1MRU,2H34,3F61,4EQM


Conserved Protein Domain Family
STKc_PknB_like

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cd14014: STKc_PknB_like 
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Catalytic domain of bacterial Serine/Threonine kinases, PknB and similar proteins
STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. This subfamily includes many bacterial eukaryotic-type STKs including Staphylococcus aureus PknB (also called PrkC or Stk1), Bacillus subtilis PrkC, and Mycobacterium tuberculosis Pkn proteins (PknB, PknD, PknE, PknF, PknL, and PknH), among others. S. aureus PknB is the only eukaryotic-type STK present in this species, although many microorganisms encode for several such proteins. It is important for the survival and pathogenesis of S. aureus as it is involved in the regulation of purine and pyrimidine biosynthesis, cell wall metabolism, autolysis, virulence, and antibiotic resistance. M. tuberculosis PknB is essential for growth and it acts on diverse substrates including proteins involved in peptidoglycan synthesis, cell division, transcription, stress responses, and metabolic regulation. B. subtilis PrkC is located at the inner membrane of endospores and functions to trigger spore germination. Bacterial STKs in this subfamily show varied domain architectures. The well-characterized members such as S. aureus and M. tuberculosis PknB, and B. subtilis PrkC, contain an N-terminal cytosolic kinase domain, a transmembrane (TM) segment, and mutliple C-terminal extracellular PASTA domains. The PknB subfamily is part of a larger superfamily that includes the catalytic domains of other protein STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase.
Statistics
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PSSM-Id: 270916
Aligned: 166 rows
Threshold Bit Score: 143.111
Created: 5-Jul-2006
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 21 residues -Click on image for an interactive view with Cn3D
Feature 1:ATP binding site [chemical binding site]
Evidence:
  • Structure:3F61: Mycobacterium tuberculosis PknB mutant binds Mg2ADP; contacts at 4A.
  • Structure:4EQM: Staphylococcus aureus PknB binds ANP; contacts at 4A.
  • Structure:1MRU: Mycobacterium tuberculosis PknB binds gamma-thio-ATP; contacts at 4A.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1            ## #### #                       # #                                      
1MRU_B     13 RYELGEiLGFGGMSEVHLARDlr-----------lHRDVAVKVLRadla------rdpsFYLRFRREAQNAAALn----- 70  Mycobacterium tube...
3F61_A     13 RYELGEiLGFGGMSEVHLARDlr-----------dHRDVAVKVLRadla------rdpsFYLRFRREAQNAAALn----- 70  Mycobacterium tube...
EAS01023   52 SYVQFKyIGTGKQSLVLKVND--------------QFVVKIKKINrhn---------ekEIKDLDILVNAVRSLg----- 103 Tetrahymena thermo...
EAR89508   35 GYNIERqIGEGNFGVVYEASVqqdsiknqqliekdLKKVAVKLMKien---------eyTKIQVLNEIEIQKKIq----- 100 Tetrahymena thermo...
AAK43281  335 NYNIVDiIGVGGTSYILKGEKd-------------GNFYALKIPLiny---------lnNVMDLVGESSKLIELsn---- 388 Sulfolobus solfata...
AAL63049  106 RYKVIRlLGVGGFSYVLLVRQk-------------GKVYAAKILRyaddygvplasdenILRLFGYEMNRYLEIk----- 167 Pyrobaculum aeroph...
BAB67090  294 GYLVKEvIGEGGNGYVLKATGs------------dGKDYAIKVLKigtgs-----gsdeYFKNLVNEAYPLVSIs----- 351 Sulfolobus tokodai...
ABB15387  215 RYRLEDlIGEGNFSKVYWAYDnk-----------lDREVAVKEINltdl-------pgdIKDKIYNEAQLMAKIn----- 271 Carboxydothermus h...
AAF30624   14 KYKVVKhLADGGFSKVYLCCFls----------deTKFIVIKVLDind---------ekQQMVVYDELRISNLIknsdsd 74  Ureaplasma parvum ...
