1FLG


Conserved Protein Domain Family
PQQ_ADH_I

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cd10277: PQQ_ADH_I 
Click on image for an interactive view with Cn3D
Ethanol dehydrogenase, a bacterial quinoprotein (PQQ-dependent type I alcohol dehydrogenase)
This bacterial family of homodimeric ethanol dehydrogenases utilize pyrroloquinoline quinone (PQQ) as a cofactor. It represents proteins whose expression may be induced by ethanol, and which are similar to quinoprotein methanol dehydrogenases, but have higher specificities for ethanol and other primary and secondary alcohols. Dehydrogenases with PQQ cofactors, such as ethanol, methanol, and membrane-bound glucose dehydrogenases, form an 8-bladed beta-propeller.
Statistics
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PSSM-Id: 199835
Aligned: 20 rows
Threshold Bit Score: 742.192
Created: 18-Apr-2011
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:binding site for calcium and cofactor PQQ (pyrrolo-quinoline quinone)
  • Structure:1FLG: Ethanol dehydrogenase from Pseudomonas aerugionosa binds Ca2+ and PQQ

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                #                    
1FLG_A      8 IANDDKTTGDVLQYGMGTHAQRWSPLKQVNADNVFKLTPAWSYSFGde------kQRGQESQAIVSdGVIYVTASY---S 78  Pseudomonas aerugi...
ABI91706   42 RLTDAQRDPGWLTYYRTYNGQSHSPLKQIDPSNVKQLTQAWSYKFPaq------lKQGFEATPIVNgRYLFVTTPK---D 112 Burkholderia ambif...
ABQ30945   39 RLDNAAASKGWLMFNRSYAGDDYAPFAQINTKNVASLKPAWSSAKId-------iPNGFEGTPIVNgDYMFATTPL---D 108 Acidiphilium crypt...
AEA22996   36 ILEARSEPQNWLTYYGAYNGQRFSPLDQINTENVKRIGPAWVFQAGssgmiagasTYAFEAAPLVVdGVMFVSGWD---G 112 Pseudonocardia dio...
ACB97191   36 RLGAAAKDAGWLMYRRDYTGSGYAPFDTINTSNVANLKQVWDYKTDf--------DMGHESPAIVNgDYLFITTPK---N 104 Beijerinckia indic...
ABQ36007   41 ARLENPEPGNWMLYRRTYDGQGYSPLDQINTTNVKNMVPVWTFATGv--------IEGHEAPPLVNnGVMFITTPM---G 109 Bradyrhizobium sp....
ABM94553   58 LLNADKDANNWLHYGKDYQNTRFSALTQITTDNVKKLVPKWNLSFGv--------NEAQDSQTTVVnGRIYVTASQ---N 126 Methylibium petrol...
ABM93325   32 ARLTNPEAKNWLMYRGNYAGWGYSPLEKINDKNVSKLTLAWSYVTGm--------SEGHQSPPIVNnGYMYVTTPN---N 100 Methylibium petrol...
ABM60638   33 ARLANPEPENWLLTKGNYAGWSYTPLEQITPANVKRLRPVWSAATGv--------NSGHEAPAIVNgEYMFVATPH---N 101 Verminephrobacter ...
ABG63987   11 EMLTTPADGDWPNYRRTYDGWGYSPLNQITTDNVADMEPVWMISTGa--------AEGHESPPMVInGIMFVTQATttsN 82  Mesorhizobium sp. ...
Feature 1                                 ##    #                                            #
1FLG_A     79 RLFALDAKTGKRLWTYNHRLPDDIr--PCCDVVNRGAAIYGDKVFFGTLDASVVALNKNTGKVVWKKKFADHGa-GYTMT 155 Pseudomonas aerugi...
ABI91706  113 NVYAFDAATGAQLWKYEPKLGAESfktACCDVVNRGVALYGKNVYVAMLSGEVAALDAQTGALVWKKQMFEPGl-GYSFS 191 Burkholderia ambif...
