Conserved Protein Domain Family
ATG14

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pfam10186: ATG14 
Vacuolar sorting 38 and autophagy-related subunit 14
The Atg14 or Apg14 proteins are hydrophilic proteins with a predicted molecular mass of 40.5 kDa, and have a coiled-coil motif at the N terminus region. Yeast cells with mutant Atg14 are defective not only in autophagy but also in sorting of carboxypeptidase Y (CPY), a vacuolar-soluble hydrolase, to the vacuole. Subcellular fractionation indicate that Apg14p and Apg6p are peripherally associated with a membrane structure(s). Apg14p was co-immunoprecipitated with Apg6p, suggesting that they form a stable protein complex. These results imply that Apg6/Vps30p has two distinct functions: in the autophagic process and in the vacuolar protein sorting pathway. Apg14p may be a component specifically required for the function of Apg6/Vps30p through the autophagic pathway. There are 17 auto-phagosomal component proteins which are categorized into six functional units, one of which is the AS-PI3K complex (Vps30/Atg6 and Atg14). The AS-PI3K complex and the Atg2-Atg18 complex are essential for nucleation, and the specific function of the AS-PI3K apparently is to produce phosphatidylinositol 3-phosphate (PtdIns(3)P) at the pre-autophagosomal structure (PAS). The localization of this complex at the PAS is controlled by Atg14. Autophagy mediates the cellular response to nutrient deprivation, protein aggregation, and pathogen invasion in humans, and malfunction of autophagy has been implicated in multiple human diseases including cancer. This effect seems to be mediated through direct interaction of the human Atg14 with Beclin 1 in the human phosphatidylinositol 3-kinase class III complex.
Statistics
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PSSM-Id: 462986
Aligned: 26 rows
Threshold Bit Score: 145.288
Created: 23-Mar-2022
Updated: 17-Oct-2022
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Q4WCL3         3 CPICSR-VSssrLRFYCPTC---ACN------------QIYTLRIDNARALLEKETLGRQVEKALLHQTsptlsyhrfeE 66  Aspergillus fum...
XP_002496378   3 CSICHK-SG---RPMYCAHC---MSSsp---------hMLLKLKLDLLILRETNGHLKSRVEDILEYGLgqi----agkE 62 
Q6FX35         5 CPICET-QS---HVFYCAHC---INSsp---------dLLIRLKFDLYILGKINNALRNKVDQYLEEVDdeln--tnirN 66  [Candida] glabr...
Q8H1E1         8 CAICDN-TN---RPCICTAC---VNH------------RLIEYNTLLKSLKTRRDSLLSRFNELLESKG----------- 57  thale cress
XP_006386388   8 CAICEN-SN---RASICPIC---VNY------------RLNEYGTLLKSLNSRRDSLYSKLSVVLIAKG----------- 57 
Q147F2         7 CAVCYK-SF---RPSICVDC---VNR------------GLNEYKVKLDSLKSLREVSYFRLSGLLVTKE----------- 56  thale cress
Q9VAP6        70 CPLCHScSA---SRFHCRNC---VRNgnithsqaerpeSLTEKQQRYINLQAGLKTFSTRYERLIGQHR----------S 133 fruit fly
Q6ZNE5        43 CPLCNT-TR---RRLTCAKC---VQSgdfvyfdgrdreRFIDKKERLSRLKSKQEEFQKEVLKAMEGKW----------I 105 human
EDO36555       3 CPLCLR-RS---KYLTCCSC---LRSgsvtht-gkrpeSYCEKVKRLADLKHVKEAFQKRVTEEIANRQ----------A 64 
Q54QH5         7 CCSCNN-RFn-fQESYCEPClqrIKNdq---------sRVFELIQSIFDKKKANLIAKNKILESISQPKn---------- 65  Dictyostelium d...
Q4WCL3        67 CsTQSPDKVPSPQDVLQIITAKAESSIR----------RKELALQIQQLKSEIKDRQLGISQRRLTLA------------ 124 Aspergillus fum...
XP_002496378  63 PkSASTNVEGDILGRRLRKLDSVILRRK----------NNRVKYRISQLNRRIYEKRHRIEALRSKL------------- 119
Q6FX35        67 GeQLESNIGVQILKERLGKVQVLRKERQ----------NNKIKHKISQQDRRIKEKSRYIAELRSLI------------- 123 [Candida] glabr...
