2D1Q,2D1S,4G36,3A9V,3NI2


Conserved Protein Domain Family
Firefly_Luc_like

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cd05911: Firefly_Luc_like 
Click on image for an interactive view with Cn3D
Firefly luciferase of light emitting insects and 4-Coumarate-CoA Ligase (4CL)
This family contains insect firefly luciferases that share significant sequence similarity to plant 4-coumarate:coenzyme A ligases, despite their functional diversity. Luciferase catalyzes the production of light in the presence of MgATP, molecular oxygen, and luciferin. In the first step, luciferin is activated by acylation of its carboxylate group with ATP, resulting in an enzyme-bound luciferyl adenylate. In the second step, luciferyl adenylate reacts with molecular oxygen, producing an enzyme-bound excited state product (Luc=O*) and releasing AMP. This excited-state product then decays to the ground state (Luc=O), emitting a quantum of visible light.
Statistics
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PSSM-Id: 341237
Aligned: 39 rows
Threshold Bit Score: 480.942
Created: 20-Jun-2007
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
AMP bindingacyl-activating
Conserved site includes 16 residues -Click on image for an interactive view with Cn3D
Feature 1:AMP binding site [chemical binding site]
Evidence:
  • Structure:3A9V; Populus tomentosa 4-coumarate-CoA ligase binds AMP, contacts at 4A.
  • Structure:2D1Q; Luciola cruciata luciferase binds AMP, contacts at 4A.
  • Citation:PMID 8805533
  • Structure:2D1S; Luciola cruciata luciferase binds 50-O-[N-(dehydroluciferyl)-sulfamoyl adenosine (DLSA), contacts at 4A.
  • Comment:luciferyl adenylate releases AMP

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                     
2D1Q_A     43 AFTNAvtgvDYSYAEYLEKSXXLGKALQnygl-------vvdGRIALCSENCEEFFIPVIAGLFIGVGVAPTNEIYtlRE 115 Japanese firefly
Q5BA81     42 PFVCAitgkSYSTKVLVDRVEFLSRSLAqelgwspnegepedKVVGIYSWNTLDFFALCWAVHRLNGICLPLHPFSivPE 121 Aspergillus nidula...
3A9V_A     40 CLINGangdVYTYADVELTARRVASGLNkigi-------qqgDVIMLFLPSSPEFVLAFLGASHRGAIITAANPFStpAE 112 Populus tomentosa
NP_647992  55 AQISVtentELTWKDIQTNAMKVASYMRklgl-------eqgDFVGVIGRLTTHLTALAYACFFNGTPYHALHTEYeqSA 127 fruit fly
NP_650831  47 AQISAtentILTRAELQANAMHIASYMRslgl-------lqmDIVGIIARNTTHISAVAYACFFNGIAFHSLNISYeqST 119 fruit fly
NP_650160  46 CQISDsegvEVTFGQALTWAIRIAQQLKsrgl-------dhkDIIGISARNTTYIMPTAVACFFHGTPFQSANPILeeST 118 fruit fly
NP_650832  46 CQISDtegtALTNGEAITFAIRIAQQLKamgl-------kqdDVVGIVGTNTTYLMPVVLGCLLNGTPFHAVSPWQdeDT 118 fruit fly
NP_610779  49 CDVDGv---TVTFEQGLTWSIRIAQYLKkrgl-------nhkDVIGIAAKNSTYVMPLGVACLMNGTPFHSVNPVLddAT 118 fruit fly
NP_650830  53 SHTDNt---RLTRFQMLQNAAKIGCYLRdqgf------kketDLVGLMARNSTHVGALAYGCLFNGTPFHAVNPNLehNT 123 fruit fly
NP_611913  50 QITDDdg-tVLTNADMLAYATRIALFFKsegl-------tqeDRVGIIANSSTFVIPVATACFFQATPFHAVNYSRepAI 121 fruit fly
Feature 1                                                                                     
2D1Q_A    116 LVHSLGISKPTIVFSSKkgLDKVITVQKtvt-tikTIVILDskvdyrgyqcldtfikrnt------ppgfqassfktvev 188 Japanese firefly
Q5BA81    122 VVAHMKRAKCRVIFTCQslVANTLEAARelsipgdKIYTTAlpeaylqnpepidqfksvdqliaegeklqrlpplqwekg 201 Aspergillus nidula...
