2HYK,3DGT,2VY0,2W39,2W52,2CL2


Conserved Protein Domain Family
GH16_laminarinase_like
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cd08023: GH16_laminarinase_like 
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Laminarinase, member of the glycosyl hydrolase family 16
Laminarinase, also known as glucan endo-1,3-beta-D-glucosidase, is a glycosyl hydrolase family 16 member that hydrolyzes 1,3-beta-D-glucosidic linkages in 1,3-beta-D-glucans such as laminarins, curdlans, paramylons, and pachymans, with very limited action on mixed-link (1,3-1,4-)-beta-D-glucans.
Links
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Statistics
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PSSM-Id: 185693
Aligned: 165 rows
Threshold Bit Score: 100.777
Created: 30-Sep-2009
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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active sitecatalytic
Conserved site includes 7 residues -Click on image for an interactive view with Cn3D
Feature 1:active site [active site]
Evidence:
  • Structure:2W52; Phanerochaete chrysosporium Laminarinase 16A with bound 2 beta-glucans in both donor and acceptor sites, contact 4 A
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                                        
2HYK_A        11 WSDEFDgpags--apdpaNWNHetgdhgwgnn-------elqnytdsraNSALdgnGNLVITARqead--------ggyT 73  Nocardiopsis sp...
2W39_A         7 DNWVGSaf--------lsTFTHeaiadpthgrvn----yvdqatalaknLTYAs-gDTLILRADhtttls---psgpgrN 70  Phanerochaete c...
2W52_A         7 DNWVGSaf--------lsTFTHeaiadpthgrvn----yvdqatalaknLTYAs-gDTLILRADhtttls---psgpgrN 70  Phanerochaete c...
2CL2_A         7 DNWVGSaf--------lsTFTHeaiadpthgrvn----yvdqatalaknLTYAs-gDTLILRADhtttls---psgpgrN 70  Phanerochaete c...
ZP_02026685  200 WSDEFNgssl-----dmnNWTYetgtgdggwgn------qewqtytagdNAKVe-nGNLVIIPRmewkngn--napskvT 265 Eubacterium ven...
ZP_01464833  132 WEDTFStngq----pnaaNWSYhagngfnpgnqsfsgwgngewewyrpsNTYVq-dGNLVIRADynktatrlqnrdwfqF 206 Stigmatella aur...
XP_001019431  57 FRDDFNgtkl-----nttIWTArnnmthgsw----------eqqlyqsdDVYVe-nGNLVLRTRfnpqkyipsgrlynyT 120 Tetrahymena the...
XP_001023512  35 FEDEFEgtqv-----desKWTKryrthgdt-----------eqqlyiedDIYTe-nGNLVIRTRynptvynltgklynfT 97  Tetrahymena the...
XP_001020896  40 FQDEFDgdqi-----dqsKWNIannfthtqk----------eqqiyfpeNAYTe-nGNLVIKTVfqptfyapfnrtynyT 103 Tetrahymena the...
P32486       330 FSDEFNaegrtfydgddpYWTApdvhydat-----------kdlewyspDASTtvnGTLQLRMDafknh------glyyR 392 baker's yeast

Feature 1          #                               #   #                                         
2HYK_A        74 SARLTTqnKVQPQy-GRVEASIQ-IPRg---qGIWPAFWMLGadfpnt-------------------------------- 116 Nocardiopsis sp...
2W39_A        71 SVRIRS--IKTYTt-HVAVFDVRhMPQg---cGTWPAAWETDegdw---------------------------------- 110 Phanerochaete c...
2W52_A        71 SVRIRS--IKTYTt-HVAVFDVRhMPQg---cGTWPAAWETDegdw---------------------------------- 110 Phanerochaete c...
2CL2_A        71 SVRIRS--IKTYTt-HVAVFDVRhMPQg---cGTWPAAWETDegdw---------------------------------- 110 Phanerochaete c...
ZP_02026685  266 STRIITknKKTFKy-GKMEIRAK-AAGg---kGTWSAGWMLGdgtgdq-------------------------------- 308 Eubacterium ven...
ZP_01464833  207 SGRITTkgKKSWKy-GRVEARIA-MPSa---vGTWPAFWMMGtacddtvtttytpa------------------------ 257 Stigmatella aur...
XP_001019431 121 SGWVDSqgKFSYKy-GYAEARIL-FSKivkndPIWPAFWSIGenty---------------------------------- 164 Tetrahymena the...
XP_001023512  98 SGWLDSsqKFNFSe-GRLEARVK-LPKtkvndPIWPALWTIGdgvs---------------------------------- 141 Tetrahymena the...
XP_001020896 104 SAWLDSskKFNFTsgSKIEARIL-LPKtskgdQIWPAFWTKGelap---------------------------------- 148 Tetrahymena the...
P32486       393 SGMLQSwnKVCFTq-GALEISAN-LPNygrvsGLWPGLWTMGnlgrpgylastqgvwpysyescdagitpnqsspdgisy 470 baker's yeast

