1P9E,4LE6,4O98


Conserved Protein Domain Family
OPHC2-like_MBL-fold
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cd07720: OPHC2-like_MBL-fold 
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Pseudomonas pseudoalcaligenes organophosphorus hydrolase C2, and related proteins; MBL-fold metallo hydrolase domain
Pseudomonas pseudoalcaligenes OPHC2 is a thermostable organophosphorus hydrolase which a broad substrate activity spectrum: it hydrolyzes various phosphotriesters, esters, and a lactone. This subgroup also includes Pseudomonas oleovorans PoOPH which exhibits high lactonase and esterase activities, and latent PTE activity. However, double mutations His250Ile/Ile263Trp switch PoOPH into an efficient and thermostable PTE. Members of this subgroup belong to the MBL-fold metallo-hydrolase superfamily which is comprised mainly of hydrolytic enzymes which carry out a variety of biological functions.
Links
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Statistics
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PSSM-Id: 293806
Aligned: 229 rows
Threshold Bit Score: 183.134
Created: 20-May-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 12 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Comment:includes metal binding residues and conserved mainly hydrophobic resides of substrate binding pocket
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                    #                                                                
1P9E_A     50 MLLGDFEITALSDGTVALpvdkrlnqpapkt--qsalaksfqkaplETSVTGYLVNTG-SKLVLVDTGAAglfgptlGRL 126 Pseudomonas sp. WBC-3
CCY34575   21 SGKSETEIIWLCDKAAGQsltlfpdaspe------lvtslglkeavPSSISAFLVRKD-GKVLLFDTGLGlp----eGGI 89  Alistipes sp. CAG:831
EIP98445   51 RKMGDVDFFSLLDRSSEFtreifigtnsr------llerlapqnriAASINAFVIRSG-ETTVLVDTGMG-------FGV 116 Opitutaceae bacter...
4LE6_A     40 MALGDFEVTALYDGYVDLpasllkgiddkdlqsllarmfvasekgvQTAVNAYLINTG-DNLVLIDTGAAqcfgptlGVV 118 Pseudomonas pseudo...
ETK04950   38 TIGDGVTVTWIRDNAADHtmarslfvdtpd----svmarlgltdgvPATISTFLVETG-GRIILFDTGYGfp----dSRL 108 Tannerella sp. ora...
ACX73930   59 TLANGAVVTWIQDNEGKKfmprelfsdasd----slyeslnmpaglPASVSTFLVKTD-GKYILFDAGLGaw----gGQL 129 Fibrobacter succin...
CCY03230   31 PVNESTTITWIKDKPGPTlqphklypdvpd----slwnalglqngvPASINCFLLQTE-GKNILLDTGLGap----vSQL 101 Prevotella sp. CAG...
ACC97851   28 YDIGSYKFYAMINNTGAIsdvlignkdki------tfdkykaagwnANAINYFLLDTG-TQKILFDTGLPla---rgGKV 97  Elusimicrobium min...
CDB54406   26 FQFGNFEVIAVQDALSAMrfelfpgmpee------eflrltgggrvPSSVNVFLLKRGdGKIILVDAGNGgk----rGGM 95  Akkermansia mucini...
CCY15257   58 LELGDVRVTWIQDQAAMQpvnlfpqapas------lidslhlrggiPSSLSTFLVESK-GMRILFDTGLGgk----dSRL 126 Prevotella sp. CAG...

Feature 1                         # # ##                               #                      
1P9E_A    127 AANLKAAGYQPEQVDEIYITHMHPDHVGGLMVGEQl-----aFPNAVVRADQKEADFWlsqtnldkapddeskgffkgam 201 Pseudomonas sp. WBC-3
CCY34575   90 RRGLDSLGLSPDDVDYLFLTHFHGDHIGGMLGPDGap----vYTNAQVYASRPEYDAWmnmpadrnag------------ 153 Alistipes sp. CAG:831
EIP98445  117 LDHLKAAGIRPEQINAVLLTHSHQDHVQGLLAGQEnr---atFPNATLWIAPAEMEFWksanatwfe------------- 180 Opitutaceae bacter...
4LE6_A    119 QTNLKASGYQPEQVDTVLLTHLHPDHACGLVNADGsp----aYPNATVEVPQAEAEFWldeatmakapegmqg-mfkmaq 193 Pseudomonas pseudo...
ETK04950  109 ADALRRLGHDPSEIDLVYLTHFHRDHIGGLMHGDTa-----aFPHAEVYASRPEYDAWmamppdrnaq------------ 171 Tannerella sp. ora...
ACX73930  130 LKRLDALKVNPDSIGLVYLTHFHADHIAGLVKSGGtgkmekvFKNASVYAGKVEYDAWmnnipkndl------------- 196 Fibrobacter succin...
CCY03230  102 LPKLKEQGLSPEDIGLIYLSHLHYDHIGGLLKDGAv-----tFPNAELYVNRVEAEAWlamndd---------------- 160 Prevotella sp. CAG...
ACC97851   98 LERLEAIGVKPQDISIICLTHMHMDHIGGMLDDEGkk----vFPNAKVFLSKEEAEYWnnpnndsssfk----------s 163 Elusimicrobium min...
CDB54406   96 LRKLEQSGVFPESVDFILLTHMHGDHIGGLLGKHGea----vFSRALVYVSMPEWEFWqngtagpqgk-----------q 160 Akkermansia mucini...
CCY15257  127 LHLLSLMELTPADIGFIYLTHLHSDHIGGLLDGGRp-----mFPMAQVFVSKQEFKGWmgmp------------------ 183 Prevotella sp. CAG...

