3UON


Conserved Protein Domain Family
7tmA_mAChR_M2

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cd15297: 7tmA_mAChR_M2 
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muscarinic acetylcholine receptor subtype M2, member of the class A family of seven-transmembrane G protein-coupled receptors
Muscarinic acetylcholine receptors (mAChRs) regulate the activity of many fundamental central and peripheral functions. The mAChR family consists of 5 subtypes M1-M5, which can be further divided into two major groups according to their G-protein coupling preference. The M1, M3 and M5 receptors selectively interact with G proteins of the G(q/11) family, whereas the M2 and M4 receptors preferentially link to the G(i/o) types of G proteins. Activation of M2 receptor causes a decrease in cAMP production, generally leading to inhibitory-type effects. This causes an outward current of potassium in the heart, resulting in a decreased heart rate. All GPCRs have a common structural architecture comprising of seven-transmembrane (TM) alpha-helices interconnected by three extracellular and three intracellular loops. A general feature of GPCR signaling is agonist-induced conformational changes in the receptors, leading to activation of the heterotrimeric G proteins, which consist of the guanine nucleotide-binding G-alpha subunit and the dimeric G-beta-gamma subunits. The activated G proteins then bind to and activate numerous downstream effector proteins, which generate second messengers that mediate a broad range of cellular and physiological processes.
Statistics
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PSSM-Id: 320424
Aligned: 7 rows
Threshold Bit Score: 454.424
Created: 4-Jul-2013
Updated: 25-Oct-2021
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 17 residues -Click on image for an interactive view with Cn3D
Feature 1:ligand binding site [chemical binding site]
Evidence:
  • Structure:3UON: Human M2 muscarinic acetylcholine bound an antagonist (3-quinuclidinyl-benzilate), contact at 4A.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                       #
3UON_A        24 VFIVLVAGSLSLVTIIGNILVMVSIKVNRHLQTVNNYFLFSLACADLIIGVFSMNLYTLYTViGYWPLGPVVCDLWLALD 103
P08172        24 VFIVLVAGSLSLVTIIGNILVMVSIKVNRHLQTVNNYFLFSLACADLIIGVFSMNLYTLYTViGYWPLGPVVCDLWLALD 103 Homo sapiens
P30372        27 VFIVLVAGSLSLVTIIGNILVMVSIKVNRHLQTVNNYFLFSLACADLIIGIFSMNLYTLYTViGYWPLGPVVCDLWLALD 106 chicken
XP_683133     32 VLIVLGLSILSLITIIGNVLVMLSIKVNRNLQTVNNYFLFSLACADLFIGVFSMNLYTVYIVtGHWPLGALVCDLWLALD 111 zebrafish
XP_002942846  27 VFIVIVAGSLSLVTIIGNILVMVSIKVNRHLQTVNNYFLFSLACADLIIGVFSMNLYTLYTViGYWPIGPVVCDLWLALD 106 western clawed ...
AAI63917      32 VFIVLVAGSLSLVTVIGNILVMLSIKVNRSLQTVNNYFLFSLACADLIIGLCSMNLYTVYIViGYWPLGPVVCDLWLALD 111 zebrafish
CAG03403       7 VLIVAVAGSLSLITVIGNILVMLSIKVNRNLQTVNNYFLFSLACADLIIGLCSMNLYTVYIViGHWPLGAVVCDLWLAVD 86  spotted green p...
Feature 1        #  ##                                              #                         #  
3UON_A       104 YVVSNASVMNLLIISFDRYFCVTKPltYPVKRtTKMAGMMIAAAWVLSFILWAPAILFWQFIVGVRTVedGECYIQFFsN 183
P08172       104 YVVSNASVMNLLIISFDRYFCVTKPltYPVKRtTKMAGMMIAAAWVLSFILWAPAILFWQFIVGVRTVedGECYIQFFsN 183 Homo sapiens
P30372       107 YVVSNASVMNLLIISFDRYFCVTKPltYPVKRtTKMAGMMIAAAWVLSFILWAPAILFWQFIVGGRTVpdKDCYIQFFsN 186 chicken
XP_683133    112 YVVSNASVMNLLIISFDRYFCVTKPlsYPVKRtHKMAGMMIAAAWILSFILWAPAILFWQFITGSRTVpeGECYIQFFsN 191 zebrafish
XP_002942846 107 YVVSNASVMNLLIISFDRYFCVTKPltYPVRRtTKMAGMMIAAAWVLSFILWAPAILFWQFIVGGRTVpeGECYIQFFsN 186 western clawed ...
