3LRE


Conserved Protein Domain Family
KISc_KIP3_like

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cd01370: KISc_KIP3_like 
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Kinesin motor domain, KIP3-like subgroup
Kinesin motor domain, KIP3-like subgroup. The yeast kinesin KIP3 plays a role in positioning the mitotic spindle. This catalytic (head) domain has ATPase activity and belongs to the larger group of P-loop NTPases. Kinesins are microtubule-dependent molecular motors that play important roles in intracellular transport and in cell division. In most kinesins, the motor domain is found at the N-terminus (N-type). N-type kinesins are (+) end-directed motors, i.e. they transport cargo towards the (+) end of the microtubule. Kinesin motor domains hydrolyze ATP at a rate of about 80 per second, and move along the microtubule at a speed of about 6400 Angstroms per second. To achieve that, kinesin head groups work in pairs. Upon replacing ADP with ATP, a kinesin motor domain increases its affinity for microtubule binding and locks in place. Also, the neck linker binds to the motor domain, which repositions the other head domain through the coiled-coil domain close to a second tubulin dimer, about 80 Angstroms along the microtubule. Meanwhile, ATP hydrolysis takes place, and when the second head domain binds to the microtubule, the first domain again replaces ADP with ATP, triggering a conformational change that pulls the first domain forward.
Statistics
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PSSM-Id: 276821
Aligned: 13 rows
Threshold Bit Score: 490.704
Created: 1-Nov-2000
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
 
ATP bindingmicrotubule
Conserved site includes 8 residues -Click on image for an interactive view with Cn3D
Feature 1:ATP binding site [chemical binding site]
Evidence:
  • Comment:interaction site for Mg ADP, ATP hydrolysis drives motor function
  • Structure:3LRE; Homo sapiens Human Kinesin-8 Motor domain with bound ADP, contacts 4A

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1              #                                                                       
3LRE_A      11 HMKVVVRVRPentke---------------------------------------------------------kaagFHKV 33   human
AAH54210    11 HVRVVVRVRPeneke---------------------------------------------------------kqgnFSRV 33   African clawed frog
AAH44083    11 QLTVALRIRPinetelaeg-------------------------------------------------atiiahkvDKQM 41   African clawed frog
AAR39439    16 SMIVTVRIRPesqse--------------------------------------------------------ilnknCKTI 39   Dictyostelium di...
EAA21814   794 NMNVVIRCRPmsnse---------------------------------------------------------knegAKNV 816  Plasmodium yoeli...
BAC04386    11 QLMVALRVRPisvaeleeg-------------------------------------------------atliahkvDEQM 41   human
NP_956533    9 HVKVVVRVRPlndke---------------------------------------------------------kdgnFKKV 31   zebrafish
EAK82615     5 SINVCVRIRPfsekeasqlapnedhtpflgdggla----------------ggispnnflpstsaaatastlktrfVRPI 68   Ustilago maydis 521
NP_523992    8 NIKVAVRVRPynvre---------------------------------------------------------leqkQRSI 30   fruit fly
P53086      10 SIVVAIRVRPftsmektrlvneasgaeanfpglgdsslilpmsnnsdsdididaeegstrskrnsllrrkvirpegIRKI 89   Saccharomyces ce...
NP_996208   35 RLVVAVRVRPsm--------------------------------------------------------------eaTERC 52   fruit fly
AAM53316    14 TLTVAVKCRPlme------------------------------------------------------------kerGRDI 33   thale cress
O14343       6 SITVTVRVRPfstaesanliassdrl----------------------------------sfgtssslrnpgsgrqIRRV 51   fission yeast
Feature 1                                                                                      
3LRE_A      34 VHVVDk-HILVFDpkqeevsffhgk-----------------------kttnqnvikkqnKDLKFVFDAVFDEtSTQSEV 89   human
AAH54210    34 VQVVDn-HILVFDpkveevgffhgr-----------------------sranrditkrknKDLKFVFDCVFDDsSCQLEV 89   African clawed frog
AAH44083    42 VVLMD--PMEDSDdilr---------------------------------------anrsREKSYMFDVAFDYtATQDTV 80   African clawed frog
AAR39439    40 VRVIDd-NMLVFDpndidigafnn--------------------------nrnnkqsqqpVEQKYIFDRVFDQyATQEEV 92   Dictyostelium di...