Feature 1                                                 #                    #  #           
1P9E_A    202 aslnpyvkaGKFKPFSg-----------NTDLV-PGIKALASHGHTPGHTTYVVEsqgqKLALLGDLILVAAVQFddPSV 269 Pseudomonas sp. WBC-3
CCY34575  154 avrtmnaysDRLHLFE------------FGDILpCGVESLYAVGHTPGHTVFRVG----DILIWGDIIHGLALQLdhPEI 217 Alistipes sp. CAG:831
EIP98445  181 -kcrqayrnWKMIPNDk----------tAPIVL-PQITAIAAPGHTPGHTVFLVStkdeRLLLAGDLLHGVPLQFldPEV 248 Opitutaceae bacter...
4LE6_A    194 qavapyakmNKLKPYKt-----------EGELL-PGVSLVASPGHTPGHTSYLFKsggqSLLVWGDILLNHAVQFakPEV 261 Pseudomonas pseudo...
ETK04950  172 qvkimnayrDRLHLFAf-----------GDTLP-GGVLTIDAVGHTPGHTAYRVG----RLLIIGDLIHGAALQLerPEY 235 Tannerella sp. ora...
ACX73930  197 qknimalykDSLHLFAf-----------GDSLP-HGVLAMDAVGHTPGHTAFKIS----NLLVIGDLMHGYALQKnhPEI 260 Fibrobacter succin...
CCY03230  161 ---nsklakEVLKAYKkhlh----lfeaGDTLQ-GGVKTIAAYGHTPGHTLFQKD----SILVIADLIHGAALQLkhPEY 228 Prevotella sp. CAG...
ACC97851  164 akkvlevygESIIKFPq-----------KQKIT-DGVTSVPLFGHTPGHTGFIIEnkgkKLVILGDVIHANIQFVn-PDI 230 Elusimicrobium min...
CDB54406  161 vrkvlhaygSRVRTFRf-----------GEEVL-PGIKALDASGHTPGHTVFETD----SLLIVGDLLHAAALQIprPEV 224 Akkermansia mucini...
CCY15257  184 -----gekkAQVEKMAkayrlnlhffdfGDYLP-GGVETIDASGHTPGHTAFRVG----DLLIVGDILHGAALQLanLDL 253 Prevotella sp. CAG...

Feature 1      # #                            #
1P9E_A    270 TTQLDsDSKSVAVERKKAFADAAkgGYLIAASH 302 Pseudomonas sp. WBC-3
CCY34575  218 CATYDmDKEAAIAARRHMIRIAReqHLLVAGMH 250 Alistipes sp. CAG:831
EIP98445  249 NARFDvDQKMAAKTRRQILSRVAanGWTFAGAH 281 Opitutaceae bacterium TAV1
4LE6_A    262 VFEFDvDSDQARQSRQRILAEAAtdKLWVAGAH 294 Pseudomonas pseudoalcaligenes
ETK04950  236 STSYDmDRPAAARTRRRILDYARreGLVMAGMH 268 Tannerella sp. oral taxon BU063 isolate Cell 5
ACX73930  261 NSNYDmDKEKSAASRKRIMQYARenKLLMAGMH 293 Fibrobacter succinogenes subsp. succinogenes S85
CCY03230  229 CPTYDmDPKAARESRIRILKYAKdnHLTMYGMH 261 Prevotella sp. CAG:924
ACC97851  231 YLTYDvDPKTAMKTRKLVLKKAAaeGVDITGMH 263 Elusimicrobium minutum Pei191
CDB54406  225 CTIYDmNPSGAVQARRRFYEFAAssPKPVAGMH 257 Akkermansia muciniphila CAG:154
CCY15257  254 CPTYDmNPAEARVARKRILQLARtdSLVMAGMH 286 Prevotella sp. CAG:755

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