AAI63917     112 YVVSNASVMNLLIISFDRYFCVTKPlsYPVKRtTKMAGMMIAAAWVLSFILWAPAILFWQFIVGGRTVpeKECYIQFFsN 191 zebrafish
CAG03403      87 YVVSNASVMNLLIISFDRYFCVTKPlsYPVRRsTKMAGLMIAAAWVLSFVLWAPAILFWQFIVGGRTVprGKCYIQFFsN 166 spotted green p...
Feature 1           #  ##  ##                                                                    
3UON_A       184 AAVTFGTAIAAFYLPVIIMTVLYWHISRASksrinifemlrideglrlkiykdtegyytigighlltkspslnaakseld 263
P08172       184 AAVTFGTAIAAFYLPVIIMTVLYWHISRASksrikkdkkepvanqdpvspslvqgrivkpnnnnmpssddglehnkiqng 263 Homo sapiens
P30372       187 PAVTFGTAIAAFYLPVIIMTVLYWQISRASksrikkgkkeaaqnqdpvspslvqgkivkpnnnniptssdglehnkvqng 266 chicken
XP_683133    192 AVVTFGTAIAAFYLPVITMTILYWQISKASrsrvrrrdnrrvsrvsndggqaglgnernmdrkstqegeemvsrkqsasd 271 zebrafish
XP_002942846 187 AAVTFGTAIAAFYLPVIIMTVLYWQISRASksrikkgkkeaapnqdpispsnvqgkivkpnnnnvsanedgldhgkiqng 266 western clawed ...
AAI63917     192 AAVTFGTAIAAFYLPVIIMMVLYWQVSRASksrvkkdnrkpsggnldvassnqirensankptnnnltaeetdrgqtqlt 271 zebrafish
CAG03403     167 PVVTFGTAIAAFYLPVAIMVHLYWRISKASrsrvkrdsrktsgtslgdgpdhsqedrcesqsnprgdrphgtggkedaeq 246 spotted green p...
Feature 1                                                                                        
3UON_A       264 kaigrntngvitkdeaeklfnqdvdaavrgilrn--------------------aklkpvydsldavrraalinmvfqmg 323
P08172       264 kaprdpvtencvqgeekessndstsvsavasnm---------------------rddeitqdentvstslghskdenskq 322 Homo sapiens
P30372       267 kttgesvmencvqgeekdssndstsvsvvpsn------------------------tkedeaakdasqisasqdhlkven 322 chicken
XP_683133    272 tttgeerdsendsisgsvlassnqrdde-------------------------------avsistngdirarqnqsasla 320 zebrafish
XP_002942846 267 kvtsnsgiencvqaegeekeisndstsvsvvntk--------------------degaakdvpqasgsqanpktensklt 326 western clawed ...
AAI63917     272 ddtinqhdaklqngkapstasgeaeeagqancipaeekessndstsgsgavtnqkeeaappssaaandsqtstrhrakag 351 zebrafish
CAG03403     247 qqnggapcrnnsrgqgdst-------------------------------------------------rdsvhpftsedt 277 spotted green p...
Feature 1                                                                                     #  
3UON_A       324 etgvagftnslrmlqqkrwdeaavnlaksrwynqtpnrakrvittfrtgtwdaypppSREKKVTRTILAILLAFIITWAP 403
P08172       323 tcirigtktpksdsctptnttvevvgssgqngdekqnivarkivkmtkqpakkkpppSREKKVTRTILAILLAFIITWAP 402 Homo sapiens
P30372       323 skltcirivtksqkgdccaptnttveivgtngdekqnsvarkivkmtkqpakkkpppSREKKVTRTILAILLAFIITWTP 402 chicken
XP_683133    321 teswvrftcfktasqkdlqntykvnsesqgmtngkerlslvsqkiisphsqkrksssSREKKVTRTIMAILVAFATTWTP 400 zebrafish
XP_002942846 327 cirivtkspkgdcsnatgttveivpgangrngedkqnivarkivkmtkqppkkklppSREKKVTRTILAILLAFIITWTP 406 western clawed ...
AAI63917     352 gskltcikiitkspkgdcyapsnatveivpaverqnhvarkivkmtkqppkkkkapsSREKKVTRTIMAILVAFVATWTP 431 zebrafish
CAG03403     278 tvtvaspkgpdagtktapthppspghsaadrqslvtrtlfkvtkqnavakwrrkgvsSREKKVTRTIMAILVAFVVTWTP 357 spotted green p...
Feature 1        ##                     #  ##                     
3UON_A       404 YNVMVLINTFCapCIPNTVWTIGYWLCYINSTINPACYALCNatFKKTF 452
P08172       403 YNVMVLINTFCapCIPNTVWTIGYWLCYINSTINPACYALCNatFKKTF 451 Homo sapiens
P30372       403 YNVMVLINSFCasCIPGTVWTIGYWLCYINSTINPACYALCNatFKKTF 451 chicken
XP_683133    401 YNVMVLINTFCsdCIPNTVWIFGYWLCYINSTVNPACYALCNvtFKNTF 449 zebrafish
XP_002942846 407 YNVMVLINTFCavCVPNTVWTIGYWLCYINSTINPACYALCNttFKKTF 455 western clawed frog
AAI63917     432 YNVMVLINTFCssCIPNTVWTIGYWLCYINSTINPACYALCNitFKKTF 480 zebrafish
CAG03403     358 YNVMVLINTFCssCIPNSLWTIGYWLCYINSTINPACYALCNasFKNTF 406 spotted green pufferfish

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