2G07,2VKQ,2G09,4NV0


Conserved Protein Domain Family
HAD_5NT

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cd07504: HAD_5NT 
Click on image for an interactive view with Cn3D
haloacid dehalogenase (HAD)-like 5'-nucleotidases similar to human cytosolic IIIA and IIIB
5'-nucleotidases dephosphorylate nucleoside 5prime-monophosphates. This family includes human 5'-nucleotidase, cytosolic IIIA (cN-IIIA, previously called cN-III; NT5C3A) the main pyrimidine 5'-nucleotidase in erythrocytes which dephosphorylates the pyrimidine nucleotides CMP, UMP, TMP, and the purine 7-methylguanosine monophosphate (m7GM), and possesses phosphotransferase activity. It also includes human 5'-nucleotidase, cytosolic IIIB (cN-IIIB; NT5C3B) which has a strong preference for m7GMP, dephosphorylates CMP and UMP and, with significantly lower efficiency, GMP and AMP, and can also act as a phosphotransferase. This family belongs to the haloacid dehalogenase-like (HAD) hydrolases, a large superfamily of diverse enzymes that catalyze carbon or phosphoryl group transfer reactions on a range of substrates, using an active site aspartate in nucleophilic catalysis. Members of this superfamily include 2-L-haloalkanoic acid dehalogenase, azetidine hydrolase, phosphonoacetaldehyde hydrolase, phosphoserine phosphatase, phosphomannomutase, P-type ATPases and many others. HAD hydrolases are found in all three kingdoms of life, and most genomes are predicted to contain multiple HAD-like proteins. Members possess a highly conserved alpha/beta core domain, and many also possess a small cap domain, the fold and function of which is variable. HAD hydrolases are sometimes referred to as belonging to the DDDD superfamily of phosphohydrolases.
Statistics
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PSSM-Id: 319807
Aligned: 16 rows
Threshold Bit Score: 388.98
Created: 20-Mar-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 21 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:based on cytosolic 5'-nucleotidase III and on other haloacid dehalogenase superfamily members with structure
  • Structure:2VKQ; human cytosolic 5'-nucleotidase III binds beryllium trifluoride and Mg2+ in the active site
  • Structure:2G09; mouse pyrimidine 5'-nucleotidase type 1 complex binds phosphate ion and Mg2+ in the active site
  • Structure:4NV0: Drosophila melanogaster cytosolic 5'-nucleotidase IIIB binds 7-methylguanosine, trifluoromagnesate and Mg2+ in the active site

