4YDU,3WUH,3ZEU,4WQ4,4WQ5


Conserved Protein Domain Family
ASKHA_NBD_TsaD-like
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cd24097: ASKHA_NBD_TsaD-like 
nucleotide-binding domain (NBD) of TsaD and similar proteins
TsaD (EC 2.3.1.234), also called N6-L-threonylcarbamoyladenine synthase, t(6)A synthase, t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaD, or tRNA threonylcarbamoyladenosine biosynthesis protein TsaD, is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. It is involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37, together with TsaE and TsaB. TsaD likely plays a direct catalytic role in this reaction. The family also includes mammalian OSGEP-like protein 1 (OSGEPL1) and yeast protein Qri7, which are the mitochondrial versions of the universal Kae1/TsaD (also known as YgjD) protein and essential for mitochondrial genome maintenance.
Links
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Statistics
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PSSM-Id: 466947
Aligned: 5 rows
Threshold Bit Score: 548.812
Created: 26-Jun-2022
Updated: 27-Apr-2023
Structure
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Program:
Drawing:
Aligned Rows:
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Conserved site includes 6 residues -Click on image for an interactive view with Cn3D
Feature 1:catalytic site [active site]
Evidence:
  • Comment:The catalytic residues of the YgjD/Kae1/Qri7 family whose mutations resulted in the abrogation of the biosynthesis of t6A
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
Feature 1             ##                                                                      
4YDU_A      3 VLGIETSCDETGIAIYDd-----eKGLLANQLYSQvklhadyggvvPELASRDHVRKTVPLIQAALKEsgltakDIDAVA 77  Escherichia coli
3WUH_B     11 VLAIETSCDDTCVSVLDrfsksaaPNVLANLKDTLdside--ggiiPTKAHIHHQARIGPLTERALIEsna-reGIDLIC 87  Saccharomyces cere...
3ZEU_B      3 VLGIETSCDETGIAIYDd-----kKGLLANQLYSQvklhadyggvvPELASRDHVRKTVPLIQAALKEagltasDIDAVA 77  Salmonella enteric...
4WQ4_A      3 VLGIETSCDATGIAIYDd-----eKGLLANQLYSQvklhadyggvvPELASRDHVRKTVPLIQAALKEsgltakDIDAVA 77  Escherichia coli K-12
4WQ5_A      3 VLGIETSCDETGIAIYDd-----eKGLLANQLYSQvklhadyggvvPELASRDHVRKTVPLIQAALKEsgltakDIDAVA 77  Escherichia coli K-12

Feature 1                                      #   #                                          
4YDU_A     78 YTAGPGlVGALLVGATVGRSLAFAWdVPAIPVHHMEGHLLApmled--nppeFPFVALLVSGGHTQLISVTgigQYELLG 155 Escherichia coli
3WUH_B     88 VTRGPGmPGSLSGGLDFAKGLAVAWnKPLIGVHHMLGHLLIprmgtngkvpqFPFVSLLVSGGHTTFVLSRaidDHEILC 167 Saccharomyces cere...
3ZEU_B     78 YTAGPGlVGALLVGATVGRSLAFAWnVPAIPVHHMEGHLLApmled--nppeFPFVALLVSGGHTQLISVTgigQYELLG 155 Salmonella enteric...
4WQ4_A     78 YTAGPGlVGALLVGATVGRSLAFAWdVPAIPVHHMEGHLLApmled--nppeFPFVALLVSGGHTQLISVTgigQYELLG 155 Escherichia coli K-12
4WQ5_A     78 YTAGPGlEGALLVGATVGRSLAFAWdVPAIPVHHMEGHLLApmled--nppeFPFVALLVSGGHTQLISVTgigQYELLG 155 Escherichia coli K-12

Feature 1                #                                                                    
4YDU_A    156 ESIddaAGEAFDKTAKLLgl-dyPGGPLLSKMAaqgtagrfv---------fprpmtdrPGLDFSFSGLKTFAANTIRDn 225 Escherichia coli
3WUH_B    168 DTIdiaVGDSLDKCGRELgfkgtMIAREMEKFInqdindqdfalklempsplknsaskrNMLSFSFSAFITALRTNLTKl 247 Saccharomyces cere...
3ZEU_B    156 ESIddaAGEAFDKTAKLLgl-dyPGGPMLSKMAsqgtagrfv---------fprpmtdrPGLDFSFSGLKTFAANTIRSn 225 Salmonella enteric...
4WQ4_A    156 ESIddaAGEAFDKTAKLLgl-dyPGGPLLSKMAaqgtagrfv---------fprpmtdrPGLDFSFSGLKTFAANTIRDn 225 Escherichia coli K-12
4WQ5_A    156 ESIddaAGEAFDKTAKLLgl-dyPGGPLLSKMAaqgtagrfv---------fprpmtdrPGLDFSFSGLKTFAANTIRDn 225 Escherichia coli K-12

Feature 1                                                                                     
4YDU_A    226 gtd------dqtRADIARAFEDAVVDTLMIKCKRALdqt-----gfKRLVMAGGVSANRTLRAKLAEMMkk----rrGEV 290 Escherichia coli
3WUH_B    248 gkteiqelpereIRSIAYQVQESVFDHIINKLKHVLksqpekfknvREFVCSGGVSSNQRLRTKLETELgtlnstsfFNF 327 Saccharomyces cere...
3ZEU_B    226 ggd------eqtRADIARAFEDAVVDTLMIKCKRALest-----gfKRLVMAGGVSANRTLRAKLAEMMqk----rrGEV 290 Salmonella enteric...
4WQ4_A    226 gtd------dqtRADIARAFEDAVVDTLMIKCKRALdqt-----gfKRLVMAGGVSANRTLRAKLAEMMkk----rrGEV 290 Escherichia coli K-12
4WQ5_A    226 gtd------dqtRADIARAFEDAVVDTLMIKCKRALdqt-----gfKRLVMAGGVSANRTLRAKLAEMMkk----rrGEV 290 Escherichia coli K-12

Feature 1              #               
4YDU_A    291 FYARPeFCTDNGAMIAYAGMVRFKA 315 Escherichia coli
3WUH_B    328 YYPPMdLCSDNSIMIGWAGIEIWES 352 Saccharomyces cerevisiae S288C
3ZEU_B    291 FYARPeFCTDNGAMIAYAGMVRFKA 315 Salmonella enterica subsp. enterica serovar Typhimurium str. ST4/74
4WQ4_A    291 FYARPeFCTDNGAMIAYAGMVRFKA 315 Escherichia coli K-12
4WQ5_A    291 FYARPeFCTDNGAMIAYAGMVRFKA 315 Escherichia coli K-12

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