ABQ30945  109 HLIAFNAKTGQKLWEYDYDLPKTAlktVCCDVNNRGVAVYGNEVFMGTLDNHVLALDATTGKVLWNVALEKPGi-GYSIS 187 Acidiphilium crypt...
AEA22996  113 WVWALNAATGNEIWRYKHATPYDVs--LCCGNVNRGVAVAKGKVFSVTPNAHLIALDATTGKKLWDRTYGDVRa-GESAT 189 Pseudonocardia dio...
ACB97191  105 ELHAFQASTGKPLWVYKHDLTGVGlktICCDVVNRGVALYGDNVYMATLDNRVVALDAKTGKPVWNTQLEKPDv-GYAMT 183 Beijerinckia indic...
ABQ36007  110 QVIALNAKTGDQYWRYKRELPEDL---FQLHPTNRGVGLWQDKLYLATTDDHLVALDAKTGKVLWDKKVQDYKk-GQYLT 185 Bradyrhizobium sp....
ABM94553  127 KVFSLDGVTGKMLWKYERSLPGDLgpkLCCDAVNRGVAVYKDMVFMATLDTHVVALNNTTGQVVWERKMGDYQt-GEIYT 205 Methylibium petrol...
ABM93325  101 QVIAFEAKSGKELWRYKKQIPEEL---QQLHPTNRGVALYNDKLYLATTDAMLVALDPVTGKELWKSSVGDWKa-GYYST 176 Methylibium petrol...
ABM60638  102 QVIAYHAKTGKVLWKFVREVPEGL---GALHRTNRGVSLYGDKVYVGSIDCMISALDAKTGKLLWDAQVCDWEkdSAYIT 178 Verminephrobacter ...
ABG63987   83 QVMAIKADTGEILWRYQRALPEDM---HSSHPTNRGVGFWGDKIFFTSRDAVLVALDAKSGAEVWTAPIDDYKn-GYYAN 158 Mesorhizobium sp. ...
Feature 1                          ##                                                         
1FLG_A    156 GAPTIVKdgktgkVLLIHG-SSGDeFGVVGRLFARDPDTGEEIWMRPFVEGHMGRlngkdstvtgdvkapswpddrnspT 234 Pseudomonas aerugi...
ABI91706  192 LAPLAID------GAIVVGsSGGE-YGARGFIAALSPNDGALLWKRYTIPGQGEKha---------------------dT 243 Burkholderia ambif...
ABQ30945  188 EAPMIVD------GKVIIGsGGGE-YGARGFIVGLDAQTGKLLWKTYTIPSPSEPgg---------------------kT 239 Acidiphilium crypt...
AEA22996  190 CAPLIVK------NLVIVGsSGGE-FGVRGHLDAFDLDSGEHAWRCYMVPKPGEPgsd-------------------twP 243 Pseudonocardia dio...
ACB97191  184 VAPLALK------GKVVVGvSGGE-YGARGYIAALDASTGKEVWRRHTIPAPNEPgg---------------------dT 235 Beijerinckia indic...
ABQ36007  186 LMPLIVD------GKVIVGgSGGE-FGVRGYVVAYDAEDGKELWRTFTIPGEGEPgh---------------------dT 237 Bradyrhizobium sp....
ABM94553  206 SMPLVAK------GMVIVGsASGDvGANVGRITALDPATGNTIWEFKTRPTSADHpq--------------------akT 259 Methylibium petrol...
ABM93325  177 LAPLVAN------GKVVTGvSGGE-YGVRGYVIALDAETGKEAWRTYTIPAPGEPgg---------------------dT 228 Methylibium petrol...
ABM60638  179 SAPLVVK------GKVLIGpSGGE-FGVRGFLKAFDAETGKLAWTRYAVPGPGEAgfet----------------wpqdG 235 Verminephrobacter ...
ABG63987  159 LAPLVID------GKVIVGtSGGE-RGIRGFVAAFNADDGKEVWRTYTIPAPGEPgs--------------------etW 211 Mesorhizobium sp. ...
Feature 1                  #                 #                                                
1FLG_A    235 GKVESWSHGGGAPWQSASFDAETNTIIVGAGNPGPWNTwartakggnpHDYDSLYTSGQVGVDPSSGEVKWFYQHTPNDA 314 Pseudomonas aerugi...
ABI91706  244 WPDGMQAHGGAPAWLTGTYDAATRTLYWGVGNPGPWLAd--------lRPGDNLYSDSLLALDPKNGDLKWHYQYTKHDT 315 Burkholderia ambif...
ABQ30945  240 WPAGAYKHGGGSPWMTGTYDPKTKQLFWGVGNPGPWLAs--------mRPGNNLYSDSVIAIDPNTGAIKWHYQYTRNDT 311 Acidiphilium crypt...
AEA22996  244 SEGQAWARGGGNCWLTGTFDPETNLLYWGTGNPAPDFDge-------vREGDNLYTDSIVAIDADAGEIRWHYQCTPHDV 316 Pseudonocardia dio...
ACB97191  236 WPEGAYKTGGGPAWLTGSYDADTDTLFWGVGNPGPWLAt--------lRPGDNLYTDSVLALDPATGNIKWHYQYTPNDT 307 Beijerinckia indic...
ABQ36007  238 WSGDDWKSGGGSAWMTGTYDTDTKTVYWGVGNAAPWPGg--------lHPGDNLYTSSVIALDPATGKIKTHFQYHQNDS 309 Bradyrhizobium sp....
ABM94553  260 WANDSWKTGGGAAWLTGTYDAKTNTVLWGVGNPSPAFDps-------vRKGDNLYTNSTVALDADTGKIKYHFQYTPNDA 332 Methylibium petrol...
ABM93325  229 WPGETYKKGGGSVWITGTYDPETKLSYWGTGNAAPWMGd--------tRAGDNLYSTSVIALDIETGKLKGYHQYHWNDT 300 Methylibium petrol...
ABM60638  236 QWKDAWKNGGGTMWMPGNYDAKNDVIYWGVGNGAPWLGd--------qRPGDNLYLASVLAISPDTGNIVSHFQYHWNDS 307 Verminephrobacter ...
ABG63987  212 PDNEAWKTGAGSTWITGHYDPENKLIYWGVGNGGPWMGd--------qRPGDNLYVTSVIALDPESGEIKGHFQYHPNDS 283 Mesorhizobium sp. ...
Feature 1      #                           #                                                  
1FLG_A    315 WDFSGNNELVLFDykakdGKIVKATAHADRNGFFYVVDR---SNGKLQNAFPFVDNITWashiDLKTGRPVeregqrppl 391 Pseudomonas aerugi...
ABI91706  316 WDYDGVNTPVLATik-ykGKEHDAIIHADRNGYFHAIDR---DTGKLIYAEPFVKATSVt--gYTEDGAPIqdpak---- 385 Burkholderia ambif...
ABQ30945  312 WDYDGVNTAQLADit-ykGKPYHALVEANRNGYFYAINR---ETGKLIYAVPFVHDTSIi---GIKDGQAVnnmkl---- 380 Acidiphilium crypt...
AEA22996  317 WDYDSISECILFE-----HEGRKLLGHFDKNGYFFVVDR---TNGELVRITPFVDRIDWg--nITRDGKVTpsry----- 381 Pseudonocardia dio...
ACB97191  308 WDYDGTNEQVLIDit-heGKPYKALVSASRNGWLYAIDR---TNGKLIYGEKFATATSVt---GFKDGKPVtdpdk---- 376 Beijerinckia indic...
ABQ36007  310 WDWDEVDAPMLVDlq-rdGRSFKSLIHPGRDAIFWVLERt-pERINYVNGWTFVHTDVWkg-iEPETGRPIvdpah---- 382 Bradyrhizobium sp....