3A9V_A    113 LAKHAKASRAKLLITQAcyYEKVKDFARes---dvKVMCVDsapdgclhfseltq---------------adeneapqvd 174 Populus tomentosa
NP_647992 128 IERLFGITKPRLIFCDGdeFEKVQAATKgl---qvQIVTMRnhpagilriqdilttp------------vemnfrpvrlk 192 fruit fly
NP_650831 120 IEKLFSITRPNIIFCDGdeFEKVRSATAql---dvKIITMRnhpsgsiridqvlstp------------ieknfqpvrle 184 fruit fly
NP_650160 119 LKHLYNISKPKIIFTDAdhYDKLYSATSaf---kpEIILTTgtkdgvlsiqdllapt-----------kteffyqptplk 184 fruit fly
NP_650832 119 IKHLFSITRPKLIFCDGkcFQRLSIIARil---ksHVYTLKdhrlgmprvedllept-----------taelyyvpetll 184 fruit fly
NP_610779 119 LTHVFSITKPTLIFCDGqeYDKVHKATVgw---hpEILTLTdhvegvqgietlldpt-----------ttekiyqpevlk 184 fruit fly
NP_650830 124 ISSLYKITRPRILCCDTadYEKIKDIGAsl---gaLIITVNgklpgvisvadilqnp------------lpddyepaqfq 188 fruit fly
NP_611913 122 VQGLYSVTKPKIMFIDGpdYDRIKEITKew---spKLITLTgkvegvtsiedlvkph-----------paekiyvpasla 187 fruit fly
Feature 1                                                                #                    
2D1Q_A    189 drkeQVALIMNSSGSTGLPKGVQLTHENIVTRFSHARDpiygn-qvspgTAVLTVVPFHHGFGMFTTLGYLI-CGFRVVM 266 Japanese firefly
Q5BA81    202 rakiQVAYYCATSGTSGKQKLAKITHYNFIANVMQVCMhesya-kngrnEIAFGAIPLTHGYGLNIGHIMVY-RGDTYVI 279 Aspergillus nidula...
3A9V_A    175 ispdDVVALPYSSGTTGLPKGVMLTHKGLITSVAQQVDgdnpnlyfhseDVILCVLPMFHIYALNSIMLCGLrVGAPILI 254 Populus tomentosa
NP_647992 193 dgtdQLLAILSSSGTSGLPKAVTISNSHQIIGSFLPVDs---------sIIQYNPNTLDWASGITMTINAAV-FSLTSII 262 fruit fly
NP_650831 185 qgndQTLAILCSSGTTGIPKAVTITNSRQILNSSHSLTt---------nDVQYSHSTLDWITGLLTTVTSGVfSTKRIIA 255 fruit fly
NP_650160 185 egpsQTVAILTSSGTTGMPKAVCISNDILTQETVFVNGy----------DTIFISASLDWITGLWATIFSTV-NGCTRII 253 fruit fly
NP_650832 185 lggdHTVAILCTSGTTGLPKAVCISNSACLFDFGFVTGq----------DVLLSFSTIDWSAGMFNMLFSCC-HGSTRII 253 fruit fly
NP_610779 185 eggdQTVAILCSSGTTGLPKAVCISNSILIQDSMLITSq----------SVIYVGSCLDWITGLWAFVFSTV-FGCTRII 253 fruit fly
NP_650830 189 rgvdRTMAILCSSGTTGTPKAVTLSNSRKLFEMHSYLGs---------dDVQYAPSTLDWLTGLITLVTAAV-FGTVRLI 258 fruit fly
NP_611913 188 tggdQIAVVLCSSGTAGLPKAVALSHRHIASTNSLCISt----------DVLYTSATIDWMTGFSITVMNLT-CGFTRII 256 fruit fly
Feature 1                                                          ###                        
2D1Q_A    267 LTKFde-etFLKTLQDYKCTSVILVPTLFAILNKSELln-kyDLSNLVEIASGGAPL----SKEVGEAVArrfnlpGVRQ 340 Japanese firefly
Q5BA81    280 CPRFdm-qlMLKTIERFRVERLYVVPPILAALAANPFlldlhDLSSVQATVTGAAAL----DRSIAAKLNklrptwKINH 354 Aspergillus nidula...
