3MZK


Conserved Protein Domain Family
ACE1-Sec16-like

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cd09233: ACE1-Sec16-like 
Click on image for an interactive view with Cn3D
Ancestral coatomer element 1 (ACE1) of COPII coat complex assembly protein Sec16
COPII coat complex plays an important role in vesicular traffic of newly synthezised proteins from the endoplasmatic reticulum (ER) to the Golgi apparatus by mediating the formation of transport vesicles. COPII consists of an outer coat, made up of the scaffold proteins Sec31 and Sec13, and the cargo adaptor complex, Sec23 and Sec24, which are recruited by the small GTPase Sar1. Sec16 is involved in the early steps of the assembly process. Sec16 forms elongated heterotetramers with Sec13, Sec13-(Sec16)2-Sec13. It interacts with Sec13 by insertion of a single beta-blade to close the six-bladded beta propeller of Sec13. In the same way Sec13 interacts with Sec31 and Nup145C, a nuclear pore protein, all of these contain a structurally related ancestral coatomer element 1 (ACE1). Sec16 is believed to be a key component in maintaining the integrity of the ER exit site.
Statistics
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PSSM-Id: 187750
Aligned: 55 rows
Threshold Bit Score: 168.205
Created: 10-Feb-2011
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
homodimerheterodimer
Conserved site includes 25 residues -Click on image for an interactive view with Cn3D
Feature 1:homodimer interface [polypeptide binding site]
Evidence:
  • Structure:3MZK, Yeast Sec16 homodimer, contacts 4A
  • Comment:Sec16 forms an elongated heterotetramers (dimer of heterodimers) with Sec13, Sec13-(Sec16)2-Sec13

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1                                                                                         
3MZK_B         66 FPGPLgsak-----lkkkDLTKWMETTIKSISenes-------stdmTIWQLLEMKLnd------kVNWKNISKllynsd 127  baker's yeast
XP_455029     942 FPGPLikgk-----skskDIAKWLDTYIEEKKlkga-------ndtcILLELLKLRLdps----ysLKAVTNIIydssil 1005 Kluyveromyces...
XP_001386126 1003 FPGPLsksk-----tkrkDVEKWLESNIALLTtnsie-----nqdeyLLNQVLLEAVkfe----ggFNSHEFIKavcmvl 1068 Pichia stipit...
AAZ20113     1091 FPGPLtkgk-----akqkELTKWIEQKIASYNessdvl----seqksLLWKVLLYSVqkld-enpeVFNKNLVQlldpqt 1160 Pichia pastoris
XP_002615459 1305 FPGPLtksk-----skkkDIETWLEKNIKSLEakqa-------tdelLLAKVLLALLrhdsapnnsMLNQNVASvlnpql 1372 Clavispora lu...
XP_457088     972 FPGPFnklk-----tkkkDVEKWLESYNEFLIqnntgm----kqdevLVSQILLALVrfn----gdCKSDDFTKaacavl 1038 Debaryomyces ...
A5DH19        796 FPGPLskks------kakDVEKWLKTKVTHLDsstpnvatneaklsdEVGEILAALVasn----gdVRSQEFIStacsvl 865  Pichia guilli...
XP_002171572 1226 FQGPYigsngkidkakrkQALAWVEKRLHYLEdqgen------eeklYIYKALGLMLgtd---dkhRIRDGVREmllpnf 1296 Schizosacchar...
XP_002554044 1189 FPGPLvknk-----tktkDVDKWIADACTELSalss-------lkdiCIWKILKLKLsse----stMKDISHALydsdel 1252 Lachancea the...
XP_001644733 1107 YPGPLdsfk-----ksktQLSSWLSVVLENLTtts---------tfsVLISLFKIWIdss----nvVQDIIHILsdenkl 1168 Vanderwaltozy...
Feature 1                                            ## ## ##### # #         # # ## ##   #     #  
3MZK_B        128 ellmylsqp-----------fpngdmipnayrldINCQMRVLAFLQtGNHDEALRLALSKRDYAIALLVGSLMGKDRWSE 196  baker's yeast
XP_455029    1006 lpylsqpf-------------ipkvaapnahkldANSLIRVLTLLQtGAHDDALNLSLALKDYAMALLLGSILGKEKWSS 1072 Kluyveromyces...
XP_001386126 1069 nptadyntpg--------disgmtsvsanaykldNAGIGIVFGLIQgGHIDKALEFTLSKGDWALALVVSNFAGHDRFAK 1140 Pichia stipit...
