Conserved Protein Domain Family
GCS

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pfam03074: GCS 
Glutamate-cysteine ligase
This family represents the catalytic subunit of glutamate-cysteine ligase (E.C. 6.3.2.2), also known as gamma-glutamylcysteine synthetase (GCS). This enzyme catalyzes the rate limiting step in the biosynthesis of glutathione. The eukaryotic enzyme is a dimer of a heavy chain and a light chain with all the catalytic activity exhibited by the heavy chain (this family).
Statistics
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PSSM-Id: 460796
Aligned: 97 rows
Threshold Bit Score: 408.461
Created: 21-Mar-2022
Updated: 17-Oct-2022
Structure
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Aligned Rows:
Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
XP_002614386 243 VYMDSMGFGMGSSCLQVTMQAANMAQARYLYDALAPLTPVMLAVSAAAPIFKG-FLVDQDVRWNVISGAVDDRTFAERDE 321
XP_023819156 237 IYMDAMGFGMGNCCLQVTFQACSIDEARYLYDQLATICPIVMALSAASPFYRG-YVSDIDCRWGVISASVDDRTQEERGL 315
5_pfamImport   1 IYMDAMGFGMGNCCLQVLAQVC-VSALKSIFMKSNGCIPVILSSSSAGPGYSGiWVTSFTFFWPVLTRVKNLKKIKEKGA 79 
P97494       236 IYMDAMGFGMGNCCLQVTFQACSISEARYLYDQLATICPIVMALSAASPFYRG-YVSDIDCRWGVISASVDDRTREERGL 314 house mouse
EDO46274     238 IYLDAMGFGMGCSCLQMTFQACSIEEGRHLYDQLAAVTPIVMALSAGTPVFRG-YLGDLDCRWSVIAGSVDDRTPEERGL 316
XP_002588470 239 IYMDCMGFGMGCSCLQMTFQACNIDEARHLYDQLAPLCPIFLALSAAAPVYRG-YLADVDCRWNIIAGSVDCRTREERGL 317
XP_966349    232 VYMDAMGFGMGCCCLQLTFQACNITEARTLYDQLTPLCPIMLALTAASPLHRG-FVTDVDCRWNVISCSVDCRTKEERGL 310
XP_001862212 230 VHMDAMGFGMGCCCLQLTFQACNITEARTLYDQLTPMCPIMLALTAASPAYRG-YLTDVDCRWNVISASVDCRTREEKGE 308
XP_001947007 239 VYMDAMAFGMGCCCLQMTFQACCIKEARTLYDQLTPLCPILLALTAASPVFRG-YLTDVDCRWNVISGSVDCRTRQERGL 317
AHZ21071     233 VYMDAMGFGMGCCCLQLTFQACCITEARTLYDQLAPLCPIMLALSAASPIYRG-YLTDVDCRWNVISASVDCRTREERGL 311
XP_002614386 322 EPyagyeffggldvpeeVKShfqalgggrglngdavvnkfgdtgvktvdgrsvqK-IPKSRYDSIDSYLgdeayrreGFF 400
XP_023819156 316 QP---------------LKNn--------------------------------kFrIYKSRYDSIDSYL--------SAC 340
5_pfamImport  80 SCy--------------LRN----------------------------------NfHKSAQFPPVNLFS--------GTL 103
P97494       315 EP---------------LKNn--------------------------------rFrISKSRYDSIDSYL--------SKC 339 house mouse
EDO46274     317 KP---------------LKEn--------------------------------rFvIPKSRYDSISCYL--------SPE 341
XP_002588470 318 EP---------------LKDd--------------------------------kFvIPKSRYDSIDCYL--------TPE 342
