6D6I,1JAT,2Q0V


Conserved Protein Domain Family
UEV_UBE2V

?
cd23807: UEV_UBE2V 
ubiquitin E2 variant (UEV) domain of ubiquitin-conjugating enzyme E2 variant 1, 2 and related proteins
The E2V subfamily includes mammalian ubiquitin-conjugating enzyme E2 variant 1 (UBE2V1/CROC1/UBE2V/UEV1) and variant 2 (UBE2V2/MMS2/UEV2), yeast ubiquitin-conjugating enzyme variant MMS2, plant ubiquitin-conjugating enzyme E2 variant 1A (UEV1A/MMZ1) and variant 1B (UEV1B/MMZ2), and similar proteins. They have no ubiquitin ligase activity on their own. UBE2V1 (also called UEV-1, CROC-1, or TRAF6-regulated IKK activator 1 beta Uev1A) and UBE2V2 (also called DDVit 1, enterocyte differentiation-associated factor 1 (EDAF-1), enterocyte differentiation-promoting factor 1 (EDPF-1), MMS2 homolog, or vitamin D3-inducible protein) form heterodimers with UBE2N, respectively. Saccharomyces cerevisiae UEV MMS2 has a role in the DNA error-free postreplication repair (PRR) pathway. It forms a heterodimer with UBC13. Arabidopsis thaliana UEV1A and UEV1A form heterodimers with UBC35 or UBC36. The heterodimers possess ubiquitin-conjugating enzyme (EC 2.3.2.23) activity, catalyzing the synthesis of non-canonical poly-ubiquitin chains that are linked through 'Lys-63'. This type of poly-ubiquitination does not lead to protein degradation by the proteasome. Members of this subfamily may mediate transcriptional activation of target genes and plays a role in the control of progress through the cell cycle and differentiation. UEVs are homologous to E2 ubiquitin ligases but lack the conserved cysteine residue required for catalytic activity.
Statistics
?
PSSM-Id: 467427
Aligned: 72 rows
Threshold Bit Score: 187.309
Created: 11-Aug-2017
Updated: 27-Apr-2023
Structure
?
Program:
Drawing:
Aligned Rows:
 
Conserved site includes 13 residues -Click on image for an interactive view with Cn3D
Feature 1:UBE2N interaction site [polypeptide binding site]
Evidence:
  • Structure:6D6I: human UBE2V1 binds UBE2N, contacts at 4A
  • Comment:UBE2V1 lacks an active site cysteine but works with the E2 enzyme Ube2N to facilitate Ub chain formation by binding and positioning Lys63 of the acceptor Ub for isopeptide bond formation with the charged Ub on Ube2N

Sequence Alignment
?
Format: Row Display: Color Bits: Type Selection:
Feature 1            # ##  ## ##  #            #  ## # #                                          
6D6I_A          5 VKVPRNFRLLEELEEGQKGVGd-GTVSWGLEDDEdMTLTRWTGMIIGPPrtiyeNRIYSLKIECGpKYPEaPPFVRFVTK 83   human
XP_002907974 1193 VIVPRNFKLLEELENSEKGHGd-MSISYGLEQADdIFLTNWIGTILGPAgtchdGRIYSLRIHCSdQYPHvPPEVHFTSR 1271 Phytophthora ...
EOD15369        9 VIVPRNFVLLDELDKSEKAVGd-MTCSYGLVQPDdIWLNHWNGSILGPTssqveGRILQLLIFCGkEYPKaAPQVKFISK 87   Emiliania hux...
XP_005837250    5 VIVPRNFVLLEELEKGEKGVGd-GSVSYGLADGNdNTLTTWNGMIVGPHgsqleGRILSLVITCGeRYPDeSPKVRFQSR 83   Guillardia th...
EWM25895       27 IIVPRNFKLLEELDQAEKGQD--PTISLGLQNQEdIFLVAWNGSIFGPPgttfeGRLFELKIECGdRYPNePPFVRFVSR 104  Nannochlorops...
OEU19841       10 VITPRNFRLLEELENSEKGHGd-MSISFGLVESGdTFLSDWNGGILGPAgtqhdGRFYQLLIHCTqEYPAvPPTIRFTSK 88   Fragilariopsi...
OHT10302        3 VEVPRNFRLLEELEVGEKGQDipPNISFGLMDNGdSTLSNWVGTIHGSPgtrfdSRMVSIKFYCGeNYPKqPPTVSFITR 82   Tritrichomona...
KAA6393672      6 VVVPRNFRLLDELQEGEKLVSd-NRISFGAEGDD-PLLHNWSAMLYGPEntrfvGRLYSVTIYCGdNYPKePPTIQFTTK 83   Streblomastix...
XP_002291290   10 VIVPRNFKLLEELEHSEKGLGd-MSISYGLVDSSdIFMGEWNGGILGPHgtqhdSRFYELRISIPeSYPAmPPKVRFVSR 88   Thalassiosira...
XP_001578934    2 VEPPRNFRLLDELETGEKDQDmpAGISFGLEDYSdMTLTNWIGTIFGMPktcydGRVYTIKIVCGpDYPKvAPKVNFVTK 81   Trichomonas v...
Feature 1                                                                    
6D6I_A         84 INMNGVnsSNGVVDPRAIsv----lAKWQNSYsIKVVLQELRRLMMSKENmkLPQPPEG 138  human
XP_002907974 1272 INMSCVdpQSGRVDPRRLaa----lGSWHRNNgIEHVLVAIRAEMASPTNrrLPQPPEG 1326 Phytophthora infestans T30-4
EOD15369       88 VNFPGIvsDTGVVDVPKVlskvgasRQWDSKMrIETILKDIKKALTLPEYrkVLQPQDG 146  Emiliania huxleyi CCMP1516
XP_005837250   84 VNLNFVdqHNGAVDSRSVsi----lSHWNRSCtMEKLLQELRREMCSSHNrrLPQPPDG 138  Guillardia theta CCMP2712
EWM25895      105 VNLPCVdqSTGVVNPSKLhi----lSHWDRHFtMERVLNELRREMMTQGSrrLPQPPEG 159  Nannochloropsis gaditana
OEU19841       89 INMSCIdsKTGVVEKNKVpa----mKNWNRNTgIEKVLQSIRMEMCSDKNrrLKQPAEG 143  Fragilariopsis cylindrus CCMP1102
OHT10302       83 VNLPFVd-GNGNIIINNFpl----lKDWRPATtILVILIEISNLMKKYGN--LQQPPDG 134  Tritrichomonas foetus
KAA6393672     84 INLPCVd-AQGRVIRDRVpl----lKNWEYNNtIQKSLLALHEQMVLNKQ--LPQPAEG 135  Streblomastix strix
XP_002291290   89 VNMNCVdpKSGEVIYSKVpa----tKNWNRNMgMEQILISLRAEMASGENrrLKQPLEG 143  Thalassiosira pseudonana CCMP1335
XP_001578934   82 INLPFVd-KNGHIKPAQFpl----lGNWNSSTtILKILTEIYETMKKNPQ--LPQPSDG 133  Trichomonas vaginalis G3

| Disclaimer | Privacy statement | Accessibility |
NCBI Home NCBI Search NCBI SiteMap