1MX3,1HKU,1HL3,2OME,3GA0


Conserved Protein Domain Family
CtBP_dh

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cd05299: CtBP_dh 
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C-terminal binding protein (CtBP), D-isomer-specific 2-hydroxyacid dehydrogenases related repressor
The transcriptional corepressor CtBP is a dehydrogenase with sequence and structural similarity to the d2-hydroxyacid dehydrogenase family. CtBP was initially identified as a protein that bound the PXDLS sequence at the adenovirus E1A C terminus, causing the loss of CR-1-mediated transactivation. CtBP binds NAD(H) within a deep cleft, undergoes a conformational change upon NAD binding, and has NAD-dependent dehydrogenase activity.
Statistics
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PSSM-Id: 240624
Aligned: 76 rows
Threshold Bit Score: 287.488
Created: 8-Nov-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 25 residues -Click on image for an interactive view with Cn3D
Feature 1:NAD binding site [chemical binding site]
Evidence:
  • Structure:1MX3: human Ctbp Dehydrogenase binds NAD; contacts to NAD at 4A
  • Structure:2OME: Human Ctbp2 Dehydrogenase binds NAD; contacts at 4A
  • Structure:1HL3: Rat CtBP BARS binds NAD; contacts at 4A
  • Comment:[AG]xGxxG(17-18x)[DN]: NAD-binding site motif characteristic of FDH and related dehydrogenases
  • Citation:PMID 8053888

