5QIO,1TFF,4FJV


Conserved Protein Domain Family
OTUB2

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cd22764: OTUB2 
Click on image for an interactive view with Cn3D
Ubiquitin Thioesterase Otubain-2
Otubain-2 is also called ubiquitin thioesterase OTUB2, deubiquitinating enzyme OTUB2, OTU domain-containing ubiquitin aldehyde-binding protein 2, or ubiquitin-specific-processing protease OTUB2. It is a deubiquitylase (DUB)/ubiquitin thioesterase (EC 3.4.19.12) that mediates the deubiquitination of 'Lys-11'-,'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains. OTUB2 plays a role in DNA double-strand break (DSB) response (DDR); it enhances RNF8-mediated ubiquitination in an early phase of the DDR and promotes faster DSB repair but suppresses homologous recombination. It also functions as a cancer stemness and metastasis-promoting factor that deubiquitinates and activates the transcriptional regulators YAP/TAZ, which play important roles in development, physiology, and tumorigenesis and are negatively controlled by the Hippo pathway. OTUB2 belongs to the OTU family of cysteine proteases that use a conserved cysteine, histidine, and an aspartate, as the catalytic triad. It is classified as a family C65 cysteine protease by MEROPS.
Statistics
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PSSM-Id: 438601
Aligned: 12 rows
Threshold Bit Score: 351.297
Created: 21-Sep-2020
Updated: 17-Oct-2022
Structure
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Program:
Drawing:
Aligned Rows:
 
polypeptideactive site
Conserved site includes 18 residues -Click on image for an interactive view with Cn3D
Feature 1:polypeptide substrate binding site [polypeptide binding site]
Evidence:
  • Structure:4FJV: Human OTUB2 binds polyubiquitin-c; contacts at 4A