AAZ20113     1161 qvspdepidynal---tksletathrranasaldPTELTYLYGLLQtGEKQRALSYSIFNRDWAMSLLLSSLIAPEQYAS 1237 Pichia pastoris
XP_002615459 1373 dysteekpqv--------sfmmqssvtsnaskldNAAISTIASMLQvGDRENAMNFSISRQDWALSLVIAHSISPEKFAQ 1444 Clavispora lu...
XP_457088    1039 npsvdyandnt------hmdmnpistlanayrldNTGINIIWSLLQiGNTEKALEFCLSKGDWALGLMIARFEGPEKFGK 1112 Debaryomyces ...
A5DH19        866 nphhysqveam-------atpqgsnhsataykldNSGSNQLLTYCQaGQTDRALELCVAAGDWALALIISHSMGPQAFGK 938  Pichia guilli...
XP_002171572 1297 vppetknstktvaelmnpsldedntpvvatssvtAGFLQKIHNFLLvGDRESALTCAMNRKNWAYAILLASSLGKETFQS 1376 Schizosacchar...
XP_002554044 1253 lpflsqps-------------smkkggfnsnkldENSQLKVLAALQtGNHLQALDLALSQRDFALALILGSLLGKDKWSD 1319 Lachancea the...
XP_001644733 1169 qeafdnieh----------kktiseskrdsrildTNGKDKILKLLLlGKKNEALEFSMENSDFTMALLIANTIDEASRHS 1238 Vanderwaltozy...
Feature 1             #                  #    #  #                                          ##    
3MZK_B        197 VIQKYLyegftag-pndqkelAHFLLLIFQVFVGnsKMAIKsfytnnet---------sqwasenWKSIVAAVLINipen 266  baker's yeast
XP_455029    1073 VVDQYLtqefeqs-nerdissINLLATIFQVFIGnsKKVIDsfkqdptk---------lewslnnWKLLIACILNNceev 1142 Kluyveromyces...
XP_001386126 1141 VAADYArfsfpyq--ksnnkiHHIIPILLKLAVGnvKSVIDdlnavate---------geyashhWREIVSSVIISgvsk 1209 Pichia stipit...
AAZ20113     1238 TVKLFLkyn---------fneHDPVSKKLTFFLGl-SNLADaedlkhh----------stfiiknFESLLTFILNNkpn- 1296 Pichia pastoris
XP_002615459 1445 VASHYSkamfplh-knthnktYHLMSIMLRCFAGntKSLVEsfsvdpne---------tefakqsYREIIAAALVNgvt- 1513 Clavispora lu...
XP_457088    1113 VASDYArttfpfq--ksqskvHHLMPIMLKLFAGnfKSAIDditnvqae---------gewfiqnWRELVSLVVINkpqh 1181 Debaryomyces ...
A5DH19        939 TASDFArtsypfq--ksqsklNHLMPIILKVFSGnvKSIIEdfqnvate---------lewvllhWRDVVTSIAANaiqn 1007 Pichia guilli...
XP_002171572 1377 VVHDFIksdlmse--tqtanvEVNLRVIFQLLSGa-SLAFSelssscpllsltqansaegnqdlqWKELLCMLLANdvpe 1453 Schizosacchar...
XP_002554044 1320 VVDIYLheefqssnegggsfsVNLLALIFQVSVGnsRRVIKelsvnpsk---------elwamqnWKMIISAILNNvnhq 1390 Lachancea the...
XP_001644733 1239 VIMSYFenkinpv-ntekksiNMLLLALFQVYSGdgTHLLNiakenpet---------kdwyvknWNSIVLILLKNcdmn 1308 Vanderwaltozy...
Feature 1                                                                                         
3MZK_B        267 nedpl----lippvvleFLIEFGIFLTKkgltaAASTLFIIGnvplsnepvmads--------dvifesignmntfESIL 334  baker's yeast
XP_455029    1143 pang-----klspivveFLLQFGELLVSrglviAGSIAFIVAdipmseeplipfs--------sttfeyiccpnsfESLI 1209 Kluyveromyces...
XP_001386126 1210 --------------sqdFLVEFGKFLGLhhniaASEICYVIAglplspqalqps-----------givvsvigsltGTSM 1264 Pichia stipit...
