1DKG,3A6M,4ANI


Conserved Protein Domain Family
GrpE

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cd00446: GrpE 
Click on image for an interactive view with Cn3D
nucleotide exchange factor GrpE
GrpE is the adenine nucleotide exchange factor of DnaK (Hsp70)-type ATPases. In bacteria, the DnaK-DnaJ-GrpE (KJE) chaperone system functions at the fulcrum of protein homeostasis. GrpE participates actively in response to heat shock by preventing aggregation of stress-denatured proteins; unfolded proteins initially bind to DnaJ, the J-domain ATPase-activating protein (Hsp40 family), whereupon DnaK hydrolyzes its bound ATP, resulting in a stable complex. The GrpE dimer binds to the ATPase domain of Hsp70 catalyzing the dissociation of ADP, which enables rebinding of ATP, one step in the Hsp70 reaction cycle in protein folding. In eukaryotes, only the mitochondrial Hsp70, not the cytosolic form, is GrpE dependent. Over-expression of Hsp70 molecular chaperones is important in suppressing toxicity of aberrantly folded proteins that occur in Alzheimer's disease (AD), Parkinson's disease (PD), amyotrophic lateral sclerosis, as well as several polyQ-diseases such as Huntington's disease and ataxias.
Statistics
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PSSM-Id: 271355
Aligned: 150 rows
Threshold Bit Score: 102.969
Created: 6-Mar-2002
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 37 residues -Click on image for an interactive view with Cn3D
Feature 1:dimer interface [polypeptide binding site]
Evidence:
  • Structure:1DKG; E.coli GrpE coiled-coil dimer; contacts at 4.0A
  • Citation:PMID 9103205

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1       #  ##  ## ##  ### #  ##  ##  ## ##  ### ##### ##                      ## ###  
1DKG_A     56 TRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSLDRALEvadkanp------dmsamveDIELTLKS 129 Escherichia coli
AAC65203   62 NVLQEQYLRKAADLENYRKRALRERQEAVEHAYAALLADIVAVLDDFDRAIEaadhasste--veassafreGVLMIRKQ 139 Treponema pallidum...
AAM72712   53 QKLREEVMRRAAEFENFRKQKEREAALSGTRMLENIVRELLPLIDDLKRLMShipaemqam---aeakpfieGVELIHKN 129 Chlorobium tepidum...
AAQ66776   56 AALNDTHLRLMAEYDNYRKRTLKEKSELIRNGGEKVLVDLLPVIDDFERALSnlgdmse-------paaikeGVELIYSK 128 Porphyromonas ging...
AAS11122   84 RDWQDQYLRKAADFENYRKRMIREKQEAIDYANSNLLLDLVQVLDDFDRAIDagktqgge----svnnafveGVVMIKNQ 159 Treponema denticol...
AAT43424   46 EDYKNLYMRQRSEMENYQRYIEKTINNIKANANADLIKTMLPVLDSLDAGILhd-----------------eKLKPIRSQ 108 Picrophilus torrid...
AAW39350   41 EEYLDNLKRARAEFVNYKRYIEQERNVQSDMARGNAFMLVLPVLDDLERALAsvpadia-------ghpfveGLDLIVRK 113 Dehalococcoides et...
YP_446452  67 EELNERLLRKAAELENVRRRMDREKKRRHVAGKETVLESMLEVLDDFERSLDaaqdldvsedpesayetlkgGVEMVYRK 146 Salinibacter ruber...
B3QTT2     64 TNYREQLLRTVADFENLKKQKEREVASVRKFADESLIKELLPVLDDIERVLVnaskflqas---peaqsyvdGVKLIQQN 140 Chloroherpeton tha...
A0B748     49 DERLEQLLRCRAELDNVIKRNSREREELARFASEAIIKKLLVFLDSLEQAAKhd-----------------eGAKALYDQ 111 Methanosaeta therm...
Feature 1     #     ##                                                          
1DKG_A    130 MLDVVRKF-GVEVIAETnvPLDPNvHQAIAMVEsdd-vapGNVLGIM-QKGYTLNGRTIRAAMVTV 192 Escherichia coli
AAC65203  140 LSSVLETKyGLEYYPVLgeRFDPNlHEALSMSPsas--vhEKIVGAElQKGYRVRNRILRHAKVMV 203 Treponema pallidum subsp. pallid...
AAM72712  130 FMSLLERK-GVKEIEAKgkMLDVNfHEAITQIDapg-aepDTIVEEY-QTGYTLGDRVIRHAKVIV 192 Chlorobium tepidum TLS
AAQ66776  129 FMDYLQKQ-GVKKIETAdlPFDADlCDAVAMIPapsaeqkGKVIDCV-KTGYTLNDKVIRHAHVVV 192 Porphyromonas gingivalis W83
AAS11122  160 MVSMLSSKyGLSYYPAKgeAFDPNlHEAVSMIQspd-vkeAVVGEEL-QKGYKLKERVIRHSKVMV 223 Treponema denticola ATCC 35405
AAT43424  109 LIKILSNY-GLKEIESRgkKFDPYlNEVVGIVKgd----dDIVVEEV-QKGYILNNEVLRTSKVIV 168 Picrophilus torridus DSM 9790
AAW39350  114 FQAILDNQ-GVKAIPAAgePFDSRlHEAVACEDgp----eGIILHEA-RRGYTVGDKVLRTSLVVV 173 Dehalococcoides ethenogenes 195
YP_446452 147 FQDQLQSL-GVEPIEAEgqPFDEQlHEAMMRQPsdd-vepGNVLQEV-QKGYTMGDRVLRHSRVVV 209 Salinibacter ruber DSM 13855
B3QTT2    141 MMKVFEAR-GLKRIEAVgtPFDVHlHEALSQMEkeg-aepDTVIQEF-APGYTLNDKVVRHSKVIV 203 Chloroherpeton thalassium
A0B748    112 LLDIMRSE-GLEPIDAVgkKFDPFvHEAMMQVEsqe-aedGIVVQEF-QKGYTLHSRVIRTSKVAV 174 Methanosaeta thermophila

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