Conserved Protein Domain Family
ALG3

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pfam05208: ALG3 
ALG3 protein
The formation of N-glycosidic linkages of glycoproteins involves the ordered assembly of the common Glc3Man9GlcNAc2 core-oligosaccharide on the lipid carrier dolichyl pyrophosphate. Whereas early mannosylation steps occur on the cytoplasmic side of the endoplasmic reticulum with GDP-Man as donor, the final reactions from Man5GlcNAc2-PP-Dol to Man9GlcNAc2-PP-Dol on the lumenal side use Dol-P-Man. ALG3 gene encodes the Dol-P-Man:Man5GlcNAc2-PP-Dol mannosyltransferase.
Statistics
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PSSM-Id: 461587
Aligned: 137 rows
Threshold Bit Score: 420.728
Created: 22-Mar-2022
Updated: 17-Oct-2022
Structure
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Aligned Rows:
PubMed ReferencesClick to see Conserved Features Help

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
WGS:AAPU:GI19772-PA  49 LLIVLAEAVINVLVIQHVPYTEIDWVAYMQECEGFLNGTTNYALLRGDTGPLVYPAAFVYIYSALYYL------------ 116
XP_003063992          3 RVLVVLDALLSVAIVRRVAYTNIDWNAYMGEARGYASGERDYAKLRGDTGPLVYPAGFLYLYDALRRV------------ 70 
jgi:OSTLU_47741      24 VALIAFELALCAVIIAKVAYTEIDWIAYMEEVHGYARGERDYTKLKGGTGPLVYPAGFVYAYKALYDL------------ 91 
XP_001568847          5 nvALAAETVVIIIVILVVPYTEIDWRAYMEEVEGFLNGELDYRNLKGGTGPLVYPGGFIWVHAVLYYL------------ 72 
Q4Q1L8                5 dvaLAAEAVVIITVILTVPYTEIDWKAYMEEVEGFLAGELDYRNLKGGTGPLVYPGGFVWVYSVLYYL------------ 72  Leishman...
Q4E4B0                7 nALLAAEVLISLIVVLFVPYTEIDWNAYMQEVKGFLEGELDYRKLRGDTGPLVYPAGFVWIYSALYFI------------ 74  Trypanos...
Q38AV3                7 nrVLTVELCMSIIAFHLVHFTGIDWNAYMQEVKGFLDGELDYMKLKGDTGPLVYPGGFVWSHSALYFM------------ 74  Trypanos...
CBJ29699             33 agaVLAEIALCGIIIARIAYTEIDWVAYMQEVKGFLDGERNYLNLRGDTGPLVYPAGFVYAYSLLYWV------------ 100
XP_002178258          1 --LLIMELGLNVVVIRKVAYTEIDWEAYMQEVEFWLDGELDYRKIYGGTGPLVYPAGFLYLFAWLRKL------------ 66 
EED91209              1 -iLIVVEVILTTLIVIRVPYTEIDWVAYMQEVTTYQNGERDYANIRGDTGPLVYPAGFLYLYGWLKSVatksdlnkddpl 79 
WGS:AAPU:GI19772-PA 117 ----T---AHGS---NVRLAQYIYTGIYLLQMCLALRLYAKs--RKVPPYMLV------------------------LTA 160
XP_003063992         71 ----T---DDGA---DVRRAQMIFVVVYAAHQAAAMAVTVKa--RVVPPWALS------------------------LLC 114
jgi:OSTLU_47741      92 ----VgglERGTsarGVATAQVVFALVYAAHQALVFSMYAMc--EIIPPWAYA------------------------LLC 141
XP_001568847         73 ----T---KKGE---AIELAQLIYASVYLVTLAMVLRLYSH---SGHT-WRVI------------------------IPL 114
Q4Q1L8               73 ----T---KKGE---AIELAQWIYAGVYLVTLTIILRLYTR---SGLT-WKTM------------------------IPL 114 Leishman...
Q4E4B0               75 ----T---KEGK---DIEMAQWLYAGIYIMLFACVIQLYVR---CKIR-GRLL------------------------IRL 116 Trypanos...
Q38AV3               75 ----T---KGGV---DVEMAQWLYLGIYVMVLVLVAHLYYN---SGLR-GRLF------------------------IRL 116 Trypanos...