Q8H1E1        58 -------------KADDQKNWRLIQNEK----------ISKLKKKLKSNKELVTQGKVKIERGSSDLK------------ 102 thale cress
XP_006386388  58 -------------KADDQFNWRVQQNEK----------LASSREKLHRNKEQLAQGKAKVEKLSQDLK------------ 102
Q147F2        57 -------------KAISQRCWKELQNEK----------LAKLREKLQLQVEKLQQSKNTFRSLSRNLK------------ 101 thale cress
Q9VAP6       134 NeDRLMAIKAKRKQLELLQQLISGTGQR----------LRVLGERRDELRRANAEKRKNLPKYPDKVKmlenyvldrvek 203 fruit fly
Q6ZNE5       106 TdQLRWKIMSCKMRIEQLKQTICKGNEEmeknsegllkTKEKNQKLYSRAQRHQEKKEKIQRHNRKLGdl---------- 175 human
EDO36555      65 VqQLNDAVILKREKISCIQSVIRLIQQH----------TSQDQQSLDSIRKSNAVKKVKLKSNADEIKtllevkkkhlat 134
Q54QH5        66 --QLQEQIDIKKTYLESKKIQIEILKNK----------IQTLKTTVIKDNGLIDVKKKYLSTRRSSLK------------ 121 Dictyostelium d...
Q4WCL3       125 --RRRSDAESSKFQLESREVALICGVQNTIK-RTERL------------WHSLHSKTVEA-----RIFLCREVANLYSLR 184 Aspergillus fum...
XP_002496378 120 --QLPSKIGIHEKELKAKRESQELTIS-----EGKQQ------------LTQIRKVLLKS-----QLSKIRSLVEWFVIR 175
Q6FX35       124 ----------NGPPKNQNRFTDPQTILKAQK-QLQDK------------LEIAKRLSIQT-----RSEKLAMLNKWYSIR 175 [Candida] glabr...
Q8H1E1       103 --VKYGVLDSARSTLEKTRVEQVEKYFPNLIcTQSLG------------HMAISSERLHK-----QSVVVKQICKLFPLR 163 thale cress
XP_006386388 103 --KKNGMLESARNVLEKNRMEQLEKFYPNLIcTQSLG------------HMAITSELLHK-----QSVVIKQICKLFPQR 163
Q147F2       102 --ERYGIIESTNVALEESRVHQLENHYSDTIgNHYLV------------YIDLTSERLYK-----QALVMKQICKLFPLS 162 thale cress
Q9VAP6       204 ieKLRETHLGLLDSIKQAARRSIQQLVTYVF-PIAEV---------------VLKEEQQRrasveRTEEAETIAALADAK 267 fruit fly
Q6ZNE5       176 veKKTIDLRSHYERLANLRRSHILELTSVIF-PIEEV------------KTGVRDPADVSse-sdSAMTSSTVSKLAEAR 241 human
EDO36555     135 lsKRMRDLKSLQNELGQVRQRSMALVQKYLF-PIQAIpvypvmgtppppQHTPESLDLSRelsfeDQADASVESALAEAT 213
Q54QH5       122 -------KNKISFEAVNNSKSGFSSLTDEIQ-KKKDQ------------LTAENTSLQIV-----KRSKIEQLTNSMTRL 176 Dictyostelium d...
Q4WCL3       185 Q-------WMKQdgSEMKETYVIGG--VPIVDLRDLNGksqncpllldm---papsinaqigATAVQISTSFSNIAHLLV 252 Aspergillus fum...
XP_002496378 176 Kr------------ESYEFPYTLAF--QPIVSLRNVYK------------------------LPSSVVWGSISTMSRYLK 217
Q6FX35       176 Kr------------DSHDIPFTISY--QPVISLKNFNR------------------------LPLPLIEGSLRKLIQYMN 217 [Candida] glabr...
Q8H1E1       164 Rvsf----dgeSqnGSVRQYDVICN--SRLPSGLDPHS------------------------IPSEELAVSLGYMVQLLN 213 thale cress
XP_006386388 164 Rvnvd------gerNFSGQYDQICN--ARLPRGLDPHS------------------------VSSEELAASLGYMVQLLN 211
Q147F2       163 Rvtv----egqNkdGSSVQYDQICN--ALLPQGLNPLS------------------------VPPKELAASLGYMVQLLN 212 thale cress
Q9VAP6       268 NtsyirgkWVFHgsGISEVQYRIVG--PSLPANGDYTAyldwladnkddvpkataneitpsrFEAYRIVGALTYTAQLTQ 345 fruit fly
Q6ZNE5       242 RttylsgrWVCDd-hNGDTSISITGpwISLPNNGDYSAyyswveekkt-----tqgpdmeqsNPAYTISAALCYATQLVN 315 human
EDO36555     214 QtsyvqgkWVn---ETATLELSIID--SSLPWSGDYSAyrhwvkahkng--skgpdddvglrNPAYTISSALTHAAQLLE 286
Q54QH5       177 Ql-----------nPTTPDHFFLFN--MVLNDQQLLK-------------------------FPKDVIFTTLGYISKIVV 218 Dictyostelium d...