EAA21814   817 IKIMDnkMIVLLDpsdntdn---------------------------------vlrqnrtKEKRYCFDYVFDEnNTQEDV 863  Plasmodium yoeli...
BAC04386    42 VVLMD--PMEDPDdilr---------------------------------------ahrsREKSYLFDVAFDFtATQEMV 80   human
NP_956533   32 VHVVDn-HMLVFDpkeeevtffrgq-----------------------rvgnrdvrkranKDLKFVFDSVFGEeSSQIEV 87   zebrafish
EAK82615    69 VKPMDq-KVLVFDppdtnpltrlyn------------------------snplarggrrnKDVRYAFDRVFDStASQTDV 123  Ustilago maydis 521
NP_523992   31 IKVMDr-SALLFDpdeeddefffqga----------------------kqpyrditkrmnKKLTMEFDRVFDIdNSNQDL 87   fruit fly
P53086      90 VDCVDd-RMLIFDpadrnplnkvsdqvlnsmraratkatassinnsnatnkfssqrrrhgGEIKFVFDKLFDEtSSQARV 168  Saccharomyces ce...
NP_996208   53 IEVISg-GSLLYDdgg------------------------------------------ksRPRQYSYDHVFREnDSQEQV 89   fruit fly
AAM53316    34 VRVNNskEVVVLDpdlskdyl--------------------------------driqnrtKEKKYCFDHAFGPeSTNKNV 81   thale cress
O14343      52 VKVLDg-RVLVFDppdettatlsatnrrlsts----------qqslarlsrksnnsagfgRDLRYAFDRVFDEtATQQQV 120  fission yeast
Feature 1                             #  # ####                                                
3LRE_A      90 FEHTTkPILRSFLNGYNCTVLAYGATGAGKTHTMLGSa------------------------dEPGVMYLTMLHLYKCMd 145  human
AAH54210    90 FEQTTkIVLDGVLNGYNCTVLAYGATGAGKTHTMLGSp------------------------hEPGVMYLTMMELYNRId 145  African clawed frog
AAH44083    81 YRFTTkGLIEGVISGYNATVFAYGPTGCGKTYTMLGTd------------------------wEPGIYIRTLNDLFKAIe 136  African clawed frog
AAR39439    93 FENTTkELVSYVISGHNASVFAYGASGAGKTHTMVGGin-----------------------tGPGIMVLTMKELFSLIe 149  Dictyostelium di...
EAA21814   864 YNNSVkPLVDAVIKGYNSTVFAYGATGAGKTHTIIGYk------------------------nEPGIMMMILQDLFKKIk 919  Plasmodium yoeli...
BAC04386    81 YQATTkSLIEGVISGYNATVFAYGPTGCGKTYTMLGTd------------------------qEPGIYVQTLNDLFRAIe 136  human
NP_956533   88 FENTTkAIVDGVLNGYNCTVFAYGATGAGKTHTMLGTs------------------------nSPGVMFLTMKELFARMd 143  zebrafish
EAK82615   124 FEETCkPLLDGILNGYNASVFAYGATGCGKTHTISGTp------------------------nDPGLIFLTMKELYNRIq 179  Ustilago maydis 521
NP_523992   88 FEECTaPLVDAVLNGYNCSVFVYGATGAGKTFTMLGSe------------------------aHPGLTYLTMQDLFDKIq 143  fruit fly
P53086     169 YKETTsPLLDSVLDGFNSTVFAYGATGCGKTYTVSGTp------------------------sQPGIIFLAMEELFNKIt 224  Saccharomyces ce...
