1P4V


Conserved Protein Domain Family
Glycosylasparaginase

?
cd04513: Glycosylasparaginase 
Click on image for an interactive view with Cn3D
Glycosylasparaginase and similar proteins
Glycosylasparaginase catalyzes the hydrolysis of the glycosylamide bond of asparagine-linked glycoproteins. This enzyme is an amidase located inside lysosomes. Mutation of this gene in humans causes a genetic disorder known as aspartylglycosaminuria (AGU). The glycosylasparaginase precursor undergoes autoproteolysis through an N-O or N-S acyl rearrangement of the peptide bond, which leads to the cleavage of a peptide bond between an Asp and a Thr. This proteolysis step generates an exposed N-terminal catalytic threonine and activates the enzyme.
Statistics
?
PSSM-Id: 271335
Aligned: 89 rows
Threshold Bit Score: 291.002
Created: 2-Nov-2006
Updated: 2-Oct-2020
Structure
?
Program:
Drawing:
Aligned Rows:
 
Conserved site includes 7 residues -Click on image for an interactive view with Cn3D
Feature 1: active site [active site], 7 residue positions
Conserved feature residue pattern:x x [NDG] T [TS] R [TS]Click to see conserved feature residue pattern help
Evidence:
  • Structure:1P4V: Elizabethkingia meningoseptica glycosylasparaginase precursor mutant binds the inhibitor glycine; contacts at 4.0A

