2W5A,2WQM,4APC


Conserved Protein Domain Family
STKc_Nek

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cd08215: STKc_Nek 
Click on image for an interactive view with Cn3D
Catalytic domain of the Serine/Threonine Kinase, Never In Mitosis gene A (NIMA)-related kinase
STKs catalyze the transfer of the gamma-phosphoryl group from ATP to serine/threonine residues on protein substrates. The Nek family is composed of 11 different mammalian members (Nek1-11) with similarity to the catalytic domain of Aspergillus nidulans NIMA kinase, the founding member of the Nek family, which was identified in a screen for cell cycle mutants that were prevented from entering mitosis. Neks contain a conserved N-terminal catalytic domain and a more divergent C-terminal regulatory region of various sizes and structures. They are involved in the regulation of downstream processes following the activation of Cdc2, and many of their functions are cell cycle-related. They play critical roles in microtubule dynamics during ciliogenesis and mitosis. The Nek family is part of a larger superfamily that includes the catalytic domains of other STKs, protein tyrosine kinases, RIO kinases, aminoglycoside phosphotransferase, choline kinase, and phosphoinositide 3-kinase.
Statistics
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PSSM-Id: 270855
Aligned: 91 rows
Threshold Bit Score: 329.038
Created: 5-Jul-2006
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
Conserved site includes 15 residues -Click on image for an interactive view with Cn3D
Feature 1:ATP binding site [chemical binding site]
Evidence:
  • Structure:2W5A_A; Human Nek2 binds MgADP; defined at 4A contacts.