NP_996208   90 YKTTTaPLVRDVLNGLNAAVFAYGATGSGKTHTMLGPvprkkpqtsdrapptascdstdvssqDIGLMVRAIEDIFSHIe 169  fruit fly
AAM53316    82 YRSMS-SVISSVVHGLNATVFAYGSTGSGKTYTMVGTr------------------------sDPGLMVLSLNTIFDMIk 136  thale cress
O14343     121 YERTArPLLDNILDGFNATIFAYGATGCGKTHTISGTm------------------------qDPGLIYLTLKELFERMd 176  fission yeast
Feature 1                                                                                      
3LRE_A     146 eikeekiCSTAVSYLEVYNEQIRDLLvn---------sgPLAVREdtq-kgVVVHGLTLHQPkSSEEILHLLDNGNKNRT 215  human
AAH54210   146 svkeekvCNVAISYLEVYNEQIRDLLsn---------sgQLAVREdaq-kgVVVQGLTLHQPkSAEEILQMLDVGNKNRT 215  African clawed frog
AAH44083   137 etsddmeYEVLMSYMEIYNEMIRDLLnps--------lgYLDLREdsk-gvIQVAGITEVSTiNAKEIMQLLMKGNRQRT 207  African clawed frog
AAR39439   150 kdr-snqYIVSMSYLEVYNETIRDLLitntggggnsnnkVLELCEden-kqIVIRDLSWEYPtSADQVFKLLKYGNLNRK 227  Dictyostelium di...
EAA21814   920 tlkamneYKIKCSFIEIYNENICDLLnps--------seYLDLREdpv-kgITVSNLFEVCTtSVEEIMELIHTGNRNRT 990  Plasmodium yoeli...
BAC04386   137 etsndmeYEVSMSYLEIYNEMIRDLLnps--------lgYLELREdsk-gvIQVAGITEVSTiNAKEIMQLLMKGNRQRT 207  human
NP_956533  144 likedkvFNVAFSYLEVYNEQIRDLLtn---------sgPLAVREdgs-ngVVVQGLTLHQPkSAEHILEALDYGNRNRT 213  zebrafish
EAK82615   180 darddsdVHIRLSYLEIYNETIRDLLssept----ppgaGLALREdan-nrISVVGITELIPeSPESVLEYIQEGNLRRT 254  Ustilago maydis 521
NP_523992  144 aqsdvrkFDVGVSYLEVYNEHVMNLLtk---------sgPLKLREdn--ngVVVSGLCLTPIySAEELLRMLMLGNSHRT 212  fruit fly
P53086     225 dlkdekdFEISLSYLEIYNERIRDLLkpet------pskRLVIREdtq-nhIKVANLSYHHPnTVEDVMDLVVQGNINRT 297  Saccharomyces ce...
NP_996208  170 sae-aysCRVSISYLEIYNELIRDLLnp---------ggPLELREdhrgqrITVAGLSEITTsSRKEVVSLLLKGNKART 239  fruit fly
AAM53316   137 sdkssdeFEVTCSYLEVYNEVIYDLLeks--------sgHLELREdpe-qgIVVAGLRSIKVhSADRILELLNLGNSRRK 207  thale cress
O14343     177 hlrdekiFDLRLSYLEIYNETIRDLLvsptp----nqakPLNLREdad-rrITVPGLTSLSPeSLEEIIDIIMKGNANRT 251  fission yeast
Feature 1                                                #                                     
3LRE_A     216 QHPTDMNATSSRSHAVFQIYLRqqdktasinqnvrIAKMSLIDLAGSERASTSgaKGTRFVEGTNINRSLLALGNVINAL 295  human
AAH54210   216 QHPTDMNASSSRSHAVFQIYLRqqdktasinqnvrIAKMTLIDLAGSERASATnaKGDRLREGTNINRSLLALGNVINAL 295  African clawed frog
AAH44083   208 QEPTAANKTSSRSHAILQVTVRqksrvknitqevrVGRLFMIDLAGSERASQTqnRGLRMKEGAHINRSLLALGNCINAL 287  African clawed frog
AAR39439   228 QSPTQTNQTSSRSHAVLQITVKqqnlqd--kskisFGKLSLIDLAGSERASKTlnTGDRLKEGTSINKSLLALGNCIKAL 305  Dictyostelium di...
EAA21814   991 QEPTDANKTSSRSHGVLQVTVEetekgqglyqqtkKGKLCVIDLAGSERASQTnnKGMRMLEGANINRSLLALGNVINAL 1070 Plasmodium yoeli...