XP_966349    311 AP---------------LKNd--------------------------------kFvIKKSRYDSIDSYL--------SPQ 335
XP_001862212 309 IP---------------LKNd--------------------------------rFrINKSRYDSIDSYL--------SPA 333
XP_001947007 318 EP---------------LTTd--------------------------------kFvIPKSRYDSTTLYL--------SSQ 342
AHZ21071     312 EP---------------LKNn--------------------------------kFrIQKSRYDSIDSYL--------SPQ 336
XP_002614386 401 RPAYNDINSPLNGAV--YERLVSEKFDEPLARHFAHLFVRDPLVIFNERVAQDNLSDNDHFENIQSTNWQTLRFKPPAly 478
XP_023819156 341 GEKYNDIELIIDEEI--FKQLLEAGIDKPLAQHIAHLFIRDPLSVFEEKLHLDDENESDHFENLQSTNWQTMRFKPPP-- 416
5_pfamImport 104 ATAYYALNWVLDKRLrqFQAVRQQ-VEGLVPGPVDHTSVYAPVAQILKI-KLSDLGSQS---GIQPSRWQR--------- 169
P97494       340 GEKYNDIDLTIDKEI--YEQLLEEGIDHLLAQHVAHLFIRDPLTLFEEKIHLDDANESDHFENIQSTNWQTMRFKPPP-- 415 house mouse
EDO46274     342 GTKYNDIELVMDQEI--YQQLVENGIDDALARHYAHLFIRDPMTLFKEHVDEDDEQYSDHFENIQSTNWQTMRFKPPP-- 417
XP_002588470 343 AQKYNDIDLVLDQDI--CQQLEDAGIDRLLARHFAHLFIRDPISLFSEKIHLNDDEESDHFENIQSTNWQSMRFKPPP-- 418
XP_966349    336 GEKFNDVPLIYDPKI--YQQLVDNDIDHLLAQHIAHLFIRDSVSLFSEKINQNDEEDTDHFENIQSTNWQTMRFKPPP-- 411
XP_001862212 334 GEKYNDVPLVMDEAL--YKRLREGDIDHLLAQHIAHLFIRDSVSLFSEKVHQNDKEDTDHFENIQSTNWQTMRFKPPP-- 409
XP_001947007 343 GEKFNDVPLVYDETI--YNKLKSADIDHQMAQHVAHLFIRDTVSLFNEKVHQDDTVEMDHFENIQSTNWQTMRFKPPP-- 418
AHZ21071     337 NEKYNDIPIVHDQNI--YKRLREGGIDHPLALHVAHLFIRDTVSLFSEKVHQDDENDTDHFENIQSTNWQTMRFKPPP-- 412
XP_002614386 479 kdatPEelARtPG-----WRVEFRPLEIQLTDFE--NAAFSVFIALAGAAILAYNPDWYIPISKIDANMKTAHKVDAATK 551
XP_023819156 417 ----PN--SE-IG-----WRVEFRPMEVQLTDFE--NAAYVVFVVLLTRVILSYKLDFLIPLSKVDENMKVAQERNAVLE 482
5_pfamImport 170 --------NEkLKhgkllWKVQC------LCNLElkNSLYQKFQVILT------KLDRLLGLK-VDENMKVAQKRDAVLQ 228
P97494       416 ----PN--SD-IG-----WRVEFRPMEVQLTDFE--NSAYVVFVVLLTRVILSYKLDFLIPLSKVDENMKVAQKRDAVLQ 481 house mouse
EDO46274     418 ----PN--SP-IG-----WRVEFRPTEVQLTDFE--NAAFVTFIVLLSRVILSFDLNLLMPISKVDANMQTGQMRDAVRQ 483
XP_002588470 419 ----PN--SP-IG-----WRVEFRPTEVQLTDFE--NAAYVVFIVLLTRVILSYNLNFLIPISKVDENMQEAQKRDAVRQ 484
XP_966349    412 ----PN--ST-IG-----WRVEFRPCEVQITDFE--NAAIVCFVVLLTRVILSYNLDFLIPISRVDENMQNAQKRNACME 477
XP_001862212 410 ----PN--SP-IG-----WRVEFRPCEAQLTDFE--NAAIVCFVVLLTRVILSYQLDFLIPISKVDENMQTSQKRGAVLT 475
XP_001947007 419 ----PK--ST-IG-----WRVEFRPCEVQLTDFE--NAAIVCFVVLLTRVILSYQLNFLIPISKVDENMSKAQKRSAALT 484