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1            ## ##   #            # #                              #                  
2W5A_A      7 DYEVLYtIGTGSYGRCQKIRRksdGKILVWKELDygsmteaekqMLVSEVNLLRELkHPNIVRYYDRIIdrtnttLYIVM 86  human
585578     19 KYLNKGiVGLGSYGEAYVAESvedGSLCVAKVMDlskmsqrdkrYAQSEIKCLANCnHPNIIRYIEDHEen--drLLIVM 96  Trypanosoma brucei...
AAX69250   40 RYVKKKlLGQGSFGSVWRVEEtatGSIFAAKVMDtnnmsakdrgFVINEVKCLSRCnNANIIRHHASFNrg--gmLLIIA 117 Trypanosoma brucei
CAJ06522   35 KYERVReLGSGSFGKALLVRRvsdGQLLVAKRMDlaamsekdkkYATGEIQCLASCdHFAIIKYYEDYVae--phILIIM 112 Leishmania major
CAJ04405   43 KYRVRRaIGRGAFSTVWLLINnktGKTVVGKLSDashsssackaFAEAEVENMRCCsHPNIISLIETFEag--ekSLYIL 120 Leishmania major
CAK88708    3 KYRRIRkIGKGNFGDVWLVEDn-kGRQYAMKRIDlqf----dtvDPQNEVNIMKVLkHPNIIKFYDSFEhn--dkLCIIM 75  Paramecium tetraur...
CAK77150    3 HFERIRkIGKGNFGDVLLVSRksdGKQFALKRVDlsmre-sfvvDPLNEVKVLKSLdHMNIIKHYDSFVhn--nkLCILM 79  Paramecium tetraur...
CAK75588  166 FYEVVRdLGRGAFGCVQLVRKstdQELYAMKVIPtsfmneqekkNAENEVSLLRVLtAPTIIKYYESFAen--dsLNIIM 243 Paramecium tetraur...
EAY18256    8 RYKRIQqIGKGSYGKVYLMKDnknGDYVVVKTIKikgsddssrkTAQKEATLLSNLrHPNIIAYIDSFYtpq-gdFSIVL 86  Trichomonas vagina...
EEZ98627    9 DYEKLKtVGQGAFGTATLYRRlndGKQVVMKEVFlmdmdnsekkSALNEVEILSNLnHPNIIKYLGSFQke--gsLVIIM 86  red flour beetle
Feature 1     ###   #                                                            # #          
2W5A_A     87 EYCEGGDLASVITkgtk--------erqYLDEEFVLRVMTQLTLALKECHrrsdgghtVLHRDLKPANVFLDgkQNVKLG 158 human
585578     97 EFADSGNLDEQIKlrgs-------gdarYFQEHEALFLFLQLCLALDYIHsh-----kMLHRDIKSANVLLTstGLVKLG 164 Trypanosoma brucei...
AAX69250  118 EYADGGDLCRQIKvrqh--------agrHFKEHEVLYIFLQLCLALDHIHgn-----nMMHRDLKSANVLLTtmGLVKLG 184 Trypanosoma brucei
CAJ06522  113 EFADAGDLNMQIKqrak-------agfiYFEEHEVGYTFVQLAMAVDHIHrr-----rMLHRDIKGANVMLMtnGVIKLG 180 Leishmania major
CAJ04405  121 EYANAGDLQAQVDtraqpppgtsdgtsiPYREDEVLVIFAQLGLAIRYLHdr-----rIMHRDLKTSNVLLTrsGLIKLG 195 Leishmania major
CAK88708   76 EYAKNCKSLDHIQadls--------iyiKTKQPDILNYFTQLCLGVQYLHqq-----qIVHRDIKLRNVFITddGIIKLG 142 Paramecium tetraur...
CAK77150   80 EYAENADLSLKIKeakh--------nnqFIQEPTILAWLTQLAVALNYLHsq-----kILHRDIKVQNIFLCndGIVKLG 146 Paramecium tetraur...
CAK75588  244 EYAEGGSLNEKISehir--------vgqKIPKDQILAWMAQLVIAIHFMHsk-----nILHRDIKTQNMFLNkeQVIKLG 310 Paramecium tetraur...
EAY18256   87 EYADGKDLQKYLEsh------------eEIKEKKVLQIFTQIILGLEYIHsq-----nILHRDIKTANVFLFkrGLVKLG 149 Trichomonas vagina...
EEZ98627   87 EYADGGNLAQLINakkh--------kneIFTEKSILNILSQISAAISYMHtn-----kILHRDLKSANIFLN--MNGNVK 151 red flour beetle
Feature 1          #                                                                          
2W5A_A    159 DFGLARILNhdt--sfaKTFVGTPYYMSPEQMNRMsYNEKSDIWSLGCLLYELcaLMPPFTAfsqKELAGKIREGk-FRR 235 human
585578    165 DFGFSHQYEdtvsgvvaSTFCGTPYYLAPELWNNKrYNKKADVWSLGVLLYEImgMKKPFSAsnlKGLMSKVLAGt-YAP 243 Trypanosoma brucei...
AAX69250  185 DFGFSRQYEdslsnpvgTTFCGTPYYLSPELWRRApYSKKSEMWALGVVLYEVmmLKRPFGGrnmDELINNIVHAr-RAP 263 Trypanosoma brucei
CAJ06522  181 DFGFSHQYEntvsnqvaQTFCGTPYYLAPELWNHQrYSKKADVWAMGILLFEMmaLRRPFVGqgmRALSEAVMSN--RRD 258 Leishmania major
CAJ04405  196 DFGFSRQYQesvsgevgKTFCGTPYYLAPEMWQRQsYSYKADIWSLGVIMYELlaLKKPFQAtnlSELMEMVTRQgsFDP 275 Leishmania major
CAK88708  143 DFSISKKLIdl----stNTTLGTPYYLSPEICQSKnYNSKTDIWNLGCLLYELctQQKPFQGdslPAILNTIINGq-TPQ 217 Paramecium tetraur...
CAK77150  147 DFGISRTLEnts--elaQTSIGTPFYLSPELCQNQsYNHKIDIWMLGCTIYELctLQKPFTAesiNALATKIINEq-HTK 223 Paramecium tetraur...
CAK75588  311 DFGISKALGthg--nfaQTFLGTPYFMPPEVIRGEpYGKKADIWALGCALYELvmLKRPFQHdvmQIVFDMIQNKp-YDM 387 Paramecium tetraur...
EAY18256  150 DFGISREVTeds---faQTMIGTPYFMCPELLRGDpYSFPADIWAAGCVLFELltHKHAFTGksrEELFTNIKSGn-MSM 225 Trichomonas vagina...
EEZ98627  152 VGDFGISKMlntr-sqaQTVVGTPYYLSPEMCEGSdYNEKSDIWAIGCILYELacLRKPFEAmtlPVLVQKITACe-YSN 229 red flour beetle
Feature 1                                           
2W5A_A    236 Ipy--rYSDELNEIITRMLNLkDYHRPSvEEILENPLI 271 human
585578    244 Lpd--sFSSEFKRVVDGILVAdPNDRPSvREIFQIPYI 279 Trypanosoma brucei brucei
AAX69250  264 Lpn--mYSDELRRVCDQLLSLdPAKRPSlRQLFQQPFI 299 Trypanosoma brucei
CAJ06522  259 CelpqqYSRSLKDLCNIMLQAdPNQRPScAQLFKLPHM 296 Leishmania major
CAJ04405  276 Lpe-drYSSDMISLVNQMLRVdPSERPSiKDILALPLF 312 Leishmania major
CAK88708  218 Lne--aFPKFYQDILNGTLQKnPDLRPDiEQLLNIPQI 253 Paramecium tetraurelia
CAK77150  224 Isd--hYSDFLSNLIDEMLQKqPEKRPEiSKILSFPQI 259 Paramecium tetraurelia
CAK75588  388 Dp---sVDSDLQQLIEKTLQKdPNKRPFvEDLANIPCI 422 Paramecium tetraurelia
EAY18256  226 Mps--gYSKELIELLMSMLQQdPNDRPTcKEILASNII 261 Trichomonas vaginalis G3
EEZ98627  230 Ipe--iYSDELSQLVHSILQRdPVARPSaKYIHDTIVP 265 red flour beetle

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