3A9V_A    255 MPKFei-gsLLGLIEKYKVSIAPVVPPVMMSIAKSPDld-khDLSSLRMIKSGGAPL----GKELEDTVRakfpqaRLGQ 328 Populus tomentosa
NP_647992 263 EDCDfdpanLCGLIEKYRISMVFVSSSQLAMLSNCPEfy-aaDLSSVKYFFYGGSNC----SLEVQNKIRsrlsneCVNF 337 fruit fly
NP_650831 256 DNIFdp-efFMRLVEEHQITWIIQAPAHMAMMVNSPSft-tsDLSSLRYYLFGGSRAsvetQHRIRSRLSk----dCLHF 329 fruit fly
NP_650160 254 SSKPfaadyFVYLVSKYSITYALIPPEHCCSLLDCPTat-peKLGSLTKLNFGGGRM----TQATVERVKklapngVLNS 328 fruit fly
NP_650832 254 TDRPytpeyMIQLVEKYKVTLLTVVPQQVASLLKTPTln-kqRLASIRFVSVGGGSCyvanLLKLQEFLIt----gQISY 328 fruit fly
NP_610779 254 SNKAftpeyFVGLVKKYKINYAVLPPRHLSALITCPDak-pdALAPITHLNYGGGSIslatLQRSQELCKt----aMFNS 328 fruit fly
NP_650830 259 SSEMfstahFLDICEQHEVSWTIMANSHVAMLANCPKts-aqKLRSLKHLLFAGGHClvatLKKMQSFLHgs---gILRN 334 fruit fly
NP_611913 257 SRRTfsaetALYLVSKYKVTCLAMAPWQAYELFTSPLat-seQLESLKIAFVIGGWIslalLRRAQELLPk----tYVMF 331 fruit fly
Feature 1     #####                                                                           
2D1Q_A    341 GYGLTETTSa---IIITPEGddk-----pgASGKVVPlFKAKVIDldtkkslgpnrRGEVCVKGPMLMKGYVNNPEAtke 412 Japanese firefly
Q5BA81    355 AYGLTETGVv---ATLTSPHdvw-----hgSSGSLLPsFEIRLVKpdgtdaegldePGEVHFNSPSCFLGYVGDDESnkn 426 Aspergillus nidula...
3A9V_A    329 GYGMTEAGPv---LAMCLAFakepfdikpgACGTVVRnAEMKIVDpetgaslprnqPGEICIRGDQIMKGYLNDPEAtsr 405 Populus tomentosa
NP_647992 338 SYTLTELNSp---GCLNFNFdek-----pnSVGRPVRgIQIKIVNelgea-qgpnvVGEICFNNGQKWPGYYKNPEEtkk 408 fruit fly
NP_650831 330 AYGFTELGSm---AALNLHFdek-----pnSVGRLVAgLKLKVICekges-lgpdeVGELCLWNGQYWAGYYGNPEEthk 400 fruit fly
NP_650160 329 SYGMTEVGFivfnHGHLKLT----------AAGNPLPgIQVKIVDddgnq-lgvnqTGEIIVHNGFSWNGYFADPEAtka 397 fruit fly
NP_650832 329 GYALTECGGvaanMGVAKPS----------SVGRIVPgVRVKILDeagrs-lghgeTGEILVHNGKVWNGYYANPNEskr 397 fruit fly
NP_610779 329 GYGMTEVGA----ITINIGIsn------vsSAGRPVPgIKIRIVDedgks-lgynqVGEIYVHTGQAWNGYYGNPVEtrr 397 fruit fly
NP_650830 335 AYGLTEVGTl---VSYNYDTqsk-----ptSVGRLMAnIRVKIVDssgql-qgpkgLGEILCHNGQPWSGYVGNPLAtae 405 fruit fly
NP_611913 332 SYGTTETGV----VTINIDHsl------ecSVGRLAPgMRIKIQGedgqq-lgvnqTGEVLIDIGLKWEGYLSNPEDtat 400 fruit fly
Feature 1                #               #  # # # #    #                                      
2D1Q_A    413 lideeGWLHTGDIGYYde----eKHFFIVDRLKSLIKYKGYQVPPAELESVLLQHPsiFDAGVAGVPdpv-agELPGAVV 487 Japanese firefly
Q5BA81    427 tfdekGWLKSGDIGVFrkspnghAHLFILERIKDMIKVKGEQVLPRDIESVLLSHPavIDAAVIGVPdel-sgERAKAYI 505 Aspergillus nidula...