Q4WCL3       253 LVSHYLSLRLPAEITLPHKDHPVPTIHAPIASYISRESILTATStq-------------------pHNLASSTTHPLGFQ 313 Aspergillus fum...
XP_002496378 218 LFAEILFFTLPHNELVAPNAP----------------------------------------------------------- 238
Q6FX35       218 LLEHIYCIKYPSAMFVHEDEM----------------------------------------------------------- 238 [Candida] glabr...
Q8H1E1       214 LVVHNLAAPALHSSGFAGSCSRIWQRDSYWDGRTSTRSNEYPLFiprrnycstsv-enswtdknssNFGVASMESDRKEP 292 thale cress
XP_006386388 212 LVAHNLAAPTLHNAGFAGSCSRIWQRDSYWNACPSSRSNEYPLFiprqnycsts--senswtdkssSNFGVASMESERRP 289
Q147F2       213 LVVHKLSVPALHNLGFAGSCSRIWERDSYWNSYPSSPSNGYPLFvpihdhysfeeksswtgrdttnFGVTSLKSDGSIEE 292 thale cress
Q9VAP6       346 LLSFYLNVRLPHKIAYGDFCR----------------------------------------------------------- 366 fruit fly
Q6ZNE5       316 ILSHILDVNLPKKLCNSEFCG----------------------------------------------------------- 336 human
EDO36555     287 LLASLLDVNLPSKLSYSEFCQ----------------------------------------------------------- 307
Q54QH5       219 LISGILGITLPYHLLSKGSKSTI--------------------------------------------------------- 241 Dictyostelium d...
Q4WCL3       314 SNHYQPRPLTIDRSLPKLAR--EDPESYVLFLEGVTLLAWNVSWLCRTQGLNI-------ASDSWEEICNIGKNMWQLLV 384 Aspergillus fum...
XP_002496378 239 ----------ELELEVSAKTadESSSDENTIVDHLTKIFINIIQISRHLNLISkd---piDLAWMLDQNDLDTLFYNMAM 305
Q6FX35       239 --------------LSLQGHsaKLKRNAPELLQVLIKLIQMILVVMTRSKLIDerk-qniDYAWILDQYDIDGLFYHMAM 303 [Candida] glabr...
Q8H1E1       293 RLDSPGSNSFKYSSASPHS-----IESHRDLQKGIALLKKSVACLTAY-CYNSlclevppEASTFEAFAKLLATLSSSKE 366 thale cress
XP_006386388 290 HLDSTRSNSFNYSSVSPHS-----VETHKDLQKGVSLLKKSVACVTAY-CYNLlcldvpsDTSTFEAFAKLLSTLSSSKE 363
Q147F2       293 DYHDLDLDVVSLSSASPHS-----VETFRNLQQGIAHLKQSVAHLTVY-GYRSlslevpsGTSTFETFAKLLATLSSLKE 366 thale cress
Q9VAP6       367 --------K---------------LLNEEQFRRKISRLNSNIMYLAYTQQVKLral---ieqHTLENL---LAILDLERS 417 fruit fly
Q6ZNE5       337 --------E---------------NLSKQKFTRAVKKLNANILYLCFSQHVNLdql---qplHTLRNL---MYLVSPSSE 387 human
EDO36555     308 --------N---------------EMGRKEFQSALSRLNTNVLHLCFTQHVDPtll---hphRTLNNL---YALINTTCT 358
Q54QH5       242 ------HHNMKSKDYPLYYQ---QKTKSREFQKALHLLGENIIFLRYYHGIQTp------KESNLLFL----KNIYELIK 302 Dictyostelium d...
Q4WCL3       385 A 385 Aspergillus fumigatus
XP_002496378 306 R 306
Q6FX35       304 S 304 [Candida] glabrata CBS 138
Q8H1E1       367 V 367 thale cress
XP_006386388 364 V 364
Q147F2       367 V 367 thale cress
Q9VAP6       418 D 418 fruit fly
Q6ZNE5       388 H 388 human
EDO36555     359 G 359
Q54QH5       303 S 303 Dictyostelium discoideum AX4
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