4JD2,1K8K,2P9K,2P9I,6DEC


Conserved Protein Domain Family
ASKHA_NBD_Arp2

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cd10220: ASKHA_NBD_Arp2 
nucleotide-binding domain (NBD) of actin-related protein2 (Arp2) and similar proteins
Arp2, also called actin-like protein 2, is the ATP-binding component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex is comprised of 7 proteins (Arp2, Arp3, and five conserved proteins, ARPC1-5). It generates cytoplasmic branched filaments networks, by promoting nucleation of actin filaments as 70 degrees branches on the side of older filaments. It is activated, by simultaneously binding to a pre-existing filament and a nucleation promoting factor plus an actin monomer. Daughter branches subsequently detach/debranch from the mother filament. Its Arp2 and Arp3 subunits must be loaded with ATP for it to initiate the assembly of branched actin filaments. ATP hydrolysis may be required for branch initiation or debranching. The Arp2/3 complex is also found in the nucleus where it plays a role in promoting de novo actin polymerization and in RNA polymerase II-dependent transcription. This may in part be through regulating nuclear actin polymerization in a way like its function in the cytoplasm. Human Arp2 is encoded by the ACTR2 gene.
Statistics
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PSSM-Id: 466821
Aligned: 67 rows
Threshold Bit Score: 631.906
Created: 9-Oct-2006
Updated: 27-Apr-2023
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 21 residues -Click on image for an interactive view with Cn3D
Feature 1:nucleotide binding site [chemical binding site]
Evidence:
  • Comment:these Bos taurus ARP2 structures are missing some coordinates
  • Structure:2P9K; Bos taurus ARP2 binds CaATP, contacts at 4A
  • Structure:2P9I; Bos taurus ARP2 binds CaADP, contacts at 4A
  • Structure:6DEC; Bos taurus ARP2 binds CaATP, contacts at 4A

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1            #### #                                                                   
4JD2_B      7 KVVVCDNGTGFVKCGYAGsNFPEHIFPALVGRPIIRSttkv-----------gnieIKDLMVGDEASELRSmLEVNYPME 75  cattle
2P9K_B      7 KVVVCDNGTGFVKCGYAGsNFPEHIFPSLVGRPIIRSttkv-----------gnieIKDLMVGDEASELRSmLEVNYPME 75  cattle
CAH69755    3 NPIIEDSGTGYLKIGFAGdNFPSHSFPAMVGRPTLRAdeql-----------ddanLKDIMIGDEAQPYRGlLELTHPLE 71  Paramecium tetraur...
CAL57082   22 NVVVCDTGTGFVKAGYAGdEAPRVVFPCVTGRPTLRYeeama----------seeaMKDIYVGDEAARRRAnLEISYPVS 91  Ostreococcus tauri
EIE26597   13 RVIVCDNGTGFVKCGFAGdISPRAVFPCVVGRPINGLad----------------tGQKVFVGNDCTNRRHeLDISYPVS 76  Coccomyxa sp. C-169
ESL11944    6 QPVVLDNGSGFIKCGFAGaNFPTAVFQTSLGRPVLRStkhrnhnggvgghthvdptMCDLIFGDDCSGVRHlLDMTYPIR 85  Trypanosoma rangel...
AIZ74321    5 KVIVCDNGTGFVKCGFEGgNFPAATFPCIIGKPVLRFeetl-----------cdqeLKDIVVGEDCSRLRNqLEVSYPIK 73  Pinus massoniana
GAQ89930  128 KVVVCDNGTGFVKCGFAGnNFPSAVFPCVVGRPILRYeeaq-----------gqeaLEDVVVGARASALRAqLEVSYPVQ 196 Klebsormidium nitens
KXZ54874    8 RVVVCDNGTGLVKCGFAGdSFPRAVFPCLVGRPTFRYndpfs----------qqqqLKDMYVGSEAATHKAqLELSYPMR 77  Gonium pectorale
OHT05637    5 CPIVLDLGTGFLKAGFSVnPIPDFVIPNSVGRPILRSdesf-----------srakIKDVMICDEITPVRQyLDLTFPVE 73  Tritrichomonas foetus
Feature 1                                                                                     
4JD2_B     76 NGIVRNWDDMKHLWDYTFGpek--lnIDTRNCKILLTEPPmnPTKNREKIVEVMFEtyqfSGVYVAIQAVLTLYAQGLLT 153 cattle
2P9K_B     76 NGIVRNWDDMKHLWDYTFGpek--lnIDTRNCKILLTEPPmnPTKNREKIVEVMFEtyqfSGVYVAIQAVLTLYAQGLLT 153 cattle
CAH69755   72 EGVVKNWEDMELIWDYGYKkl----gIDPKGQTVLMTEAAgnPKKNREKMAEIKFEkfgfGQLNVGIQALLPLFAEGLRT 147 Paramecium tetraur...