3A9V_A    406 tidkeGWLHTGDIGYIdd----dDELFIVDRLKELIKYKGFQVAPAELEALLIAHPeiSDAAVVGLKded-agEVPVAFV 480 Populus tomentosa
NP_647992 409 mqdseNWFHTGDLGYMde----dGYLFIIDRLKDMLKYQTIMYYPSEIESVIAEMPnvVEACVFGIWdpv-ygDKAAASV 483 fruit fly
NP_650831 401 mrdhhNWFHTGDLGYVdd----dGFIYIVERKKDMLKFQNIMYYPNEIESVISKMPdvVEVCVFGVWneingdEATAAVV 476 fruit fly
NP_650160 398 mqdeeGWFHTGDMGYFde----dDYLYMTDRKKEVLKWKGLQMWPAEVEAVIDELPevKRVCVIGVYdet-qgDVPGALV 472 fruit fly
NP_650832 398 mqdyqGWFHTGDMGYFdn----eNYLHIVERKEDLLRFHGAQYSPQEIEQVIAELPdvIEACVFGLWnev-dgDPAAAAV 472 fruit fly
NP_610779 398 mqdfeGWFHTGDLGYFde----qNFLYIVDRKKEILKYNGLHYWPTEIETVIAELSqvQDVCVVGIYder-egDAAGALV 472 fruit fly
NP_650830 406 mrdsaGWYHTGDVGYFde----dHYLHIVERKKDMLKYLGMMYYPHEVEEVIAQMPdvAEVCVFGIFret-egDAAAASV 480 fruit fly
NP_611913 401 t-lqdGWINLGDLGYFde----dNNLYLVDRKKDLLKYKSKHYWPNEIEQIIAELPevEHVCVVGVRdar-ygDAAGALI 474 fruit fly
Feature 1                                                          
2D1Q_A    488 VLesg------knmTEKEVMDYVASQVs-nAKRLRGGVRFVDEVPKGLTGKID 533 Japanese firefly
Q5BA81    506 VRsktvmedldeddLADEIDEFVQGKLh-eSHWLHDRIVFLEKLPKSESGKVL 557 Aspergillus nidulans FGSC A4
3A9V_A    481 VKsek------sqaTEDEIKQYISKQVi--FYKRIKRVFFIEAIPKAPSGKIL 525 Populus tomentosa
NP_647992 484 VKkqg------tqlEAQDVVEYVRKRIpakFKQLNGGALIVDHIIQSGNRKAN 530 fruit fly
NP_650831 477 KKrgs-------alTAQDIVDYVETHIdakYKQLNGGAIIVEDLVRSPNGKTN 522 fruit fly
NP_650160 473 VRedn------atlTAQQVIDHVAKRLpdiQKQLRAGVQFADEIPQNHNGKVV 519 fruit fly
NP_650832 473 VKipg------srlTEMDIVEYVAKRLvvdHKQLHCGVFFLPELPKTGSGKVL 519 fruit fly
NP_610779 473 VKskg------atiSAKEIVEHVAKRLpatQKQLRAGVQFTDKLPANVNGKTM 519 fruit fly
NP_650830 481 VLrsg------sklDPKHVEQYVRKNVsvqFKHLHGGVQFVPQLAKSANGKVN 527 fruit fly
NP_611913 475 IKkeg------aeiADQKVIDHVAQRVvvdYKQLNAGVIFVDKFPKNANGKVM 521 fruit fly

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