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                   #                                    
5QIO_A         3 LISEKCDILSILRDHpenriYRRKIEELSkRFTAIRKTKGDRNCFYRALGYSYLESLLGkSREIFKFKERVLQTPNDLLA 82  Homo sapiens
1TFF_A         8 LISEKCDILSILRDHpenriYRRKIEELSkRFTAIRKTKGDRNCFYRALGYSYLESLLGkSREIFKFKERVLQTPNDLLA 87  Homo sapiens
4FJV_A        11 LISEKCDILSILRDHpenriYRRKIEELSkRFTAIRKTKGDGNCFYRALGYSYLESLLGkSREIFKFKERVLQTPNDLLA 90  human
Q96DC9         8 LISEKCDILSILRDHpenriYRRKIEELSkRFTAIRKTKGDGNCFYRALGYSYLESLLGkSREIFKFKERVLQTPNDLLA 87  human
XP_006010245   6 LVSDKCDISVLVDGYpedgiYKRKLQDLQkKYISIRRTRGDGNCFYRALAFAHLESLIGnNVEIKKFKDVVIQSRNELVS 85  coelacanth
NP_001088469   5 FISEKYDLALLIEENskvpvYQRKLKDLQnQYHSVRKTRADGSCFYRGLCFAYLEFLLGkKQEILRFKHLVVQSKSEVLM 84  African clawed ...
XP_007902079  55 LFGNRPDVETNLLHIpnqdlCGFILKDLSkRYACFRRTLGDGNCFYRALSFAYLEALLGnSKGVQKLRKILMRSKGELLS 134 elephant shark
TMS20920       6 LVSCREDISSLFPEQt----PGAKYKDLSsQFSSVRQVRGDGNCFYRALCFAHLESVLHsARALQRFKETIIQTCKDFAT 81  large yellow cr...
XP_018414898  34 LVSEKCDIASFIEEHagepaHQSKLKDLRpRYCAIRKARADGSCFFRGLAFSYLESLLGdRQEIVSFRERVTESRNELLA 113 Nanorana parkeri
XP_015206186  25 LISHKLDISTLLQEQp----EDTKYQDISqRYASIRRIKGDGNCFYRALSFGHLESLLEnARGLQFFKETLQQSRSELLY 100 spotted gar
XP_030070559  10 LISEKCDISVILDERpdeeiFQWKVKDLSkRYSSIRKTLGDGNCFYRALGYAYLESLLGnPREIHRFKEIVIRSKSELLS 89  Microcaecilia u...
XP_023658148   5 YIFEQQEISTALPDNp----EDRKYQELYtQYALIRRIRGDGNCFYRAVAFGCLESFRN-TEGMQMFRDTVLRSSKVLTI 79  Paramormyrops k...
Feature 1                                                                     #  #### #          
5QIO_A        83 AGFeEHKFRNFFNAFYSVVELVEKDgSVSSLLKVFNDQSASDhIVQFLRLLTSAFIRNRADFFRHFIDeEMDIKDFCTHE 162 Homo sapiens
1TFF_A        88 AGFeEHKFRNFFNAFYSVVELVEKDgSVSSLLKVFNDQSASDhIVQFLRLLTSAFIRNRADFFRHFIDeEXDIKDFCTHE 167 Homo sapiens
4FJV_A        91 AGFeEHKFRNFFNAFYSVVELVEKDgSVSSLLKVFNDQSASDhIVQFLRLLTSAFIRNRADFFRHFIDeEMDIKDFCTHE 170 human
Q96DC9        88 AGFeEHKFRNFFNAFYSVVELVEKDgSVSSLLKVFNDQSASDhIVQFLRLLTSAFIRNRADFFRHFIDeEMDIKDFCTHE 167 human
XP_006010245  86 AGFeDRAFQNTFIRFQGVIDLVEAEnSIVSLLSAFNDQDCSDhIVQYLRLLTSAFLQNRKDFFHHFIEgGMNIKDFCAQE 165 coelacanth
NP_001088469  85 AGFqEHLFKHHYETFLSIVELAELDgSISSLLKAFNQPCSSDsVVLYVRLVTSAYLRNRTEFYQHFIReGMTVVDFCAKN 164 African clawed ...
XP_007902079 135 MGFiESTFENAFNTFMDVIDLVEADvSGAKLLAVFNDRNTSDcLVLYLRLLTSAYLQKKSEFFEYFLEgGMSIKDFCTQE 214 elephant shark
TMS20920      82 AGFdESSFKHHLNTLVNVVEQCQADeQEDRLLQLFNEQATSDsVVQYLRLLTSAHLQNQADFFCHFVE-APNLLVYCHQE 160 large yellow cr...
XP_018414898 114 GGFqEQAFGRCYSTFLSVVDLAESDgSVSALLRAFNNPDISDnAVRYLRLVTSAFLRNRAEFYQPFVEeGLQVTDFCTQY 193 Nanorana parkeri
XP_015206186 101 AGFdENRFTDLLNTFVCVIEQTQEGgQGSTLLNLFNDQTTSDaIVHYLRLLTSAYIQNHADFFQHFVE-APNLKDYCNQE 179 spotted gar
XP_030070559  90 AGFdENVFRNYFNDFFSVIELIERNgSSTSLLRAFNDQNWSDcIVQYLRLLTSAFLRNRADFFQHFVDdGMAIKDFCAQE 169 Microcaecilia u...
XP_023658148  80 SGIsEDRFRALFSTFVNVVERSEADaHGNTLLEMFNEQTTSDsVVQYLRFLTSAHLQNHSERFQHFVE-APNLKAYCTQE 158 Paramormyrops k...
Feature 1              ####                 #        # #   #         #  # #    
5QIO_A       163 VEPMATECDHIQITALSQALsIALQVEYVDEMDtaLNHHVFPEAAtPSVYLLYKTSHYNILY 224 Homo sapiens
1TFF_A       168 VEPXATECDHIQITALSQALsIALQVEYVDEMDtaLNHHVFPEAAtPSVYLLYKTSHYNILY 229 Homo sapiens
4FJV_A       171 VEPMATECDHIQITALSQALsIALQVEYVDEMDtaLNHHVFPEAAtPSVYLLYKTSHYNILY 232 human
Q96DC9       168 VEPMATECDHIQITALSQALsIALQVEYVDEMDtaLNHHVFPEAAtPSVYLLYKTSHYNILY 229 human
XP_006010245 166 VEPMAMECDHIQIVAISQALeVSVQVEYVDEADteVNNHIFPEGArPSVYLLYKTAHYDILY 227 coelacanth
NP_001088469 165 VEQMATECDHIQIIALTQALeIPLQVEYVDKTEnvINHHIFPEGRsPSVFMLFLEHHFNILY 226 African clawed frog
XP_007902079 215 VEPMAMESDHIHITALTQALnIPIQVEYMDKSNtaVNHHIFPEGSkPLVYLLYKPGHYDILY 276 elephant shark
TMS20920     161 VEAMAMECDHVDILALSQALgICIHIVSMEGDDqqLAHHVIPEGAePSLHLLYQTSHYNILY 222 large yellow croaker
XP_018414898 194 VEPMTAVCDHIHISALTQALaIPLQVEYVDSMDsaINQHTFPDGArPSIYMLYKQDHYTVLY 255 Nanorana parkeri
XP_015206186 180 VEAMAMECDHLEILTLSQALgVGIKIECMEGNSg-LNHHIIPEGTqPTLHFLYKTSHYDILY 240 spotted gar
XP_030070559 170 VEPMAMECDHIQITALSQALeIPLQVEYVDETStaVNYHRFPETAiPSVHMLYTRAHYNILY 231 Microcaecilia unicolor
XP_023658148 159 VETMAMECDHVEILALSEALgVTIRIVSVEGGSgsLVFHTIPEEVeATLHLLYKNSHYDLLY 220 Paramormyrops kingsleyae

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