CBJ29699            101 ----T---DRGE---DIFTAQYIFMGVYVSLMVMVLSIYRRagsGAMPLWSIL------------------------LVC 146
XP_002178258         67 ----T---NSGN---DIQTAQYIFAAFYVGTQFAVLLLYQQ---------ILSswl------------------gmaVLC 109
EED91209             80 gldgS---TSPE---AVRRIQWVFVVCYVFNCMVVLALYQK---------VLQrlrqqgmqsstsiamvwcwrigmgLTC 144
WGS:AAPU:GI19772-PA 161 FtSYRIHSIYVLRLFNDPVAVLLLYASLNLFM---D------SRWLWGTIFYSLAVGVKMNILLFAPALLL-FYLANLG- 229
XP_003063992        115 V-SKRIHSVFALRMFNDGVCSLVAWVAIWLAQ---S------KRFVAAAVALSVAASVKMNALLFLPPFLV-LLVGHER- 182
jgi:OSTLU_47741     142 L-SKRVHSIFVLRMFNDGVAMALAYGATTLFQ---R------KRWIAGSVVFSLGVSVKMNVLLMLPGLLV-LLVGGTS- 209
XP_001568847        115 FiSKRIRSLYVLRLFNDCWAMLFLFAAVTFIAgrpSgagkrsRQWLIGSICFTIAVSIKMNVLLFAPGMLY-VMLRTLP- 192
Q4Q1L8              115 FvSKRIRSLYVLRLFNDCWAMLFLFAAVSFIAgrpSgaakrsRQWFIGCLCYSIAVSIKMNVLLFAPGMLY-VMLRTLP- 192 Leishman...
Q4E4B0              117 LlSKRIRSLFMLRLFNDCVAMLLLYFAVVVLA---Kg-----RRWKLGCLLYSMAVSVKMNILLFAPGLLL-ILCRSLP- 186 Trypanos...
Q38AV3              117 LlSKRIRSLFMFRLFNDCWAMFLVYLSVICFA---Rg-----RRWTVGCLLYSMAVSVKMNIFLFAPGLLL-ILCKSLP- 186 Trypanos...
CBJ29699            147 T-SRRVHSIFVLRLFNDGVAMWLLYGAVYLFL---L------NRWRLGCVSYSLAVGVKMNILLFAPGLLL-LLVQANG- 214
XP_002178258        110 L-SKRIHSIFVLRLFNDGPTMLLLYISMLLFT---W------NRWNVGCVVFSMAVSIKMNVLLFAPGLLLlLLQVGPDa 179
EED91209            145 L-SKRIHSIFVLRLFNDAPSMILLHVSMYLFA---Cc-----DAWTLGCVVFSLAVSIKMNVLLFAPGLLL-LLLQKNQs 214
WGS:AAPU:GI19772-PA 230 VLLTIVHLFICGLIQVVIAYPFLRTHPVEYLTGSFDLGRIFEHKWTVNYRFLTRELFEQREFHLALLGLHLLLLLAFAky 309
XP_003063992        183 VSTACVAVGAFVGVQVVVGWPFLSTYPREYVGRAFELGRKFTYEWTVNWKFVSIETFSSDAFSLGLLLAHVAILFAFA-- 260
jgi:OSTLU_47741     210 AGTAIGAVAAIVGTQIALGAPFLATYPREYLSRAFELGRVFTHKWSVNFKFVSEDIFVSKSFAKYLLLAHLVCLFVFA-- 287
XP_001568847        193 LRRVAWYLFICAVWQVVAGIPFVAHNYKAYLSKSFELDRVFMHRWSLNYQFLDAELFTKPGFGQALLAMTAATWVLLW-- 270
Q4Q1L8              193 FGRVAWYLLVCAAWQVAAGLPFLAYDYRAYLSKSFELDRVFLQRWSVNYQFLNAEIFVKPEFGQALLAMTVATWVLLW-- 270 Leishman...
Q4E4B0              187 WYRVILCLVVCALWQVGVALPFLLHELQAYMLKAFELGRVFTYRWTVNFKCIPEEIFISKEFGQVLLLFTVVSWFLLW-- 264 Trypanos...
Q38AV3              187 FTGVVRCLAVCALWQVVAGLPFLLHNPRSYIVRSFDLGRVFTYRWTVNFKRISEEIFFSSKFSRSLLVMLAVSWLLVC-- 264 Trypanos...