CAL57082   92 NGVVRDWDDMGLVWDHAFErl----gCTPEDCKIMLTDPPlnPKANRERMMSTMFEtygfRAAYVQVQAVLTLYAQGLMT 167 Ostreococcus tauri
EIE26597   77 NGLVQSWEDMQHVWDHTFEdvlrmdsHARAESRILLTEPPlnPISNRHRLLETMFEtynfAGAQVQIQAVLTLYAQGLVT 156 Coccomyxa sp. C-169
ESL11944   86 NGIIQNMDEMCLLWDYTFGer---lqIDPTEHRLLLSEVPlfSSRHRCHLYEVMFEnynfTAIQSAVQGTLSLFSNGLQT 162 Trypanosoma rangel...
AIZ74321   74 NGIIQDWEGMGHVWDHMLYel---lkAEPSECKILLSDPPlnPTRNRQKLVQTMFEtynfSGLSIQINAILTLYAQGLLT 150 Pinus massoniana
GAQ89930  197 NGIVQSWEDMGHIWDHTFYdk---lrIDPSECRILLTDPPlnPSKNREKMIETMFEkygfAGAFIQIQAVLTLYAQGLLT 273 Klebsormidium nitens
KXZ54874   78 NGIVQSWDDMGLIWDHAFGpgq--lgVDPRECRILLTDPAmnPTANRQRMLEVMFEtygfAGANMQIQAVLTLYAQGLLT 155 Gonium pectorale
OHT05637   74 HGVIKNWEDETLVLDYLFKnk---lkINNEDHPVLITEAPlnPLENRKKIFEVFFEtfnfPAIQMAPQALLVLYAQGLVT 150 Tritrichomonas foetus
Feature 1         # ####                      ##                          #  ##               
4JD2_B    154 GVVVDSGDGVTHICPVYEGFSLpHLTRRLDIAGRDITRYLIKLLLLRGYAfNHSADFETVRMIKEKLCYVGYNIEQEQKL 233 cattle
2P9K_B    154 GVVVDSGDGVTHICPVYEGFSLpHLTRRLDIAGRDITRYLIKLLLLRGYAfNHSADFETVRMIKEKLCYVGYNIEQEQKL 233 cattle
CAH69755  148 ALLLDAGDGVTHCMPVYDGFCLnQGAQRMNIAGRHVTDQLVKLLFQRGYAfNSSADFELVREIKEALCFVSSDIRMDTKL 227 Paramecium tetraur...
CAL57082  168 GIVVDSGDGVTHIVPVVDGFSFpHLTKRLNVAGRHVTTRMVDLLTRRGYPlNRSADMETARLIKEDLCYVAYDYKRDLQL 247 Ostreococcus tauri
EIE26597  157 GMVVDVGDGVTHIIPVIEGCSFpHLTKRLNVAGRDVTARLVDLLQRRGYAlNRSADFDTVRDVKEQLCFVSADCKRDLQL 236 Coccomyxa sp. C-169
ESL11944  163 GVAVESGEGISHCTPIFEGYALpRANRRVDLGGRNITEFLIRLMQRRGYSfNQSSDYETVRRMKERFCYAAVDTGLEKQL 242 Trypanosoma rangel...
AIZ74321  151 GLVVDSGDGVTNIVPVIDGYSFpHATKRVNIAGHHITSYLIELLLRRGYAlNRTSDFETAREIKEQLCYISYDFKREIQL 230 Pinus massoniana
GAQ89930  274 GLVVDSGDGVTHVVPVVDGFAFpHLTKRMNVAGRHITAHLIDLLSRRGYAfNRTADFETVREIKEQLCYVSADYAREMRL 353 Klebsormidium nitens
KXZ54874  156 GLVVDSGDGVSHAVAVVDGYAFpHQTKRLNVAGRHVTARLLELLQRRGYAlNAAADLDSVRAIKEQLAYVAFDPAREMQL 235 Gonium pectorale
OHT05637  151 GVVVDSGDGVTHIMPIFENYLLsHLIGRMDIAGRDITEHMVKLLTLRGYTfHKSSDFDVVREIKEKMCFVSADINVDRRI 230 Tritrichomonas foetus
Feature 1                                                                            ### ##   
4JD2_B    234 ALETTVLVESYTLPdGRIIKVGGERFeAPEALFQPHLINvegvGVAELLFNTIQAaDIDTRSEFYKHIVLSGGSTMYPGL 313 cattle
2P9K_B    234 ALETTVLVESYTLPdGRIIKVGGERFeAPEALFQPHLINvegvGVAELLFNTIQAaDIDTRSEFYKHIVLSGGSTMYPGL 313 cattle
CAH69755  228 AQETTCHESTFRLPdGGRVKIGQERYmAPEILFSPWLLGkdapGCADIVFNAIQKsPIDARKTFYENILISGGTTMFPGF 307 Paramecium tetraur...
