6F0X


Conserved Protein Domain Family
p31comet
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pfam06581: p31comet 
Mad1 and Cdc20-bound-Mad2 binding
This family is involved in the cell-cycle surveillance mechanism called the spindle checkpoint. This mechanism monitors the proper bipolar attachment of sister chromatids to spindle microtubules and ensures the fidelity of chromosome segregation during mitosis. A key player in mitosis is Mad2, and Mad2 exhibits an unusual two-state behaviour. A Mad1-Mad2 core complex recruits cytosolic Mad2 to kinetochores through Mad2 dimerization and converts Mad2 to a conformer amenable to Cdc20 binding. p31comet inactivates the checkpoint by binding to Mad1- or Cdc20-bound Mad2 in such a way as to stop Mad2 activation and to promote the dissociation of the Mad2-Cdc20 complex.
Links
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Statistics
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PSSM-Id: 461953
Aligned: 4 rows
Threshold Bit Score: 493.331
Created: 22-Mar-2022
Updated: 17-Oct-2022
Structure
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Program:
Drawing:
Aligned Rows:
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PubMed ReferencesClick to see Conserved Features Help
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
6F0X_P        10 SSAAVPDLEWYEKSEETHASQIElLET--SSTQEPLNASEAFCPRDcMVPVVFPGPVSQEGCCQFTCELLKHIMYQRQQL 87  human
Q9DCX1        13 SPAAAPNLDWYEKPEETHAPEVD-LETviPPAQEPSNPAEPFCPRD-LVPVVFPGPVSQEDCCQFTCELLKHILYQRHQL 90  house mouse
XP_006100650  10 SLTAAPGLEWYEEAEDTHAPHIElLDT--TAAQELPSRSESFRPRHgLVPVVFPGPVSQDGCCQFTCEFLKHVMYQRLQL 87 
XP_016286572  11 ddaqLSELEGIEESEESCAPQREqLES--TSTHDPSSNPEHLCPGDsLVPVVFPGPVNSEGCCRFACELLKHIMYQRQQL 88 

6F0X_P        88 PLPYEQLKHFYRKpSPQAEEMLKKKPRATTEVSSRKCQQALAELESVLSHLEDFFARTLVPRVLILLGGNALSPKEFYEL 167 human
Q9DCX1        91 PLPYEQLKHFYRK-VPQAEDTARKKAWLATEARNRKCQQALAELESVLSHLRDFFARTLVPQVLILLGGNALSPKEFYEL 169 house mouse
XP_006100650  88 PLPYEQLKHFYRQsSPQAEDMVKKKPWAITEASSRKCQQTLEELESVLSHLESLFARTLVPRVLILLGGSVLNPKEFYEL 167
XP_016286572  89 PLPYDQLTVFYSKpSPQAEDMMRKKA----ERSYRKFQQALLELEGILSHLESLFARTLVPRVLILLGGSALCPKEFYEL 164

6F0X_P       168 DLSLLAPYSVDQSLSTAACLRRLFRAIFMADAFSELQAPPLMGTVVMAQGHRNCGEDWFRPKLNYRVPSRGHKLTVTLSC 247 human
Q9DCX1       170 DLSRLAPFGVDQGLNTAACLRRLFRAIFLADPFSELQTPPLMGTIVMVQGHRDCGEDWFQPKLNYRVPSRGHKLTVTLSC 249 house mouse
XP_006100650 168 DLSRLAPSNVDQRVSTAACLRLIFRAIFMADAFSELQAPPLMGTIVMAQGHRDCGEDWFRPKLNYRVPIRGHKLTVTLSC 247
XP_016286572 165 DLARLALGRMGQSLSTSACLRRLFRALFMADAFSELKAAPAMGTIVMAQGHRECGEDWFRPKLNYQVPKRGHKLTVTLSC 244

6F0X_P       248 GRPSIRTTAWEDYIWFQAPVTFKGFRE 274 human
Q9DCX1       250 GRPSVPAMASEDYIWFQAPVTLKGFHE 276 house mouse
XP_006100650 248 GRPSIPVTAWEDYIWFQAPVTLKGFHE 274
XP_016286572 245 GGPPIPDSAWDDYIWFQAPVTLKGFRE 271
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