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1             #                                                                          
1P4V_A         6 IVLSTWnfGLHANVEAWKVLSkg-----gkALDAVEKGVRLVEDDPt--eRSVGYGGRPDRdGRVTLDACIMDEn-YNIG 77  Chryseobacteriu...
WP_003148228   3 GIIATWrmALEGVSAASKMLAkk-----eqSGDAVEMAIREVEDFPy--yKSVGFGGLPNAeGDVELDAGFMDGdtLDFG 75  Atopobium rimae
EUM15143       3 GIIATWrmALEGVTESASALAag-----kpVATAVVDAVAAVEDFPf--yKSVGYGGLPTEnGEVELDAAFMDGdtLAFG 75  Enterobacter sp...
XP_005537331   8 LVVATWefSQSGVESCFDCLRkg-----gkPLDAVETGIHVVELEPt--vTSVGYGGLPNAnGELELDAAITDG--AHFG 78  Cyanidioschyzon...
WP_006584071  10 VIGGTWrmCLEGISLGGRILAeg-----gsAGDAVCEAVKVVEDEPr--yKSVGFGGLPNGdCQVELDAAFMDGdtLDFG 82  Thermanaerovibr...
YP_004525613   4 AVIGTWkmAHDGVCLASEILEke-----gsLAEAIKRAINDVENNPe--yLSVGYGGLPNReGEVELDAAYMDGstGLYG 76  Treponema azoto...
XP_006813865   3 ACMVTWn-FLQAVESSSKSLLlg-----ddYITALERGINVVELDNs--vKSVGLSGIPNShGELQLDAAIMKGdmLQFG 74  Saccoglossus ko...
ESO86677       9 RIVGTWpfSFEAVKHASTVIQkskg--dnvCIDAVETGINKIESNSdygiYFVGCGGIRNKnGFLELDAAIMHGrnLDYG 86  owl limpet
XP_005708574   1 MIATWDf-GLQAVENASSILGkhentgneaLLDAIVRGIESVELDPk--vSSVGYGGLPTAeGILQLDAAVMLGn-GKGG 76  Galdieria sulph...
ELT91383       3 ASVATWsfGLKAVQKSKENLCsg-----klSLDATEYALARVENDVstgvYIVGRGGFPNAdGFLECDAALMDGnsLKFG 77  Capitella teleta
Feature 1                                                                                       #
1P4V_A        78 SVACMehIKNPISVARAVMektpHVMLVGDGALEFALSQGFKKENLLTAe---SEKEWKEWLKTSQykpi----vnieNH 150 Chryseobacteriu...
WP_003148228  76 SVGALkdFAHPVSIARALShdgvNCVLVGEGAEKYAARQGFARKTMLTDr---AKIHYMNRLKADGglphqedlkpyaGH 152 Atopobium rimae
EUM15143      76 AVGNLvdIANPVRVAHALSrqryNSLLVGQGAREWALSQGFADKSMLTEr---AMQHYRKRCRETLdkgl----spydGH 148 Enterobacter sp...
XP_005537331  79 AVFALrgYRSAIGIARGVLerseHTALAGNGAVNFAQEVLGEAPLPDSEl---LTEHARRRLAEYRagrfq--grpneSH 153 Cyanidioschyzon...
WP_006584071  83 AVAAVrdVKNPVMLARRLSserfNTFLVGQGAQDFARVELFEMRNMLTPr---AKRAHEIRRRRMLeenl----spydGH 155 Thermanaerovibr...
YP_004525613  77 SVIGAkgIANPIDAAIELGkrqlNCMLSGEGAEKYARMNGFEIKNMLTGn---AKKRWLEMRESAKdsv-------yqGH 146 Treponema azoto...
XP_006813865  75 GVAAMkrIDRPISVARKVL----ECSHHSLIVGEGATQFAVEKGFKLQPl---ITEKQVPVDSSSP------------CH 135 Saccoglossus ko...
ESO86677      87 AVTAIqgINKPISVARKVM----DKSPHSILTAQGAQQFAVKHGFDLNH----DLMIQKLDPNNSKp----------rGH 148 owl limpet
XP_005708574  77 SVLSLqgFPAAIPIARLVLeksrHPCLCGYGAQKLAEQYGFRSVDNANLltpyARQRWEQFQKQSDqas-------ndLC 149 Galdieria sulph...
ELT91383      78 AVMALrgIATPSRVARRVLenspHSILVGEGAQQFALSQGFTVEKEEDLn---ASSSEFVMTSGHS------------DT 142 Capitella teleta
Feature 1        ##                      #         #                      #                      
1P4V_A       151 NTIGMIALDa-----qGNLSGACTTSGMAYKMHGRVGDSPIIGAGLFVDNeIGAATATGHGEEVIRtVGTHLVVELMNQG 225 Chryseobacteriu...
WP_003148228 153 DTVGMVCLDe-----kGSITSATSTSGLFMKRPGRIGDSPVCGAGLYADShYGGATVTGLGEDCMRgCISYEVVRLMGEG 227 Atopobium rimae
EUM15143     149 DTVGVIGLDk-----qGSMSVATSTSGLFMKKRGRIGDSPIIGSGFYCDSeTGAATATGVGEDLMKgCTSYEIVRRMAQG 223 Enterobacter sp...
XP_005537331 154 DTVGMIAAQs-----tGDIAAGCATSGYPFKRPGRVGDSPLPGGGLFCESgLGAAVATGDGDDLMRlCPTHLIVELMRNG 228 Cyanidioschyzon...
WP_006584071 156 DTVGVVALDl-----rGSMACATSTSGLFMKRPGRVGDSPLCGSGLYCDSrMGAAVATGLGEDIMKgALSYETVRLMGEG 230 Thermanaerovibr...
YP_004525613 147 DTVGVIVCRe------RCIAAGVSTSGLFYKEPGRVGDSPIIGGGLYCLNgIGGAAATGVGEDILRgCLSYEAVRQMESG 220 Treponema azoto...
XP_006813865 136 DTVCMIILDr-----hGNIAVGSSTSGSPNKHVGRVGDSPLPGCGLYADNqIGAAAATGNGDEIMRfCCTFQIVQYMKQG 210 Saccoglossus ko...
ESO86677     149 DTLGLICYLn------GEFVVGVSTSGMGGKEAGRVGDSAICGAGLYADNeGGAACCSGDGDLILKhCPAYRVVSYLRMG 222 owl limpet
XP_005708574 150 DTVGLICCTyddvtkeSVIGAGCATSGAAFKQDGRVGDSAIFGCGLYAQNgIGAATSTGDGDVIMKyCCAYQIVEEMRHG 229 Galdieria sulph...
ELT91383     143 LGVINLDAS-------GNIVAGVSTSGMPGKHPGRVGDSPLPGGGLYADDrIGAASCTGDGDQILPfCPSLKAVLKMGQG 215 Capitella teleta
Feature 1                                                                   
1P4V_A       226 rTPQQACKEAVERIVKIvnrrgknlkdIQVGFIALNK-KGEYGAYCIq--dGFNFAVHD 281 Chryseobacterium meningosepticum
WP_003148228 228 lSPQEACERAVFELDRKlksm--rgsaGDLSVVAMNA-QGLWGVATNt--dDFSFVVAT 281 Atopobium rimae
EUM15143     224 mTPQQAADSVVFELEDKlmsr--fgrvGDLSVVCMNN-RGEFGAATNi--kTFSFVVAT 277 Enterobacter sp. BIDMC 29
XP_005537331 229 qSPQRACEEAVQRVMRYreqt---grdCRGAFIAVRCdALAWGAHATh--eGFVVAHAD 282 Cyanidioschyzon merolae strain 10D
WP_006584071 231 fSADRAARRALREFEARvlsr--grkvGAISIICCSP-RGDIGVATNv---EFSFAWGS 283 Thermanaerovibrio velox
YP_004525613 221 kSAQQACDDALAAHIKRmdgl--giklGDISLVALDA-GGGLGAATNm--qEFPFVYAD 274 Treponema azotonutricium ZAS-9
XP_006813865 211 lDVQSACQQMVTNISERigv----nniFEVGFIAMDK-QGNCGAAGTv--kSWSDNATE 262 Saccoglossus kowalevskii
ESO86677     223 wKSEDACKKVITEIMIRsk------dfIELVIIAVDK-EVFYPKIILkktrIIALCIYQ 274 owl limpet
XP_005708574 230 lGPQRACEVVVARLRSQvp-------eCQAAFVAMNC-AGEYGAYSTh--qGFSYAIWN 278 Galdieria sulphuraria
ELT91383     216 mTPNAACESVIKEIAERifp----kemFEIGIVALSV-EGEYGAAGSl--kRWRDIITG 267 Capitella teleta

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap