3EQ6,2WD9,3B7W,3C5E


Conserved Protein Domain Family
MACS_euk

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cd05928: MACS_euk 
Click on image for an interactive view with Cn3D
Eukaryotic Medium-chain acyl-CoA synthetase (MACS or ACSM)
MACS catalyzes the two-step activation of medium chain fatty acids (containing 4-12 carbons). The carboxylate substrate first reacts with ATP to form an acyl-adenylate intermediate, which then reacts with CoA to produce an acyl-CoA ester. The acyl-CoA is a key intermediate in many important biosynthetic and catabolic processes. MACS enzymes are localized to mitochondria. Two murine MACS family proteins are found in liver and kidney. In rodents, a MACS member is detected particularly in the olfactory epithelium and is called O-MACS. O-MACS demonstrates substrate preference for the fatty acid lengths of C6-C12.
Statistics
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PSSM-Id: 341251
Aligned: 11 rows
Threshold Bit Score: 1043.97
Created: 20-Sep-2011
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 35 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:3EQ6; Human medium chain acyl-CoA synthetase binds AMP and product anolog, contacts at 4A.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                        
3EQ6_A        33 VPAKFNFASDVLDHWADMEKAGKRPPSPALWWVNGKGKELMWNFRELSENSQQAANVLSgACGLQRGDRVAVVLPRVPEW 112 human
2WD9_A        33 VPAKFNFASDVLDHWADMEKAGKRPPSPALWWVNGKGKELMWNFRELSENSQQAANVLSgACGLQRGDRVAVVLPRVPEW 112 human
3B7W_A        33 VPAKFNFASDVLDHWADMEKAGKRPPSPALWWVNGKGKELMWNFRELSENSQQAANVLSgACGLQRGDRVAVVLPRVPEW 112 human
3C5E_A        33 VPAKFNFASDVLDHWADMEKAGKRPPSPALWWVNGKGKELMWNFRELSENSQQAANVLSgACGLQRGDRVAVVLPRVPEW 112 human
Q7TN78        48 LPKNFNFAADVLDQWSLKEKSGERPANPALWWVNGKGDEVKWSFQELGSLSRKAANVLTkPCGLQRGDRVAVILPRIPEW 127 Norway rat
Q08AH1        45 VPEEFNFASYVLDYWAQKEKEGKRGPNPAFWWVNGQGDEVKWSFREMGDLTRRVANVFTqTCGLQQGDHLALMLPRVPEW 124 human
NP_001086370  49 VPEYFNFANDILDEWARNRKEGGKSSNPALWWIDGSGEEIKWSFEELGFLSRKAANIISdACNLKPGDRVIVILPRVPEW 128 African clawed ...
Q53FZ2        54 IPEYFNFAKDVLDQWTDKEKAGKKPSNPAFWWINRNGEEMRWSFEELGSLSRKFANILSeACSLQRGDRVILILPRVPEW 133 human
NP_001104706  57 VPQRFNFAKDVLEKWETKEKSGQRSSLPALWWVNDHGTEVRWSFEELGFHSRKLANVLRqVCSLERGDRVFLILPRVPEW 136 zebrafish
P0C7M7        48 LPKNFNFAADVLDQWSQKEKTGERPANPALWWVNGKGDEVKWSFRELGSLSRKAANVLTkPCGLQRGDRLAVILPRIPEW 127 human
Q6NUN0        49 VPEYFNFAHDVLDVWSRLEEAGHRPPNPAFWWVNGTGAEIKWSFEELGKQSRKAANVLGgACGLQPGDRMMLVLPRLPEW 128 human
Feature 1                           #                                                            
3EQ6_A       113 WLVILGCIRAGLIFMPGTIQMKSTDILYRLQMSKAKAIVAGDEVIQEVDTVASECPSLRIKLLVSeksCDGWLNFKKLLN 192 human
2WD9_A       113 WLVILGCIRAGLIFMPGTIQMKSTDILYRLQMSKAKAIVAGDEVIQEVDTVASECPSLRIKLLVSeksCDGWLNFKKLLN 192 human
3B7W_A       113 WLVILGCIRAGLIFMPGTIQMKSTDILYRLQMSKAKAIVAGDEVIQEVDTVASECPSLRIKLLVSeksCDGWLNFKKLLN 192 human
3C5E_A       113 WLVILGCIRAGLIFMPGTIQMKSTDILYRLQMSKAKAIVAGDEVIQEVDTVASECPSLRIKLLVSeksCDGWLNFKKLLN 192 human
Q7TN78       128 WLINVACMRTGLVFMPGTIQLTRKDILYRLQASKAKCIVASEEVAPAVDSIASECPNLKTKLLVSphrWDGWLSFQELLQ 207 Norway rat
Q08AH1       125 WLVAVGCMRTGIIFIPATILLKAKDILYRLQLSKAKGIVTIDALASEVDSIASQCPSLKTKLLVSdhsREGWLDFRSLVK 204 human
NP_001086370 129 WLLNVACIRAGVVLIPGTTQLTAKDILHRLQTSMAKCIITSDSLAAAVDSVAAQSPCLETKMLISaerRDGWLDFNGLFR 208 African clawed ...
Q53FZ2       134 WLANVACLRTGTVLIPGTTQLTQKDILYRLQSSKANCIITNDVLAPAVDAVASKCENLHSKLIVSensREGWGNLKELMK 213 human
NP_001104706 137 WLVNVACIRTGTVLIPGTSQLTARDMRHRLITSGAKCVITDETLAPLLDTVALDCPSISTKLLLShkpMDGWGNLTELMG 216 zebrafish
P0C7M7       128 WLVNVACIRTGIIFMPGTIQLTAKDILYRLRASKAKCIVASEEVAPAVESIVLECPDLKTKLLVSpqsWNGWLSFQELFQ 207 human
Q6NUN0       129 WLVSVACMRTGTVMIPGVTQLTEKDLKYRLQASRAKSIITSDSLAPRVDAISAECPSLQTKLLVSdssRPGWLNFRELLR 208 human
Feature 1                                                                          ###           
3EQ6_A       193 EASTTHHCVETgsQEASAIYFTSGTSGLPKMAEHSYSSLGLKAKMDAG-WTGLQASDIMWTISDTGWILnILCSLMEPWA 271 human
2WD9_A       193 EASTTHHCVETgsQEASAIYFTSGTSGLPKMAEHSYSSLGLKAKMDAG-WTGLQASDIMWTISDTGWILnILCSLMEPWA 271 human
3B7W_A       193 EASTTHHCVETgsQEASAIYFTSGTSGLPKMAEHSYSSLGLKAKMDAG-WTGLQASDIMWTISDTGWILnILCSLMEPWA 271 human
3C5E_A       193 EASTTHHCVETgsQEASAIYFTSGTSGLPKMAEHSYSSLGLKAKMDAG-WTGLQASDIMWTISDTGWILnILCSLMEPWA 271 human
Q7TN78       208 SASEEHNCVQTgsQEPMAIYFTSGTTGSPKMAQHSQSSLGIGYALCGRyWLDLTSSDIMWNMSDTGWIKaAIGSVFSTWL 287 Norway rat
Q08AH1       205 SASPEHTCVKSktLDPMVIFFTSGTTGFPKMAKHSHGLALQPSFPGSRkLRSLKTSDVSWCLSDSGWIVaTIWTLVEPWT 284 human
NP_001086370 209 AADDKHICVKTksSDPMTIFFTSGTTGSPKMTEHSHCSYGHGLKVNGKyWMDLTPSDIVWNMSDTGWAKsAWSSVFAPWI 288 African clawed ...
Q53FZ2       214 HASDSHTCVKTkhNEIMAIFFTSGTSGYPKMTAHTHSSFGLGLSVNGRfWLDLTPSDVMWNTSDTGWAKsAWSSVFSPWI 293 human
NP_001104706 217 RVSDDHVCVDTssEEPMTIFFTSGTTGSPKMTQHSHCSYGLGLTVNGRyWLDLTEQDVFWNTSDTGWAKsAWSSVFAPWI 296 zebrafish
P0C7M7       208 FASEEHSCVETgsQEPMTIYFTSGTTGFPKMAQHSQSSLGIGFTLCGRyWLDLKSSDIIWNMSDTGWVKaAIGSVFSSWL 287 human
Q6NUN0       209 EASTEHNCMRTksRDPLAIYFTSGTTGAPKMVEHSQSSYGLGFVASGRrWVALTESDIFWNTTDTGWVK-AAWTLFSAWP 287 human
Feature 1                    #                   # #   #                   ####                  
3EQ6_A       272 lGACTFVHLLPKFDPLVILKTLSSYPIKSMMGAPIVYRMLLQQDLsSYKFPHLQNCVTVGESLLPETLENWRaQTGLDIR 351 human
2WD9_A       272 lGACTFVHLLPKFDPLVILKTLSSYPIKSMMGAPIVYRMLLQQDLsSYKFPHLQNCVTVGESLLPETLENWRaQTGLDIR 351 human
3B7W_A       272 lGACTFVHLLPKFDPLVILKTLSSYPIKSMMGAPIVYRMLLQQDLsSYKFPHLQNCVTVGESLLPETLENWRaQTGLDIR 351 human
3C5E_A       272 lGACTFVHLLPKFDPLVILKTLSSYPIKSMMGAPIVYRMLLQQDLsSYKFPHLQNCVTVGESLLPETLENWRaQTGLDIR 351 human
Q7TN78       288 rGACVFVHRMAQFNTDTFLDTLTSYPITTLCSAPTVYRMLVQQDLkRYQFKRLRHCLTGGEPLNPEVLEQWKaQTGLELY 367 Norway rat
Q08AH1       285 aGCTVFIHHLPQFDTKVIIQTLLKYPINHFWGVSSIYRMILQQDFtSIRFPALEHCYTGGEVVLPKDQEEWKrRTGLLLY 364 human
NP_001086370 289 qGSCVFAHSMPRFDPNIVLQTLSTFPITTFCSAPTAYRIFVLQNLaSYQFKSLQHCVSAGEPINPQVMEQWKeQTGLDIY 368 African clawed ...
Q53FZ2       294 qGACVFTHHLPRFEPTSILQTLSKYPITVFCSAPTVYRMLVQNDItSYKFKSLKHCVSAGEPITPDVTEKWRnKTGLDIY 373 human
NP_001104706 297 qGACVFVHHMPRFDTNTVLKTLSHYPITTFCTAPTAYRMLVQDDLsKYKFQALEHCLCAGEPINPEVMCKWKeLTGLDIY 376 zebrafish
P0C7M7       288 cGACVFVHRMAQFDTDTFLDTLTTYPITTLCSPPTVYRMLVQKDLkRYKFKSLRHCLTGGEPLNPEVLEQWRvQTGLELY 367 human
Q6NUN0       288 nGSCIFVHELPRVDAKVILNTLSKFPITTLCCVPTIFRLLVQEDLtRYQFQSLRHCLTGGEALNPDVREKWKhQTGVELY 367 human
Feature 1        ######  ##              #                                                       
3EQ6_A       352 ESYGQTETGLTCMVSKTMKIKPGYMGTAASCYDVQIIDDKGNVLPPGTEGDIGIRVKPIRPIGIFSGYVDNPDKTAANIR 431 human
2WD9_A       352 ESYGQTETGLTCMVSKTMKIKPGYMGTAASCYDVQIIDDKGNVLPPGTEGDIGIRVKPIRPIGIFSGYVDNPDKTAANIR 431 human
3B7W_A       352 ESYGQTETGLTCMVSKTMKIKPGYMGTAASCYDVQIIDDKGNVLPPGTEGDIGIRVKPIRPIGIFSGYVDNPDKTAANIR 431 human
3C5E_A       352 ESYGQTETGLTCMVSKTMKIKPGYMGTAASCYDVQIIDDKGNVLPPGTEGDIGIRVKPIRPIGIFSGYVDNPDKTAANIR 431 human
Q7TN78       368 EGYGQTEVGIICANRKGEEIKPGSMGKGVVPYDVQIIDEHGNILPSGKEGEIALRLGSDRPFCFFSEYVDNPEKTDATIR 447 Norway rat
Q08AH1       365 ENYGQSETGLICATYWGMKIKPGFMGKATPPYDVQVIDDKGSILPPNTEGNIGIRIKPVRPVSLFMCYEGDPEKTAKVEC 444 human
NP_001086370 369 EGYGQTETVLICGTFKGMKIKPGSMGKPSPAYDVQIIDENGDVLPQGKEGDIAIRVSPQKPFYLFSQYTGDPERTASTRR 448 African clawed ...
Q53FZ2       374 EGYGQTETVLICGNFKGMKIKPGSMGKPSPAFDVKIVDVNGNVLPPGQEGDIGIQVLPNRPFGLFTHYVDNPSKTASTLR 453 human
NP_001104706 377 EGYGQTETVLIAGTFKGMKIKPGSFGKASPGYDVQVVDENGSVVPKGQEGDLGIRVKPERPFSLFTEYTGEPVRTAECFR 456 zebrafish
P0C7M7       368 EGYGQTEVGMICANQKGQEIKPGSMGKGMLPYDVQIIDENGNVLPPGKEGEIALRLKPTRPFCFFSKYVDNPQKTAATIR 447 human
Q6NUN0       368 EGYGQSETVVICANPKGMKIKSGSMGKASPPYDVQIVDDEGNVLPPGEEGNVAVRIRPTRPFCFFNCYLDNPEKTAASEQ 447 human
Feature 1               #           #  #     # ####                            #                 
3EQ6_A       432 GDFWLLGDRGIKDEDGYFQFMGRADDIINSSGYRIGPSEVENALMEHPAVVETAVISSPDPVRGEVVKAFVVLASQFlSH 511 human
2WD9_A       432 GDFWLLGDRGIKDEDGYFQFMGRADDIINSSGYRIGPSEVENALMEHPAVVETAVISSPDPVRGEVVKAFVVLASQFlSH 511 human
3B7W_A       432 GDFWLLGDRGIKDEDGYFQFMGRADDIINSSGYRIGPSEVENALMEHPAVVETAVISSPDPVRGEVVKAFVVLASQFlSH 511 human
3C5E_A       432 GDFWLLGDRGIKDEDGYFQFMGRADDIINSSGYRIGPSEVENALMEHPAVVETAVISSPDPVRGEVVKAFVVLASQFlSH 511 human
Q7TN78       448 RNFYITGDRGVMDDDGYLWFVGRADDVIISSGYRIGPFEVESALIEHPAVVESAVVSSPDPIRGEVVKAFIVLAAPFkSS 527 Norway rat
Q08AH1       445 GDFYNTGDRGKMDEEGYICFLGRSDDIINASGYRIGPAEVESALVEHPAVAESAVVGSPDPIRGEVVKAFIVLTPQFlSH 524 human
NP_001086370 449 GNFYLTGDRGIKDEDGYFWFVGRSDDVILSSGYRIGPFEIESALIEHPAVAESAVVSSPDPIRGEVVKAFVVLAPAYnGH 528 African clawed ...
Q53FZ2       454 GNFYITGDRGYMDKDGYFWFVARADDVILSSGYRIGPFEVENALNEHPSVAESAVVSSPDPIRGEVVKAFVVLNPDYkSH 533 human
NP_001104706 457 GDFYLTGDRGMMDDEGYLWFIGRSDDVILSAGYRIGPFEVENALIEHPAVAESAVVSSPDPVRGEVVKAFVVLTADFkSR 536 zebrafish
P0C7M7       448 GDFYVTGDRGVMDSDGYFWFVGRADDVIISSGYRIGPFEVESALIEHPAVVESAVVSSPDQIRGEVVKAFVVLAAPFkSY 527 human
Q6NUN0       448 GDFYITGDRARMDKDGYFWFMGRNDDVINSSSYRIGPVEVESALAEHPAVLESAVVSSPDPIRGEVVKAFIVLTPAYsSH 527 human
Feature 1                     #    ## # #                          
3EQ6_A       512 DPEQLTKELQQHVKSVTAPYKYPRKIEFVLNLPKTVTGKIQRAKLRDKEW 561 human
2WD9_A       512 DPEQLTKELQQHVKSVTAPYKYPRKIEFVLNLPKTVTGKIQRAKLRDKEW 561 human
3B7W_A       512 DPEQLTKELQQHVKSVTAPYKYPRKIEFVLNLPKTVTGKIQRAKLRDKEW 561 human
3C5E_A       512 DPEQLTKELQQHVKSVTAPYKYPRKIEFVLNLPKTVTGKIQRAKLRDKEW 561 human
Q7TN78       528 NREKLTAELQDHVKNSTAPYKYPRKVEFVQELPKTITGKIKRNVLRDQEW 577 Norway rat
Q08AH1       525 DKDQLTKELQQHVKSVTAPYKYPRKVEFVSELPKTITGKIERKELRKKET 574 human
NP_001086370 529 DPEKLTLELQEHVKNITAPYKYPRKIEFVQQLPKTVSGKIRRNELRNREW 578 African clawed frog
Q53FZ2       534 DQEQLIKEIQEHVKKTTAPYKYPRKVEFIQELPKTISGKTKRNELRKKEW 583 human
NP_001104706 537 AHKELIKELQTHVKSITAPYKYPRKIEFVDQLPKTVSGKIRRVELRKKEW 586 zebrafish
P0C7M7       528 NPEKLTLELQDHVKKSTAPYKYPRKVEFVQELPKTITGKIKRNVLRDQEW 577 human
Q6NUN0       528 DPEALTRELQEHVKRVTAPYKYPRKVAFVSELPKTVSGKIQRSKLRSQEW 577 human

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