ABG50405   15 EIELLElLGTGTFGSAWKAIDhs-----------hKKNYILKVIQgi-----------kPEETLVERIRLEAAVni---- 68  Trichodesmium eryt...
Feature 1          #                               ####                         #             
1MRU_B     71 -HPAIVAVYDTGEaetp------------agplPYIVMEYVd----------------------GVTLRDIVHte----g 111 Mycobacterium tube...
3F61_A     71 -HPAIVAVYDTGEaetp------------agplPYIVMEYVd----------------------GVTLRDIVHte----g 111 Mycobacterium tube...
EAS01023  104 kENYIHQVDDSYFlqsdfq---------eskidLFYIQEMEfangklfqfnsfnkclfkntkgnFEDLFLQLRtks---i 171 Tetrahymena thermo...
EAR89508  101 lKGYALQIYQVVQtt----------------nvIAIVMELA-----------------------QCSLKQFLNe------ 135 Tetrahymena thermo...
AAK43281  389 kSPYIVRLYAIYAdqldvkeilggnpeiyynkpPMLVIELMk----------------------GGSINDVINvkelvks 446 Sulfolobus solfata...
AAL63049  168 -SDYIVKAFEVYLpavgyrdi-----kqymknpPYILLEYMe----------------------GGTLRDLLRar----k 215 Pyrobaculum aeroph...
BAB67090  352 nNPNVVRIYAIYAdeliikniisgntslylqdpPMIVMELMn----------------------GGTLLDLLSddkfyys 409 Sulfolobus tokodai...
ABB15387  272 -HENIVKLYDTFYfd----------------dkIYLIIELVq----------------------GKTLVEYLEekg---g 309 Carboxydothermus h...
AAF30624   75 kKNYIMEYYEYFEtgsle----------nndkrIYIVFEYIe----------------------GLTLREYLDef----k 118 Ureaplasma parvum ...
ABG50405   69 pSENIIPLLGLKEwda---------------ntYLILFEYFp----------------------GKRLDKLLEkk----- 106 Trichodesmium eryt...
Feature 1                                      # ## #                         ##              
1MRU_B    112 pMTPKRAIEVIADACQALNFSHq--NGIIHRDVKPANIMISa----------------tNAVKVMDFGIARAIadsgn-- 171 Mycobacterium tube...
3F61_A    112 pMTPKRAIEVIADACQALNFSHq--NGIIHRDVKPANIMISa----------------tNAVKVMDFGIARAIadsgn-- 171 Mycobacterium tube...
EAS01023  172 eLKDWQKERMAIQLFDALNIFHq--CGSCHRNIKPSNILYFyen-------------gnFTVKLADLQANNTEdfsqefn 236 Tetrahymena thermo...
EAR89508  136 kQYIQVIIDLMLQLLEGIKTLEe--NRIIHFDIRPDNILLDc----------------nLKAKFSDFNLSTLLdvdk--- 194 Tetrahymena thermo...
AAK43281  447 eYWKKIVFITTARIAEALETIHs--EGYVHCDVKPQNVLFNeklppnarlaydnlkngkIIVKLADLGSAVKAg------ 518 Sulfolobus solfata...
AAL63049  216 rLPVQQVIELFKQLAQGLYDIHk--HNIVHLDIKPENIMFTrd---------------rKIAKIGDMGIAKVVtgg---- 274 Pyrobaculum aeroph...
BAB67090  410 iYWRKTVYRAIASVAEALVEIHs--NGFVHCDIKPQNVFLTfkpkdp-------adlpnINFKLGDLGGAVKVg------ 474 Sulfolobus tokodai...
ABB15387  310 mLSVKKAVNIMIQIAKALSYIHq--HNIIHRDLKPENILISt----------------dDIVKITDFGIAQLNeee---- 367 Carboxydothermus h...
AAF30624  119 tITYVKAVEIIRQVALGVSFFHscnPQIIHRDLKPENCMINkt---------------lTKIKIIDYGAASVFynred-- 181 Ureaplasma parvum ...
