3BBE,3O7B,3BBD,3OII,5FAI,5JPQ,2V3K


Conserved Protein Domain Family
Nep1-like
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cd18088: Nep1-like 
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18S rRNA (pseudouridine(1248)-N1)-methyltransferase Nep1
18S rRNA (pseudouridine(1248)-N1)-methyltransferase Nep1 (also known as EMG1) methylates pseudouridine at position1248 (Psi1248) in 18S rRNA and is required for small subunit (SSU) ribosomal RNA (rRNA) maturation. Mutations on human cause in Bowen-Conradi Syndrome. Nep1 is a member of the SPOUT (SpoU-TrmD) methyltransferase (MTase) superfamily, a large class of S-adenosyl-L-methionine (AdoMet or SAM)-dependent RNA MTases which are structurally characterized by a deep trefoil knot.
Links
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Statistics
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PSSM-Id: 349961
Aligned: 84 rows
Threshold Bit Score: 164.24
Created: 1-Oct-2008
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
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SAM bindingdimer interface
Conserved site includes 17 residues -Click on image for an interactive view with Cn3D
Feature 1:SAM binding site [chemical binding site]
Evidence:
  • Structure:3BBD; Methanocaldococcus Nep1 with bound SAM, contacts 4A
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1                                                                                     
3BBE_A      3 YNIILAKSALELIpeeiknki-------rksrvykYDILdSNYHYKAMeklkd--------------------------- 48  Methanocaldococcus...
3BBD_A      3 YNIILAKSALELIpeeiknki-------rksrvykYDILdSNYHYKAXeklkd--------------------------- 48  Methanocaldococcus...
CDR96481    3 INFVLCNASLELHrershkr---------rslpqsFTLLnEEDHYREVatskpseqdadqnleeedvtedsaasegkfrr 73  Babesia bigemina
ETW41413  211 YKIVLLFSPLALTsikn---------------kqkTCIInADDHMNMFmnklenlenaikfekkek-ekrkleqiiqsvk 274 Plasmodium falcipa...
Q8ZW45      1 MILVLAESALELVpkeiwnhpavladarrrgkrpgEILLdRARHHPAMrllad--------------------------- 53  Pyrobaculum aeroph...
BAM41009    3 INFVICNASLELYfgskk------------ynqtkLTLLqEHIHNTRSlndvdflnddqdeepa-----qtnrkrpssar 65  Theileria oriental...
A4WMI3      1 MILVLAESALELVpreiwshpavvadarrrgkkpgGILLdRSRHHPAMavlld--------------------------- 53  Pyrobaculum arsena...
AFH43255   20 YIIGLVESSLEPLpkelvnskkavilsrrfniepkYLILdKSLFYEEMkvlke--------------------------- 72  Fervidicoccus font...
Q8TYU0      4 LCFVLAESELEFIppklrghqhvvrwakrrgkkpgECLLiASKHHVAMrdkrlp-------------------------- 57  Methanopyrus kandleri
ADN51196    4 VILLLTEASLETIpkellsdpviirdakrrgvnprYLILdRARHHRAMtrlpn--------------------------- 56  Vulcanisaeta distr...

Feature 1                                                                                     
3BBE_A     49 -kemrGRPDIIHISLLNILDSPInhekkLNIYIHTydDKVLKINPETRLPRnYFRFLGVMEKVLkge----------rnh 117 Methanocaldococcus...
3BBD_A     49 -kexrGRPDIIHISLLNILDSPInhekkLNIYIHTydDKVLKINPETRLPRnYFRFLGVXEKVLkge----------rnh 117 Methanocaldococcus...
CDR96481   74 rnwkdVRPDILHFCLLALHDSILnkegrMCIWVHTlnSQLYRFYPAFRIPRtFKVFNKVMAKYLhtnarclkpegddatv 153 Babesia bigemina
ETW41413  275 nklenIRLDILFFTLLSLRDSILnkkgkLQIYIYTvnGSFIFVSPLFRVPRnFTLFKKVMLNLLkrgvvy----ddqknv 350 Plasmodium falcipa...
Q8ZW45     54 -akrrGRPDIVHQVLLAFQYSLLakrglGKAYIHTrdDYVIAVSPEARVPKnYNNFVALIEQLFalgkvp-----pkgep 127 Pyrobaculum aeroph...
BAM41009   66 rdwseVRPDIVHFCLLALHDSILnkegrIQVYVQTldGKLFKVARDFRVPRtFKVFNKVFANYLhsksrsl--ttesgev 143 Theileria oriental...
A4WMI3     54 -srrrGRPDIVHQALLVFQYSLLaarglGRMYIHTlgDYVISVDPSTRVPKnYNNFVSLVEQLFatgrvp-----pegrp 127 Pyrobaculum arsena...
AFH43255   73 -kekrGRPDIIHQFLLATQYSYLnlkgkLKVFIHTinNDIIEVNPITRIPKnYFQFVSLIQNLYkykkvp-----rsgea 146 Fervidicoccus font...
Q8TYU0     58 erdrrGRPDIVHVTLLHVLDSPAsrenaLDVYVHTrhDRVIWFRGDVRLPRdQYRFIGLMEQVLkegqap----pdsdep 133 Methanopyrus kandleri
ADN51196   57 -aekrGRPDILHQALLLIQGSLLarnnlVKTYVHTvnDLVIDVDPEIRPPRnYDNFIGLMSQLFevgrvp-----pvgkp 130 Vulcanisaeta distr...