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                #####               #                     #   #  #      
2G07_A        25 NPTRVEEIICGLIKGGaaKLQIITXFDXTLSRFSYNGKRcp-tCHNIIDNckl-vtDECRRKLLQLKEQYYAIEVDPvlt 102 house mouse
2VKQ_A        20 NPTRVEEIICGLIKGGaaKLQIITDFDMTLSRFSYKGKRcp-tCHNIIDNckl-vtDECRKKLLQLKEKYYAIEVDPvlt 97  human
NP_001088032  14 DPERVEELICRLIKGGasKLQVITDFDMTLSKFSHNGKRcp-tCHNIIDNskl-itDECRKKLFQLKEKYYAIEIDPdlt 91  African clawed ...
Q969T7        17 QPGRVQEIVGALRKGGgdRLQVISDFDMTLSRFAYNGKRcp-sSYNILDNski-isEECRKELTALLHHYYPIEIDPhrt 94  human
Q7ZWS2        15 DPEGLQDKITRIQRGGqeKLQIISDFDMTLSRFSRNGERcp-tCYNIIDNsni-isDEGRKKLKCLFDIYYPLEIDPkks 92  African clawed ...
NP_001255892  20 DIKSLTDKLQNIVSGGadQLMVISDFDFTLSRFADKSGNrcssCYCVFDSavgtnnPEWCRKFVGLYHKYGPIEHDHsls 99  Caenorhabditis ...
7291759       31 DPAEVERIINEFVIGGpeRMQIVSDFDYTITKQRTEDGGavpsSFGIFNAcqs-lpENFKAETDKLYHKYRPIEIDPhmp 109 fruit fly
Q9W197        31 DPAEVERIINEFVIGGpeRMQIVSDFDYTITKQRTEDGGavpsSFGIFNAcqs-lpENFKAETDKLYHKYRPIEIDPhmp 109 fruit fly
22096397      95 DPTAVAAKLRKMVVGGagKTVVISDFDYTLSRFANEQGErlstTHGVFDDnvmrlkPELGQKFVDLKNKYYPIEFSPnlt 174 Caenorhabditis ...
NP_181402     14 HPRALTDKMTLIRDAGpsKFQVIADFDATLTRYRVNGLRg--rQTSHGLLqq--gdAYYDAKRQALYDHYHPLEISPvip 89  thale cress
Feature 1                  ##  #                                              ###                
2G07_A       103 veEKFPYXVEWYTKSHGLLieqgipkAKLKEIVADSDvxLKEGYENFFGKLQqhgIPVFIFSAGIGDVLEEVIRQagvy- 181 house mouse
2VKQ_A        98 veEKYPYMVEWYTKSHGLLvqqalpkAKLKEIVAESDvmLKEGYENFFDKLQqhsIPVFIFSAGIGDVLEEVIRQagvy- 176 human
NP_001088032  92 mkEKYPYMVEWYMKSHTLLidqrlqkDKLAEAVRESDvkLKEGYEMFFDILHkqsIPVFIFSAGLGDILEEVIRQagvy- 170 African clawed ...
Q969T7        95 vkEKLPHMVEWWTKAHNLLcqqkiqkFQIAQVVRESNamLREGYKTFFNTLYhnnIPLFIFSAGIGDILEEIIRQmkvf- 173 human
Q7ZWS2        93 ieEKYPLMVEWWSKAHDLFyeqriqkDRLAQVVKESQatLRDGYDLFFNSLYqreIPLFIFSAGIGDVLEEIIRQagvf- 171 African clawed ...
NP_001255892 100 ieEKVPFMEAWWQQSHGLIiqggfkkQAIDDYVAHCNiqLRDNADIMMKKMTnhaVPFIIFSAGIGTIIEMYLRHkfgrv 179 Caenorhabditis ...
7291759      110 iaEKVQYMIEWWTKSGELTsgfpfdqSEIDQIASKYThaLRDRTHEFFADLQrlgIPTLVFSAGLGNSVVSVLRQanvl- 188 fruit fly
Q9W197       110 iaEKVQYMIEWWTKSGELTsgfpfdqSEIDQIASKYThaLRDRTHEFFADLQrlgIPTLVFSAGLGNSVVSVLRQanvl- 188 fruit fly
22096397     175 meEKIPHMEKWWGTSHSLIvnekfskNTIEDFVRQSRivFKDGAEDFIEALDahnIPLVIFSAGIGNIIEYFLQQklgai 254 Caenorhabditis ...
NP_181402     90 idEKTKLMEEWWGKTHELLieggltyDAIKKSVANSSiaFREGVSELFEFLEkkeIPVLIFSAGLADVIEEVLRQnldrt 169 thale cress
Feature 1                                    #  #                                      ##  ##    
2G07_A       182 hsnVKVVSNFXDFDEnGVLKGFKGELIHVFNKHDGAL---------KNTDYFSql------kdNSNIILLGDSQGDLRXA 246 house mouse
2VKQ_A       177 hpnVKVVSNFMDFDEtGVLKGFKGELIHVFNKHDGAL---------RNTEYFNql------kdNSNIILLGDSQGDLRMA 241 human
NP_001088032 171 hpdVKVISNFMEFDEnGIITGFKGELIHVYNKHDGAL---------KNTEYFGhl------kdNSNIILLGDSLGDLAMA 235 African clawed ...
Q969T7       174 hpnIHIVSNYMDFNEdGFLQGFKGQLIHTYNKNSSAC---------ENSGYFQql------egKTNVILLGDSIGDLTMA 238 human
Q7ZWS2       172 hpnTKVVSNYMDFDDnGILTGFKGDLIHTYNKNSSVL---------KDTEYFKei------shRTNILLLGDTLGDLTMA 236 African clawed ...
NP_001255892 180 esnTHVVSNMMGFDDdGYVNSFSDPLIHVFCKNSSVMp--------ADRTFSEqi------egRKNVILLGDSVGDAFMD 245 Caenorhabditis ...
7291759      189 hpnVKVVSNFLQFRD-GLLDGFQQPMIHTFNKNETVL---------NETSEYYdlv-----htRDHIIVMGDSIGDADMA 253 fruit fly
Q9W197       189 hpnVKVVSNFLQFRD-GLLDGFQQPMIHTFNKNETVL---------NETSEYYdlv-----htRDHIIVMGDSIGDADMA 253 fruit fly
22096397     255 prnTHFISNMILFDEdDNACAFSEPLIHTFCKNSSVIq--------KETSFFHdi------agRVNVILLGDSMGDIHMD 320 Caenorhabditis ...
NP_181402    170 fknVKIVSNRMVFNDdGQLVSFKGKLIHVLNKNEHALdmaaplhdrLGVDIGEedeenvnmkeRRNVLLMGDHLGDLRMS 249 thale cress
Feature 1                                                                 
2G07_A       247 DGvanveHILKIGYLNDrvd--elLEKYXDSYDIVLvke----eslEVVNSILQKTL 297 house mouse
2VKQ_A       242 DGvanveHILKIGYLNDrvd--elLEKYMDSYDIVLvqd----eslEVANSILQKIL 292 human
NP_001088032 236 DGvpnveNVLKIGFLNDkve--elIEKYMDSYDIVLvkd----dtlDVANSIIQKIL 286 African clawed frog
Q969T7       239 DGvpgvqNILKIGFLNDkve--erRERYMDSYDIVLekd----etlDVVNGLLQHIL 289 human
Q7ZWS2       237 DGvstveNIIKIGFLNDkve--elTEQFLQSYDIVLlrd----etlDVVNGILQFVT 287 African clawed frog
NP_001255892 246 VGveeeqVSLKIGFVNHdad--klVDKYLNYFDIVLvdd----qsmDIPNQILEAIY 296 Caenorhabditis elegans
7291759      254 SGvpassHIMKIGFLFDhve--anMKKYMDTFDIVLvdd----qtmDVPRTLLSLIE 304 fruit fly
Q9W197       254 SGvpassHIMKIGFLFDhve--anMKKYMDTFDIVLvdd----qtmDVPRTLLSLIE 304 fruit fly
22096397     321 VGverdgPTLKVGYYNGslddtaaLQHYEEVYDIVLihd----ptlNVAQKIVDIIN 373 Caenorhabditis elegans
NP_181402    250 DGld-yeTRISIGFLNDnie--ksLESYRESFDLVYlndapmwgalELVSRLFSTEA 303 thale cress

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