1UAD,1U90,2BOV,2A9K


Conserved Protein Domain Family
RalA_RalB

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cd04139: RalA_RalB 
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Ral (Ras-like) family containing highly homologous RalA and RalB
The Ral (Ras-like) subfamily consists of the highly homologous RalA and RalB. Ral proteins are believed to play a crucial role in tumorigenesis, metastasis, endocytosis, and actin cytoskeleton dynamics. Despite their high sequence similarity (>80% sequence identity), nonoverlapping and opposing functions have been assigned to RalA and RalBs in tumor migration. In human bladder and prostate cancer cells, RalB promotes migration while RalA inhibits it. A Ral-specific set of GEFs has been identified that are activated by Ras binding. This RalGEF activity is enhanced by Ras binding to another of its target proteins, phosphatidylinositol 3-kinase (PI3K). Ral effectors include RLIP76/RalBP1, a Rac/cdc42 GAP, and the exocyst (Sec6/8) complex, a heterooctomeric protein complex that is involved in tethering vesicles to specific sites on the plasma membrane prior to exocytosis. In rat kidney cells, RalB is required for functional assembly of the exocyst and for localizing the exocyst to the leading edge of migrating cells. In human cancer cells, RalA is required to support anchorage-independent proliferation and RalB is required to suppress apoptosis. RalA has been shown to localize to the plasma membrane while RalB is localized to the intracellular vesicles. Most Ras proteins contain a lipid modification site at the C-terminus, with a typical sequence motif CaaX, where a = an aliphatic amino acid and X = any amino acid. Lipid binding is essential for membrane attachment, a key feature of most Ras proteins. Due to the presence of truncated sequences in this CD, the lipid modification site is not available for annotation.
Statistics
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PSSM-Id: 206710
Aligned: 11 rows
Threshold Bit Score: 228.464
Created: 1-Sep-2005
Updated: 2-Oct-2020
Structure
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Program:
Drawing:
Aligned Rows:
  next features
Conserved site includes 16 residues -Click on image for an interactive view with Cn3D
Feature 1:GTP/Mg2+ binding site [chemical binding site]
Evidence:
  • Structure:1UAD: Human RalA binds GppNHp, a GTP analog and Mg2+, defined using 3.5 A contacts