BAC04386   208 QEPTAANQTSSRSHAVLQVTVRqrsrvknilqevrQGRLFMIDLAGSERASQTqnRGQRMKEGAHINRSLLALGNCINAL 287  human
NP_956533  214 QHPTDMNATSSRSHAVFQIYLRqqdktaslnpnvrVAKMSLIDLAGSERASATntKGARLREGANINRSLLALGNVINTL 293  zebrafish
EAK82615   255 MSPTEANAVSSRSHAVLQINVTqkprtasmieqttSASLNIIDLAGSERASATrnNGARMKEGANINKSLLALGNCINAL 334  Ustilago maydis 521
NP_523992  213 QHPTDANAESSRSHAIFQVHIRiterk---tdtkrTVKLSMIDLAGSERAASTkgIGVRFKEGASINKSLLALGNCINKL 289  fruit fly
P53086     298 TSPTEANEVSSRSHAVLQIHIMqtnklvdltsqhtFATLSIIDLAGSERAAATrnRGIRLHEGANINRSLLALGNCINAL 377  Saccharomyces ce...
NP_996208  240 MEPTAANQTSSRSHALLSIMVQartp-----lgtkQGRLFLTDLAGSERAKKTknRGKRLQEGAHINRSLLALGNVINGL 314  fruit fly
AAM53316   208 TESTEMNGTSSRSHAVLEIAVKrrqkn---qnqvmRGKLALVDLAGSERAAETnnGGQKLRDGANINRSLLALANCINAL 284  thale cress
O14343     252 MSPTEANAASSRSHAVLQVTLIqkprtaginedhtLATLSIIDLAGSERATATklRGSRLFEGANINKSLLALGNCINAL 331  fission yeast
Feature 1                                                                           
3LRE_A     296 ADskr---------knqHIPYRNSKLTRLLKDSLGGNCQTIMIAAVSPSSVFYDDTYNTLKYANRAKDI 355  human
AAH54210   296 ADpks---------kkqHIPYRNSKLTRLLKDSLGGNCRTIMIAAVSPSSLSYDDTYNTLKYANRAKDI 355  African clawed frog
AAH44083   288 SErgs----------nkYVNYRDSKLTRLLKDSLGGNSRTVMIAHISPASTSFEESRNTLTYADRAKNI 346  African clawed frog
AAR39439   306 GElcknqqsqqqssnpnFIPYRDSKLTRILKDSLTGSCKTIMIANISPNSSSFEETHNTLKYAQRAKSI 374  Dictyostelium discoideum
EAA21814  1071 VSrskgt------sksnFIPFRDSKLTRLLKDSLGGNCKTLMIANISPSHLSYEDTHNTLKYANRAKNI 1133 Plasmodium yoelii yoelii
BAC04386   288 SDkgs----------nkYINYRDSKLTRLLKDSLGGNSRTVMIAHISPASSAFEESRNTLTYAGRAKNI 346  human
NP_956533  294 ANpkc---------kktHIPYRDSKLTRLLKDSLGGNCRTVMIANVSPSSLSYEDTHNTLKYANRAKEI 353  zebrafish
EAK82615   335 CQsgg---------vvrHVPYRNSKLTRLLKFSLGGNCKTVMVVCISPSSTHYEETHNALKYANQAKNI 394  Ustilago maydis 521
NP_523992  290 ADgl------------kHIPYRDSNLTRILKDSLGGNCRTLMVANVSMSSLTYEDTYNTLKYASRAKKI 346  fruit fly
P53086     378 CLndgs--------rscHIPYRDSKLTRLLKFSLGGNCKTVMIVCISPSSSHYDETLNTLKYANRAKEI 438  Saccharomyces cerevisiae S288c
NP_996208  315 SGga------------rYVNYRDSKLTRLLKEALSGRCKTVMIAHVAPESKHRDETKNTLVYADRANSI 371  fruit fly
AAM53316   285 GKqhkk--------glaYVPYRNSKLTRILKDGLSGNSQTVMVATISPADSQYHHTVNTLKYADRAKEI 345  thale cress
O14343     332 CDphr----------raHVPYRDSKLTRLLKFSLGGNCRTVMIVCVSPSSVHYEETHNTLKYANRAKNI 390  fission yeast

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