ABM94553  333 WDYDGNNEMILVDd----DKGRKVWLHGDRNGHLYSIDR---TNGKCNWVVPIARVNWVt--gFGENCRPIvnpdk---- 399 Methylibium petrol...
ABM93325  301 WDWDEVSAPLLVDvd-rnGKKVKSLVHAGRNGYLWQLERt-sGPIKFVDAWPYVKQNVFts-iDPKTGRPTydesr---- 373 Methylibium petrol...
ABM60638  308 WDWAGMNAPTLVEfn-rsGKKVSGLISAQRNGRLYWLERdgkGKITYNKSEAYVTNTVFas-iDPKTGRPTyneea---- 381 Verminephrobacter ...
ABG63987  284 FDWDEVAAPILVDlpnkdGETVPGLVHAARNGVLWELERkpdGEIGFIKGTPYVKENWIks-mDPETGRVEyaegt---- 358 Mesorhizobium sp. ...
Feature 1                                                                                     
1FLG_A    392 pepgqkhGKAVEVSPPFLGGKNWNPMAYSQDTGLFYVPANH--WKEDYWTEEVSYTKGs------------aYLGMGFri 457 Pseudomonas aerugi...
ABI91706  386 ---ypkvGTTIDTCPSFLGGKNWWSVSYDPQRHLAIVPSLH--ACMSLSGKSVNYMEGl------------pYLGEGFei 448 Burkholderia ambif...
ABQ30945  381 ---rpalDKQIFTCPSFLGGKNWWPGAVDPATNMAYVPTLH--ACMTMKGVPVSYAAGl------------pYLGETFem 443 Acidiphilium crypt...
AEA22996  382 ---pekeGEPVHFFPGPAGAKEWTHAAYNPETELFYVPVQD--VGATATRRRREFKESi------------pYWGAGVqv 444 Pseudonocardia dio...
ACB97191  377 ---rpdiNKEIFTCPSFLGGKNWWPISVDVKTQIAYVPTMH--TCMTMKGNAVSYRAGl------------pFLGESFkv 439 Beijerinckia indic...
ABQ36007  383 ---kptlGKRVDFCPSLWGGKDWPSAAYSQKTRFVYVPANEn-FCGGFTGEKLPLVPGq------------lWLGTKAed 446 Bradyrhizobium sp....
ABM94553  400 --vpgydRVATDIAPILDGGKEWHPAAYSLRTKLIYVPFID--SSMDVQAKKSEWKKGe------------wFLSSKVlk 463 Methylibium petrol...
ABM93325  374 ---rpatGKTVNFCPSLWGGKDWVPEAYSPKTKMFYVPANNn-LCSELTGEAVTYKKGd------------lYIGVALdn 437 Methylibium petrol...
ABM60638  382 ---kpatGKTRTYCPSLWGGKDWPYEAYNPKTGMLYVPYNDn-HCLTLTGMVQSIVPG--------------QWSAGVdi 443 Verminephrobacter ...
ABG63987  359 ---kpgtGILGDFCPSFWGGKDWPPIAYDPETNYVFIPANEnlCTKMQGGTVVEYRPGasftrgtqgdrsdlYLAEGA-- 433 Mesorhizobium sp. ...
Feature 1                                            #                                        
1FLG_A    458 kr-------mydDHVGSLRAMDPvSGKVVWEHKEh-LPLWAGVLATaGNLVFTGTG-DGYFKAFDAKSGKELWKFQTGSG 528 Pseudomonas aerugi...
ABI91706  449 kp------epgsEGYGELQAIDVnTGKKVWSHWSk-LPWNGGVATTaSGLAFSGSL-DGHLYAFDVSTGKVLWESPKLAS 520 Burkholderia ambif...