Sequence Alignment
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Format: Row Display: Color Bits: Type Selection:
Feature 1             #######                  ##                   #                        #
1UAD_A      7 HKVIMVGSGGVGKSALTLQFm---------YDEFVe--------------dYEPTKADSYRKKVVLDgEEVQIDILDTAG 63  human
1U90_B      5 HKVIMVGSGGVGKSALTLQFm---------YDEFVe--------------dYEPTKADSYRKKVVLDgEEVQIDILDTAG 61  cotton-top tamarin
2BOV_A     15 HKVIMVGSGGVGKSALTLQFm---------YDEFVe--------------dYEPTKADSYRKKVVLDgEEVQIDILDTAG 71  human
2A9K_A     19 HKVIMVGSGGVGKSALTLQFm---------YDEFVe--------------dYEPTKADSYRKKVVLDgEEVQIDILDTAG 75  human
AAH53216   15 HKVIMVGSGGVGKSALTLQFm---------YDEFVe--------------dYEPTKADSYRKKVVLDgEEVQIDILDTAG 71  zebrafish
CAE18159   11 VKLIVAGVGGVGKSALTLYFm---------YDEFVk--------------dYEPTRADSYRKKISFGsEDVQLHILDTAG 67  Echinococcus multi...
CAA76143   15 HKVIMVGSGGVGKSALTLQFm---------YDEFVe--------------dYEPTKADSYRKKVVLDgEEVQIDILDTAG 71  African clawed frog
AAN09102   12 HKVIMVGSGGVGKSALTLQFm---------YDEFVe--------------dYEPTKADSYRKKVVLDgEEVQIDILDTAG 68  fruit fly
AAW26306    7 FKVALIGIGGVGKSALTLQFm---------YEEFVe--------------eYEPTKADSYRKTINVDgEQIQLNILDTAG 63  Schistosoma japonicum
P24498      4 YKIVIVGGGLVGKSALTLQLvqvcikdqyyLIEFQnnqfqfenlqnhyideYDPTVEDSRREVSIDD-QTCLLNILDTAG 82  Geodia cydonium
BAC76997   42 YKAVVVGASGVGKSALTIQLn---------HQCFVe--------------dHDPTIQDSYWKELTLDsGDCILNVLDTAG 98  human
Feature 1                                                              ## #                   
1UAD_A     64 QEDYAAIRDNYFRSGEGFLCVFSITEMESFAATADFREQILRvke--deNVPFLLVGNKSDLEd-kRQVSVEEAKNRAEQ 140 human
1U90_B     62 QEDYAAIRDNYFRSGEGFLCVFSITEMESFAATADFREQILRvke--deNVPFLLVGNKSDLEd-kRQVSVEEAKNRADQ 138 cotton-top tamarin
2BOV_A     72 QEDYAAIRDNYFRSGEGFLCVFSITEMESFAATADFREQILRvke--deNVPFLLVGNKSDLEd-kRQVSVEEAKNRAEQ 148 human
2A9K_A     76 QEDYAAIRDNYFRSGEGFLCVFSITEMESFAATADFREQILRvke--deNVPFLLVGNKSDLEd-kRQVSVEEAKNRAEQ 152 human
AAH53216   72 QEDYAAIRDNYFRSGEGFLCVFSITELESFAATADFREQILRvke--deNVPFLLVGNKSDLEd-rRQVSADEAKARADQ 148 zebrafish
CAE18159   68 QEDYAGIRDTFYRNSEGFLLVFDLTDRQSFTSLGEFVDQILRvkr--sdRVPMLICGNKVDLTe-nRRVSQEEAEDFCRK 144 Echinococcus multi...
CAA76143   72 QEDYAAIRDNYFRSGEGFLLVFSITEHESFTATAEFREQILRvka-eedKIPLLIVGNKSDLEd-rRQVPMDEARGKAEE 149 African clawed frog
AAN09102   69 QEDYAAIRDNYFRSGEGFLCVFSITDDESFQATQEFREQILRvkn--deSIPFLLVGNKCDLNd-kRKVPLSECQLRAQQ 145 fruit fly
AAW26306   64 QESYGGVMESCMRSCDGFLLVYSVDDEESVTVLSELYERVIRvkn--ndQVPLIVVANKCDLTgphIQKYIDEGANFAVH 141 Schistosoma japonicum
P24498     83 QQHSNAQSMDAHWSTVFVCLFNYFNITSMYDEIASFREQILRvkdgakdLVPLILIINKADLDh--ESQGSGNEGQLAKD 160 Geodia cydonium
BAC76997   99 QAIHRALRDQCLAVCDGVLGVFALDDPSSLIQLQQIWATWGPh-----pAQPLVLVGNKCDLVt-tAGDAHAAAAALAHS 172 human
Feature 1             ##                   
1UAD_A    141 WnVNYVETSAKTRANVDKVFFDLMREIRA 169 human
1U90_B    139 WnVNYVETSAKTRANVDKVFFDLMREIRA 167 cotton-top tamarin
2BOV_A    149 WnVNYVETSAKTRANVDKVFFDLMREIRA 177 human
2A9K_A    153 WnVNYVETSAKTRANVDKVFFDLMREIRA 181 human
AAH53216  149 WgVCYVETSAKTRANVDKVFFDLMREIRA 177 zebrafish
CAE18159  145 ChVPYLETSAKTNTNVEKAFLTLAREVYQ 173 Echinococcus multilocularis
CAA76143  150 WgVQYVETSAKTRANVDKVFFDLMREIRT 178 African clawed frog
AAN09102  146 WaVPYVETSAKTRENVDKVFYDLIRDISS 174 fruit fly
AAW26306  142 YkVQHIRTSAKTCLNVDKIFTEICREIRQ 170 Schistosoma japonicum
P24498    161 S-LSFHQSSAKSRINLEEIPYSLVRELRK 188 Geodia cydonium
BAC76997  173 WgAHFVETSAKTRQGVEEAFSLLVHEIQR 201 human

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