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WHO Guidelines on Drawing Blood: Best Practices in Phlebotomy. Geneva: World Health Organization; 2010.
Before undertaking phlebotomy, health workers should be trained in, and demonstrate proficiency for, the blood collection procedures on the patient population that will be within their scope of practice.
Training should cover paediatric, neonatal and intensive care, and blood transfusion, as appropriate.
Competence in phlebotomy practices should be an essential part of the final evaluation of those training as health workers.
The outcome of the course should be safety of patients, adequacy of the laboratory specimens, and safety of health workers and the community.
Course contents
- Anatomy of the phlebotomy sites from which the worker is authorized to access blood.
- Infection prevention and control:
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elements of standard precautions relevant to venepuncture (hand hygiene, wearing non-sterile gloves);
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use of antiseptics – skin disinfection;
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cleaning and disinfection of materials used on more than one patient, including tourniquets, scissors and specimen carriers;
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disposal of used equipment, especially sharps.
- Protection of the patient:
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patient identification, including children and confused patients;
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awareness of the institution's rule to halt and seek help after a defined number of unsuccessful draws;
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informed consent and patient rights;
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managing supplies for patients in isolation;
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awareness of contraindications to blood draws including drawing on the same side as a mastectomy, through infected or scarred tissues, and through in-dwelling vascular devices (per institutional policy).
- Protection of the health worker:
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immunization with hepatitis B;
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awareness of high risk devices and practices;
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ability to state who and when to contact for support in the event of exposure to blood and body fluids;
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awareness of the benefits of PEP and the need to have source patients tested and HIV PEP started, preferably within hours;
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avoidance of two-handed needle recapping, disassembly of devices, removal of needles prior to injecting blood into tube;
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placement and use of sharps container within arm's reach;
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appropriate use of personal protective equipment, including gloves.
- Types of equipment available for blood sampling, and procurement and use of equipment.
- Practice taking blood samples, including blood sampling and simulated blood sampling (capillary blood, arterial blood, venous blood from adults and children according to responsibilities.
- Practice on artificial arms and clinical skills development.
- Special techniques:
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capillary puncture
- heel and finger-pricks
- lancets
- capillary tubes (filter paper, capillary blood tubes, rapid test strips, etc.)
- –
venous blood
- large volume (blood letting – aware that this must be done under direct physician order and management)
- winged needles
- evacuated tubes
- blood cultures.
- Adverse events and management.
- Occupational exposure and management:
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the country's relevant occupational health regulations, including PEP for prevention of HIV and hepatitis B;
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the procedure for, and benefits of, reporting occupational exposure to blood;
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first aid after exposure (see management);
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PEP (importance of timely response);
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surveillance and use of data for prevention of occupational exposure.
- Waste management, including disposal of waste and sharps, and procedures for spillage and breakage.
- Laboratory practices, including type of samples, forms, labelling and transportation.
- Standards of practice.
- Sustainability of training programme.
- Career path.
- Skill-based incentives.
- Training course content for phlebotomists - WHO Guidelines on Drawing BloodTraining course content for phlebotomists - WHO Guidelines on Drawing Blood
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