Conserved Protein Domain Family
TRX_CDSP32

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cd02985: TRX_CDSP32 
TRX family, chloroplastic drought-induced stress protein of 32 kD (CDSP32); CDSP32 is composed of two TRX domains, a C-terminal TRX domain which contains a redox active CXXC motif and an N-terminal TRX-like domain which contains an SXXS sequence instead of the redox active motif. CDSP32 is a stress-inducible TRX, i.e., it acts as a TRX by reducing protein disulfides and is induced by environmental and oxidative stress conditions. It plays a critical role in plastid defense against oxidative damage, a role related to its function as a physiological electron donor to BAS1, a plastidic 2-cys peroxiredoxin. Plants lacking CDSP32 exhibit decreased photosystem II photochemical efficiencies and chlorophyll retention compared to WT controls, as well as an increased proportion of BAS1 in its overoxidized monomeric form.
Statistics
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PSSM-Id: 239283
Aligned: 6 rows
Threshold Bit Score: 171.513
Created: 12-May-2005
Updated: 2-Oct-2020
Structure
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Aligned Rows:
 
catalytic
Feature 1:catalytic residues [active site]
Evidence:
  • Comment:based on known function of thioredoxin
  • Comment:Disulfide bond reduction by thioredoxin is initiated by deprotonation of the solvent-exposed N-terminal cysteine in the CXXC motif to form a thiolate. The pKa of this thiol must be near physiological pH to achieve maximal catalysis.
  • Comment:The C-terminal TRX domain of CDSP32 protein contains a redox active CXXC motif, which is replaced with SXXS in the N-terminal TRX-like domain.
  • Citation:PMID 9099998

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                               #  #                                                  
CAA71103  188 EDVEKVIQDHKid-kKLIVLDVGLKHCGPCVKVYPTVIKLSKQMaDTVVFARMNGDENDSCMQFLKDMDVIEVPTFLFIR 266 potato
NP_912164  78 HSAEELDGALRaakeRLVVVEFAASHSVNSSRIYPCMVELSRTC-GDVDFLLVMGDESDATRELCRREGITAVPHFTFYK 156 Japanese rice
NP_912164 193 ADVESLISDHRgeggKLVVLDVGLKRCGPCVKVYPTVVKLSRTMaDTTVFARMNGDENDSCMEFLRDMDVVEVPTFLFIR 272 Japanese rice
AAM63182   79 HSGEEFDEALKnaksRLVVAEFATSKSDQSNKIYPFMVELSRTC-NDVVFLLVMGDESDKTRELCRREKIEKVPHFSFYK 157 thale cress
AAM63182  194 PDVEKLIDENRtg-gKLIVLDVGLKHCGPCVKVYPTVLKLSRSMsETVVFARMNGDENDSCMEFLKDMNVIEVPTFLFIR 272 thale cress
CAA71103   73 NSMEELDEALKkaknRLVVVEFAGKDSERSKNIYPFMVNLSKTC-NDVDFLLVIGDETEKTKALCRREKIDKVPHFNFYK 151 potato
Feature 1                             
CAA71103  267 DGEICGRYVGSGKGELIGEILRYQ 290 potato
NP_912164 157 GAEKVHEEEGIGPDQLAGDVLYYG 180 Japanese rice
NP_912164 273 DGDIVGRYVGSGRGELIGEILRYN 296 Japanese rice
AAM63182  158 SMEKIHEEEGIEPDQLMGDVLYYG 181 thale cress
AAM63182  273 DGEIRGRYVGSGKGELIGEILRYS 296 thale cress
CAA71103  152 SMEKIHEEEGIGPDLLAGDVLYYG 175 potato

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