Feature 1                                  # #  #                 #                              
2HYK_A       117 --------------------pwpdsGEIDIMENIGre----phLVHGSLHgpgyf----------------------gge 150 Nocardiopsis sp...
2W39_A       111 ----------------------pngGEVDIIEGVNdq-----sPNAMTLHtgancampasrtmtghatnnncdvntdgnt 163 Phanerochaete c...
2W52_A       111 ----------------------pngGEVDIIEGVNdq-----sPNAMTLHtgancampasrtmtghatnnncdvntdgnt 163 Phanerochaete c...
2CL2_A       111 ----------------------pngGEVDIIEGVNdq-----sPNAMTLHtgancampasrtmtghatnnncdvntdgnt 163 Phanerochaete c...
ZP_02026685  309 -------------------rgwpydGEIDIMEAMSg-------GVPQTIHcerfn----------------------nqs 340 Eubacterium ven...
ZP_01464833  258 -----------adrydtmasnwsscGEIDIMEHKNte-----tRTFQNVFwdsrtglfp--------------wadgqnn 307 Stigmatella aur...
XP_001019431 165 ---------------------wpngGEIDTMEMSTqwgtngnpKIWGTYHwgktd----------------------qcn 201 Tetrahymena the...
XP_001023512 142 ---------------------wlrgGEIDIFEMNSnwngdklpHLYGTYHwgnnas-------------------dnhch 181 Tetrahymena the...
XP_001020896 149 ---------------------wpsgGEIDIMEMSTnwygdnlpHLYGTYHwgqpge--------------------dycg 187 Tetrahymena the...
P32486       471 lpgqklsictcdgedhpnqgvgrgaPEIDVLEGETdtki-gvgIASQSLQiapfdiwympdydf---ievynfttttmnt 546 baker's yeast

Feature 1                                                                                        
2HYK_A       151 pLTGSYmhpqgw------------sfadtFHTFAVDWrp----gSITWSVD-----------------------GVAYQT 191 Nocardiopsis sp...
2W39_A       164 gCGVQAptansyg---------psfnangGGWYAMERtn----sFIKVWFFprnagnvpndiasgpatintdnwGTPTAF 230 Phanerochaete c...
2W52_A       164 gCGVQAptansyg---------psfnangGGWYAMERtn----sFIKVWFFprnagnvpndiasgpatintdnwGTPTAF 230 Phanerochaete c...
2CL2_A       164 gCGVQAptansyg---------psfnangGGWYAMERtn----sFIKVWFFprnagnvpndiasgpatintdnwGTPTAF 230 Phanerochaete c...
ZP_02026685  341 wSHGNKnyatglt---------qaksaetYHTYGIIWtd----kYIQFTVD-----------------------GVNKGL 384 Eubacterium ven...
ZP_01464833  308 eQPSNInvgn----------------vtqFHLYSIEWep----tQIRWYVD-----------------------RETNPS 344 Stigmatella aur...
XP_001019431 202 gCGSQFtgnl---------------dfseYHTYTTVWne----tQIYWYVD-----------------------GIHYKT 239 Tetrahymena the...
XP_001023512 182 gCGTLYpnki---------------nlndYHIFRFDWna----tQFDWYID-----------------------NIHYKT 219 Tetrahymena the...
XP_001020896 188 gCGTIFpdfql--------------nldeYINFSIIWdd----nQITWINQ-----------------------QGVFYN 226 Tetrahymena the...
P32486       547 yAGGPFqqavsavstlnvtwyefgeyggyFQKYAIEYlndddngYIRWFVG-----------------------DTPTYT 603 baker's yeast

Feature 1                                                                                  
2HYK_A       192 YTsadtrg-----npwvfdQPFFMILNVAVGgdwpgyp------------------dgstqfPQEMRVDYVRVY 242 Nocardiopsis sp. F96
2W39_A       231 FPntncd-------igshfDANNIIINLTFCgdwagqasifngagcpgscvdyvnnnpsafaNAYWDIASVRVY 297 Phanerochaete chrysos...
2W52_A       231 FPntncd-------igshfDANNIIINLTFCgdwagqasifngagcpgscvdyvnnnpsafaNAYWDIASVRVY 297 Phanerochaete chrysos...
2CL2_A       231 FPntncd-------igshfDANNIIINLTFCgdwagqasifngagcpgscvdyvnnnpsafaNAYWDIASVRVY 297 Phanerochaete chrysos...
ZP_02026685  385 YDpsmydasi-ydqcwafdHPFFFILNCAVGgnaagevstdgw-------tnkgtvngvttwEDYYYVDYVRVY 450 Eubacterium ventriosu...
ZP_01464833  345 PVhtvdita---gnkeefqKPFHIILNLALSgiftgyl-----------------epnpadfPMYMKVDYVRVW 398 Stigmatella aurantiac...
XP_001019431 240 NVngttypnt-ksplilpnYSQYFIINDALWgtpep---------------------npsdyPKYMYIDYVRVL 291 Tetrahymena thermophila
XP_001023512 220 NKqgvqypkt-dapitlptYPQFIIINTAIDnfika---------------------kqadyPKYMYIDYIRVY 271 Tetrahymena thermophila
XP_001020896 227 NTsvhep-------idlpqFPQYLIINDAIDgfatp---------------------nqaeyPKYMKIDYVRAY 272 Tetrahymena thermophila
P32486       604 IHakalhpdgnigwrriskEPMSIILNLGISnnwayid------------------wqyiffPVVMSIDYVRIY 659 baker's yeast

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