AHZ21071     413 ----PN--SS-IG-----WRVEFRPCEAQLTDFE--NAAYVCFVVLLTRVILSYQLNFVMPISKVDENMQRAQRRGACLN 478
XP_002614386 552 EKFWFRNpttcglrnddfkaydlswfdRFTTPGNDASAAYTNGyvtq-----devsSNVTDVKMTANEIVNGT---DSFP 623
XP_023819156 483 GMFYFRK--------------------DIFKGCHPVLDGNTSAqngv-----qtdgGSDEYTLMSIDTIINGKe--GVFQ 535
5_pfamImport 229 GMFYFRK--------------------DICKGGNAVVDGCGKAqqsae----laaeEHEEYALMSIDTIINGKe--GVFP 282
P97494       482 GMFYFRK--------------------DICKGGNAVVDGCSKAqss-------sepAAEEYTLMSIDTIINGKe--GVFP 532 house mouse
EDO46274     484 GKFYFRK--------------------TLKNCKPICAGKPCRPggfng----meciPCDELNQMSIDAIINGKndeGEFP 539
XP_002588470 485 GLFWFRK--------------------EVLPPNECCEEDPKPKpaaksysqyngscQQEEYTKMSINEIINGQa--GGFQ 542
XP_966349    478 QKFWFRK--------------------EVTTQISPPEANECCKkgttn----cepkNCDVYTCMTVNEIINGKd--GEFP 531
XP_001862212 476 EKFWFKK--------------------NINGAKENNHSNESNG-------------LDAEYELMTIDQIINGK---GCFP 519
XP_001947007 485 EKFWFRK--------------------NIAASTSKTANVNVNSs-----------nDDASYTQMSINEIINGKt--GEFP 531
AHZ21071     479 EKFWWRR--------------------DVGAPRDVAPPHSDA---------------TDEYHEMSINDIVNGKe--GVFP 521
XP_002614386 624 GLIRMIVRYVAA---ELapkakvSELVTLQSYLQLVSMRAAGKIPTTARFLRNFVLDHPAYQRDSKVSDAINYDLVAETV 700
XP_023819156 536 GLIPILNCYLENmevDV------DTRCTILNYLKLIKKRASGELMTMAKWMREFVAKHPHYKQDSVVTDKINYDLMKKCD 609
5_pfamImport 283 GLIPILNCYLENmevDV------DTRCSILNYLKLIKKRASGELMTVAKWMREFIANHPDYKQDSVITDQINYSLILKCN 356
P97494       533 GLIPILNSYLENmevDV------DTRCSILNYLKLIKKRASGELMTVARWMREFIANHPDYKQDSVITDEINYSLIWKCN 606 house mouse
EDO46274     540 GLIPLINKYLSDvdvDI------DTRCTISQYLKLISRRASGELMTTARWMREFIQSHPDYKHDSVVSERINYDLLNTCN 613
XP_002588470 543 GLVSLMNSYLETveiDV------DTRCSVQQYLRLIQKRASGELMTTARWIRAFVQNHPKYKYDSVVSDEIAYDLTKVSD 616
XP_966349    532 GLIPLINTYLSGmdvDA------DTHCTIQQYLKFIQRRASGEILTTASWLRKFVTEHPSYNKDSKVTEEINYDLLQTIK 605
XP_001862212 520 GLVPLINSYLGSmdvDA------DTHCTIQQYLKLIQKRASGELLTTASWIRKEITEHEEYKHDSVVSERICYDLLKKGK 593
XP_001947007 532 GLIPLIHSYLSSmevDT------DTHCTIQQYLKLISRRASGQIDTTASWIRRFVQDHPAYKRDSVVSDEINYDLLMTAD 605
AHZ21071     522 GLIPLIESYLSGmdvDA------DTHCSVQQYLKLIQRRAAGDIVTTASWLRAFVTNHPHYKKDSVVTEKINYDLLKTAH 595
XP_002614386 701 AI 702
XP_023819156 610 RI 611
5_pfamImport 357 QI 358
P97494       607 QI 608 house mouse
EDO46274     614 KI 615
XP_002588470 617 EI 618
XP_966349    606 GI 607
XP_001862212 594 DI 595
XP_001947007 606 GI 607
AHZ21071     596 GI 597
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