ABQ30945  444 kpd-----paspHHMGSIQAIDLsTGKQVWQHRTk-EPWDGGAMATsGGLVFSGTA-DGHLYAFDAKTGKILWKSPYLGS 516 Acidiphilium crypt...
AEA22996  445 d---------ieDMTGYVSAYDA-NGEEKWRWRAk-LPMCASVLTTgGGLVFAGEP-NGMFNALDARTGEHLWQFQCGSG 512 Pseudonocardia dio...
ACB97191  440 vrd-----pafpNHWGAVQAIDLnTGKQVWDFPSe-LPWNDGTLTTaGGVVFSGSA-DGYLHAFDAKTGKVLWKSPQATS 512 Beijerinckia indic...
ABQ36007  447 iglt---vrpgaDHFGELQAWDPaSGKKVWSHNFpkSQLFGSVTATaGDLVLVGGTnDRMFRAFHAKTGELLWEQKTNSG 523 Bradyrhizobium sp....
ABM94553  464 ----------anPYTGGVKAFNAtTGEQVWTRPQs-TPATSGLLATaGKLVFSGDA-EGYFSALKDDTGEELWRFNVGSG 531 Methylibium petrol...
ABM93325  438 iltnvrmteaskTHVGEVQAWNLgDKKKAWTHTYp-EMNWGPLLATgGNLVFGGGTnDRLFRAFNASTGKLLWEFPASSG 516 Methylibium petrol...
ABM60638  444 nkldl-svkpgfDHIGGIQAWSVdGGKEVWRQKYphSWNWGSILTTgSGVLFAGGTnDRMFRAYDAKSGKLLWEFPTPSG 522 Verminephrobacter ...
ABG63987  434 ------------DHIGEVQAWDLsTGEKVWTHEFpnSANWGPLMTTaGGLVFGGGTnDNMFRAFDAKSGDIVWEMKLNSS 501 Mesorhizobium sp. ...
Feature 1                                                                
1FLG_A    529 I-VSPPITWEQDGEQYLGVTVGYGGAVPLWGGDMAdlt-------rpVAQGGSFWVFKL 579 Pseudomonas aeruginosa
ABI91706  521 GiIAQPSVFEVDGKEYVAVLAGYGGANPIWGGPMAkaa-------ddVPRGGTLYVFAL 572 Burkholderia ambifaria AMMD
ABQ30945  517 GiIANPMSYEIDGKQYVAVWSGWGGVWPLWSGKLGasl-------akAPRGGQLHVFAL 568 Acidiphilium cryptum JF-5
AEA22996  513 H-HSSPVSYSVDGRQFVAVPVGWGGWAEGFLPGMLg-----------AGHGSALMVFAL 559 Pseudonocardia dioxanivorans CB1190
ACB97191  513 GfLGVPSTWKVGDKQYIGIYAGWGGGTPIWGGDMAkdpr-----vlnIPLGGHFYVFSL 566 Beijerinckia indica subsp. indica ATCC ...
ABQ36007  524 I-MGMPVAYEVDGTQYIAVQSGWGVDAQRIQDALAkgntg---lednVPQGGVVWVFAV 578 Bradyrhizobium sp. BTAi1
ABM94553  532 I-HGNPTTFTADGKQYVAIVYGPGGGSLWPLVYDElf--------krNNRGGGMMVFGL 581 Methylibium petroleiphilum PM1
ABM93325  517 V-TGVPSSFEVDGEQYIAVQSGWGVDAERMQGAFNavlk----nktvVPQGGTVMVFKL 570 Methylibium petroleiphilum PM1
ABM60638  523 I-ISPPSSFSIGGKQYIAVVSGWGVDAQWIQGKMEglag----wekaVPEGGSVWVFAL 576 Verminephrobacter eiseniae EF01-2
ABG63987  502 V-IGVPSSFEVDGKQYVSVQAGWGIDAASMQSRLAtsypdkfkpgptVPTGGVIWVFAL 559 Mesorhizobium sp. BNC1

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