CBJ29699            215 VGGAAVCLSICAGVQLLLGAPFLLHHPVAYLSRSFELGRVFKFEWTVNFKFLTEEAFVSPGLSIALLALTLAVLALFA-- 292
XP_002178258        180 QTVALRLGLGCALPQLILGAPFLTTYPVSYLRKAFELDRVFFYKWTVNFKFLPEEVFLSKGWAKILVALHVGTLAFCA-- 257
EED91209            215 LLGTIRHLSVCASVQLALGWPFLTTYPISYVKKAFEFDRVFFFMWTVNWKFLPEEMFVSKPWALVLLLCHLGTLALLA-- 292
WGS:AAPU:GI19772-PA 310 TWTFFKSY--------------V---HLREVQQIILpqlmlknreekekakaakkkshhrskskksqqqeqaqelepgnk 372
XP_003063992        261 HARWYRRGggffpaffvdfferA---RTDAAASKRLk------------------------------------------- 294
jgi:OSTLU_47741     288 HRRWCAREggfffnfvrdffkrLllrNVDGVQST---------------------------------------------- 321
XP_001568847        271 RMRWAPRT----------------------YLTDAEarvlhpaiadtrrqntss-------------------------- 302
Q4Q1L8              271 RTRWAART----------------------YLTDAEaqvlhpaisdtrrqntpl-------------------------- 302 Leishman...
Q4E4B0              265 RRRWSRRL----------------------FRRAQVatiksveyga---------------------------------- 288 Trypanos...
Q38AV3              265 FRRWSKRA----------------------YRRGRCekregtv------------------------------------- 285 Trypanos...
CBJ29699            293 -SKWLRSA--------------R---EVETFSASDLragegagg------------------------------------ 318
XP_002178258        258 TQQLAQAK----------------------RKTSKQiflldqs------------------------------------- 278
EED91209            293 KKWWAASL----------------------SQKGEWmcwmksk------------------------------------- 313
WGS:AAPU:GI19772-PA 373 eedeeeltaeqksflksfekglqnatgqkrppapvkeskrkpyeisfEHCTQLALLPFFLCNFIGVVCARSLHYQFYVWY 452
XP_003063992        295 -----------------------------------------------SLTAADVVCAMAEGNFIGIVFARSLHYQFYAWY 327
jgi:OSTLU_47741     322 ------------------------------------------------FTPAHVALVLAQSNLIGIVFARSLHYQFYSWY 353
XP_001568847        303 ------------------------------llhavedeemmddaaqeQAVYASTLLTFFEANLVGVIFARTIHYQFYTWF 352
Q4Q1L8              303 ------------------------------rrgtadgdetmddeaqeQAVYAATLLTFFEANLVGVIFARSIHYQFYTWF 352 Leishman...
Q4E4B0              289 -------------------------------------vrgadvfdtdSEVYRNVVLTMMESNLIGVIFARSLHYQFLVWF 331 Trypanos...
Q38AV3              286 ----------------------------------------vlvgasdEEVFHNVTLTLMESNMIGVIFARSLHYQFFLWF 325 Trypanos...
CBJ29699            319 ---------------------------------------atggavnaRLHPEYVVKTLFVSNFIGIVFCRSLHYQFYSWY 359
XP_002178258        279 ----------------------------------------ssnqnarRISPEYVVYIMFLSNFIGIAFARTLHYQFYCWY 318
EED91209            314 -----------------------------------------snkqsvRLSPEYVIYTMFVSNYIGIVFARTLHYQFYCWY 352
WGS:AAPU:GI19772-PA 453 FHSLPYLV---------WST-----------------PY--SVGVRFLILGGIEYSFNTYPSTNLSS 491
XP_003063992        328 FHSLPLLL---------WSIssngsrrggkgggrlrrAL--AVAARVGAMGAIEWCWNVYPSTPTSS 383
jgi:OSTLU_47741     354 FHTIPLLL---------WSNp----------------RV--PVGAKLAIMGAIEWCWNVYPSTPTSS 393
XP_001568847        353 FYTVPFVL---------AYT-----------------TY--PWPLRIVSCALISQGFESFPPTPRTS 391
Q4Q1L8              353 FYMVPFVL---------AYT-----------------TF--PRVLRLVSFALIRQGFESFPPTPKTS 391 Leishmania major
Q4E4B0              332 FHSMPMVL---------SVT-----------------RL--PFPLQIASVVAVQYGFEAYPSTAQSS 370 Trypanosoma cruzi
Q38AV3              326 FYFVPFVL---------SAT-----------------RL--PLVVKVVAFLAIQYGFEVYPSTTASS 364 Trypanosoma brucei
CBJ29699            360 YHTLPFLL---------WQT-----------------GL--PVALRLVVLCGVEVGFNVFPATALSS 398
XP_002178258        319 FHGLPYLL---------WSIp----------------SY--PLLLKIVIMGCIEAAFLSFPATSWSS 358
EED91209            353 FYSLPMMHlmatitstmSAT-----------------SIflSTIATIVAIFGGEYAFNVFPATEQSS 402
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