1H0A,3ONK,4GZC,2QY7,5J08


Conserved Protein Domain Family
ENTH

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cd03571: ENTH 
Click on image for an interactive view with Cn3D
Epsin N-Terminal Homology (ENTH) domain family
The Epsin N-Terminal Homology (ENTH) domain is an evolutionarily conserved protein module found primarily in proteins that participate in clathrin-mediated endocytosis. ENTH domain is highly similar to the N-terminal region of the AP180 N-Terminal Homology (ANTH_N) domain. ENTH and ANTH_N domains are structurally similar to the VHS domain and are composed of a superhelix of eight alpha helices. ENTH domains bind both, inositol phospholipids with preference for PtdIns(4,5)P2, and proteins, contributing to the nucleation and formation of clathrin coats on membranes. ENTH domains also function in the development of membrane curvature through lipid remodeling during the formation of clathrin-coated vesicles. ENTH and ANTH (E/ANTH)-containing proteins have recently been shown to function with adaptor protein-1 and GGA adaptors at the Trans-Golgi Network, which suggests that E/ANTH domains are universal components of the machinery for clathrin-mediated membrane budding.
Statistics
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PSSM-Id: 340772
Aligned: 87 rows
Threshold Bit Score: 84.8765
Created: 2-Mar-2006
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
phosphoinosit..
Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1:phosphoinositide binding site [chemical binding site]
Evidence:
  • Structure:1H0A; Rattus norvegicus Epsin ENTH domain binds inositol 1,4,5-trisphosphate or Ins(1,4,5)p3; contacts at 4.0A
  • Comment:ENTH domains bind phosphoinositides (PtdInsPs), usually with a preference for PdtIns(4,5)P2, although not all ENTH domains show this preference.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1          #   ##                                         #                #   #      
1H0A_A     20 AEIKVREATSNdpwGPSSSLMSEIADLTYnv---------vAFSEIMSMIWKRLNdh-----------gKNWRHVYKAMT 79  Norway rat
CDW71230    4 YQKVLKECTSNenwNISNSKLQVLADHSYnw---------nDYTVIMQHLWKKMNsk-----------pKEWRRIFKALH 63  Stylonychia lemnae
CAK79977   22 LEQTLSEATSNenwNTPTKLLQEVAEASYgy---------tSCDTIMKFIWKRLDsd-----------nKEWRRILKTLN 81  Paramecium tetraur...
ETW59926   24 FEKNLKEALNNknyGVSNSLLYDLSISTYdv---------nYYKRVMTEVFKAIQek-----------pTRWRRIYKGLK 83  Plasmodium falcipa...
EAS03987   23 LEKLLHEVTSNdnwGVNPKLLRQVSDESYhf---------eNREVILNFIFHRLSnk-----------tKNWRKILKTLE 82  Tetrahymena thermo...
EJY64472   29 IDKILIEATGNenwNIANSKLQILSEACYhh---------dQCQKILDHLKSKLDsp-----------vFEWRRILKSLH 88  Oxytricha trifallax
CDW78484   29 IDKLLVEATANenwNIANSKLQILADACNspqllvlknnsdDCKVILDYLKYKLEvp-----------aYEWRRIMKSLS 97  Stylonychia lemnae
CDW83750   14 IEKILIEATGNenwNIANSKLQMLADAAHqh---------dNYQRIMDHLKGKLEcpafewrrvlkvskQKCLISMQSLV 84  Stylonychia lemnae
CCF72649   18 LDKLLKEALYDksvGCIDSILNGIAQASHhm---------pFYEIISKRTWSAINsk-----------pSKWLYIQKGLT 77  Babesia microti st...
CDR95405   19 VDKRIKEALYSndvGCPETVLYELSQATYha---------aFMERISQATWSCLKas-----------nARARRIQKALT 78  Babesia bigemina
Feature 1                                                                         
1H0A_A     80 LMEYLIKTGSERVSQQCKENMYAVqt--lkDFQYVDRDGk---------dQGVNVREKAKQLVALLRD 136 Norway rat
CDW71230   64 AMEYLIKNGAPRCIQEIKDDLFKIra--lqDFSYSENGTd----------RGQGVRDKSRSLCELLSD 119 Stylonychia lemnae
CAK79977   82 MIEYLTKNGAPRCVGEFRDNIYKIrs--fsDFFLVEQGSd----------KGLSIRDKTKQLVDLLSN 137 Paramecium tetraurelia strain ...
ETW59926   84 LCEYVMKNGCEYFISDVKDKEELIkk--ltHFTHLEDLKd----------KGIGIRDISNNILKLLKD 139 Plasmodium falciparum CAMP/Mal...
EAS03987   83 LIEYLIKNGLYDLCSDFRREIVHItt--hcDFQMLEKGVd----------RGLSIRNKAQKIVQLLSD 138 Tetrahymena thermophila SB210
EJY64472   89 ALEYIIKNGPPRLQGDLKYEMYKInn--lqNFTHFEEGAd----------KGSSIRQKSHQICDLLTN 144 Oxytricha trifallax
CDW78484   98 AMEYLLKNGPFRVQQDLRNEMFKVtt--lqNFTYYEDNId----------KGSSIREKAILLADLLTS 153 Stylonychia lemnae
CDW83750   85 ALEYILKHGPARVQQDLRNEMFRLnk--lqQFTHFEDGSd----------KGNSIRQKAHLIADLVTI 140 Stylonychia lemnae
CCF72649   78 LLEYLVLNGAEWCISDVLQHLEDInkvvklNREYSAKTSfltgkplapphTAFVITEKCKNLINLVRM 145 Babesia microti strain RI
CDR95405   79 LLTYLALNGAEGCASDILTRLDDIte--lhDRKLPEHMRd----------LEIIIKDKAINLVSLVCD 134 Babesia bigemina

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