Feature 1                             ###                          ## ## #                    
3BBE_A    118 LIKMEEk-TLEDLlneinakKIAIMTKTG-KLTHpkl--------lkeYDTFIIGGFPYGKLKinkek------------ 175 Methanocaldococcus...
3BBD_A    118 LIKXEEk-TLEDLlneinakKIAIXTKTG-KLTHpkl--------lkeYDTFIIGGFPYGKLKinkek------------ 175 Methanocaldococcus...
CDR96481  154 LVERMDq-RLEDLge---ggVKVAVSSRG-RLQCaetffadtvkddlgNLWIYVSLSHYTPLPvpnesgilcvadlhkky 228 Babesia bigemina
ETW41413  351 LLKIIFn-DITSYve---daVCIAISNKG-FPTDvkkltdk-ikqtknNYFFFISLSNSHDVTkfmdiikk--------- 415 Plasmodium falcipa...
Q8ZW45    128 LMELYRk-DLATLlqe-lggVWAVFHESG-ARKPlsqmg-----sellKSVVVVGGFPHGDFQnkwv------------- 186 Pyrobaculum aeroph...
BAM41009  144 LVELLEq-PLDSLipe--gaRRVAVDNKC-PNSNlsalisd-lkpvidDSWFFVSVSPSRPLDsicpsgvvdvskvhkri 218 Theileria oriental...
A4WMI3    128 LMEIRRq-GLRDLlte-lggRWVVMHEAG-LRIPlvqlg-----kevlDSVVVIGGFPHGDFNnkwv------------- 186 Pyrobaculum arsena...
AFH43255  147 LLILRKnvKLSEYlkeknieKPILLHEKA-EKVEynele----kfyfpPCSFLIGGFPHGDFSkdtk------------- 208 Fervidicoccus font...
Q8TYU0    134 LIEVLDv-SVWDVlea--neVNVLLSERG-DLIEpvgymaglldagveRIGVVVGGFPKGDFSeefy------------- 196 Methanopyrus kandleri
ADN51196  131 LMRFVN--GINYVlntespdRKILLDDVKgKEVSlksfid--yvknfkRPLIMIGAFPRGSFEerty------------- 193 Vulcanisaeta distr...

Feature 1                                            #### # ## #  #         
3BBE_A    176 --------------------------------vfgdIKEISIYNK-GLMAWTVCGIICYSLS 204 Methanocaldococcus jannaschii
3BBD_A    176 --------------------------------vfgdIKEISIYNK-GLXAWTVCGIICYSLS 204 Methanocaldococcus jannaschii
CDR96481  229 ldranstpv-kptysqdledellteetttskpepsfDHNISISTT-YPTAMSTCYKLVCAIE 288 Babesia bigemina
ETW41413  416 ----------------------------ektktfsyDYLVRLSDL-QLSAVALTSKLTHFLN 448 Plasmodium falciparum NF135/5.C10
Q8ZW45    187 --------------------------------vekaAVVYSLGEE-SLDAAQVICKAVTAAE 215 Pyrobaculum aerophilum str. IM2
BAM41009  219 lsrdavkkspadanhlesleqsflnykrefkqsckyDHCIAIKEY-NLSCLANCFTLICSLE 279 Theileria orientalis strain Shintoku
A4WMI3    187 --------------------------------leeaAARYSLGEV-AMDAAQVACRVVAAAE 215 Pyrobaculum arsenaticum DSM 13514
AFH43255  209 ---------------------------------mvaWRSISLKKDfIMDAWMVADRIICHLE 237 Fervidicoccus fontis Kam940
Q8TYU0    197 ---------------------------------draDDVVRIYDE-PLDAWTVAARIVTAFE 224 Methanopyrus kandleri
ADN51196  194 ---------------------------------glaDDVLKIGKY-IMDTSSILCRLLTALE 221 Vulcanisaeta distributa DSM 14429

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