815998               120 LREEGVSVDASLASYKppfprg--------------------ltvegGVVRVPATVt-------sSVLRVPWa------- 165 A...
CBE68127                   197 VTNAGFLVDSSLPNQLfgylstpahrnpg-----rpltrlrdtlpqhRLIEIPVSVeprpslrshHPYRFIQqtt----- 266 N...

Feature 1                                                #                                                     
NP_841828                  203 wpYAVSRWFLQRvnsv-drqPAIFYFHPWEIDpdqp-------rqvGISFKTRFRHYLnl-----gRMEKRLDALIRHFD 269 N...
EFL50887                   218 agSWWCRIGLGRalr---kgAACYYFHPYEIApapk-------fpgKPLKVRLFLRRTg------pWLERTLDRLLRDLN 281 D...
AEG91979                   189 lpQILVRSLITQadp---esCLWTYCHPYDFDtaepf-----nriqGAPLWTSILLWFnr-----rDTLPKILAVVRENS 255 R...
NP_616012                  495 lgSHLKFRGLKQsln---rgDTIFYFHPIDISrekf-------psiGNRRPFYWTIKGk-------IVEERLRYVFKEIE 557 M...
ZP_03130876                187 lpFGSIQRRFTDhae--adtAIVGYFHPYDIDtaqerv--msrgvgGSRLLNTLLYFNr------gGTLRRLESILDAGF 256 C...
hbprmlniaid:GbCGDNIH1_0038 210 lpLPLLRLAVKRmg----gqPSWAYCHPYDIDtk----------ekFSLLPGRSCVASmamwanrgRMVERMTILAANPA 275 G...
ADI22504                   196 lpWYISETLIKRyik--snnYYMLYIHPFELSikp---------npPFPSPTKWHNRLrfgi-grsTVAEKLKRLIELLK 263 u...
EFK33078                   197 fpWALTKMLLKKyfk--knqHYFFYIHPFEFSkny---------niTVPENTDFKTKIrfnq-grkSVENKMHKLIQLLK 264 C...
YP_003815998               166 -lQRPLLALMPR--------VTTLFVHPWEVSgvvhwrp-diwvgtGPRLLGLLRNAVs-------YMVRRGYRPMTMSE 228 A...
CBE68127                   267 rtQSVLQDLIAVsalasvpiTLVLLVHPWEFVegfpspfghdrgsvRTERLARFLDTVs-------AVAPVRCATIGAFA 339 N...

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