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                 #       
1MX3_A         22 PLVALLDgrdcTVEMPILKdvATVAFCDAQsTQEIHEKvlnEAVGALMYHTiTLTREDLEKFKaLRIIVRIGSGFDNIDI 101  human
2OME_A          6 PLVALLDgrdcTVEMPILKdlATVAFCDAQsTQEIHEKvlnEAVGAMMYHTiTLTREDLEKFKaLRVIVRIGSGYDNVDI 85   human
3GA0_A         25 PLVALLDgrdcTVEMPILKdvATVAFCDAQsTQEIHEKvlnEAVGALMYHTiTLTREDLEKFKaLRIIVRIGSGFDNIDI 104  Norway rat
Q9YHU0         28 PLVALLDgrdcTVEMPILKdvATVAFCDAQsTQEIHEKvlnEAVGALMYHTiTLTREDLEKFKaLRIIVRIGSGFDNIDI 107  African clawe...
O46036         28 PLVALLDgrdcSIEMPILKdvATVAFCDAQsTSEIHEKvlnEAVGALMWHTiILTKEDLEKFKaLRIIVRIGSGTDNIDV 107  fruit fly
AAX20023      777 PLVALLDgrdcTVEMPILKdlATVAFCDAQsTQEIHEKvlnEAVGAMMYHTiTLTREDLEKFKaLRIIIRIGSGYDNIDI 856  zebrafish
NP_001012908   17 PLVALLDgrdcTVEMPILKdvATVAFCDAQsTQEIHEKvlnEAVGALMYHTiTLTREDLEKFKaLRIIVRIGSGFDNIDI 96   chicken
EDP32333      160 PLVALLDgrdcTVEMPILKdvATVAFCDAQsTHEIHEKvlnEAVAALMWHSiTLEREDLEKFKaLRVVVRIGTGTDNIDV 239  agent of lymp...
EEC11318       35 PLVALLDgrdcSVEMPILKdvATVAFCDAQsTQEIHEKvlnEAVGALMWHTiTLTKEDLEKFKaLRIIVRIGSGVDNIDT 114  black-legged ...
CBY16359       11 PLVALLDgrdcSIEMPILKdvATVAFCDAQsTSEIHEKvltEAVGALVWHNiRLGREDLEKFKaLKIIVRIGTGLDNIDV 90   Oikopleura di...
Feature 1                             #                                                    ## ### 
1MX3_A        102 KSAGDLGIAVCNVPAaSVEETADSTLCHILNLYRRAtwLHQALREGtrvqsveqi-revasgaaRIRGETLGIIGLGRVG 180  human
2OME_A         86 KAAGELGIAVCNIPSaAVEETADSTICHILNLYRRNtwLYQALREGtrvqsveqi-revasgaaRIRGETLGLIGFGRTG 164  human
3GA0_A        105 KSAGDLGIAVCNVPAaSVEETADSTLCHILNLYRRTtwLHQALREGtrvqsveqi-revasgaaRIRGETLGIIGLERVG 183  Norway rat
Q9YHU0        108 KSAGDLGIAVCNVPAaSVEETADSTMCHILNLYRRTtwLHQALREGtrvqsveqi-revasgaaRIRGETLGIIGLGRVG 186  African clawe...
O46036        108 KAAGELGIAVCNVPGyGVEEVADTTMCLILNLYRRTywLANMVREGkkftgpeqv-reaahgcaRIRGDTLGLVGLGRIG 186  fruit fly
AAX20023      857 KAAGEMGIAVCNIPSaAVEETADSTLCHILNLYRRNtwLYQAMREGtrvqsveqi-revasgaaRIRGETLGLIGFGRSG 935  zebrafish
NP_001012908   97 KSAGDLGIAVCNVPAaSVEETADSTMCHILNLYRRTtwLHQALREGtrvqsveqi-revasgaaRIRGETLGIIGLGRVG 175  chicken
EDP32333      240 KAATDLGIAVCNTPGdCVEEVADTTVSLILNMYRKTfwLAKAVSEGkkvsgveqv-relasgstRIRDDTLGIIGLGRVG 318  agent of lymp...
EEC11318      115 KAAGELGIAVCNVPGyGVEEVADTTLCLILNLYRRTywLANMVREGkkfqgpeqv-reaaqgcaRIRGDTLGIVGLGRVG 193  black-legged ...
CBY16359       91 QTATDMGISVISLGGaCTEEVADTTMCHILNLYRKVtyLHQAITQKgkkpqnaeqvreladgcmRIRGEKLGIIGLGAIG 170  Oikopleura di...
Feature 1                         #####                            ### #  #                    ###
1MX3_A        181 QAVALRAKAFGfNVLFYDPYLSDgveralGLQRVSTLQDLLfHSDCVTLHCGLNeHNHHLINDFTVKQMRQGAFLVNTAR 260  human
2OME_A        165 QAVAVRAKAFGfSVIFYDPYLQDgierslGVQRVYTLQDLLyQSDCVSLHCNLNeHNHHLINDFTIKQMRQGAFLVNAAR 244  human
3GA0_A        184 QAVALRAKAFGfNVLFYDPYLSDgieralGLQRVSTLQDLLfHSDCVTLHCGLNeHNHHLINDFTVKQMRQGAFLVNTAR 263  Norway rat
Q9YHU0        187 QAVALRAKTFGfNVFFYDPYLSDgieralGLQRVSTLQDLLfHSDCVTLHCGLNeHNHHLINDFTIKQMRQGAFLVNTAR 266  African clawe...
O46036        187 SAVALRAKAFGfNVIFYDPYLPDgidkslGLTRVYTLQDLLfQSDCVSLHCTLNeHNHHLINEFTIKQMRPGAFLVNTAR 266  fruit fly
AAX20023      936 QAVAVRAKAFGfNVIFYDPYLQDglerslGVQRVYTLQDLLyQSDCVSLHCNLNeHNHHLINDFTIKQMRQGAFLVNTAR 1015 zebrafish
NP_001012908  176 QAVALRAKAFGfNVIFYDPYLSDgmeralGLQRVSTLQDLLfHSDCVTLHCNLNeHNHHLINDFTIKQMRQGAFLVNTAR 255  chicken
EDP32333      319 TAVAMRAKAFGfKICFFDPHLPEgvdrslGIERCYNLDDILfKSDCITLHCPLTdETRHMINDMTIKQMRPGAFIVNTSR 398  agent of lymp...
EEC11318      194 TAVALRARVFGfNVIFYDPYLPDgiekslGLSRVYTLQDLLfQSDCVSLHCTLNeHNHHLINEYTMKQMRPGAFLVNTAR 273  black-legged ...
CBY16359      171 TAVALRAKVFGfKILYYDPLIPDgkgqalGLQRARSLQDLLyEADCISLHASLNeNSRNMFDVFAFQKMRKGAFFVNTAR 250  Oikopleura di...
Feature 1                                ##                        # ##                           
1MX3_A        261 GGLVDEKALAQALKEGRIRGAALDVHesEPFsf-sqGPLKDaPNLICTPHAAWYSEQASIEMREEAAREIRRAITGRIPD 339  human
2OME_A        245 GGLVDEKALAQALKEGRIRGAALDVHesEPFsf-aqGPLKDaPNLICTPHTAWYSEQASLEMREAAATEIRRAITGRIPE 323  human
3GA0_A        264 GGLVDEKALAQALKEGRIRGAALDVHesEPFsf-sqGPLKDaPNLICTPHAAWYSEQASIEMREEAAREIRRAITGRIPD 342  Norway rat
Q9YHU0        267 GGLVDEKALAQALKEGRIRGAALDVHesEPFsf-tqGPLKDaPNLICTPHAAWYSEQASIEMREEAAREIRRAITGRIPD 345  African clawe...
O46036        267 GGLVDDETLALALKQGRIRAAALDVHenEPYnv-fqGALKDaPNLICTPHAAFFSDASATELREMAATEIRRAIVGNIPD 345  fruit fly
AAX20023     1016 GGLVDEKALAQALKEGRIRGAALDVHesEPFsf-tqGPLKDaPNLICTPHTAWYSEQASLEMREAAATEIRRAITGRIPD 1094 zebrafish
NP_001012908  256 GGLVDEKALAQALKEGRIRGAALDVHesEPFsf-sqGPLKDaPNLICTPHAAWYSEQASIEMREEAAREIRRAITGRIPD 334  chicken
EDP32333      399 GGLIQESALGESLKSGHIKAAALDVHehEPFdplamGPLSAvPNIIHTPHCSWYSDASCKELRLSAAREVRRAIVGRCPH 478  agent of lymp...
EEC11318      274 GGLVDEAALAAALKDGRIRAAALDVHenEPYnt-laGPLKEaPNLICTPHAAWYSDASCTELREMAATEIRRAIVGRIPD 352  black-legged ...
CBY16359      251 GELVDEAALAAALKSGQVRAAAIDVLssELFdf-qkSPLRDsPNLYVTPHSAWYSDQSLKEVRENAATEMRLALHGLDNP 329  Oikopleura di...
Feature 1          
1MX3_A        340 S 340  human
2OME_A        324 S 324  human
3GA0_A        343 S 343  Norway rat
Q9YHU0        346 S 346  African clawed frog
O46036        346 V 346  fruit fly
AAX20023     1095 S 1095 zebrafish
NP_001012908  335 S 335  chicken
EDP32333      479 D 479  agent of lymphatic filariasis
EEC11318      353 S 353  black-legged tick
CBY16359      330 T 330  Oikopleura dioica

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