CAL57082  248 ARETTATNASYTLPdGRVIKFGPERFmGPECLFQPDLIDvegdGISDLVFKCIQEnEIDNRRALYQHIVLSGGNSMYAGL 327 Ostreococcus tauri
EIE26597  237 ARETTCQQRAYTLPdGRTIRVGSERFmAPEALFNPSLLDvespGIAEQVFQCIQEmDVDNRASLYQHIVLSGGSTMLPGL 316 Coccomyxa sp. C-169
ESL11944  243 ALETTVLEQNFLLPdGSSCSIGQERFeATEALFQPHLIDiecdGLSTQLWNCIQAaDIDVRAQLYSHVVLSGGSTMFPGL 322 Trypanosoma rangel...
AIZ74321  231 GTETTVLVKDYMLPdGRVIRVGTERFqAPEALFNPELIDiegaGLADLVFQCIQGmDIDHRLVLYQHIVLNGGSTMYPGL 310 Pinus massoniana
GAQ89930  354 AQETTILVKNYTLPdGRVISVGAERFqAAEALFRPELVDcegeGLSGLVFRCIQEmDIDNRMPLYQHIVLSGGSSMYPGL 433 Klebsormidium nitens
KXZ54874  236 ARETTTLMRSYTLPdGRVVRLGAERFlAPEVMFNPRLLDmeapGIAEMAFNAIQDaPIDNRRGLYERIVISGGSTMYPGM 315 Gonium pectorale
OHT05637  231 ANETTTYVVPYVLNdGRIIKVSGERFeAPEVLFQPELLGiegnGIHQLLFDTIMKsDINLRKEFFKSIVVSGGTTMYPGF 310 Tritrichomonas foetus
Feature 1                                            #                                     
4JD2_B    314 PSRLERELKQLYLeRVLKGDVEKls--kFKIRIEDPPRRKHMVFLGGAVLADIMKDKdnFWMTRQEYQEKGVRVLEK 388 cattle
2P9K_B    314 PSRLERELKQLYLeRVLKGDVEKls--kFKIRIEDPPRRKHMVFLGGAVLADIMKDKdnFWMTRQEYQEKGVRVLEK 388 cattle
CAH69755  308 PTRLQNQLRTIFEeTVLKGNTAYaanskIKIKVLDPARRKYNVFIGASFLSNVMKDKknFWISKEDWEEQGAERAMM 384 Paramecium tetraurelia
CAL57082  328 PSRLERDIKRLYLkNVLDGDREAmk--kFKLKVEAPAHRKHMVFVGGAVLADIMRSKdeFWISKEEYEEQGIERALK 402 Ostreococcus tauri
EIE26597  317 TSRLDRDIKALYLqRVLKGDKEGlr--nFKLKIDNPPLREHIVFSGGSVLADIMQSQdsFWVSRQEWQEDPHRAMKK 391 Coccomyxa sp. C-169
ESL11944  323 PTRVEKDMRALFLeKALKGDRSRls--hFKLQIEDPPRRKHMVFLGGATLAALSAEQpeMWLTRTEWQEGGVSAVRA 397 Trypanosoma rangeli SC58
AIZ74321  311 PSRLEEEVRLCYLnNVLQGNKQGlk--kFRLRIEDPPSRKYMAYLGGSVLAGIMKDEphFWISRQEYEEEGLRCLQK 385 Pinus massoniana
GAQ89930  434 PSRLEADLRARYLqHVLKGNTEAlk--kFRLRIEDPPRRRHMVFLGGAVLADIMKDKpeFWISKQEYEEEGVRCLKK 508 Klebsormidium nitens
KXZ54874  316 STRMDKEMRALYVeRVLKGDMAGlr--kLKLHIADPPNRKHMVFLGGAVLADIMKDHtdFWITRQQYAEDPAGALRK 390 Gonium pectorale
OHT05637  311 STRLENELRRLVLnNVLKGDTKAlk--sYKISVEDPPNRRFLVYLGATVLANLSKEKtqSWAQRTAYKEAGPEQLAR 385 Tritrichomonas foetus

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