Conserved Protein Domain Family
APS_reduc
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TIGR00424: APS_reduc 
5'-adenylylsulfate reductase, thioredoxin-independent
This enzyme, involved in the assimilation of inorganic sulfate, is closely related to the thioredoxin-dependent PAPS reductase of Bacteria (CysH) and Saccharomyces cerevisiae. However, it has its own C-terminal thioredoxin-like domain and is not thioredoxin-dependent. Also, it has a substrate preference for 5'-adenylylsulfate (APS) over 3'-phosphoadenylylsulfate (PAPS) so the pathway does not require an APS kinase (CysC) to convert APS to PAPS. Arabidopsis thaliana appears to have three isozymes, all able to complement E. coli CysH mutants (even in backgrounds lacking thioredoxin or APS kinase) but likely localized to different compartments in Arabidopsis. [Central intermediary metabolism, Sulfur metabolism]
Links
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Statistics
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PSSM-Id: 273072
Aligned: 3 rows
Threshold Bit Score: 946.759
Created: 7-Oct-2014
Updated: 25-Oct-2021
Structure
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Aligned Rows:
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Sequence Alignment
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Format: Row Display: Color Bits: Type Selection: 
AAC49561   1 MAMSVNVSSSSSSGIINSRFGVSLEPKVSQIGSLRLLDRVHvapvslnLSGKRSSsVKPLNAEP--KTKDSMIPLAATmV 78  thale cress
AAB57688   1 MALAVTSSSTAISGSTFSRSGASSESKALQICSIRLSDRNH-------LSQRRYS-MKPLNAESshTRSESWVLRAST-L 71  thale cress
AAB05871   1 MALAFTSS-TAIHG-SLSSSFEQTKAAAAQFGSFQPLDRPHtispsvnVSRRRLA-VKPINAEP--KRNESIVPSAAT-T 74  Madagascar periwinkle

AAC49561  79 AEIAEEVEVVEIEDFEELAKKLENASPLEIMDKALEKYGNDIAIAFSGAEDVALIEYAHLTGRPFRVFSLDTGRLNPETY 158 thale cress
AAB57688  72 IAPEVEEKEGEVEDFEQLAKKLEDASPLEIMDKALEKFGDEIAIAFSGAEDVALIEYARLTGKPFRVFSLDTGRLNPETY 151 thale cress
AAB05871  75 VAPEVEEK-VDVEDYEKLADELQNASPLEIMDKSLAKFGNDIAIAFSGAEDVALIEYAHLTGRPFRVFSLDTGRLNPETY 153 Madagascar periwinkle

AAC49561 159 RFFDAVEKHYGIRIEYMFPDSVEVQGLVRSKGLFSFYEDGHQECCRVRKVRPLRRALKGLKAWITGQRKDQSPGTRSEIP 238 thale cress
AAB57688 152 RLFDAVEKQYGIRIEYMFPDAVEVQALVRNKGLFSFYEDGHQECCRVRKVRPLRRALKGLKAWITGQRKDQSPGTRSEIP 231 thale cress
AAB05871 154 KFFDTVEKQYGIHIEYMFPDAVEVQALVRSKGLFSFYEDGHQECCRVRKVRPLRRALKGLRAWITGQRKDQSPGTRSEIP 233 Madagascar periwinkle

AAC49561 239 VVQVDPVFEGLDGGVGSLVKWNPVANVEGNDVWNFLRTMDVPVNTLHAAGYISIGCEPCTKAVLPGQHEREGRWWWEDAK 318 thale cress
AAB57688 232 IVQVDPVFEGLDGGVGSLVKWNPLANVEGADVWNFLRTMDVPVNALHAQGYVSIGCEPCTRPVLPGQHEREGRWWWEDAK 311 thale cress
AAB05871 234 VVQVDPVFEGMDGGVGSLVKWNPVANVEGKDIWNFLRAMDVPVNTLHSQGYVSIGCEPCTRPVLPGQHEREGRWWWEDAK 313 Madagascar periwinkle

AAC49561 319 AKECGLHKGNVKEns----dDAKVNGEsKSAVADIFKSENLVTLSRQGIENLMKLEFRKEPWIVVLYAPWCPFCQAMEAS 394 thale cress
AAB57688 312 AKECGLHKGNIKEe------DGAADSK-PAAVQEIFESNNVVALSRGGVENLLKLENRKEAWLVVLYAPWCPFCQAMEAS 384 thale cress
AAB05871 314 AKECGLHKGNIKEetlnnngNGAVNGNgSDTIADIFDTNNVTSLSRPGIENLLKLEERREAWLVVLYAPWCRFCQAMEGS 393 Madagascar periwinkle

AAC49561 395 YDELAAKLAGSGDKVAKFRADGDQKEFAKQELQLGSFPTILVFPKNSSRPIKYPSEKRDVESLTSFLNLVR 465 thale cress
AAB57688 385 YVELAEKLAGKGVKVAKFRADGEQKEFAKQELQLGSFPTILLFPKRAPRAIKYPSEHRDVDSLMSFVNLLR 455 thale cress
AAB05871 394 YLELAEKLAGSGVKVGKFKADGDQKAFAQQELQLNSFPTILFFPKHSSKPIKYPSEKRDVDSLMAFVNALR 464 Madagascar periwinkle
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