AAZ20113     1297 --------------penILLLIGNTLTEvgfftYAQIVFAMSgvplipne------------------shvatrdiESII 1344 Pichia pastoris
XP_002615459 1514 ---------------geFFIEYGGFLASagitcGSEICYMLAgvimsrvplqn------------gnvflsagsftQSLV 1566 Clavispora lu...
XP_457088    1182 --------------gheFLCEFSKLLALsgqiiASQICLILAglplssipsqan-----------gilfsvigfgsHSFA 1236 Debaryomyces ...
A5DH19       1008 g------------kvneFLNEFGQLLAShgnfiGRDVCFILTgaplspsf------------------svvggdsqMGLF 1057 Pichia guilli...
XP_002171572 1454 --------------qvqAFRHLGELLLQqgrtyAAHLVFLLSmspiiclyngkernpmplvglvnpnaysdstellESVM 1519 Schizosacchar...
XP_002554044 1391 hendsaktqklpllcleFLVEFGVFLCQrgmviEGFTSFVIAdvplsvhevipgs--------gvrfqtvgsmnssESYF 1462 Lachancea the...
XP_001644733 1309 agniss-kpnshqkvtsFLVGFSNFLRNtgnilPSFIVCLISdmlnsgsengiev---------dmldsvfgkhtvSSSI 1378 Vanderwaltozy...
Feature 1                                                                                         
3MZK_B        335 WDEIYEYIfsydpk------fkgfssILPQKIYHASLLQEQGlnslGTKYTDYLSSSVrklp--kkdiLTINLTRELSEV 406  baker's yeast
XP_455029    1210 LSEIYEFTygqkdps-----fsgftsLIPQKIIHAAGLSDYGlnssSTKYMDMAQSMLkslp--knseTTLKTLRYIEDL 1282 Kluyveromyces...
XP_001386126 1265 YTEVYEYIlkistvfvq-qgvgiiphLLPLKLKHATVLADYGlfneSQKYIDSINNNMktlgn-rspyLNAAFIHELQSL 1342 Pichia stipit...
AAZ20113     1345 FNEVYEWIiqnkpnsa---fvngipqLVVMKAIHASILSDIGavaeAEKYCNYALQSLkntk---tviSNQVLLHELQSL 1418 Pichia pastoris
XP_002615459 1567 YTEIYEYIlqmspmtsnvipptgiphLIEFKIHRAQVLADLGhfttSKKYCDAINSTLkvlg--ksplVSAQALAEFQTL 1644 Clavispora lu...
XP_457088    1237 YSETYEYAmqlsttni---pptgfahLLPLKLKHAQVLADYGlfteSQKYCDAISSIIkatg--rssfFNPVAFQEFQNL 1311 Debaryomyces ...
A5DH19       1058 YTEIYEYAlsqrsaa----tantllpTLSVKLHHAQLLADYGlnvdSQRYCDQIGASLktt----kqqVPPAIIHEFQRL 1129 Pichia guilli...
XP_002171572 1520 LTEVWELIlnlhank----etisyahLQIFKLYHARVLADYGqlalAKRYCECIGQYLkmaskkssasIDPSLVSGVRDL 1595 Schizosacchar...
XP_002554044 1463 LSEIYEYLhsladpk-----fsgfdhLLLQKVAHAAALTGYGltsaASRYTDQLALSLkslp--knshIAHTVSARLNFV 1535 Lachancea the...
XP_001644733 1379 ISEIYSLIkisknq------nsdeflLYSSSLECARLFFDIGyhskALEYVNYFVSIKdrli--aneiFVDTSLEQLKVI 1450 Vanderwaltozy...
Feature 1                 
3MZK_B        407 ASRLSESN 414  baker's yeast
XP_455029    1283 SNQLSSSN 1290 Kluyveromyces lactis NRRL Y-1140
XP_001386126 1343 IVRLQELG 1350 Pichia stipitis CBS 6054
AAZ20113     1419 SLLLSEAG 1426 Pichia pastoris
XP_002615459 1645 VMRLSETS 1652 Clavispora lusitaniae ATCC 42720
XP_457088    1312 LMRISQSG 1319 Debaryomyces hansenii CBS767
A5DH19       1130 LVRVSDTG 1137 Pichia guilliermondii
XP_002171572 1596 SRQVLESS 1603 Schizosaccharomyces japonicus yFS275
XP_002554044 1536 SSKLTGTN 1543 Lachancea thermotolerans CBS 6340
XP_001644733 1451 SELVNPGH 1458 Vanderwaltozyma polyspora DSM 70294

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