CorrectionThe following RGB settings were added: “*Note: To reproduce precise colors in RGB format, use the following triplet values: Galactose stereochemistry: Yellow (255,255,0); Glucose stereochemistry: BLUE (0,0,250); Mannose stereochemistry: GREEN (0,200,50); Fucose: RED (250,0,0); Xylose: ORANGE (250,100,0); Neu5Ac: PURPLE (125,0,125); Neu5Gc: LIGHT BLUE (200,250,250); KDN: GREEN (0,200,50); GlcA: BLUE (0,0,250); IdoA: TAN (150,100,50); GalA: Yellow (255,255,0); ManA: GREEN (0,200,50).”
Recommended symbols and conventions for drawing glycan structures.
(Top panel) The monosaccharide symbol set from the
first edition of Essentials of Glycobiology is modified to
avoid using the same shape or color, but with different orientation to
represent different sugars. Each monosaccharide class (e.g., hexose) now has
the same shape, and isomers are differentiated by
color/black/white/shading. The same shading/color is
used for different monosaccharides of the same stereochemical designation,
e.g., Gal, GalNAc, and GalA. To minimize variations, sialic acids and uronic
acids are in the same shape, and only the major uronic and sialic acid types
are represented. When the type of sialic acid is uncertain, the abbreviation
Sia can be used instead. Only common monosaccharides in vertebrate systems
are assigned specific symbols. All other monosaccharides are represented by
an open hexagon or defined in the figure legend. If there is more than one
type of undesignated monosaccharide in a figure, a letter designation can be
included to differentiate between them. Unless otherwise indicated, all of
these vertebrate monosaccharides are assumed to be in the D configuration
(except for fucose and iduronic acid, which are in the L configuration), all
glycosidically linked monosaccharides are assumed to be in the pyranose
form, and all glycosidic linkages are assumed to originate from the
1-position (except for the sialic acids, which are linked from the
2-position). Anomeric notation and destination linkages can be indicated
without spacing/dashes. Although color is useful, these representations will
survive black-and-white printing or photocopying with the colors represented
in different shades (the color values in the figure are the RGB triplet
color settings)*. Modifications of monosaccharides are indicated by
lowercase letters, with numbers indicating linkage positions, if known
(e.g., 9Ac for the 9-O-acetyl group, 3S for the 3-O-sulfate group, 6P for a
6-O-phosphate group, 8Me for the 8-O-methyl group, 9Acy for the 9-O-acyl
group, and 9Lt for the 9-O-lactyl group). Esters and ethers are shown
attached to the symbol with a number. For N-substituted groups, it is
assumed that only one amino group is on the monosaccharide with an already
known position (e.g., NS for an N-sulfate group on glucosamine, assumed to
be at the 2-position). (Middle panel) Typical branched
“biantennary” N-glycan with two types of outer
termini, depicted at different levels of structural details. (Bottom
panel) Some typical glycosaminoglycan (GAG) chains.
*Note: To reproduce precise colors in RGB format, use the following triplet
values: Galactose stereochemistry: Yellow (255,255,0); Glucose
stereochemistry: BLUE (0,0,250); Mannose stereochemistry: GREEN (0,200,50);
Fucose: RED (250,0,0); Xylose: ORANGE (250,100,0); Neu5Ac: PURPLE
(125,0,125); Neu5Gc: LIGHT BLUE (200,250,250); KDN: GREEN (0,200,50); GlcA:
BLUE (0,0,250); IdoA: TAN (150,100,50); GalA: Yellow (255,255,0); ManA:
GREEN (0,200,50).