ABG50405  107 sLNNRQKKQIFKQILLGISAAHq--CNIIHRDLKPANIIISn----------------dMQTKIIDFGVAKFKdqni--- 165 Trichodesmium eryt...
Feature 1                                                                                     
1MRU_B    172 -svtqtAAVIGTAQYLSPEQARg---------------------dSVDARSDVYSLGCVLYEVLTGeppftgdspvsvay 229 Mycobacterium tube...
3F61_A    172 -svtqtAAVIGTAQYLSPEQARg---------------------dSVDARSDVYSLGCVLYEVLTGeppftgdspdsvay 229 Mycobacterium tube...
EAS01023  237 qitnpvSSYKYIDVYQPLEAYLn----------------------QYHKKSDLFRLGLVLAQLDTPsfqftnisfekylq 294 Tetrahymena thermo...
EAR89508  195 --syisQCNGGTFKYISPESLLsdr-----------------nvhKITNKTDIYSLGIVFLELLGIqisfdeasdyrrnr 255 Tetrahymena thermo...
AAK43281  519 -----eKPFSYTPAYVSFDLVKsta------------------fgGVSPMADIYALGATVYKLLTGvtlntnvmieamdk 575 Sulfolobus solfata...
AAL63049  275 ----yvHSSYMSPAYAAPEVKKg----------------------LATFSSDIYSLGCVIYEALTGinpnvfvengyq-- 326 Pyrobaculum aeroph...
BAB67090  475 -----sSVVQLTTEYAPLEAFTd----------------------KAKPSFDVFSLGITLYVLLTGviyrpdsremedaf 527 Sulfolobus tokodai...
ABB15387  368 ---keeGFILGTPVVMAPEQILk---------------------tGIDQRTDIFALGCIFYYLLTGhypfsgtnfselsy 423 Carboxydothermus h...
AAF30624  182 --ltkdQEIKCTIIYASPKLLSlgqkvkeqankglnkralslindALGVSYDIHSLGVMLYELITGtnpfsehtitddrd 259 Ureaplasma parvum ...
ABG50405  166 ---tmsKEIIDTPPYMAPEFLIeg-------------------akVADAKSDIYSLGHILYELAMGenfwrhsgwykled 223 Trichodesmium eryt...
Feature 1                                                                     
1MRU_B    230 qh------------vredpippsarheglsADLDAVVLKALAKNPENRYQt---aAEMRADLVR 278 Mycobacterium tuberculosis
3F61_A    230 qh------------vredpippsarheglsADLDAVVLKALAKNPENRYQt---aAEMRADLVR 278 Mycobacterium tuberculosis
EAS01023  295 fqa-----------nfsenifpisdnikkdTFLYKIIQRCLHKNPSQRQQs----QYYLQSLIE 343 Tetrahymena thermophila SB210
EAR89508  256 q--------------kkldlsffnkdilryQLYDLIINNMINSNPDQRNI-----NQVYEFIIK 300 Tetrahymena thermophila SB210
AAK43281  576 feankdi--ryldnslystrnldllrkyvdKNTYLFISKMVDPDPNKRPTs----KEIKEFFYF 633 Sulfolobus solfataricus P2
AAL63049  327 ------------------ipppsayvadvpRWIDEIILKMLDLNPNARPNs----AELVNYLTR 368 Pyrobaculum aerophilum str. IM2
BAB67090  528 ncykvndmncvtrnvtiakqklalwdpnvpAEVKPLLKRMIDPDPLKRPTa----KEVLDQITR 587 Sulfolobus tokodaii str. 7
ABB15387  424 ni------------lhfdpepirtynshcsLEIEKIILKCLEKNPENRYQk---vSELLQDLIK 472 Carboxydothermus hydrogenoformans ...
AAF30624  260 ylek---------witydveplslinktipKGIDNILMRCFAWKKEDNKLlykdiYTLIDDLSN 314 Ureaplasma parvum serovar 3 str. A...
ABG50405  224 fikylt-----qtptpkigidlndfycdffPEVEQVLSQMMKINPEERYQs---vEDVICDLEI 279 Trichodesmium erythraeum IMS101

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